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P15659

- PA_I33A0

UniProt

P15659 - PA_I33A0

Protein

Polymerase acidic protein

Gene

PA

Organism
Influenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1))
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (01 Apr 1990)
      Previous versions | rss
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    Functioni

    Implicated in endonuclease cleavage of capped RNA primers. Displays an elongation factor activity in viral RNA synthesis. Dispensable for viral transcription, but not replication.

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. RNA binding Source: InterPro
    3. RNA-directed RNA polymerase activity Source: InterPro

    GO - Biological processi

    1. suppression by virus of host RNA polymerase II activity Source: UniProtKB-KW
    2. transcription, DNA-templated Source: InterPro

    Keywords - Biological processi

    Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host RNA polymerase II by virus

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polymerase acidic protein
    Alternative name(s):
    RNA-directed RNA polymerase subunit P2
    Gene namesi
    Name:PA
    OrganismiInfluenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1))
    Taxonomic identifieri381518 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
    Virus hostiAves [TaxID: 8782]
    Homo sapiens (Human) [TaxID: 9606]
    Sus scrofa (Pig) [TaxID: 9823]
    ProteomesiUP000000834: Genome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi226 – 2261L → P: Confers temperature sensitivity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 716716Polymerase acidic proteinPRO_0000078801Add
    BLAST

    Post-translational modificationi

    Phosphorylated on serines and threonines by host kinases, including human casein kinase II.

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Subunit structurei

    Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    USP11P517842EBI-8431752,EBI-306876From a different organism.

    Protein-protein interaction databases

    IntActiP15659. 2 interactions.
    MINTiMINT-8081657.

    Structurei

    Secondary structure

    1
    716
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi273 – 2753
    Beta strandi290 – 2945
    Turni298 – 3014
    Helixi303 – 31210
    Beta strandi317 – 3248
    Helixi331 – 35020
    Beta strandi351 – 3533
    Beta strandi356 – 3605
    Helixi364 – 3696
    Helixi406 – 41510
    Beta strandi419 – 4213
    Helixi437 – 45014
    Helixi454 – 47522
    Turni476 – 4783
    Beta strandi479 – 49012
    Beta strandi496 – 50611
    Beta strandi517 – 52610
    Helixi534 – 5374
    Beta strandi541 – 55010
    Beta strandi555 – 57117
    Helixi572 – 5798
    Helixi580 – 5823
    Helixi583 – 60321
    Helixi608 – 6125
    Helixi633 – 64917
    Helixi653 – 67523
    Helixi686 – 6916
    Helixi698 – 71215

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4IUJX-ray1.90A254-716[»]
    ProteinModelPortaliP15659.
    SMRiP15659. Positions 257-716.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the influenza viruses PA family.Curated

    Family and domain databases

    InterProiIPR001009. RNA-dir_pol_influenzavirus.
    [Graphical view]
    PfamiPF00603. Flu_PA. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by ribosomal frameshifting. Align

    Isoform PA (identifier: P15659-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEDFVRQCFN PMIVELAEKA MKEYGEDLKI ETNKFAAICT HLEVCFMYSD    50
    FHFIDEQGES IVVELGDPNA LLKHRFEIIE GRDRTIAWTV INSICNTTGA 100
    EKPKFLPDLY DYKKNRFIEI GVTRREVHIY YLEKANKIKS EKTHIHIFSF 150
    TGEEMATKAD YTLDEESRAR IKTRLFTIRQ EMASRGLWDS FRQSERGEET 200
    IEERFEITGT MRKLADQSLP PNFSSLENFR AYVDGFEPNG YIEGKLSQMS 250
    KEVNARIEPF LKSTPRPLRL PDGPPCSQRS KFLLMDALKL SIEDPSHEGE 300
    GIPLYDAIKC MRTFFGWKEP NVVKPHEKGI NPNYLLSWKQ VLAELQDIEN 350
    EEKIPRTKNM KKTSQLKWAL GENMAPEKVD FDDCKDVGDL KQYDSDEPEL 400
    RSLASWIQNE FNKACELTDS SWIELDEIGE DAAPIEHIAS MRRNYFTAEV 450
    SHCRATEYIM KGVYINTALL NASCAAMDDF QLIPMISKCR TKEGRRKTNL 500
    YGFIIKGRSH LRNDTDVVNF VSMEFSLTDP RLEPHKWEKY CVLEVGDMLL 550
    RSAIGHVSRP MFLYVRTNGT SKIKMKWGME MRRCLLQSLQ QIESMIEAES 600
    SVKEKDMTKE FFENKSETWP VGESPKGVEE GSIGKVCRTL LAKSVFNSLY 650
    ASPQLEGFSA ESRKLLLIVQ ALRDNLEPGT FDLGGLYEAI EECLINDPWV 700
    LLNASWFNSF LTHALR 716
    Length:716
    Mass (Da):82,531
    Last modified:April 1, 1990 - v1
    Checksum:i2854E89F8949AE5E
    GO
    Isoform PA-X (identifier: P0DJW8-1) [UniParc]FASTAAdd to Basket

    The sequence of this isoform can be found in the external entry P0DJW8.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
    Length:252
    Mass (Da):29,391
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17336 Genomic RNA. Translation: CAA35212.1.

    Keywords - Coding sequence diversityi

    Ribosomal frameshifting

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17336 Genomic RNA. Translation: CAA35212.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4IUJ X-ray 1.90 A 254-716 [» ]
    ProteinModelPortali P15659.
    SMRi P15659. Positions 257-716.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P15659. 2 interactions.
    MINTi MINT-8081657.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR001009. RNA-dir_pol_influenzavirus.
    [Graphical view ]
    Pfami PF00603. Flu_PA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the PA gene of influenza A/WSN/33(H1N1)."
      Odagiri T., Tobita K.
      Nucleic Acids Res. 18:654-654(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Involvement of influenza virus PA subunit in assembly of functional RNA polymerase complexes."
      Kawaguchi A., Naito T., Nagata K.
      J. Virol. 79:732-744(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF LEU-226.

    Entry informationi

    Entry nameiPA_I33A0
    AccessioniPrimary (citable) accession number: P15659
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: April 1, 1990
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3