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P15690

- NDUS1_BOVIN

UniProt

P15690 - NDUS1_BOVIN

Protein

NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

Gene

NDUFS1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 1 (01 Apr 1990)
      Previous versions | rss
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    Functioni

    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized.

    Catalytic activityi

    NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
    NADH + acceptor = NAD+ + reduced acceptor.

    Cofactori

    Binds 1 2Fe-2S cluster per subunit.By similarity
    Binds 2 4Fe-4S clusters per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi64 – 641Iron-sulfur 1 (2Fe-2S)By similarity
    Metal bindingi75 – 751Iron-sulfur 1 (2Fe-2S)By similarity
    Metal bindingi78 – 781Iron-sulfur 1 (2Fe-2S)By similarity
    Metal bindingi92 – 921Iron-sulfur 1 (2Fe-2S)By similarity
    Metal bindingi124 – 1241Iron-sulfur 2 (4Fe-4S); via pros nitrogenBy similarity
    Metal bindingi128 – 1281Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi131 – 1311Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi137 – 1371Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi176 – 1761Iron-sulfur 3 (4Fe-4S)By similarity
    Metal bindingi179 – 1791Iron-sulfur 3 (4Fe-4S)By similarity
    Metal bindingi182 – 1821Iron-sulfur 3 (4Fe-4S)By similarity
    Metal bindingi226 – 2261Iron-sulfur 3 (4Fe-4S)By similarity

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. electron carrier activity Source: InterPro
    4. metal ion binding Source: UniProtKB-KW
    5. NADH dehydrogenase (ubiquinone) activity Source: RefGenome

    GO - Biological processi

    1. apoptotic mitochondrial changes Source: UniProtKB
    2. ATP metabolic process Source: UniProtKB
    3. ATP synthesis coupled electron transport Source: InterPro
    4. cellular respiration Source: UniProtKB
    5. reactive oxygen species metabolic process Source: UniProtKB
    6. regulation of mitochondrial membrane potential Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

    Enzyme and pathway databases

    ReactomeiREACT_223064. Respiratory electron transport.

    Protein family/group databases

    TCDBi3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
    Alternative name(s):
    Complex I-75kD
    Short name:
    CI-75kD
    Gene namesi
    Name:NDUFS1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 2

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial intermembrane space Source: UniProtKB
    2. mitochondrial respiratory chain complex I Source: UniProtKB
    3. mitochondrion Source: AgBase

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2323MitochondrionAdd
    BLAST
    Chaini24 – 727704NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrialPRO_0000019967Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei84 – 841N6-acetyllysineBy similarity
    Modified residuei499 – 4991N6-acetyllysineBy similarity
    Modified residuei709 – 7091N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP15690.
    PRIDEiP15690.

    Interactioni

    Subunit structurei

    Complex I is composed of 45 different subunits.

    Protein-protein interaction databases

    IntActiP15690. 6 interactions.
    STRINGi9913.ENSBTAP00000029301.

    Structurei

    3D structure databases

    ProteinModelPortaliP15690.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini30 – 108792Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd
    BLAST
    Domaini245 – 301574Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the complex I 75 kDa subunit family.Curated
    Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1034.
    GeneTreeiENSGT00390000018768.
    HOGENOMiHOG000031442.
    HOVERGENiHBG003482.
    InParanoidiP15690.
    KOiK03934.
    OMAiFQGNDVA.
    OrthoDBiEOG783MTP.
    TreeFamiTF105756.

