Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P15693

- PPBI1_RAT

UniProt

P15693 - PPBI1_RAT

Protein

Intestinal-type alkaline phosphatase 1

Gene

Alpi

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (01 Apr 1990)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.2 PublicationsPROSITE-ProRule annotation

    Cofactori

    Binds 1 magnesium ion.1 Publication
    Binds 2 zinc ions.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi62 – 621Magnesium1 Publication
    Metal bindingi62 – 621Zinc 11 Publication
    Active sitei112 – 1121Phosphoserine intermediate1 PublicationPROSITE-ProRule annotation
    Metal bindingi112 – 1121Zinc 11 Publication
    Metal bindingi175 – 1751Magnesium1 Publication
    Metal bindingi331 – 3311Magnesium1 Publication
    Metal bindingi336 – 3361Zinc 21 Publication
    Metal bindingi340 – 3401Zinc 2; via tele nitrogen1 Publication
    Metal bindingi377 – 3771Zinc 11 Publication
    Metal bindingi378 – 3781Zinc 1; via tele nitrogen1 Publication
    Metal bindingi452 – 4521Zinc 2; via tele nitrogen1 Publication

    GO - Molecular functioni

    1. alkaline phosphatase activity Source: UniProtKB
    2. magnesium ion binding Source: UniProtKB
    3. protein binding Source: UniProtKB
    4. protein homodimerization activity Source: UniProtKB
    5. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. dephosphorylation Source: UniProtKB
    2. phosphorus metabolic process Source: RGD

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Zinc

    Enzyme and pathway databases

    SABIO-RKP15693.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Intestinal-type alkaline phosphatase 1 (EC:3.1.3.1)
    Short name:
    IAP-1
    Short name:
    Intestinal alkaline phosphatase 1
    Alternative name(s):
    Intestinal alkaline phosphatase I
    Short name:
    IAP-I
    Gene namesi
    Name:Alpi
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi2099. Alpi.

    Subcellular locationi

    Cell membrane 1 Publication; Lipid-anchorGPI-anchor 1 Publication

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. external side of plasma membrane Source: RGD
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 20201 PublicationAdd
    BLAST
    Chaini21 – 511491Intestinal-type alkaline phosphatase 1PRO_0000024041Add
    BLAST
    Propeptidei512 – 54029Removed in mature formSequence AnalysisPRO_0000024042Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi141 ↔ 2031 Publication
    Glycosylationi142 – 1421N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi301 – 3011N-linked (GlcNAc...)1 Publication
    Glycosylationi428 – 4281N-linked (GlcNAc...)1 Publication
    Disulfide bondi487 ↔ 4941 Publication
    Lipidationi511 – 5111GPI-anchor amidated asparagineSequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

    Proteomic databases

    PRIDEiP15693.

    Expressioni

    Gene expression databases

    GenevestigatoriP15693.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000026190.

    Structurei

    Secondary structure

    1
    540
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi25 – 273
    Helixi29 – 4517
    Beta strandi54 – 618
    Helixi66 – 7914
    Helixi91 – 933
    Beta strandi95 – 1017
    Beta strandi105 – 1095
    Helixi112 – 12110
    Beta strandi130 – 1323
    Helixi141 – 1444
    Helixi152 – 1587
    Beta strandi162 – 1709
    Helixi174 – 1774
    Turni178 – 1803
    Helixi191 – 1933
    Helixi196 – 2005
    Helixi206 – 2127
    Beta strandi217 – 2226
    Helixi224 – 2274
    Helixi241 – 2433
    Beta strandi247 – 2493
    Helixi253 – 2586
    Beta strandi264 – 2674
    Helixi270 – 2789
    Beta strandi284 – 2885
    Beta strandi290 – 2934
    Helixi297 – 2993
    Turni302 – 3043
    Helixi308 – 31912
    Beta strandi326 – 3327
    Helixi335 – 3384
    Helixi344 – 36421
    Turni367 – 3693
    Beta strandi370 – 3789
    Beta strandi379 – 3846
    Beta strandi409 – 4168
    Helixi431 – 4344
    Beta strandi443 – 4464
    Beta strandi457 – 4637
    Helixi466 – 4683
    Beta strandi471 – 4744
    Helixi477 – 4859

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4KJDX-ray2.21A/B21-502[»]
    4KJGX-ray2.38A/B21-502[»]
    ProteinModelPortaliP15693.
    SMRiP15693. Positions 21-500.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the alkaline phosphatase family.Curated

    Keywords - Domaini

    Signal, Transmembrane

    Phylogenomic databases

    eggNOGiCOG1785.
    HOGENOMiHOG000099118.
    HOVERGENiHBG007345.
    PhylomeDBiP15693.

