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P15687 (NSP1_ROTS1) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Non-structural protein 1

Short name=NSP1
Alternative name(s):
NCVP2
Non-structural RNA-binding protein 53
Short name=NS53
OrganismRotavirus A (strain SA11-Both G3-P5B[2]-I2-R2-C5-M5-A5-N2-T5-E2-H5) (RV-A) (Simian Agent 11 (strain Both)) [Complete proteome]
Taxonomic identifier37137 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostMacaca mulatta (Rhesus macaque) [TaxID: 9544]

Protein attributes

Sequence length495 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Seems to induce the proteasome-dependent degradation of IRF3, IRF5 and IRF7, thereby antagonizing the cellular interferon response and establishment of the antiviral state. Binds and targets IRF3 early post-infection and suppresses IRF3 nuclear translocation By similarity.

Subunit structure

Interacts (via C-terminus) with host IRF3; this interaction leads to IRF3 degradation. Interacts with host IRF7; this interaction leads to IRF7 degradation By similarity.

Subcellular location

Host cytoplasmhost cytoskeleton By similarity.

Domain

The zinc-finger domain is important, but not sufficient for binding and degrading IRF3. It is sometimes described as a RING zinc-finger, but it is atypical and it is unclear whether it is related with ubiquitin ligase activity By similarity.

Sequence similarities

Belongs to the rotavirus A NSP1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 495495Non-structural protein 1
PRO_0000149558

Regions

Zinc finger42 – 7938 Potential
Region1 – 8181RNA-binding By similarity
Region82 – 17796Important for cytoskeleton localization By similarity
Region316 – 495180Interaction with IRF3 By similarity

Sequences

Sequence LengthMass (Da)Tools
P15687 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: D988A936CD1B534B

FASTA49558,484
        10         20         30         40         50         60 
MATFKDACFH YRRLTALNRR LCNIGANSIC MPVPDEKIKG WCLECCQIAD LTHCYGCSLP 

        70         80         90        100        110        120 
HVCKWCVQNR RCFLDNEPHL LKLRTVKHPI TKDKLQCIID LYNIIFPIND KVIRKFERMI 

       130        140        150        160        170        180 
KQRKCRNQYK IEWYNHLLLP ITLNAAAFKF DENNLYYVFG LYEKSVSDIY APYRIVNFIN 

       190        200        210        220        230        240 
EFDKLLLDDI NFTRMSNLPI ELRTIMQEYF QLSRLPSSKL KQIYFSDFTK ETVIFNTYTK 

       250        260        270        280        290        300 
TPGRSIYRNV TEFNWRDELE LYSDLKNDKN KLIAAMMTSK YTRFYAHDNN FGRLKMTIFE 

       310        320        330        340        350        360 
LGHHCQPNYV ASNHPGNASD IQYCKWCNIK YFLSKIDWRI RDMYNLLMEF IKDCYKSNVN 

       370        380        390        400        410        420 
VGHCSSVENI YPLIKRLIWS LFTNHMDQTI EEVFNHMSPV SVEGTNVIML ILGLNISLYN 

       430        440        450        460        470        480 
EIKRTLNVDS IPMVLNLNEF SSIVKSISSK WYNVDELDKL PMSIKSTEEL IEMKNSGTLT 

       490 
EEFELLISNS EDDNE 

« Hide

References

[1]"Conservation of a potential metal binding motif despite extensive sequence diversity in the rotavirus nonstructural protein NS53."
Mitchell D.B., Both G.W.
Virology 174:618-621(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X14914 Genomic RNA. Translation: CAA33039.1.
PIRMNXRSA. S08215.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR002148. Rotavirus_NSP1.
[Graphical view]
PfamPF00981. Rota_NS53. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNSP1_ROTS1
AccessionPrimary (citable) accession number: P15687
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: February 19, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families