    Family and domain databases

    Gene3Di3.10.20.30. 1 hit.
    InterProiIPR001041. 2Fe-2S_ferredoxin-type.
    IPR012675. Beta-grasp_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
    IPR010228. NADH_UbQ_OxRdtase_Gsu.
    IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
    IPR015405. NuoG_C.
    [Graphical view]
    PfamiPF09326. DUF1982. 1 hit.
    PF00111. Fer2. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF10588. NADH-G_4Fe-4S_3. 1 hit.
    [Graphical view]
    SMARTiSM00929. NADH-G_4Fe-4S_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF54292. SSF54292. 1 hit.
    TIGRFAMsiTIGR01973. NuoG. 1 hit.
    PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
    PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00641. COMPLEX1_75K_1. 1 hit.
    PS00642. COMPLEX1_75K_2. 1 hit.
    PS00643. COMPLEX1_75K_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P15690-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLRIPVRKAL VGLSKSSKGC VRTTATAASN LIEVFVDGQS VMVEPGTTVL    50
    QACEKVGMQI PRFCYHERLS VAGNCRMCLV EIEKAPKVVA ACAMPVMKGW 100
    NILTNSEKTK KAREGVMEFL LANHPLDCPI CDQGGECDLQ DQSMMFGSDR 150
    SRFLEGKRAV EDKNIGPLVK TIMTRCIQCT RCIRFASEIA GVDDLGTTGR 200
    GNDMQVGTYI EKMFMSELSG NIIDICPVGA LTSKPYAFTA RPWETRKTES 250
    IDVMDAVGSN IVVSTRTGEV MRILPRMHED INEEWISDKT RFAYDGLKRQ 300
    RLTEPMVRNE KGLLTHTTWE DALSRVAGML QSFQGNDVAA IAGGLVDAEA 350
    LIALKDLLNR VDSDTLCTEE VFPTAGAGTD LRSNYLLNTT IAGVEEADVV 400
    LLVGTNPRFE APLFNARIRK SWLHNDLKVA LIGSPVDLTY RYDHLGDSPK 450
    ILQDIASGSH PFSQVLQEAK KPMVILGSSA LQRNDGAAIL AAVSNIAQKI 500
    RTSSGVTGDW KVMNILHRIA SQVAALDLGY KPGVEAIQKN PPKMLFLLGA 550
    DGGCITRQDL PKDCFIVYQG HHGDVGAPIA DVILPGAAYT EKSATYVNTE 600
    GRAQQTKVAV TPPGLAREDW KIIRALSEIA GMTLPYDTLD QVRNRLEEVS 650
    PNLVRYDDVE GANYFQQASE LSKLVNQQLL ADPLVPPQLT IKDFYMTDSI 700
    SRASQTMAKC VKAVTEGAHA VEEPSIC 727
    Length:727
    Mass (Da):79,442
    Last modified:April 1, 1990 - v1
    Checksum:iBEAD8AB5D76FC22D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02877 mRNA. Translation: AAA30662.1.
    BC120069 mRNA. Translation: AAI20070.1.
    PIRiA33552.
    RefSeqiNP_777245.1. NM_174820.4.
    XP_005202770.1. XM_005202713.1.
    XP_005202771.1. XM_005202714.1.
    UniGeneiBt.4777.

    Genome annotation databases

    EnsembliENSBTAT00000029301; ENSBTAP00000029301; ENSBTAG00000021976.
    GeneIDi288380.
    KEGGibta:288380.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02877 mRNA. Translation: AAA30662.1 .
    BC120069 mRNA. Translation: AAI20070.1 .
    PIRi A33552.
    RefSeqi NP_777245.1. NM_174820.4.
    XP_005202770.1. XM_005202713.1.
    XP_005202771.1. XM_005202714.1.
    UniGenei Bt.4777.

    3D structure databases

    ProteinModelPortali P15690.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P15690. 6 interactions.
    STRINGi 9913.ENSBTAP00000029301.

    Protein family/group databases

    TCDBi 3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Proteomic databases

    PaxDbi P15690.
    PRIDEi P15690.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000029301 ; ENSBTAP00000029301 ; ENSBTAG00000021976 .
    GeneIDi 288380.
    KEGGi bta:288380.

    Organism-specific databases

    CTDi 4719.

    Phylogenomic databases

    eggNOGi COG1034.
    GeneTreei ENSGT00390000018768.
    HOGENOMi HOG000031442.
    HOVERGENi HBG003482.
    InParanoidi P15690.
    KOi K03934.
    OMAi FQGNDVA.
    OrthoDBi EOG783MTP.
    TreeFami TF105756.

    Enzyme and pathway databases

    Reactomei REACT_223064. Respiratory electron transport.

    Miscellaneous databases

    NextBioi 20806551.

    Family and domain databases

    Gene3Di 3.10.20.30. 1 hit.
    InterProi IPR001041. 2Fe-2S_ferredoxin-type.
    IPR012675. Beta-grasp_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
    IPR010228. NADH_UbQ_OxRdtase_Gsu.
    IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
    IPR015405. NuoG_C.
    [Graphical view ]
    Pfami PF09326. DUF1982. 1 hit.
    PF00111. Fer2. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF10588. NADH-G_4Fe-4S_3. 1 hit.
    [Graphical view ]
    SMARTi SM00929. NADH-G_4Fe-4S_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54292. SSF54292. 1 hit.
    TIGRFAMsi TIGR01973. NuoG. 1 hit.
    PROSITEi PS51085. 2FE2S_FER_2. 1 hit.
    PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00641. COMPLEX1_75K_1. 1 hit.
    PS00642. COMPLEX1_75K_2. 1 hit.
    PS00643. COMPLEX1_75K_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mitochondrial NADH:ubiquinone reductase: complementary DNA sequence of the import precursor of the bovine 75-kDa subunit."
      Runswick M.J., Gennis R.B., Fearnley I.M., Walker J.E.
      Biochemistry 28:9452-9459(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Fetal pons.

    Entry informationi

    Entry nameiNDUS1_BOVIN
    AccessioniPrimary (citable) accession number: P15690
    Secondary accession number(s): Q0VCP7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: April 1, 1990
    Last modified: October 1, 2014
    This is version 131 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3