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Protein

Leukosialin

Gene

Spn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1. During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response.1 Publication

GO - Biological processi

  • apoptotic signaling pathway Source: MGI
  • cell surface receptor signaling pathway Source: MGI
  • defense response to bacterium Source: MGI
  • negative regulation of cell adhesion Source: MGI
  • negative regulation of T cell activation Source: MGI
  • negative regulation of T cell proliferation Source: MGI
  • negative regulation of type IV hypersensitivity Source: MGI
  • negative thymic T cell selection Source: MGI
  • positive regulation of protein phosphorylation Source: MGI
  • positive regulation of T cell proliferation Source: MGI
  • positive regulation of tumor necrosis factor biosynthetic process Source: MGI
  • regulation of defense response to virus Source: MGI
  • regulation of immune response Source: MGI
  • response to protozoan Source: MGI
  • T cell costimulation Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-202733. Cell surface interactions at the vascular wall.
R-MMU-210991. Basigin interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Leukosialin
Alternative name(s):
B-cell differentiation antigen LP-3
Leukocyte sialoglycoprotein
Lymphocyte antigen 48
Short name:
Ly-48
Sialophorin
CD_antigen: CD43
Gene namesi
Name:Spn
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:98384. Spn.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 248229ExtracellularSequence analysisAdd
BLAST
Transmembranei249 – 27123HelicalSequence analysisAdd
BLAST
Topological domaini272 – 395124CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • basement membrane Source: MGI
  • cell surface Source: MGI
  • external side of plasma membrane Source: MGI
  • extracellular exosome Source: MGI
  • extracellular space Source: MGI
  • integral component of plasma membrane Source: MGI
  • membrane Source: MGI
  • uropod Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Add
BLAST
Chaini20 – 395376LeukosialinPRO_0000021589Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi167 – 1671N-linked (GlcNAc...)Sequence analysis
Modified residuei285 – 2851PhosphoserineBy similarity
Modified residuei339 – 3391PhosphoserineCombined sources
Modified residuei343 – 3431PhosphoserineCombined sources
Modified residuei347 – 3471PhosphoserineCombined sources
Modified residuei371 – 3711PhosphoserineCombined sources
Modified residuei378 – 3781PhosphothreonineCombined sources

Post-translational modificationi

Has a high content of sialic acid and O-linked carbohydrate structures.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP15702.
PaxDbiP15702.
PRIDEiP15702.

PTM databases

iPTMnetiP15702.
PhosphoSiteiP15702.

Expressioni

Tissue specificityi

Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.

Gene expression databases

BgeeiP15702.
CleanExiMM_SPN.
ExpressionAtlasiP15702. baseline and differential.
GenevisibleiP15702. MM.

Interactioni

Subunit structurei

Interacts with HIPK2 via the cytoplasmic domain. Interacts with RDX.3 Publications

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000049534.

Structurei

Secondary structure

1
395
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi280 – 2834Combined sources
Turni285 – 2884Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2EMSX-ray2.90B272-291[»]
ProteinModelPortaliP15702.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15702.

Family & Domainsi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410J161. Eukaryota.
ENOG410YXQE. LUCA.
HOGENOMiHOG000294199.
HOVERGENiHBG006258.
InParanoidiP15702.
KOiK06477.
OMAiGPPLTMA.
OrthoDBiEOG7HXCVB.
PhylomeDBiP15702.
TreeFamiTF337688.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15702-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALHLLLLFG ACWVQVASPD SLQRTTMLPS TPHITAPSTS EAQNASPSVS
60 70 80 90 100
VGSGTVDSKE TISPWGQTTI PVSLTPLETT ELSSLETSAG ASMSTPVPEP
110 120 130 140 150
TASQEVSSKT SALLPEPSNV ASDPPVTAAN PVTDGPAANP VTDGTAASTS
160 170 180 190 200
ISKGTSAPPT TVTTSSNETS GPSVATTVSS KTSGPPVTTA TGSLGPSSEM
210 220 230 240 250
HGLPATTATS SVESSSVARG TSVSSRKTST TSTQDPITTR SPSQESSGML
260 270 280 290 300
LVPMLIALVV VLALVALLLL WRQRQKRRTG ALTLSGGGKR NGVVDAWAGP
310 320 330 340 350
ARVPDEEATT TSGAGGNKGS EVLETEGSGQ RPTLTTFFSR RKSRQGSLVL
360 370 380 390
EELKPGSGPN LKGEEEPLVG SEDEAVETPT SDGPQAKDEA APQSL
Length:395
Mass (Da):40,038
Last modified:April 1, 1990 - v1
Checksum:i369F201B04DBC055
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17018 Genomic DNA. Translation: CAA34884.1.
X52609 Genomic DNA. Translation: CAA36840.1.
S70677 mRNA. Translation: AAB30765.1.
M30693 mRNA. Translation: AAA39457.1.
CCDSiCCDS21858.1.
PIRiA43545.
RefSeqiNP_001032899.1. NM_001037810.2.
NP_033285.1. NM_009259.5.
XP_006507585.1. XM_006507522.2.
UniGeneiMm.283714.

