P15686 (HUP1_PARKE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
H(+)/hexose cotransporter 1

Gene names

Name:

HUP1

Organism

Parachlorella kessleri (Green alga) (Chlorella kessleri)

Taxonomic identifier

3074 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Chlorophyta › Trebouxiophyceae › Chlorellales › Chlorellales incertae sedis › Parachlorella

Protein attributes

Sequence length	534 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Active uptake of hexoses.
Subcellular location	Membrane; Multi-pass membrane protein.
Induction	By glucose.
Sequence similarities	Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. [View classification]

Ontologies

Keywords
Biological process	Sugar transport Symport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Gene Ontology (GO)
Biological process	carbohydrate transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	substrate-specific transmembrane transporter activity Inferred from electronic annotation. Source: InterPro symporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 534	534	H(+)/hexose cotransporter 1		PRO_0000050428
Regions
	Topological domain	1 – 21	21	Cytoplasmic Potential
	Transmembrane	22 – 42	21	Helical; Name=1; Potential
	Topological domain	43 – 87	45	Extracellular Potential
	Transmembrane	88 – 108	21	Helical; Name=2; Potential
	Topological domain	109 – 114	6	Cytoplasmic Potential
	Transmembrane	115 – 135	21	Helical; Name=3; Potential
	Topological domain	136 – 144	9	Extracellular Potential
	Transmembrane	145 – 165	21	Helical; Name=4; Potential
	Topological domain	166 – 173	8	Cytoplasmic Potential
	Transmembrane	174 – 194	21	Helical; Name=5; Potential
	Topological domain	195 – 204	10	Extracellular Potential
	Transmembrane	205 – 225	21	Helical; Name=6; Potential
	Topological domain	226 – 299	74	Cytoplasmic Potential
	Transmembrane	300 – 322	23	Helical; Name=7; Potential
	Topological domain	323 – 328	6	Extracellular Potential
	Transmembrane	329 – 349	21	Helical; Name=8; Potential
	Topological domain	350 – 352	3	Cytoplasmic Potential
	Transmembrane	353 – 373	21	Helical; Name=9; Potential
	Topological domain	374 – 387	14	Extracellular Potential
	Transmembrane	388 – 408	21	Helical; Name=10; Potential
	Topological domain	409 – 433	25	Cytoplasmic Potential
	Transmembrane	434 – 454	21	Helical; Name=11; Potential
	Topological domain	455 – 456	2	Extracellular Potential
	Transmembrane	457 – 477	21	Helical; Name=12; Potential
	Topological domain	478 – 534	57	Cytoplasmic Potential
Experimental info
	Sequence conflict	60	1	Missing in CAA68813. Ref.1
	Sequence conflict	209	1	P → L in CAA68813. Ref.1
	Sequence conflict	248	1	C → R in CAA68813. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P15686 [UniParc]. Last modified July 15, 1998. Version 2. Checksum: 3B238E8710AA2237	FASTA	534	57,523
	10 20 30 40 50 60 MAGGGVVVVS GRGLSTGDYR GGLTVYVVMV AFMAACGGLL LGYDNGVTGG VVSLEAFEKK 70 80 90 100 110 120 FFPDVWAKKQ EVHEDSPYCT YDNAKLQLFV SSLFLAGLVS CLFASWITRN WGRKVTMGIG 130 140 150 160 170 180 GAFFVAGGLV NAFAQDMAML IVGRVLLGFG VGLGSQVVPQ YLSEVAPFSH RGMLNIGYQL 190 200 210 220 230 240 FVTIGILIAG LVNYAVRDWE NGWRLSLGPA AAPGAILFLG SLVLPESPNF LVEKGKTEKG 250 260 270 280 290 300 REVLQKLCGT SEVDAEFADI VAAVEIARPI TMRQSWASLF TRRYMPQLLT SFVIQFFQQF 310 320 330 340 350 360 TGINAIIFYV PVLFSSLGSA NSAALLNTVV VGAVNVGSTL IAVMFSDKFG RRFLLIEGGI 370 380 390 400 410 420 QCCLAMLTTG VVLAIEFAKY GTDPLPKAVA SGILAVICIF ISGFAWSWGP MGWLIPSEIF 430 440 450 460 470 480 TLETRPAGTA VAVVGNFLFS FVIGQAFVSM LCAMEYGVFL FFAGWLVIMV LCAIFLLPET 490 500 510 520 530 KGVPIERVQA LYARHWFWNR VMGPAAAEVI AEDEKRVAAA SAIIKEEELS KAMK

References

[1]	"The hexose carrier from Chlorella. cDNA cloning of a eucaryotic H+-cotransporter." Sauer N., Tanner W. FEBS Lett. 259:43-46(1989) [PubMed: 2599110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The Chlorella H+/hexose cotransporter gene." Wolf K., Tanner W., Sauer N. Curr. Genet. 19:215-219(1991) [PubMed: 1868571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y07520 mRNA. Translation: CAA68813.1. X55349 Genomic DNA. Translation: CAA39036.1.
PIR	S14144.
3D structure databases
ProteinModelPortal	P15686.
ModBase	Search...
Protein family/group databases
TCDB	2.A.1.1.14. major facilitator superfamily (MFS).
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR020846. MFS_dom. IPR016196. MFS_dom_general_subst_transpt. IPR005828. Sub_transporter. IPR003663. Sugar/inositol_transpt. IPR005829. Sugar_transporter_CS. [Graphical view]
Pfam	PF00083. Sugar_tr. 1 hit. [Graphical view]
PRINTS	PR00171. SUGRTRNSPORT.
SUPFAM	SSF103473. MFS_gen_substrate_transporter. 1 hit.
TIGRFAMs	TIGR00879. SP. 1 hit.
PROSITE	PS50850. MFS. 1 hit. PS00216. SUGAR_TRANSPORT_1. 1 hit. PS00217. SUGAR_TRANSPORT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HUP1_PARKE

Accession

Primary (citable) accession number: P15686
Secondary accession number(s): Q39523

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	July 15, 1998
Last modified:	May 31, 2011
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program