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P15682

- NCAP_I33A0

UniProt

P15682 - NCAP_I33A0

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Protein

Nucleoprotein

Gene
NP
Organism
Influenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1))
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus By similarity.1 Publication

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
  2. structural molecule activity Source: InterPro

GO - Biological processi

  1. viral entry into host cell Source: UniProtKB-KW
  2. viral penetration into host nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

Host-virus interaction, Viral penetration into host nucleus, Virus entry into host cell

Keywords - Ligandi

RNA-binding, Viral nucleoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoprotein
Alternative name(s):
Nucleocapsid protein
Short name:
Protein N
Gene namesi
Name:NP
OrganismiInfluenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1))
Taxonomic identifieri381518 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000000834: Genome

Subcellular locationi

Virion Reviewed prediction. Host nucleus

GO - Cellular componenti

  1. helical viral capsid Source: UniProtKB-KW
  2. host cell nucleus Source: UniProtKB-SubCell
  3. ribonucleoprotein complex Source: UniProtKB-KW
  4. viral nucleocapsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Helical capsid protein, Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 498498NucleoproteinPRO_0000079113Add
BLAST

Post-translational modificationi

Late in virus-infected cells, may be cleaved from a 56-kDa protein to a 53-kDa protein by a cellular caspase. This cleavage might be a marker for the onset of apoptosis in infected cells or have a specific function in virus host interaction.1 Publication

Interactioni

Subunit structurei

Homomultimerizes to form the nucleocapsid. May bind human exportin-1. Binds to viral genomic RNA. Protein-RNA contacts are mediated by a combination of electrostatic interactions between positively charged residues and the phosphate backbone and planar interactions between aromatic side chains and bases By similarity.

Structurei

Secondary structure

1
498
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi22 – 4726
Helixi51 – 544
Helixi57 – 7216
Beta strandi75 – 784
Beta strandi95 – 1006
Beta strandi103 – 1086
Helixi113 – 12311
Turni124 – 1263
Helixi130 – 14718
Helixi151 – 1555
Turni156 – 1583
Helixi161 – 1666
Turni168 – 1714
Turni178 – 1803
Helixi181 – 1833
Helixi186 – 19914
Beta strandi200 – 2023
Beta strandi204 – 2107
Helixi211 – 22919
Helixi233 – 24311
Helixi250 – 26314
Helixi278 – 2869
Helixi291 – 2944
Helixi301 – 3099
Beta strandi313 – 3164
Helixi322 – 33312
Helixi341 – 3488
Helixi355 – 3573
Beta strandi371 – 3733
Beta strandi375 – 3784
Beta strandi384 – 3907
Helixi422 – 4287
Beta strandi433 – 4353
Helixi439 – 44911
Beta strandi460 – 4656
Beta strandi470 – 4723
Turni487 – 4904

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ZDPX-ray2.69A/B/C1-498[»]
4BBLelectron microscopy18.00A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X8-498[»]
4IRYX-ray2.80A/B1-401[»]
A/B430-498[»]
ProteinModelPortaliP15682.
SMRiP15682. Positions 22-496.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1 – 1818Unconventional nuclear localization signal By similarityAdd
BLAST
Motifi198 – 21619Bipartite nuclear localization signal By similarityAdd
BLAST

Sequence similaritiesi

Family and domain databases

InterProiIPR002141. Flu_NP.
[Graphical view]
PfamiPF00506. Flu_NP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P15682-1 [UniParc]FASTAAdd to Basket

« Hide

MATKGTKRSY EQMETDGERQ NATEIRASVG KMIGGIGRFY IQMCTELKLS    50
DYEGRLIQNS LTIERMVLSA FDERRNKYLE EHPSAGKDPK KTGGPIYRRV 100
DGKWMRELIL YDKEEIRRIW RQANNGDDAT AGLTHMMIWH SNLNDATYQR 150
TRALVRTGMD PRMCSLMQGS TLPRRSGAAG AAVKGVGTMV MELIRMIKRG 200
INDRNFWRGE NGRRTRIAYE RMCNILKGKF QTAAQRAMVD QVRESRNPGN 250
AEFEDLIFLA RSALILRGSV AHKSCLPACV YGPAVASGYD FEREGYSLVG 300
IDPFRLLQNS QVYSLIRPNE NPAHKSQLVW MACHSAAFED LRVSSFIRGT 350
KVVPRGKLST RGVQIASNEN METMESSTLE LRSRYWAIRT RSGGNTNQQR 400
ASSGQISIQP TFSVQRNLPF DRPTIMAAFT GNTEGRTSDM RTEIIRLMES 450
ARPEDVSFQG RGVFELSDEK AASPIVPSFD MSNEGSYFFG DNAEEYDN 498
Length:498
Mass (Da):56,244
Last modified:April 1, 1990 - v1
Checksum:i27AA1703050AA877
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M30746 Genomic RNA. Translation: AAA43452.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M30746 Genomic RNA. Translation: AAA43452.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3ZDP X-ray 2.69 A/B/C 1-498 [» ]
4BBL electron microscopy 18.00 A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X 8-498 [» ]
4IRY X-ray 2.80 A/B 1-401 [» ]
A/B 430-498 [» ]
ProteinModelPortali P15682.
SMRi P15682. Positions 22-496.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR002141. Flu_NP.
[Graphical view ]
Pfami PF00506. Flu_NP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Evolution of the nucleoprotein gene of influenza A virus."
    Gorman O.T., Bean W.J., Kawaoka Y., Webster R.G.
    J. Virol. 64:1487-1497(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Caspase-dependent N-terminal cleavage of influenza virus nucleocapsid protein in infected cells."
    Zhirnov O.P., Konakova T.E., Garten W., Klenk H.
    J. Virol. 73:10158-10163(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CLEAVAGE.
  3. "An unconventional NLS is critical for the nuclear import of the influenza A virus nucleoprotein and ribonucleoprotein."
    Cros J.F., Garcia-Sastre A., Palese P.
    Traffic 6:205-213(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiNCAP_I33A0
AccessioniPrimary (citable) accession number: P15682
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: July 9, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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