P15655 (FGF2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fibroblast growth factor 2
Short name=FGF-2

Alternative name(s):
Basic fibroblast growth factor
Short name=bFGF
Heparin-binding growth factor 2
Short name=HBGF-2

Gene names

Name:	Fgf2
Synonyms:	Fgf-2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	154 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro By similarity.
Subunit structure	Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2 By similarity. Ref.3
Subcellular location	Secreted By similarity. Nucleus By similarity. Note: Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane By similarity. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57 By similarity.
Post-translational modification	Phosphorylation at Tyr-81 regulates FGF2 unconventional secretion By similarity.
Sequence similarities	Belongs to the heparin-binding growth factors family.

Ontologies

Keywords
Biological process	Angiogenesis Differentiation
Cellular component	Nucleus Secreted
Ligand	Heparin-binding
Molecular function	Developmental protein Growth factor Mitogen
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	C21-steroid hormone biosynthetic process Inferred from electronic annotation. Source: Compara MAPK import into nucleus Inferred from electronic annotation. Source: Compara activation of MAPKK activity Inferred from direct assay PubMed 16339969. Source: MGI angiogenesis Inferred from direct assay PubMed 14702107PubMed 14998491. Source: MGI apoptotic process Inferred from direct assay PubMed 8050499. Source: MGI branching involved in ureteric bud morphogenesis Inferred from sequence or structural similarity. Source: UniProtKB cardiac muscle cell proliferation Traceable author statement PubMed 20299672. Source: DFLAT cell migration involved in sprouting angiogenesis Inferred from electronic annotation. Source: Compara chondroblast differentiation Inferred from electronic annotation. Source: Compara corticotropin hormone secreting cell differentiation Inferred from direct assay PubMed 9463347. Source: MGI embryo development Inferred from electronic annotation. Source: Compara fibroblast growth factor receptor signaling pathway Inferred from genetic interaction PubMed 7494107. Source: MGI glial cell differentiation Inferred from mutant phenotype PubMed 12878680. Source: MGI hyaluronan catabolic process Inferred from electronic annotation. Source: Compara inositol phosphate biosynthetic process Inferred from electronic annotation. Source: Compara lung development Inferred from direct assay PubMed 15576401. Source: MGI mammary gland epithelial cell differentiation Inferred from direct assay PubMed 1376141. Source: MGI negative regulation of blood vessel endothelial cell migration Inferred from electronic annotation. Source: Compara negative regulation of cell death Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of cell growth Inferred from electronic annotation. Source: Compara negative regulation of cell proliferation Inferred from genetic interaction PubMed 10027293PubMed 20570969. Source: MGI negative regulation of fibroblast migration Inferred from electronic annotation. Source: Compara negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: Compara negative regulation of wound healing Inferred from electronic annotation. Source: Compara organ induction Inferred from direct assay PubMed 15576401. Source: MGI phosphatidylinositol biosynthetic process Inferred from electronic annotation. Source: Compara positive regulation of ERK1 and ERK2 cascade Inferred from electronic annotation. Source: Compara positive regulation of angiogenesis Inferred from direct assay PubMed 16648142. Source: MGI positive regulation of blood vessel endothelial cell migration Inferred from electronic annotation. Source: Compara positive regulation of cardiac muscle cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of cell division Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of cell fate specification Inferred from sequence orthology PubMed 18635606. Source: MGI positive regulation of cerebellar granule cell precursor proliferation Inferred from direct assay PubMed 10027293. Source: MGI positive regulation of endothelial cell migration Inferred from direct assay PubMed 16291864. Source: MGI positive regulation of endothelial cell proliferation Inferred from direct assay PubMed 16291864. Source: MGI positive regulation of osteoblast differentiation Inferred from genetic interaction PubMed 20570969. Source: MGI positive regulation of phosphatidylinositol 3-kinase activity Inferred from electronic annotation. Source: Compara positive regulation of phospholipase C activity Inferred from electronic annotation. Source: Compara positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 10391928PubMed 9102311. Source: MGI regulation of cell cycle Inferred from direct assay PubMed 9463347. Source: MGI regulation of retinal cell programmed cell death Inferred from direct assay PubMed 11747369. Source: MGI release of sequestered calcium ion into cytosol Inferred from electronic annotation. Source: Compara response to axon injury Inferred from mutant phenotype PubMed 16009496. Source: MGI stem cell development Inferred from mutant phenotype PubMed 12843296. Source: BHF-UCL substantia nigra development Inferred from mutant phenotype PubMed 17234579. Source: MGI thyroid-stimulating hormone-secreting cell differentiation Inferred from direct assay PubMed 9463347. Source: MGI wound healing Inferred from electronic annotation. Source: Compara
Cellular_component	cytosol Inferred from direct assay PubMed 10644718. Source: MGI extracellular space Inferred from direct assay PubMed 12417662PubMed 15576401. Source: MGI nucleus Inferred from direct assay PubMed 10644718. Source: MGI
Molecular_function	chemoattractant activity Inferred from electronic annotation. Source: Compara growth factor activity Inferred from direct assay PubMed 15608665. Source: MGI heparin binding Inferred from electronic annotation. Source: UniProtKB-KW ligand-dependent nuclear receptor transcription coactivator activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 9	9			PRO_0000008936
	Chain	10 – 154	145	Fibroblast growth factor 2		PRO_0000008937
Regions
	Region	127 – 143	17	Heparin-binding By similarity
Sites
	Binding site	35	1	Heparin By similarity
Amino acid modifications
	Modified residue	81	1	Phosphotyrosine; by TEC By similarity

