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P15693

- PPBI1_RAT

UniProt

P15693 - PPBI1_RAT

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Protein
Intestinal-type alkaline phosphatase 1
Gene
Alpi
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.2 Publications

Cofactori

Binds 1 magnesium ion.1 Publication
Binds 2 zinc ions.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi62 – 621Magnesium
Metal bindingi62 – 621Zinc 1
Active sitei112 – 1121Phosphoserine intermediate1 Publication
Metal bindingi112 – 1121Zinc 1
Metal bindingi175 – 1751Magnesium
Metal bindingi331 – 3311Magnesium
Metal bindingi336 – 3361Zinc 2
Metal bindingi340 – 3401Zinc 2; via tele nitrogen
Metal bindingi377 – 3771Zinc 1
Metal bindingi378 – 3781Zinc 1; via tele nitrogen
Metal bindingi452 – 4521Zinc 2; via tele nitrogen

GO - Molecular functioni

  1. alkaline phosphatase activity Source: UniProtKB
  2. magnesium ion binding Source: UniProtKB
  3. protein binding Source: UniProtKB
  4. protein homodimerization activity Source: UniProtKB
  5. zinc ion binding Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. dephosphorylation Source: UniProtKB
  2. phosphorus metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

SABIO-RKiP15693.

Names & Taxonomyi

Protein namesi
Recommended name:
Intestinal-type alkaline phosphatase 1 (EC:3.1.3.1)
Short name:
IAP-1
Short name:
Intestinal alkaline phosphatase 1
Alternative name(s):
Intestinal alkaline phosphatase I
Short name:
IAP-I
Gene namesi
Name:Alpi
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi2099. Alpi.

Subcellular locationi

Cell membrane; Lipid-anchorGPI-anchor 2 Publications

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. external side of plasma membrane Source: RGD
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 20201 Publication
Add
BLAST
Chaini21 – 511491Intestinal-type alkaline phosphatase 1
PRO_0000024041Add
BLAST
Propeptidei512 – 54029Removed in mature form Reviewed prediction
PRO_0000024042Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi141 ↔ 2031 Publication
Glycosylationi142 – 1421N-linked (GlcNAc...) Reviewed prediction
Glycosylationi301 – 3011N-linked (GlcNAc...)1 Publication
Glycosylationi428 – 4281N-linked (GlcNAc...)1 Publication
Disulfide bondi487 ↔ 4941 Publication
Lipidationi511 – 5111GPI-anchor amidated asparagine Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Proteomic databases

PRIDEiP15693.

Expressioni

Gene expression databases

GenevestigatoriP15693.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026190.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi25 – 273
Helixi29 – 4517
Beta strandi54 – 618
Helixi66 – 7914
Helixi91 – 933
Beta strandi95 – 1017
Beta strandi105 – 1095
Helixi112 – 12110
Beta strandi130 – 1323
Helixi141 – 1444
Helixi152 – 1587
Beta strandi162 – 1709
Helixi174 – 1774
Turni178 – 1803
Helixi191 – 1933
Helixi196 – 2005
Helixi206 – 2127
Beta strandi217 – 2226
Helixi224 – 2274
Helixi241 – 2433
Beta strandi247 – 2493
Helixi253 – 2586
Beta strandi264 – 2674
Helixi270 – 2789
Beta strandi284 – 2885
Beta strandi290 – 2934
Helixi297 – 2993
Turni302 – 3043
Helixi308 – 31912
Beta strandi326 – 3327
Helixi335 – 3384
Helixi344 – 36421
Turni367 – 3693
Beta strandi370 – 3789
Beta strandi379 – 3846
Beta strandi409 – 4168
Helixi431 – 4344
Beta strandi443 – 4464
Beta strandi457 – 4637
Helixi466 – 4683
Beta strandi471 – 4744
Helixi477 – 4859

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4KJDX-ray2.21A/B21-502[»]
4KJGX-ray2.38A/B21-502[»]
ProteinModelPortaliP15693.
SMRiP15693. Positions 21-500.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane

Phylogenomic databases

eggNOGiCOG1785.
HOGENOMiHOG000099118.
HOVERGENiHBG007345.
PhylomeDBiP15693.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15693-1 [UniParc]FASTAAdd to Basket

