Reviewed,
UniProtKB/Swiss-Prot P15690 (NDUS1_BOVIN)
Last modified
June 16, 2009.
Version 87.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial EC=1.6.5.3 EC=1.6.99.3 Alternative name(s): Complex I-75kD Short name=CI-75kD | ||
| Gene names |
| ||
| Organism | Bos taurus (Bovine) | ||
| Taxonomic identifier | 9913 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Protein attributes
| Sequence length | 727 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. |
| Catalytic activity | NADH + ubiquinone = NAD+ + ubiquinol. NADH + acceptor = NAD+ + reduced acceptor. |
| Cofactor | Binds 1 2Fe-2S cluster per subunit By similarity. Binds 2 4Fe-4S clusters per subunit By similarity. |
| Subunit structure | Complex I is composed of 45 different subunits. |
| Subcellular location | |
| Sequence similarities | Belongs to the complex I 75 kDa subunit family. Contains 1 2Fe-2S ferredoxin-type domain. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 23 | 23 | Mitochondrion | ||||||
| Chain | 24 – 727 | 704 | NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial Ref.1 | PRO_0000019967 | |||||
Regions | |||||||||
| Domain | 30 – 108 | 79 | 2Fe-2S ferredoxin-type | ||||||
Sites | |||||||||
| Metal binding | 64 | 1 | Iron-sulfur 1 (2Fe-2S) By similarity | ||||||
| Metal binding | 75 | 1 | Iron-sulfur 1 (2Fe-2S) By similarity | ||||||
| Metal binding | 78 | 1 | Iron-sulfur 1 (2Fe-2S) By similarity | ||||||
| Metal binding | 92 | 1 | Iron-sulfur 1 (2Fe-2S) By similarity | ||||||
| Metal binding | 124 | 1 | Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity | ||||||
| Metal binding | 128 | 1 | Iron-sulfur 2 (4Fe-4S) By similarity | ||||||
| Metal binding | 131 | 1 | Iron-sulfur 2 (4Fe-4S) By similarity | ||||||
| Metal binding | 137 | 1 | Iron-sulfur 2 (4Fe-4S) By similarity | ||||||
| Metal binding | 176 | 1 | Iron-sulfur 3 (4Fe-4S) By similarity | ||||||
| Metal binding | 179 | 1 | Iron-sulfur 3 (4Fe-4S) By similarity | ||||||
| Metal binding | 182 | 1 | Iron-sulfur 3 (4Fe-4S) By similarity | ||||||
| Metal binding | 226 | 1 | Iron-sulfur 3 (4Fe-4S) By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 84 | 1 | N6-acetyllysine By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Mitochondrial NADH:ubiquinone reductase: complementary DNA sequence of the import precursor of the bovine 75-kDa subunit." Runswick M.J., Gennis R.B., Fearnley I.M., Walker J.E. Biochemistry 28:9452-9459(1989) [PubMed: 2514801] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. |
| [2] | NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal pons. |
Cross-references
Sequence databases | |
|---|---|
| J02877 mRNA. Translation: AAA30662.1. BC120069 mRNA. Translation: AAI20070.1. | |
| IPI | IPI00708027. |
| PIR | A33552. |
| RefSeq | NP_777245.1. |
| UniGene | Bt.4777 |
3D structure databases | |
| ModBase | Search... |
Protein family/group databases | |
| TCDB | 3.D.1.6.1. proton-translocating NADH dehydrogenase (NDH) family. |
Genome annotation databases | |
| Ensembl | ENSBTAG00000021976. Bos taurus. [Contig view] |
| GeneID | 288380. |
| KEGG | bta:288380. |
Phylogenomic databases | |
| HOVERGEN | P15690. |
| OMA | P15690. RMSSGVT. |
Enzyme and pathway databases | |
| BRENDA | 1.6.5.3. 251. 1.6.99.3. 251. |
Family and domain databases | |
| InterPro | IPR006058. 2Fe2S_fd_BS. IPR001041. Ferredoxin. IPR006656. Mopterin_OxRdtase. IPR000283. NADH_UbQ_OxRdtase_75KDa_su_CS. IPR010228. NADH_UbQ_OxRdtase_Gsu. IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd. IPR015405. NuoG_C. [Graphical view] |
| Pfam | PF09326. DUF1982. 1 hit. PF00111. Fer2. 1 hit. PF00384. Molybdopterin. 1 hit. PF10588. NADH-G_4Fe-4S_3. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR01973. NuoG. 1 hit. |
| PROSITE | PS00197. 2FE2S_FER_1. False negative. PS51085. 2FE2S_FER_2. 1 hit. PS00641. COMPLEX1_75K_1. 1 hit. PS00642. COMPLEX1_75K_2. 1 hit. PS00643. COMPLEX1_75K_3. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | NDUS1_BOVIN | ||||||||
| Accession | Primary (citable) accession number: P15690 Secondary accession number(s): Q0VCP7 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||

Clusters with


