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Protein

Intestinal-type alkaline phosphatase 1

Gene

Alpi

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation2 Publications

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi62Magnesium1 Publication1
Metal bindingi62Zinc 11 Publication1
Active sitei112Phosphoserine intermediatePROSITE-ProRule annotation1 Publication1
Metal bindingi112Zinc 11 Publication1
Metal bindingi175Magnesium1 Publication1
Metal bindingi331Magnesium1 Publication1
Metal bindingi336Zinc 21 Publication1
Metal bindingi340Zinc 2; via tele nitrogen1 Publication1
Metal bindingi377Zinc 11 Publication1
Metal bindingi378Zinc 1; via tele nitrogen1 Publication1
Metal bindingi452Zinc 2; via tele nitrogen1 Publication1

GO - Molecular functioni

  • alkaline phosphatase activity Source: UniProtKB
  • magnesium ion binding Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • dephosphorylation Source: UniProtKB
  • phosphorus metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

SABIO-RKP15693.

Names & Taxonomyi

Protein namesi
Recommended name:
Intestinal-type alkaline phosphatase 1 (EC:3.1.3.1)
Short name:
IAP-1
Short name:
Intestinal alkaline phosphatase 1
Alternative name(s):
Intestinal alkaline phosphatase I
Short name:
IAP-I
Gene namesi
Name:Alpi
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2099. Alpi.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 201 PublicationAdd BLAST20
ChainiPRO_000002404121 – 511Intestinal-type alkaline phosphatase 1Add BLAST491
PropeptideiPRO_0000024042512 – 540Removed in mature formSequence analysisAdd BLAST29

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi141 ↔ 2031 Publication
Glycosylationi142N-linked (GlcNAc...)Sequence analysis1
Glycosylationi301N-linked (GlcNAc...)1 Publication1
Glycosylationi428N-linked (GlcNAc...)1 Publication1
Disulfide bondi487 ↔ 4941 Publication
Lipidationi511GPI-anchor amidated asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiP15693.
PRIDEiP15693.

Interactioni

Subunit structurei

Homodimer.1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026190.

Chemistry databases

BindingDBiP15693.

Structurei

Secondary structure

1540
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi25 – 27Combined sources3
Helixi29 – 45Combined sources17
Beta strandi54 – 61Combined sources8
Helixi66 – 79Combined sources14
Helixi91 – 93Combined sources3
Beta strandi95 – 101Combined sources7
Beta strandi105 – 109Combined sources5
Helixi112 – 121Combined sources10
Beta strandi130 – 132Combined sources3
Helixi141 – 144Combined sources4
Helixi152 – 158Combined sources7
Beta strandi162 – 170Combined sources9
Helixi174 – 177Combined sources4
Turni178 – 180Combined sources3
Helixi191 – 193Combined sources3
Helixi196 – 200Combined sources5
Helixi206 – 212Combined sources7
Beta strandi217 – 222Combined sources6
Helixi224 – 227Combined sources4
Helixi241 – 243Combined sources3
Beta strandi247 – 249Combined sources3
Helixi253 – 258Combined sources6
Beta strandi264 – 267Combined sources4
Helixi270 – 278Combined sources9
Beta strandi284 – 288Combined sources5
Beta strandi290 – 293Combined sources4
Helixi297 – 299Combined sources3
Turni302 – 304Combined sources3
Helixi308 – 319Combined sources12
Beta strandi326 – 332Combined sources7
Helixi335 – 338Combined sources4
Helixi344 – 364Combined sources21
Turni367 – 369Combined sources3
Beta strandi370 – 378Combined sources9
Beta strandi379 – 384Combined sources6
Beta strandi409 – 416Combined sources8
Helixi431 – 434Combined sources4
Beta strandi443 – 446Combined sources4
Beta strandi457 – 463Combined sources7
Helixi466 – 468Combined sources3
Beta strandi471 – 474Combined sources4
Helixi477 – 485Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4KJDX-ray2.21A/B21-502[»]
4KJGX-ray2.38A/B21-502[»]
ProteinModelPortaliP15693.
SMRiP15693.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal, Transmembrane

Phylogenomic databases

eggNOGiKOG4126. Eukaryota.
COG1785. LUCA.
HOGENOMiHOG000099118.
HOVERGENiHBG007345.
InParanoidiP15693.
PhylomeDBiP15693.

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15693-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQGDWVLLLL LGLRIHLSFG VIPVEEENPV FWNQKAKEAL DVAKKLQPIQ
60 70 80 90 100
TSAKNLILFL GDGMGVPTVT ATRILKGQLG GHLGPETPLA MDHFPFTALS
110 120 130 140 150
KTYNVDRQVP DSAGTATAYL CGVKANYKTI GVSAAARFNQ CNSTFGNEVF
160 170 180 190 200
SVMHRAKKAG KSVGVVTTTR VQHASPAGTY AHTVNRDWYS DADMPSSALQ
210 220 230 240 250
EGCKDIATQL ISNMDIDVIL GGGRKFMFPK GTPDPEYPGD SDQSGVRLDS
260 270 280 290 300
RNLVEEWLAK YQGTRYVWNR EQLMQASQDP AVTRLMGLFE PTEMKYDVNR
310 320 330 340 350
NASADPSLAE MTEVAVRLLS RNPQGFYLFV EGGRIDQGHH AGTAYLALTE
360 370 380 390 400
AVMFDSAIEK ASQLTNEKDT LTLITADHSH VFAFGGYTLR GTSIFGLAPL
410 420 430 440 450
NAQDGKSYTS ILYGNGPGYV LNSGNRPNVT DAESGDVNYK QQAAVPLSSE
460 470 480 490 500
THGGEDVAIF ARGPQAHLVH GVQEQNYIAH VMAFAGCLEP YTDCGLAPPA
510 520 530 540
DENRPTTPVQ NSAITMNNVL LSLQLLVSML LLVGTALVVS
Length:540
Mass (Da):58,402
Last modified:April 1, 1990 - v1
Checksum:i29AC2B543CBE6B52
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17611 mRNA. Translation: CAA35613.1.
PIRiS08214.
UniGeneiRn.48774.

Genome annotation databases

UCSCiRGD:2099. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17611 mRNA. Translation: CAA35613.1.
PIRiS08214.
UniGeneiRn.48774.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4KJDX-ray2.21A/B21-502[»]
4KJGX-ray2.38A/B21-502[»]
ProteinModelPortaliP15693.
SMRiP15693.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026190.

Chemistry databases

BindingDBiP15693.

Proteomic databases

PaxDbiP15693.
PRIDEiP15693.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:2099. rat.

Organism-specific databases

RGDi2099. Alpi.

Phylogenomic databases

eggNOGiKOG4126. Eukaryota.
COG1785. LUCA.
HOGENOMiHOG000099118.
HOVERGENiHBG007345.
InParanoidiP15693.
PhylomeDBiP15693.

Enzyme and pathway databases

SABIO-RKP15693.

Miscellaneous databases

PROiP15693.

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPPBI1_RAT
AccessioniPrimary (citable) accession number: P15693
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: November 30, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney). Rat has two genes for the intestinal isozyme.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.