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P15693

- PPBI1_RAT

UniProt

P15693 - PPBI1_RAT

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Protein

Intestinal-type alkaline phosphatase 1

Gene

Alpi

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.2 PublicationsPROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi62 – 621Magnesium1 Publication
Metal bindingi62 – 621Zinc 11 Publication
Active sitei112 – 1121Phosphoserine intermediate1 PublicationPROSITE-ProRule annotation
Metal bindingi112 – 1121Zinc 11 Publication
Metal bindingi175 – 1751Magnesium1 Publication
Metal bindingi331 – 3311Magnesium1 Publication
Metal bindingi336 – 3361Zinc 21 Publication
Metal bindingi340 – 3401Zinc 2; via tele nitrogen1 Publication
Metal bindingi377 – 3771Zinc 11 Publication
Metal bindingi378 – 3781Zinc 1; via tele nitrogen1 Publication
Metal bindingi452 – 4521Zinc 2; via tele nitrogen1 Publication

GO - Molecular functioni

  1. alkaline phosphatase activity Source: UniProtKB
  2. magnesium ion binding Source: UniProtKB
  3. protein homodimerization activity Source: UniProtKB
  4. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. dephosphorylation Source: UniProtKB
  2. phosphorus metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Intestinal-type alkaline phosphatase 1 (EC:3.1.3.1)
Short name:
IAP-1
Short name:
Intestinal alkaline phosphatase 1
Alternative name(s):
Intestinal alkaline phosphatase I
Short name:
IAP-I
Gene namesi
Name:Alpi
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi2099. Alpi.

Subcellular locationi

Cell membrane 1 Publication; Lipid-anchorGPI-anchor 1 Publication

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. external side of plasma membrane Source: RGD
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 20201 PublicationAdd
BLAST
Chaini21 – 511491Intestinal-type alkaline phosphatase 1PRO_0000024041Add
BLAST
Propeptidei512 – 54029Removed in mature formSequence AnalysisPRO_0000024042Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi141 ↔ 2031 Publication
Glycosylationi142 – 1421N-linked (GlcNAc...)Sequence Analysis
Glycosylationi301 – 3011N-linked (GlcNAc...)1 Publication
Glycosylationi428 – 4281N-linked (GlcNAc...)1 Publication
Disulfide bondi487 ↔ 4941 Publication
Lipidationi511 – 5111GPI-anchor amidated asparagineSequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Proteomic databases

PRIDEiP15693.

Expressioni

Gene expression databases

GenevestigatoriP15693.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026190.

Structurei

Secondary structure

1
540
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi25 – 273Combined sources
Helixi29 – 4517Combined sources
Beta strandi54 – 618Combined sources
Helixi66 – 7914Combined sources
Helixi91 – 933Combined sources
Beta strandi95 – 1017Combined sources
Beta strandi105 – 1095Combined sources
Helixi112 – 12110Combined sources
Beta strandi130 – 1323Combined sources
Helixi141 – 1444Combined sources
Helixi152 – 1587Combined sources
Beta strandi162 – 1709Combined sources
Helixi174 – 1774Combined sources
Turni178 – 1803Combined sources
Helixi191 – 1933Combined sources
Helixi196 – 2005Combined sources
Helixi206 – 2127Combined sources
Beta strandi217 – 2226Combined sources
Helixi224 – 2274Combined sources
Helixi241 – 2433Combined sources
Beta strandi247 – 2493Combined sources
Helixi253 – 2586Combined sources
Beta strandi264 – 2674Combined sources
Helixi270 – 2789Combined sources
Beta strandi284 – 2885Combined sources
Beta strandi290 – 2934Combined sources
Helixi297 – 2993Combined sources
Turni302 – 3043Combined sources
Helixi308 – 31912Combined sources
Beta strandi326 – 3327Combined sources
Helixi335 – 3384Combined sources
Helixi344 – 36421Combined sources
Turni367 – 3693Combined sources
Beta strandi370 – 3789Combined sources
Beta strandi379 – 3846Combined sources
Beta strandi409 – 4168Combined sources
Helixi431 – 4344Combined sources
Beta strandi443 – 4464Combined sources
Beta strandi457 – 4637Combined sources
Helixi466 – 4683Combined sources
Beta strandi471 – 4744Combined sources
Helixi477 – 4859Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4KJDX-ray2.21A/B21-502[»]
4KJGX-ray2.38A/B21-502[»]
ProteinModelPortaliP15693.
SMRiP15693. Positions 21-500.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal, Transmembrane

Phylogenomic databases

eggNOGiCOG1785.
HOGENOMiHOG000099118.
HOVERGENiHBG007345.
InParanoidiP15693.
PhylomeDBiP15693.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15693-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQGDWVLLLL LGLRIHLSFG VIPVEEENPV FWNQKAKEAL DVAKKLQPIQ
60 70 80 90 100
TSAKNLILFL GDGMGVPTVT ATRILKGQLG GHLGPETPLA MDHFPFTALS
110 120 130 140 150
KTYNVDRQVP DSAGTATAYL CGVKANYKTI GVSAAARFNQ CNSTFGNEVF
160 170 180 190 200
SVMHRAKKAG KSVGVVTTTR VQHASPAGTY AHTVNRDWYS DADMPSSALQ
210 220 230 240 250
EGCKDIATQL ISNMDIDVIL GGGRKFMFPK GTPDPEYPGD SDQSGVRLDS
260 270 280 290 300
RNLVEEWLAK YQGTRYVWNR EQLMQASQDP AVTRLMGLFE PTEMKYDVNR
310 320 330 340 350
NASADPSLAE MTEVAVRLLS RNPQGFYLFV EGGRIDQGHH AGTAYLALTE
360 370 380 390 400
AVMFDSAIEK ASQLTNEKDT LTLITADHSH VFAFGGYTLR GTSIFGLAPL
410 420 430 440 450
NAQDGKSYTS ILYGNGPGYV LNSGNRPNVT DAESGDVNYK QQAAVPLSSE
460 470 480 490 500
THGGEDVAIF ARGPQAHLVH GVQEQNYIAH VMAFAGCLEP YTDCGLAPPA
510 520 530 540
DENRPTTPVQ NSAITMNNVL LSLQLLVSML LLVGTALVVS
Length:540
Mass (Da):58,402
Last modified:April 1, 1990 - v1
Checksum:i29AC2B543CBE6B52
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17611 mRNA. Translation: CAA35613.1.
PIRiS08214.
UniGeneiRn.48774.

Genome annotation databases

UCSCiRGD:2099. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17611 mRNA. Translation: CAA35613.1 .
PIRi S08214.
UniGenei Rn.48774.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4KJD X-ray 2.21 A/B 21-502 [» ]
4KJG X-ray 2.38 A/B 21-502 [» ]
ProteinModelPortali P15693.
SMRi P15693. Positions 21-500.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000026190.

Proteomic databases

PRIDEi P15693.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:2099. rat.

Organism-specific databases

RGDi 2099. Alpi.

Phylogenomic databases

eggNOGi COG1785.
HOGENOMi HOG000099118.
HOVERGENi HBG007345.
InParanoidi P15693.
PhylomeDBi P15693.

Miscellaneous databases

NextBioi 13948935.
PROi P15693.

Gene expression databases

Genevestigatori P15693.

Family and domain databases

Gene3Di 3.40.720.10. 1 hit.
InterProi IPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view ]
Pfami PF00245. Alk_phosphatase. 1 hit.
[Graphical view ]
PRINTSi PR00113. ALKPHPHTASE.
SMARTi SM00098. alkPPc. 1 hit.
[Graphical view ]
SUPFAMi SSF53649. SSF53649. 1 hit.
PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and expression of a cDNA encoding the membrane-associated rat intestinal alkaline phosphatase."
    Lowe M., Strauss A.W., Alpers R., Seetharam S., Alpers D.H.
    Biochim. Biophys. Acta 1037:170-177(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 21-34 AND 287-300, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION.
  2. "Two rat intestinal alkaline phosphatase isoforms with different carboxyl-terminal peptides are both membrane-bound by a glycan phosphatidylinositol linkage."
    Engle M.J., Mahmood A., Alpers D.H.
    J. Biol. Chem. 270:11935-11940(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: GPI-ANCHOR.
  3. "Crystal structure of rat intestinal alkaline phosphatase - Role of crown domain in mammalian alkaline phosphatases."
    Ghosh K., Mazumder Tagore D., Anumula R., Lakshmaiah B., Kumar P.P., Singaram S., Matan T., Kallipatti S., Selvam S., Krishnamurthy P., Ramarao M.
    J. Struct. Biol. 184:182-192(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.21 ANGSTROMS) OF 21-502 IN COMPLEX WITH MAGNESIUM AND ZINC, GLYCOSYLATION AT ASN-301 AND ASN-428, DISULFIDE BOND, COFACTOR, CATALYTIC ACTIVITY, SUBUNIT, ACTIVE SITE.

Entry informationi

Entry nameiPPBI1_RAT
AccessioniPrimary (citable) accession number: P15693
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: November 26, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney). Rat has two genes for the intestinal isozyme.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3