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P15640 (PUR2_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:b4005, JW3969
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length429 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Subunit structure

Monomer.

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 429429Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_0000151448

Regions

Domain109 – 316208ATP-grasp
Nucleotide binding135 – 19662ATP By similarity

Sites

Metal binding2861Magnesium or manganese By similarity
Metal binding2881Magnesium or manganese By similarity

Experimental info

Sequence conflict15 – 162LA → WR in AAA24455. Ref.1
Sequence conflict971G → V in AAA24455. Ref.1

Secondary structure

...................................................................... 429
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P15640 [UniParc].

Last modified October 1, 1993. Version 2.
Checksum: 5E9EFAD478BC1FF4

FASTA42945,940
        10         20         30         40         50         60 
MKVLVIGNGG REHALAWKAA QSPLVETVFV APGNAGTALE PALQNVAIGV TDIPALLDFA 

        70         80         90        100        110        120 
QNEKIDLTIV GPEAPLVKGV VDTFRAAGLK IFGPTAGAAQ LEGSKAFTKD FLARHKIPTA 

       130        140        150        160        170        180 
EYQNFTEVEP ALAYLREKGA PIVIKADGLA AGKGVIVAMT LEEAEAAVHD MLAGNAFGDA 

       190        200        210        220        230        240 
GHRIVIEEFL DGEEASFIVM VDGEHVLPMA TSQDHKRVGD KDTGPNTGGM GAYSPAPVVT 

       250        260        270        280        290        300 
DDVHQRTMER IIWPTVKGMA AEGNTYTGFL YAGLMIDKQG NPKVIEFNCR FGDPETQPIM 

       310        320        330        340        350        360 
LRMKSDLVEL CLAACESKLD EKTSEWDERA SLGVVMAAGG YPGDYRTGDV IHGLPLEEVA 

       370        380        390        400        410        420 
GGKVFHAGTK LADDEQVVTN GGRVLCVTAL GHTVAEAQKR AYALMTDIHW DDCFCRKDIG 


WRAIEREQN 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence analysis of genes purH and purD involved in the de novo purine nucleotide biosynthesis of Escherichia coli."
Aiba A., Mizobuchi K.
J. Biol. Chem. 264:21239-21246(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Glycinamide ribonucleotide synthetase from Escherichia coli: cloning, overproduction, sequencing, isolation, and characterization."
Shen Y., Rudolph J., Stern M., Flannigan K.A., Smith J.M.
Biochemistry 29:218-227(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[3]"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes."
Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.
Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"X-ray crystal structure of glycinamide ribonucleotide synthetase from Escherichia coli."
Wang W., Kappock T.J., Stubbe J., Ealick S.E.
Biochemistry 37:15647-15662(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J05126 Genomic DNA. Translation: AAA24455.1.
X51950 Genomic DNA. Translation: CAA36213.1.
U00006 Genomic DNA. Translation: AAC43103.1.
U00096 Genomic DNA. Translation: AAC76979.1.
AP009048 Genomic DNA. Translation: BAE77314.1.
PIRAJECQG. A33771.
RefSeqNP_418433.1. NC_000913.3.
YP_491455.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1GSOX-ray1.60A1-429[»]
ProteinModelPortalP15640.
SMRP15640. Positions 1-426.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP15640. 4 interactions.
STRING511145.b4005.

2D gel databases

SWISS-2DPAGEP15640.

Proteomic databases

PaxDbP15640.
PRIDEP15640.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76979; AAC76979; b4005.
BAE77314; BAE77314; BAE77314.
GeneID12933468.
948504.
KEGGecj:Y75_p3191.
eco:b4005.
PATRIC32123535. VBIEscCol129921_4120.

Organism-specific databases

EchoBASEEB0785.
EcoGeneEG10792. purD.

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033463.
KOK01945.
OMADPEAMNV.
OrthoDBEOG69SKD1.
PhylomeDBP15640.
ProtClustDBPRK00885.

Enzyme and pathway databases

BioCycEcoCyc:GLYCRIBONUCSYN-MONOMER.
ECOL316407:JW3969-MONOMER.
MetaCyc:GLYCRIBONUCSYN-MONOMER.
UniPathwayUPA00074; UER00125.

Gene expression databases

GenevestigatorP15640.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP15640.
PROP15640.

Entry information

Entry namePUR2_ECOLI
AccessionPrimary (citable) accession number: P15640
Secondary accession number(s): Q2M8U2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: October 1, 1993
Last modified: April 16, 2014
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene