SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P15659

- PA_I33A0

UniProt

P15659 - PA_I33A0

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Polymerase acidic protein
Gene
PA
Organism
Influenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1))
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Implicated in endonuclease cleavage of capped RNA primers. Displays an elongation factor activity in viral RNA synthesis. Dispensable for viral transcription, but not replication.

GO - Molecular functioni

  1. RNA binding Source: InterPro
  2. RNA-directed RNA polymerase activity Source: InterPro
  3. protein binding Source: IntAct

GO - Biological processi

  1. suppression by virus of host RNA polymerase II activity Source: UniProtKB-KW
  2. transcription, DNA-templated Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host RNA polymerase II by virus

Names & Taxonomyi

Protein namesi
Recommended name:
Polymerase acidic protein
Alternative name(s):
RNA-directed RNA polymerase subunit P2
Gene namesi
Name:PA
OrganismiInfluenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1))
Taxonomic identifieri381518 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000000834: Genome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi226 – 2261L → P: Confers temperature sensitivity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 716716Polymerase acidic protein
PRO_0000078801Add
BLAST

Post-translational modificationi

Phosphorylated on serines and threonines by host kinases, including human casein kinase II.

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA.

Binary interactionsi

WithEntry#Exp.IntActNotes
USP11P517842EBI-8431752,EBI-306876From a different organism.

Protein-protein interaction databases

IntActiP15659. 2 interactions.
MINTiMINT-8081657.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi273 – 2753
Beta strandi290 – 2945
Turni298 – 3014
Helixi303 – 31210
Beta strandi317 – 3248
Helixi331 – 35020
Beta strandi351 – 3533
Beta strandi356 – 3605
Helixi364 – 3696
Helixi406 – 41510
Beta strandi419 – 4213
Helixi437 – 45014
Helixi454 – 47522
Turni476 – 4783
Beta strandi479 – 49012
Beta strandi496 – 50611
Beta strandi517 – 52610
Helixi534 – 5374
Beta strandi541 – 55010
Beta strandi555 – 57117
Helixi572 – 5798
Helixi580 – 5823
Helixi583 – 60321
Helixi608 – 6125
Helixi633 – 64917
Helixi653 – 67523
Helixi686 – 6916
Helixi698 – 71215

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4IUJX-ray1.90A254-716[»]
ProteinModelPortaliP15659.
SMRiP15659. Positions 257-716.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR001009. RNA-dir_pol_influenzavirus.
[Graphical view]
PfamiPF00603. Flu_PA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by ribosomal frameshifting. Align

Isoform PA (identifier: P15659-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MEDFVRQCFN PMIVELAEKA MKEYGEDLKI ETNKFAAICT HLEVCFMYSD    50
FHFIDEQGES IVVELGDPNA LLKHRFEIIE GRDRTIAWTV INSICNTTGA 100
EKPKFLPDLY DYKKNRFIEI GVTRREVHIY YLEKANKIKS EKTHIHIFSF 150
TGEEMATKAD YTLDEESRAR IKTRLFTIRQ EMASRGLWDS FRQSERGEET 200
IEERFEITGT MRKLADQSLP PNFSSLENFR AYVDGFEPNG YIEGKLSQMS 250
KEVNARIEPF LKSTPRPLRL PDGPPCSQRS KFLLMDALKL SIEDPSHEGE 300
GIPLYDAIKC MRTFFGWKEP NVVKPHEKGI NPNYLLSWKQ VLAELQDIEN 350
EEKIPRTKNM KKTSQLKWAL GENMAPEKVD FDDCKDVGDL KQYDSDEPEL 400
RSLASWIQNE FNKACELTDS SWIELDEIGE DAAPIEHIAS MRRNYFTAEV 450
SHCRATEYIM KGVYINTALL NASCAAMDDF QLIPMISKCR TKEGRRKTNL 500
YGFIIKGRSH LRNDTDVVNF VSMEFSLTDP RLEPHKWEKY CVLEVGDMLL 550
RSAIGHVSRP MFLYVRTNGT SKIKMKWGME MRRCLLQSLQ QIESMIEAES 600
SVKEKDMTKE FFENKSETWP VGESPKGVEE GSIGKVCRTL LAKSVFNSLY 650
ASPQLEGFSA ESRKLLLIVQ ALRDNLEPGT FDLGGLYEAI EECLINDPWV 700
LLNASWFNSF LTHALR 716
Length:716
Mass (Da):82,531
Last modified:April 1, 1990 - v1
Checksum:i2854E89F8949AE5E
GO
Isoform PA-X (identifier: P0DJW8-1) [UniParc]FASTAAdd to Basket

The sequence of this isoform can be found in the external entry P0DJW8.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:252
Mass (Da):29,391
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X17336 Genomic RNA. Translation: CAA35212.1.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X17336 Genomic RNA. Translation: CAA35212.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4IUJ X-ray 1.90 A 254-716 [» ]
ProteinModelPortali P15659.
SMRi P15659. Positions 257-716.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P15659. 2 interactions.
MINTi MINT-8081657.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR001009. RNA-dir_pol_influenzavirus.
[Graphical view ]
Pfami PF00603. Flu_PA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of the PA gene of influenza A/WSN/33(H1N1)."
    Odagiri T., Tobita K.
    Nucleic Acids Res. 18:654-654(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Involvement of influenza virus PA subunit in assembly of functional RNA polymerase complexes."
    Kawaguchi A., Naito T., Nagata K.
    J. Virol. 79:732-744(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF LEU-226.

Entry informationi

Entry nameiPA_I33A0
AccessioniPrimary (citable) accession number: P15659
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: July 9, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi