Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P15659 (PA_I33A0) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Polymerase acidic protein
Alternative name(s):
RNA-directed RNA polymerase subunit P2
Gene names
Name:PA
OrganismInfluenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1)) [Complete proteome]
Taxonomic identifier381518 [NCBI]
Taxonomic lineageVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length716 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Implicated in endonuclease cleavage of capped RNA primers. Displays an elongation factor activity in viral RNA synthesis. Dispensable for viral transcription, but not replication.

Subunit structure

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA.

Post-translational modification

Phosphorylated on serines and threonines by host kinases, including human casein kinase II.

Sequence similarities

Belongs to the influenza viruses PA family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

USP11P517842EBI-8431752,EBI-306876From a different organism.

Alternative products

This entry describes 2 isoforms produced by ribosomal frameshifting. [Align] [Select]
Isoform PA (identifier: P15659-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform PA-X (identifier: P0DJW8-1)

The sequence of this isoform can be found in the external entry P0DJW8.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 716716Polymerase acidic protein
PRO_0000078801

Experimental info

Mutagenesis2261L → P: Confers temperature sensitivity. Ref.2

Secondary structure

................................................... 716
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform PA [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: 2854E89F8949AE5E

FASTA71682,531
        10         20         30         40         50         60 
MEDFVRQCFN PMIVELAEKA MKEYGEDLKI ETNKFAAICT HLEVCFMYSD FHFIDEQGES 

        70         80         90        100        110        120 
IVVELGDPNA LLKHRFEIIE GRDRTIAWTV INSICNTTGA EKPKFLPDLY DYKKNRFIEI 

       130        140        150        160        170        180 
GVTRREVHIY YLEKANKIKS EKTHIHIFSF TGEEMATKAD YTLDEESRAR IKTRLFTIRQ 

       190        200        210        220        230        240 
EMASRGLWDS FRQSERGEET IEERFEITGT MRKLADQSLP PNFSSLENFR AYVDGFEPNG 

       250        260        270        280        290        300 
YIEGKLSQMS KEVNARIEPF LKSTPRPLRL PDGPPCSQRS KFLLMDALKL SIEDPSHEGE 

       310        320        330        340        350        360 
GIPLYDAIKC MRTFFGWKEP NVVKPHEKGI NPNYLLSWKQ VLAELQDIEN EEKIPRTKNM 

       370        380        390        400        410        420 
KKTSQLKWAL GENMAPEKVD FDDCKDVGDL KQYDSDEPEL RSLASWIQNE FNKACELTDS 

       430        440        450        460        470        480 
SWIELDEIGE DAAPIEHIAS MRRNYFTAEV SHCRATEYIM KGVYINTALL NASCAAMDDF 

       490        500        510        520        530        540 
QLIPMISKCR TKEGRRKTNL YGFIIKGRSH LRNDTDVVNF VSMEFSLTDP RLEPHKWEKY 

       550        560        570        580        590        600 
CVLEVGDMLL RSAIGHVSRP MFLYVRTNGT SKIKMKWGME MRRCLLQSLQ QIESMIEAES 

       610        620        630        640        650        660 
SVKEKDMTKE FFENKSETWP VGESPKGVEE GSIGKVCRTL LAKSVFNSLY ASPQLEGFSA 

       670        680        690        700        710 
ESRKLLLIVQ ALRDNLEPGT FDLGGLYEAI EECLINDPWV LLNASWFNSF LTHALR 

« Hide

Isoform PA-X [UniParc].

See P0DJW8.

References

[1]"Nucleotide sequence of the PA gene of influenza A/WSN/33(H1N1)."
Odagiri T., Tobita K.
Nucleic Acids Res. 18:654-654(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Involvement of influenza virus PA subunit in assembly of functional RNA polymerase complexes."
Kawaguchi A., Naito T., Nagata K.
J. Virol. 79:732-744(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF LEU-226.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X17336 Genomic RNA. Translation: CAA35212.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4IUJX-ray1.90A254-716[»]
ProteinModelPortalP15659.
SMRP15659. Positions 257-716.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP15659. 2 interactions.
MINTMINT-8081657.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR001009. RNA-dir_pol_influenzavirus.
[Graphical view]
PfamPF00603. Flu_PA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePA_I33A0
AccessionPrimary (citable) accession number: P15659
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: April 16, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references