Reviewed, UniProtKB/Swiss-Prot P15638 (URT2_DESRO)

Names and origin

Protein names	Recommended name:     Salivary plasminogen activator alpha 2     EC=3.4.21.68 Alternative name(s):     DSPA alpha-2     BAT-PA     T-plasminogen activator
Organism	Desmodus rotundus (Vampire bat)
Taxonomic identifier	9430 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Chiroptera › Microchiroptera › Phyllostomidae › Desmodontinae › Desmodus

Protein attributes

Sequence length	477 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probably essential to support the feeding habits of this exclusively haematophagous animal. Probable potent thrombolytic agent.
Catalytic activity	Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.
Enzyme regulation	Activity toward plasminogen is stimulated in the presence of fibrin I.
Subunit structure	Monomer.
Subcellular location	Secreted.
Domain	The fibronectin type-I domain mediates binding to fibrin, and the kringle domain apparently mediates fibrin-induced stimulation of activity.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 EGF-like domain. Contains 1 fibronectin type-I domain. Contains 1 kringle domain. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Plasminogen activation
Cellular component	Secreted
Domain	EGF-like domain Kringle Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 36	36	Potential
	Chain	37 – 477	441	Salivary plasminogen activator alpha 2		PRO_0000028341
Regions
	Domain	40 – 82	43	Fibronectin type-I
	Domain	83 – 121	39	EGF-like
	Domain	128 – 209	82	Kringle
	Domain	226 – 476	251	Peptidase S1
Sites
	Active site	272	1	Charge relay system By similarity
	Active site	321	1	Charge relay system By similarity
	Active site	428	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	185	1	N-linked (GlcNAc...) Potential
	Glycosylation	398	1	N-linked (GlcNAc...) Potential
	Disulfide bond	42 ↔ 72		By similarity
	Disulfide bond	70 ↔ 79		By similarity
	Disulfide bond	87 ↔ 98		By similarity
	Disulfide bond	92 ↔ 109		By similarity
	Disulfide bond	111 ↔ 120		By similarity
	Disulfide bond	128 ↔ 209		By similarity
	Disulfide bond	149 ↔ 191		By similarity
	Disulfide bond	180 ↔ 204		By similarity
	Disulfide bond	214 ↔ 345		By similarity
	Disulfide bond	257 ↔ 273		By similarity
	Disulfide bond	265 ↔ 334		By similarity
	Disulfide bond	359 ↔ 434		By similarity
	Disulfide bond	391 ↔ 407		By similarity
	Disulfide bond	424 ↔ 452		By similarity
Experimental info
	Sequence conflict	403	1	N → K in AAA31596. Ref.2
	Sequence conflict	417	1	Y → H in AAA31596. Ref.2
	Sequence conflict	435	1	M → R in AAA31596. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P15638-1 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 17486555C0E5077C	FASTA	477	53,719
	10 20 30 40 50 60 MVNTMKTKLL CVLLLCGAVF SLPRQETYRQ LARGSRAYGV ACRDEKTQMI YQQQESWLRP 70 80 90 100 110 120 EVRSKRVEHC RCDRGLAQCH TVPVKSCSEL RCFNGGTCWQ AASFSDFVCQ CPKGYTGKQC 130 140 150 160 170 180 EVDTHATCYK DQGVTYRGTW STSESGAQCI NWNSNLLTRR TYNGRRSDAI TLGLGNHNYC 190 200 210 220 230 240 RNPDNNSKPW CYVIKASKFI LEFCSVPVCS KATCGLRKYK EPQLHSTGGL FTDITSHPWQ 250 260 270 280 290 300 AAIFAQNRRS SGERFLCGGI LISSCWVLTA AHCFQERYPP QHLRVVLGRT YRVKPGKEEQ 310 320 330 340 350 360 TFEVEKCIVH EEFDDDTYNN DIALLQLKSG SPQCAQESDS VRAICLPEAN LQLPDWTECE 370 380 390 400 410 420 LSGYGKHKSS SPFYSEQLKE GHVRLYPSSR CTSKFLFNKT VTNNMLCAGD TRSGEIYPNV 430 440 450 460 470 HDACQGDSGG PLVCMNDNHM TLLGIISWGV GCGEKDIPGV YTKVTNYLGW IRDNMRP

References

[1]	"The plasminogen activator family from the salivary gland of the vampire bat Desmodus rotundus: cloning and expression." Kraetzschmar J., Haendler B., Langer G., Boidol W., Bringmann P., Alagon A., Donner P., Schleuning W.-D. Gene 105:229-237(1991) [PubMed: 1937019] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Salivary gland.
[2]	"Isolation, characterization, and cDNA cloning of a vampire bat salivary plasminogen activator." Gardell S.J., Duong L.T., Diehl R.E., York J.D., Hare T.R., Register R.B., Jacobs J.W., Dixon R.A.F., Friedman P.A. J. Biol. Chem. 264:17947-17952(1989) [PubMed: 2509450] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Salivary gland.
[3]	"Plasminogen activators from the saliva of Desmodus rotundus (common vampire bat): unique fibrin specificity." Schleuning W.-D., Alagon A., Boidol W., Bringmann P., Petri T., Kraetzschmar J., Haendler B., Langer G., Baldus B., Witt W., Donner P. Ann. N. Y. Acad. Sci. 667:395-403(1992) [PubMed: 1309059] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M63988 mRNA. Translation: AAA31593.1. J05082 mRNA. Translation: AAA31596.1.
PIR	A34369. JS0598.
3D structure databases
HSSP	HSSP built from PDB template 1A5I based on UniProtKB P98119.
SMR	P15638. Positions 37-127, 213-475.
ModBase	Search...
Protein family/group databases
MEROPS	S01.239.
Phylogenomic databases
HOVERGEN	P15638.
Enzyme and pathway databases
BRENDA	3.4.21.68. 291428.
Family and domain databases
InterPro	IPR016060. Complement_control_module. IPR006209. EGF. IPR006210. EGF-like. IPR013032. EGF-like_reg_CS. IPR000742. EGF_3. IPR000083. Fibrnctn1. IPR000001. Kringle. IPR018056. Kringle_CS. IPR018059. Kringle_sub. IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Gene3D	G3DSA:2.10.70.10. Complement_control_module. 1 hit. G3DSA:2.40.20.10. Kringle. 1 hit.
Pfam	PF00008. EGF. 1 hit. PF00039. fn1. 1 hit. PF00051. Kringle. 1 hit. PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN. PR00018. KRINGLE.
ProDom	PD000395. Kringle. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00181. EGF. 1 hit. SM00058. FN1. 1 hit. SM00130. KR. 1 hit. SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS00022. EGF_1. 1 hit. PS01186. EGF_2. 1 hit. PS50026. EGF_3. 1 hit. PS01253. FN1_1. 1 hit. PS51091. FN1_2. 1 hit. PS00021. KRINGLE_1. 1 hit. PS50070. KRINGLE_2. 1 hit. PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

URT2_DESRO

Accession

Primary (citable) accession number: P15638

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)