Genome annotation databases

EnsembliENSMUST00000049931; ENSMUSP00000049534; ENSMUSG00000051457.
ENSMUST00000143713; ENSMUSP00000122787; ENSMUSG00000051457.
GeneIDi20737.
KEGGimmu:20737.
UCSCiuc009juh.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17018 Genomic DNA. Translation: CAA34884.1.
X52609 Genomic DNA. Translation: CAA36840.1.
S70677 mRNA. Translation: AAB30765.1.
M30693 mRNA. Translation: AAA39457.1.
CCDSiCCDS21858.1.
PIRiA43545.
RefSeqiNP_001032899.1. NM_001037810.2.
NP_033285.1. NM_009259.5.
XP_006507585.1. XM_006507522.2.
UniGeneiMm.283714.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2EMSX-ray2.90B272-291[»]
ProteinModelPortaliP15702.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000049534.

PTM databases

iPTMnetiP15702.
PhosphoSiteiP15702.

Proteomic databases

EPDiP15702.
PaxDbiP15702.
PRIDEiP15702.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000049931; ENSMUSP00000049534; ENSMUSG00000051457.
ENSMUST00000143713; ENSMUSP00000122787; ENSMUSG00000051457.
GeneIDi20737.
KEGGimmu:20737.
UCSCiuc009juh.2. mouse.

Organism-specific databases

CTDi6693.
MGIiMGI:98384. Spn.

Phylogenomic databases

eggNOGiENOG410J161. Eukaryota.
ENOG410YXQE. LUCA.
HOGENOMiHOG000294199.
HOVERGENiHBG006258.
InParanoidiP15702.
KOiK06477.
OMAiGPPLTMA.
OrthoDBiEOG7HXCVB.
PhylomeDBiP15702.
TreeFamiTF337688.

Enzyme and pathway databases

ReactomeiR-MMU-202733. Cell surface interactions at the vascular wall.
R-MMU-210991. Basigin interactions.

Miscellaneous databases

EvolutionaryTraceiP15702.
PROiP15702.
SOURCEiSearch...

Gene expression databases

BgeeiP15702.
CleanExiMM_SPN.
ExpressionAtlasiP15702. baseline and differential.
GenevisibleiP15702. MM.

Family and domain databases

ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Protein sequence and gene structure for mouse leukosialin (CD43), a T lymphocyte mucin without introns in the coding sequence."
    Cyster J.G., Somoza C., Killeen N., Williams A.F.
    Eur. J. Immunol. 20:875-881(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DBA/2J.
    Tissue: Liver.
  2. "The nucleotide sequence of Ly 48 (mouse leukosialin, sialophorin): the mouse homolog of CD43."
    Dorfman K.S., Litaker K.S., Baecher C.M., Frelinger J.G.
    Nucleic Acids Res. 18:4932-4932(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: B10.P.
    Tissue: Liver.
  3. "A unique murine CD43 epitope Lp-3: distinct distribution from another CD43 epitope S7."
    Shiota J., Nishimura H., Okamoto H., Yu B., Hattori S., Abe M., Okada T., Nozawa S., Tsurui H., Hirose S.
    Cell. Immunol. 155:402-413(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
  4. "cDNA cloning and localization of the mouse leukosialin gene (Ly48) to chromosome 7."
    Baecher C.M., Dorfman K.S., Mattei M.-G., Frelinger J.G.
    Immunogenetics 31:307-314(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 345-383.
    Strain: C57BL/6J.
  5. "Identification and cloning of a CD43-associated serine/threonine kinase."
    Wang W., Link V., Green J.M.
    Cell. Immunol. 205:34-39(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HIPK2.
  6. "CD43 functions as a T cell counterreceptor for the macrophage adhesion receptor sialoadhesin (Siglec-1)."
    van den Berg T.K., Nath D., Ziltener H.J., Vestweber D., Fukuda M., van Die I., Crocker P.R.
    J. Immunol. 166:3637-3640(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SN.
  7. "ERM-dependent movement of CD43 defines a novel protein complex distal to the immunological synapse."
    Allenspach E.J., Cullinan P., Tong J., Tang Q., Tesciuba A.G., Cannon J.L., Takahashi S.M., Morgan R., Burkhardt J.K., Sperling A.I.
    Immunity 15:739-750(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-339; SER-343; SER-347; SER-371 AND THR-378, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Lung and Spleen.
  9. "Structural basis of the cytoplasmic tail of adhesion molecule CD43 and its binding to ERM proteins."
    Takai Y., Kitano K., Terawaki S., Maesaki R., Hakoshima T.
    J. Mol. Biol. 381:634-644(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF 272-291 IN COMPLEX WITH RDX.

Entry informationi

Entry nameiLEUK_MOUSE
AccessioniPrimary (citable) accession number: P15702
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: June 8, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.