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Protein

Protease 1

Gene
N/A
Organism
Achromobacter lyticus
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Preferential cleavage: Lys-|-Xaa, including Lys-|-Pro.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei262Charge relay systemBy similarity1
Active sitei318Charge relay systemBy similarity1
Active sitei399Charge relay systemBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BRENDAi3.4.21.50. 74.

Protein family/group databases

MEROPSiS01.280.

Names & Taxonomyi

Protein namesi
Recommended name:
Protease 1 (EC:3.4.21.50)
Alternative name(s):
API
Lysyl endopeptidase
Protease I
OrganismiAchromobacter lyticus
Taxonomic identifieri224 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeAchromobacter

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Or 27Sequence analysisAdd BLAST20
PropeptideiPRO_000002851521 – 2051 PublicationAdd BLAST185
ChainiPRO_0000028516206 – 473Protease 1Add BLAST268
PropeptideiPRO_0000028517474 – 653Thr/Ser-richAdd BLAST180

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi211 ↔ 4211 Publication
Disulfide bondi217 ↔ 2851 Publication
Disulfide bondi241 ↔ 2631 Publication

Post-translational modificationi

Three disulfide bonds are present.1 Publication

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PRIDEiP15636.

Structurei

Secondary structure

1653
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi218 – 220Combined sources3
Helixi226 – 229Combined sources4
Beta strandi230 – 235Combined sources6
Beta strandi238 – 246Combined sources9
Beta strandi256 – 260Combined sources5
Helixi261 – 263Combined sources3
Helixi268 – 272Combined sources5
Beta strandi275 – 277Combined sources3
Helixi291 – 294Combined sources4
Beta strandi304 – 306Combined sources3
Beta strandi308 – 314Combined sources7
Turni315 – 318Combined sources4
Beta strandi319 – 326Combined sources8
Helixi330 – 332Combined sources3
Beta strandi335 – 337Combined sources3
Beta strandi349 – 353Combined sources5
Helixi355 – 357Combined sources3
Beta strandi361 – 365Combined sources5
Beta strandi370 – 372Combined sources3
Beta strandi376 – 378Combined sources3
Beta strandi380 – 386Combined sources7
Beta strandi402 – 404Combined sources3
Beta strandi410 – 416Combined sources7
Helixi425 – 427Combined sources3
Beta strandi428 – 434Combined sources7
Helixi435 – 440Combined sources6
Helixi445 – 447Combined sources3
Helixi450 – 453Combined sources4
Beta strandi461 – 463Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ARBX-ray1.20A206-473[»]
1ARCX-ray2.00A206-473[»]
4GPGOther1.90A206-473[»]
ProteinModelPortaliP15636.
SMRiP15636.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15636.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini474 – 553PKDPROSITE-ProRule annotationAdd BLAST80

Domaini

The C-terminal extension has little effect on the function of API.

Sequence similaritiesi

Belongs to the peptidase S1 family.Curated
Contains 1 PKD domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

KOiK01337.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.670. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR017323. Lysyl_endo-pept.
IPR009003. Peptidase_S1_PA.
IPR022409. PKD/Chitinase_dom.
IPR000601. PKD_dom.
IPR002884. PrprotnconvertsP.
[Graphical view]
PfamiPF01483. P_proprotein. 1 hit.
PF00801. PKD. 1 hit.
[Graphical view]
PIRSFiPIRSF037930. Lysyl_endopeptidase. 1 hit.
SMARTiSM00089. PKD. 1 hit.
[Graphical view]
SUPFAMiSSF49299. SSF49299. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF50494. SSF50494. 1 hit.
PROSITEiPS50093. PKD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15636-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRICGSLLL LGLSISAALA APASRPAAFD YANLSSVDKV ALRTMPAVDV
60 70 80 90 100
AKAKAEDLQR DKRGDIPRFA LAIDVDMTPQ NSGAWEYTAD GQFAVWRQRV
110 120 130 140 150
RSEKALSLNF GFTDYYMPAG GRLLVYPATQ APAGDRGLIS QYDASNNNSA
160 170 180 190 200
RQLWTAVVPG AEAVIEAVIP RDKVGEFKLR LTKVNHDYVG FGPLARRLAA
210 220 230 240 250
ASGEKGVSGS CNIDVVCPEG DGRRDIIRAV GAYSKSGTLA CTGSLVNNTA
260 270 280 290 300
NDRKMYFLTA HHCGMGTAST AASIVVYWNY QNSTCRAPNT PASGANGDGS
310 320 330 340 350
MSQTQSGSTV KATYATSDFT LLELNNAANP AFNLFWAGWD RRDQNYPGAI
360 370 380 390 400
AIHHPNVAEK RISNSTSPTS FVAWGGGAGT THLNVQWQPS GGVTEPGSSG
410 420 430 440 450
SPIYSPEKRV LGQLHGGPSS CSATGTNRSD QYGRVFTSWT GGGAAASRLS
460 470 480 490 500
DWLDPASTGA QFIDGLDSGG GTPNTPPVAN FTSTTSGLTA TFTDSSTDSD
510 520 530 540 550
GSIASRSWNF GDGSTSTATN PSKTYAAAGT YTVTLTVTDN GGATNTKTGS
560 570 580 590 600
VTVSGGPGAQ TYTNDTDVAI PDNATVESPI TVSGRTGNGS ATTPIQVTIY
610 620 630 640 650
HTYKSDLKVD LVAPDGTVYN LHNRTGGSAH NIIQTFTKDL SSEAAQRAPG

SCG
Length:653
Mass (Da):68,125
Last modified:April 1, 1990 - v1
Checksum:i3A55159247EFFE7C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05128 Genomic DNA. Translation: AAA78946.1.
PIRiA32687.

Genome annotation databases

KEGGiag:AAA78946.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05128 Genomic DNA. Translation: AAA78946.1.
PIRiA32687.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ARBX-ray1.20A206-473[»]
1ARCX-ray2.00A206-473[»]
4GPGOther1.90A206-473[»]
ProteinModelPortaliP15636.
SMRiP15636.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS01.280.

Proteomic databases

PRIDEiP15636.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAA78946.

Phylogenomic databases

KOiK01337.

Enzyme and pathway databases

BRENDAi3.4.21.50. 74.

Miscellaneous databases

EvolutionaryTraceiP15636.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.670. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR017323. Lysyl_endo-pept.
IPR009003. Peptidase_S1_PA.
IPR022409. PKD/Chitinase_dom.
IPR000601. PKD_dom.
IPR002884. PrprotnconvertsP.
[Graphical view]
PfamiPF01483. P_proprotein. 1 hit.
PF00801. PKD. 1 hit.
[Graphical view]
PIRSFiPIRSF037930. Lysyl_endopeptidase. 1 hit.
SMARTiSM00089. PKD. 1 hit.
[Graphical view]
SUPFAMiSSF49299. SSF49299. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF50494. SSF50494. 1 hit.
PROSITEiPS50093. PKD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAPI_ACHLY
AccessioniPrimary (citable) accession number: P15636
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: November 2, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.