    Family and domain databases

    Gene3Di3.40.720.10. 1 hit.
    InterProiIPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view]
    PfamiPF00245. Alk_phosphatase. 1 hit.
    [Graphical view]
    PRINTSiPR00113. ALKPHPHTASE.
    SMARTiSM00098. alkPPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF53649. SSF53649. 1 hit.
    PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P15693-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQGDWVLLLL LGLRIHLSFG VIPVEEENPV FWNQKAKEAL DVAKKLQPIQ    50
    TSAKNLILFL GDGMGVPTVT ATRILKGQLG GHLGPETPLA MDHFPFTALS 100
    KTYNVDRQVP DSAGTATAYL CGVKANYKTI GVSAAARFNQ CNSTFGNEVF 150
    SVMHRAKKAG KSVGVVTTTR VQHASPAGTY AHTVNRDWYS DADMPSSALQ 200
    EGCKDIATQL ISNMDIDVIL GGGRKFMFPK GTPDPEYPGD SDQSGVRLDS 250
    RNLVEEWLAK YQGTRYVWNR EQLMQASQDP AVTRLMGLFE PTEMKYDVNR 300
    NASADPSLAE MTEVAVRLLS RNPQGFYLFV EGGRIDQGHH AGTAYLALTE 350
    AVMFDSAIEK ASQLTNEKDT LTLITADHSH VFAFGGYTLR GTSIFGLAPL 400
    NAQDGKSYTS ILYGNGPGYV LNSGNRPNVT DAESGDVNYK QQAAVPLSSE 450
    THGGEDVAIF ARGPQAHLVH GVQEQNYIAH VMAFAGCLEP YTDCGLAPPA 500
    DENRPTTPVQ NSAITMNNVL LSLQLLVSML LLVGTALVVS 540
    Length:540
    Mass (Da):58,402
    Last modified:April 1, 1990 - v1
    Checksum:i29AC2B543CBE6B52
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17611 mRNA. Translation: CAA35613.1.
    PIRiS08214.
    UniGeneiRn.48774.

    Genome annotation databases

    UCSCiRGD:2099. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17611 mRNA. Translation: CAA35613.1 .
    PIRi S08214.
    UniGenei Rn.48774.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4KJD X-ray 2.21 A/B 21-502 [» ]
    4KJG X-ray 2.38 A/B 21-502 [» ]
    ProteinModelPortali P15693.
    SMRi P15693. Positions 21-500.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000026190.

    Proteomic databases

    PRIDEi P15693.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:2099. rat.

    Organism-specific databases

    RGDi 2099. Alpi.

    Phylogenomic databases

    eggNOGi COG1785.
    HOGENOMi HOG000099118.
    HOVERGENi HBG007345.
    PhylomeDBi P15693.

    Enzyme and pathway databases

    SABIO-RK P15693.

    Miscellaneous databases

    NextBioi 13948935.
    PROi P15693.

    Gene expression databases

    Genevestigatori P15693.

    Family and domain databases

    Gene3Di 3.40.720.10. 1 hit.
    InterProi IPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view ]
    Pfami PF00245. Alk_phosphatase. 1 hit.
    [Graphical view ]
    PRINTSi PR00113. ALKPHPHTASE.
    SMARTi SM00098. alkPPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53649. SSF53649. 1 hit.
    PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and expression of a cDNA encoding the membrane-associated rat intestinal alkaline phosphatase."
      Lowe M., Strauss A.W., Alpers R., Seetharam S., Alpers D.H.
      Biochim. Biophys. Acta 1037:170-177(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 21-34 AND 287-300, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION.
    2. "Two rat intestinal alkaline phosphatase isoforms with different carboxyl-terminal peptides are both membrane-bound by a glycan phosphatidylinositol linkage."
      Engle M.J., Mahmood A., Alpers D.H.
      J. Biol. Chem. 270:11935-11940(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: GPI-ANCHOR.
    3. "Crystal structure of rat intestinal alkaline phosphatase - Role of crown domain in mammalian alkaline phosphatases."
      Ghosh K., Mazumder Tagore D., Anumula R., Lakshmaiah B., Kumar P.P., Singaram S., Matan T., Kallipatti S., Selvam S., Krishnamurthy P., Ramarao M.
      J. Struct. Biol. 184:182-192(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.21 ANGSTROMS) OF 21-502 IN COMPLEX WITH MAGNESIUM AND ZINC, GLYCOSYLATION AT ASN-301 AND ASN-428, DISULFIDE BOND, COFACTOR, CATALYTIC ACTIVITY, SUBUNIT, ACTIVE SITE.

    Entry informationi

    Entry nameiPPBI1_RAT
    AccessioniPrimary (citable) accession number: P15693
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: April 1, 1990
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney). Rat has two genes for the intestinal isozyme.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3