« Hide

MQGDWVLLLL LGLRIHLSFG VIPVEEENPV FWNQKAKEAL DVAKKLQPIQ    50
TSAKNLILFL GDGMGVPTVT ATRILKGQLG GHLGPETPLA MDHFPFTALS 100
KTYNVDRQVP DSAGTATAYL CGVKANYKTI GVSAAARFNQ CNSTFGNEVF 150
SVMHRAKKAG KSVGVVTTTR VQHASPAGTY AHTVNRDWYS DADMPSSALQ 200
EGCKDIATQL ISNMDIDVIL GGGRKFMFPK GTPDPEYPGD SDQSGVRLDS 250
RNLVEEWLAK YQGTRYVWNR EQLMQASQDP AVTRLMGLFE PTEMKYDVNR 300
NASADPSLAE MTEVAVRLLS RNPQGFYLFV EGGRIDQGHH AGTAYLALTE 350
AVMFDSAIEK ASQLTNEKDT LTLITADHSH VFAFGGYTLR GTSIFGLAPL 400
NAQDGKSYTS ILYGNGPGYV LNSGNRPNVT DAESGDVNYK QQAAVPLSSE 450
THGGEDVAIF ARGPQAHLVH GVQEQNYIAH VMAFAGCLEP YTDCGLAPPA 500
DENRPTTPVQ NSAITMNNVL LSLQLLVSML LLVGTALVVS 540
Length:540
Mass (Da):58,402
Last modified:April 1, 1990 - v1
Checksum:i29AC2B543CBE6B52
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X17611 mRNA. Translation: CAA35613.1.
PIRiS08214.
UniGeneiRn.48774.

Genome annotation databases

UCSCiRGD:2099. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X17611 mRNA. Translation: CAA35613.1 .
PIRi S08214.
UniGenei Rn.48774.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4KJD X-ray 2.21 A/B 21-502 [» ]
4KJG X-ray 2.38 A/B 21-502 [» ]
ProteinModelPortali P15693.
SMRi P15693. Positions 21-500.
ModBasei Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000026190.

Proteomic databases

PRIDEi P15693.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:2099. rat.

Organism-specific databases

RGDi 2099. Alpi.

Phylogenomic databases

eggNOGi COG1785.
HOGENOMi HOG000099118.
HOVERGENi HBG007345.
PhylomeDBi P15693.

Enzyme and pathway databases

SABIO-RKi P15693.

Miscellaneous databases

NextBioi 13948935.
PROi P15693.

Gene expression databases

Genevestigatori P15693.

Family and domain databases

Gene3Di 3.40.720.10. 1 hit.
InterProi IPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view ]
Pfami PF00245. Alk_phosphatase. 1 hit.
[Graphical view ]
PRINTSi PR00113. ALKPHPHTASE.
SMARTi SM00098. alkPPc. 1 hit.
[Graphical view ]
SUPFAMi SSF53649. SSF53649. 1 hit.
PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and expression of a cDNA encoding the membrane-associated rat intestinal alkaline phosphatase."
    Lowe M., Strauss A.W., Alpers R., Seetharam S., Alpers D.H.
    Biochim. Biophys. Acta 1037:170-177(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 21-34 AND 287-300, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION.
  2. "Two rat intestinal alkaline phosphatase isoforms with different carboxyl-terminal peptides are both membrane-bound by a glycan phosphatidylinositol linkage."
    Engle M.J., Mahmood A., Alpers D.H.
    J. Biol. Chem. 270:11935-11940(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: GPI-ANCHOR.
  3. "Crystal structure of rat intestinal alkaline phosphatase - Role of crown domain in mammalian alkaline phosphatases."
    Ghosh K., Mazumder Tagore D., Anumula R., Lakshmaiah B., Kumar P.P., Singaram S., Matan T., Kallipatti S., Selvam S., Krishnamurthy P., Ramarao M.
    J. Struct. Biol. 184:182-192(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.21 ANGSTROMS) OF 21-502 IN COMPLEX WITH MAGNESIUM AND ZINC, GLYCOSYLATION AT ASN-301 AND ASN-428, DISULFIDE BOND, COFACTOR, CATALYTIC ACTIVITY, SUBUNIT, ACTIVE SITE.

Entry informationi

Entry nameiPPBI1_RAT
AccessioniPrimary (citable) accession number: P15693
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: June 11, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney). Rat has two genes for the intestinal isozyme.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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