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P15693 (PPBI1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Intestinal-type alkaline phosphatase 1
Short name=IAP-1
Short name=Intestinal alkaline phosphatase 1
EC=3.1.3.1
Alternative name(s):
Intestinal alkaline phosphatase I
Short name=IAP-I
Gene names
Name:Alpi
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length540 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactor

Binds 1 magnesium ion By similarity.

Binds 2 zinc ions By similarity.

Subunit structure

Homodimer.

Subcellular location

Cell membrane; Lipid-anchor › GPI-anchor Ref.2.

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney). Rat has two genes for the intestinal isozyme.

Sequence similarities

Belongs to the alkaline phosphatase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Ref.1
Chain21 – 511491Intestinal-type alkaline phosphatase 1
PRO_0000024041
Propeptide512 – 54029Removed in mature form Potential
PRO_0000024042

Sites

Active site1121Phosphoserine intermediate By similarity
Metal binding621Magnesium Potential
Metal binding621Zinc 2 Potential
Metal binding3311Magnesium Potential
Metal binding3361Zinc 1 Potential
Metal binding3401Zinc 1 Potential
Metal binding3771Zinc 2 Potential
Metal binding3781Zinc 2 Potential
Metal binding4521Zinc 1 Potential

Amino acid modifications

Modified residue1121Phosphoserine By similarity
Lipidation5111GPI-anchor amidated asparagine Potential
Glycosylation1421N-linked (GlcNAc...) Potential
Glycosylation3011N-linked (GlcNAc...) Potential
Glycosylation4281N-linked (GlcNAc...) Potential
Disulfide bond141 ↔ 203 By similarity
Disulfide bond487 ↔ 494 By similarity

Sequences

Sequence LengthMass (Da)Tools
P15693 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: 29AC2B543CBE6B52

FASTA54058,402
        10         20         30         40         50         60 
MQGDWVLLLL LGLRIHLSFG VIPVEEENPV FWNQKAKEAL DVAKKLQPIQ TSAKNLILFL 

        70         80         90        100        110        120 
GDGMGVPTVT ATRILKGQLG GHLGPETPLA MDHFPFTALS KTYNVDRQVP DSAGTATAYL 

       130        140        150        160        170        180 
CGVKANYKTI GVSAAARFNQ CNSTFGNEVF SVMHRAKKAG KSVGVVTTTR VQHASPAGTY 

       190        200        210        220        230        240 
AHTVNRDWYS DADMPSSALQ EGCKDIATQL ISNMDIDVIL GGGRKFMFPK GTPDPEYPGD 

       250        260        270        280        290        300 
SDQSGVRLDS RNLVEEWLAK YQGTRYVWNR EQLMQASQDP AVTRLMGLFE PTEMKYDVNR 

       310        320        330        340        350        360 
NASADPSLAE MTEVAVRLLS RNPQGFYLFV EGGRIDQGHH AGTAYLALTE AVMFDSAIEK 

       370        380        390        400        410        420 
ASQLTNEKDT LTLITADHSH VFAFGGYTLR GTSIFGLAPL NAQDGKSYTS ILYGNGPGYV 

       430        440        450        460        470        480 
LNSGNRPNVT DAESGDVNYK QQAAVPLSSE THGGEDVAIF ARGPQAHLVH GVQEQNYIAH 

       490        500        510        520        530        540 
VMAFAGCLEP YTDCGLAPPA DENRPTTPVQ NSAITMNNVL LSLQLLVSML LLVGTALVVS

« Hide

References

[1]"Molecular cloning and expression of a cDNA encoding the membrane-associated rat intestinal alkaline phosphatase."
Lowe M., Strauss A.W., Alpers R., Seetharam S., Alpers D.H.
Biochim. Biophys. Acta 1037:170-177(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 21-34 AND 287-300.
[2]"Two rat intestinal alkaline phosphatase isoforms with different carboxyl-terminal peptides are both membrane-bound by a glycan phosphatidylinositol linkage."
Engle M.J., Mahmood A., Alpers D.H.
J. Biol. Chem. 270:11935-11940(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: GPI-ANCHOR.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X17611 mRNA. Translation: CAA35613.1.
IPIIPI00327516.
PIRS08214.
UniGeneRn.48774.

3D structure databases

ProteinModelPortalP15693.
SMRP15693. Positions 21-500.
ModBaseSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000026190.

Proteomic databases

PRIDEP15693.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:2099. rat.

Organism-specific databases

RGD2099. Alpi.

Phylogenomic databases

eggNOGCOG1785.
HOGENOMHOG000099118.
HOVERGENHBG007345.
OrthoDBEOG4GB765.

Enzyme and pathway databases

SABIO-RKP15693.

Gene expression databases

GenevestigatorP15693.
GermOnlineENSRNOG00000030020. Rattus norvegicus.

Family and domain databases

Gene3D3.40.720.10. 1 hit.
InterProIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSPR00113. ALKPHPHTASE.
SMARTSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMSSF53649. Alkaline_phosphatase_core. 1 hit.
PROSITEPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio13948935.

Entry information

Entry namePPBI1_RAT
AccessionPrimary (citable) accession number: P15693
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: April 3, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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