Sequences

	Sequence		Length	Mass (Da)
	P15655 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: 689F677416274388	FASTA	154	17,153
	10 20 30 40 50 60 MAASGITSLP ALPEDGGAAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHVK 70 80 90 100 110 120 LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTEEC FFFERLESNN YNTYRSRKYS 130 140 150 SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS

References

[1]	"Isolation of cDNAs encoding four mouse FGF family members and characterization of their expression patterns during embryogenesis." Hebert J.M., Basilico C., Goldfarb M., Haub O., Martin G.R. Dev. Biol. 138:454-463(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Ma R.Z., Teuscher C. Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: A/J, C57BL/6J and NOD/LtJ. Tissue: Spleen.
[3]	"Expression of a binding protein for FGF is associated with epithelial development and skin carcinogenesis." Kurtz A., Wang H.-L., Darwiche N., Harris V., Wellstein A. Oncogene 14:2671-2681(1997) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH FGF2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M30644 mRNA. Translation: AAA37621.1. AF065903 mRNA. Translation: AAC17503.1. AF065904 mRNA. Translation: AAC17504.1. AF065905 mRNA. Translation: AAC17505.1.
IPI	IPI00132609.
PIR	C37360.
RefSeq	NP_032032.1. NM_008006.2.
UniGene	Mm.473689.
3D structure databases
ProteinModelPortal	P15655.
SMR	P15655. Positions 1-154.
ModBase	Search...
PTM databases
PhosphoSite	P15655.
Proteomic databases
PRIDE	P15655.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000038885; ENSMUSP00000037694; ENSMUSG00000037225. ENSMUST00000138563; ENSMUSP00000122227; ENSMUSG00000037225.
GeneID	14173.
KEGG	mmu:14173.
Organism-specific databases
CTD	2247.
MGI	MGI:95516. Fgf2.
Phylogenomic databases
eggNOG	NOG325757.
HOGENOM	HOG000236341.
HOVERGEN	HBG007580.
InParanoid	P15655.
KO	K04358.
OMA	LPMSAKC.
Gene expression databases
ArrayExpress	P15655.
Bgee	P15655.
CleanEx	MM_FGF2.
Genevestigator	P15655.
GermOnline	ENSMUSG00000037225. Mus musculus.
Family and domain databases
InterPro	IPR008996. Cytokine_IL1-like. IPR002209. GF_heparin-bd. IPR002348. IL1_HBGF. [Graphical view]
PANTHER	PTHR11486. PTHR11486. 1 hit.
Pfam	PF00167. FGF. 1 hit. [Graphical view]
PRINTS	PR00263. HBGFFGF. PR00262. IL1HBGF.
SMART	SM00442. FGF. 1 hit. [Graphical view]
SUPFAM	SSF50353. Cytok_IL1_like. 1 hit.
PROSITE	PS00247. HBGF_FGF. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	285342.
SOURCE	Search...

Entry information

Entry name

FGF2_MOUSE

Accession

Primary (citable) accession number: P15655

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	April 3, 2013
This is version 127 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program