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P15659

- PA_I33A0

UniProt

P15659 - PA_I33A0

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Protein

Polymerase acidic protein

Gene

PA

Organism
Influenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1))
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Plays an essential role in viral RNA transcription and replication by forming the heterotrimeric polymerase complex together with PB1 and PB2 subunits. The complex transcribes viral mRNAs by using a unique mechanism called cap-snatching. It consists in the hijacking and cleavage of host capped pre-mRNAs. These short capped RNAs are then used as primers for viral mRNAs. The PB2 subunit is responsible for the binding of the 5' cap of cellular pre-mRNAs which are subsequently cleaved after 10-13 nucleotides by the PA subunit that carries the endonuclease activity. In addition of its function in viral transcription, PA also plays an essential role in viral RNA synthesis and promotes the formation of the trimeric polymerase complex.By similarity

GO - Molecular functioni

  1. RNA binding Source: InterPro
  2. RNA-directed RNA polymerase activity Source: InterPro

GO - Biological processi

  1. suppression by virus of host RNA polymerase II activity Source: UniProtKB-KW
  2. transcription, DNA-templated Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Cap snatching, Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host RNA polymerase II by virus

Names & Taxonomyi

Protein namesi
Recommended name:
Polymerase acidic protein
Alternative name(s):
RNA-directed RNA polymerase subunit P2
Gene namesi
Name:PA
OrganismiInfluenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1))
Taxonomic identifieri381518 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000000834: Genome

Subcellular locationi

Host cytoplasm. Host nucleus
Note: PB1 and PA are transported in the host nucleus as a complex.By similarity

Keywords - Cellular componenti

Host cytoplasm, Host nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi226 – 2261L → P: Confers temperature sensitivity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 716716Polymerase acidic proteinPRO_0000078801Add
BLAST

Post-translational modificationi

Phosphorylated on serines and threonines by host kinases, including human casein kinase II.By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA. Interacts (via C-terminus) with PB1 (via N-terminus).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
USP11P517842EBI-8431752,EBI-306876From a different organism.

Protein-protein interaction databases

IntActiP15659. 2 interactions.
MINTiMINT-8081657.

Structurei

Secondary structure

1
716
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi273 – 2753Combined sources
Beta strandi290 – 2945Combined sources
Turni298 – 3014Combined sources
Helixi303 – 31210Combined sources
Beta strandi317 – 3248Combined sources
Helixi331 – 35020Combined sources
Beta strandi351 – 3533Combined sources
Beta strandi356 – 3605Combined sources
Helixi364 – 3696Combined sources
Helixi406 – 41510Combined sources
Beta strandi419 – 4213Combined sources
Helixi437 – 45014Combined sources
Helixi454 – 47522Combined sources
Turni476 – 4783Combined sources
Beta strandi479 – 49012Combined sources
Beta strandi496 – 50611Combined sources
Beta strandi517 – 52610Combined sources
Helixi534 – 5374Combined sources
Beta strandi541 – 55010Combined sources
Beta strandi555 – 57117Combined sources
Helixi572 – 5798Combined sources
Helixi580 – 5823Combined sources
Helixi583 – 60321Combined sources
Helixi608 – 6125Combined sources
Helixi633 – 64917Combined sources
Helixi653 – 67523Combined sources
Helixi686 – 6916Combined sources
Helixi698 – 71215Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4IUJX-ray1.90A254-716[»]
ProteinModelPortaliP15659.
SMRiP15659. Positions 257-716.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni124 – 13916Nuclear localization signal 1 (NLS1)By similarityAdd
BLAST
Regioni184 – 24764Nuclear localization signal 2 (NLS2)By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the influenza viruses PA family.Curated

Family and domain databases

InterProiIPR001009. RNA-dir_pol_influenzavirus.
[Graphical view]
PfamiPF00603. Flu_PA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by ribosomal frameshifting. Align

Isoform PA (identifier: P15659-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEDFVRQCFN PMIVELAEKA MKEYGEDLKI ETNKFAAICT HLEVCFMYSD
60 70 80 90 100
FHFIDEQGES IVVELGDPNA LLKHRFEIIE GRDRTIAWTV INSICNTTGA
110 120 130 140 150
EKPKFLPDLY DYKKNRFIEI GVTRREVHIY YLEKANKIKS EKTHIHIFSF
160 170 180 190 200
TGEEMATKAD YTLDEESRAR IKTRLFTIRQ EMASRGLWDS FRQSERGEET
210 220 230 240 250
IEERFEITGT MRKLADQSLP PNFSSLENFR AYVDGFEPNG YIEGKLSQMS
260 270 280 290 300
KEVNARIEPF LKSTPRPLRL PDGPPCSQRS KFLLMDALKL SIEDPSHEGE
310 320 330 340 350
GIPLYDAIKC MRTFFGWKEP NVVKPHEKGI NPNYLLSWKQ VLAELQDIEN
360 370 380 390 400
EEKIPRTKNM KKTSQLKWAL GENMAPEKVD FDDCKDVGDL KQYDSDEPEL
410 420 430 440 450
RSLASWIQNE FNKACELTDS SWIELDEIGE DAAPIEHIAS MRRNYFTAEV
460 470 480 490 500
SHCRATEYIM KGVYINTALL NASCAAMDDF QLIPMISKCR TKEGRRKTNL
510 520 530 540 550
YGFIIKGRSH LRNDTDVVNF VSMEFSLTDP RLEPHKWEKY CVLEVGDMLL
560 570 580 590 600
RSAIGHVSRP MFLYVRTNGT SKIKMKWGME MRRCLLQSLQ QIESMIEAES
610 620 630 640 650
SVKEKDMTKE FFENKSETWP VGESPKGVEE GSIGKVCRTL LAKSVFNSLY
660 670 680 690 700
ASPQLEGFSA ESRKLLLIVQ ALRDNLEPGT FDLGGLYEAI EECLINDPWV
710
LLNASWFNSF LTHALR
Length:716
Mass (Da):82,531
Last modified:April 1, 1990 - v1
Checksum:i2854E89F8949AE5E
GO
Isoform PA-X (identifier: P0DJW8-1) [UniParc]FASTAAdd to Basket

The sequence of this isoform can be found in the external entry P0DJW8.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:252
Mass (Da):29,391
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17336 Genomic RNA. Translation: CAA35212.1.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17336 Genomic RNA. Translation: CAA35212.1 .

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4IUJ X-ray 1.90 A 254-716 [» ]
ProteinModelPortali P15659.
SMRi P15659. Positions 257-716.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P15659. 2 interactions.
MINTi MINT-8081657.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR001009. RNA-dir_pol_influenzavirus.
[Graphical view ]
Pfami PF00603. Flu_PA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of the PA gene of influenza A/WSN/33(H1N1)."
    Odagiri T., Tobita K.
    Nucleic Acids Res. 18:654-654(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Involvement of influenza virus PA subunit in assembly of functional RNA polymerase complexes."
    Kawaguchi A., Naito T., Nagata K.
    J. Virol. 79:732-744(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF LEU-226.

Entry informationi

Entry nameiPA_I33A0
AccessioniPrimary (citable) accession number: P15659
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: November 26, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3