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Protein

Protease 1

Gene
N/A
Organism
Achromobacter lyticus
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Preferential cleavage: Lys-|-Xaa, including Lys-|-Pro.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei262 – 2621Charge relay systemBy similarity
Active sitei318 – 3181Charge relay systemBy similarity
Active sitei399 – 3991Charge relay systemBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BRENDAi3.4.21.50. 74.

Protein family/group databases

MEROPSiS01.280.

Names & Taxonomyi

Protein namesi
Recommended name:
Protease 1 (EC:3.4.21.50)
Alternative name(s):
API
Lysyl endopeptidase
Protease I
OrganismiAchromobacter lyticus
Taxonomic identifieri224 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeAchromobacter

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Or 27Sequence analysisAdd
BLAST
Propeptidei21 – 2051851 PublicationPRO_0000028515Add
BLAST
Chaini206 – 473268Protease 1PRO_0000028516Add
BLAST
Propeptidei474 – 653180Thr/Ser-richPRO_0000028517Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi211 ↔ 4211 Publication
Disulfide bondi217 ↔ 2851 Publication
Disulfide bondi241 ↔ 2631 Publication

Post-translational modificationi

Three disulfide bonds are present.1 Publication

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PRIDEiP15636.

Structurei

Secondary structure

1
653
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi218 – 2203Combined sources
Helixi226 – 2294Combined sources
Beta strandi230 – 2356Combined sources
Beta strandi238 – 2469Combined sources
Beta strandi256 – 2605Combined sources
Helixi261 – 2633Combined sources
Helixi268 – 2725Combined sources
Beta strandi275 – 2773Combined sources
Helixi291 – 2944Combined sources
Beta strandi304 – 3063Combined sources
Beta strandi308 – 3147Combined sources
Turni315 – 3184Combined sources
Beta strandi319 – 3268Combined sources
Helixi330 – 3323Combined sources
Beta strandi335 – 3373Combined sources
Beta strandi349 – 3535Combined sources
Helixi355 – 3573Combined sources
Beta strandi361 – 3655Combined sources
Beta strandi370 – 3723Combined sources
Beta strandi376 – 3783Combined sources
Beta strandi380 – 3867Combined sources
Beta strandi402 – 4043Combined sources
Beta strandi410 – 4167Combined sources
Helixi425 – 4273Combined sources
Beta strandi428 – 4347Combined sources
Helixi435 – 4406Combined sources
Helixi445 – 4473Combined sources
Helixi450 – 4534Combined sources
Beta strandi461 – 4633Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ARBX-ray1.20A206-473[»]
1ARCX-ray2.00A206-473[»]
4GPGOther1.90A206-473[»]
ProteinModelPortaliP15636.
SMRiP15636. Positions 206-468.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15636.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini474 – 55380PKDPROSITE-ProRule annotationAdd
BLAST

Domaini

The C-terminal extension has little effect on the function of API.

Sequence similaritiesi

Belongs to the peptidase S1 family.Curated
Contains 1 PKD domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

KOiK01337.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.670. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR017323. Lysyl_endo-pept.
IPR009003. Peptidase_S1_PA.
IPR022409. PKD/Chitinase_dom.
IPR000601. PKD_dom.
IPR002884. PrprotnconvertsP.
[Graphical view]
PfamiPF01483. P_proprotein. 1 hit.
PF00801. PKD. 1 hit.
[Graphical view]
PIRSFiPIRSF037930. Lysyl_endopeptidase. 1 hit.
SMARTiSM00089. PKD. 1 hit.
[Graphical view]
SUPFAMiSSF49299. SSF49299. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF50494. SSF50494. 1 hit.
PROSITEiPS50093. PKD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15636-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRICGSLLL LGLSISAALA APASRPAAFD YANLSSVDKV ALRTMPAVDV
60 70 80 90 100
AKAKAEDLQR DKRGDIPRFA LAIDVDMTPQ NSGAWEYTAD GQFAVWRQRV
110 120 130 140 150
RSEKALSLNF GFTDYYMPAG GRLLVYPATQ APAGDRGLIS QYDASNNNSA
160 170 180 190 200
RQLWTAVVPG AEAVIEAVIP RDKVGEFKLR LTKVNHDYVG FGPLARRLAA
210 220 230 240 250
ASGEKGVSGS CNIDVVCPEG DGRRDIIRAV GAYSKSGTLA CTGSLVNNTA
260 270 280 290 300
NDRKMYFLTA HHCGMGTAST AASIVVYWNY QNSTCRAPNT PASGANGDGS
310 320 330 340 350
MSQTQSGSTV KATYATSDFT LLELNNAANP AFNLFWAGWD RRDQNYPGAI
360 370 380 390 400
AIHHPNVAEK RISNSTSPTS FVAWGGGAGT THLNVQWQPS GGVTEPGSSG
410 420 430 440 450
SPIYSPEKRV LGQLHGGPSS CSATGTNRSD QYGRVFTSWT GGGAAASRLS
460 470 480 490 500
DWLDPASTGA QFIDGLDSGG GTPNTPPVAN FTSTTSGLTA TFTDSSTDSD
510 520 530 540 550
GSIASRSWNF GDGSTSTATN PSKTYAAAGT YTVTLTVTDN GGATNTKTGS
560 570 580 590 600
VTVSGGPGAQ TYTNDTDVAI PDNATVESPI TVSGRTGNGS ATTPIQVTIY
610 620 630 640 650
HTYKSDLKVD LVAPDGTVYN LHNRTGGSAH NIIQTFTKDL SSEAAQRAPG

SCG
Length:653
Mass (Da):68,125
Last modified:April 1, 1990 - v1
Checksum:i3A55159247EFFE7C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05128 Genomic DNA. Translation: AAA78946.1.
PIRiA32687.

Genome annotation databases

KEGGiag:AAA78946.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05128 Genomic DNA. Translation: AAA78946.1.
PIRiA32687.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ARBX-ray1.20A206-473[»]
1ARCX-ray2.00A206-473[»]
4GPGOther1.90A206-473[»]
ProteinModelPortaliP15636.
SMRiP15636. Positions 206-468.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS01.280.

Proteomic databases

PRIDEiP15636.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAA78946.

Phylogenomic databases

KOiK01337.

Enzyme and pathway databases

BRENDAi3.4.21.50. 74.

Miscellaneous databases

EvolutionaryTraceiP15636.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.670. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR017323. Lysyl_endo-pept.
IPR009003. Peptidase_S1_PA.
IPR022409. PKD/Chitinase_dom.
IPR000601. PKD_dom.
IPR002884. PrprotnconvertsP.
[Graphical view]
PfamiPF01483. P_proprotein. 1 hit.
PF00801. PKD. 1 hit.
[Graphical view]
PIRSFiPIRSF037930. Lysyl_endopeptidase. 1 hit.
SMARTiSM00089. PKD. 1 hit.
[Graphical view]
SUPFAMiSSF49299. SSF49299. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF50494. SSF50494. 1 hit.
PROSITEiPS50093. PKD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning, nucleotide sequence, and expression of Achromobacter protease I gene."
    Ohara T., Makino K., Shinagawa H., Nakata A., Norioka S., Sakiyama F.
    J. Biol. Chem. 264:20625-20631(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: M497-1.
  2. "The primary structure and structural characteristics of Achromobacter lyticus protease I, a lysine-specific serine protease."
    Tsunasawa S., Masaki T., Hirose M., Soejima M., Sakiyama F.
    J. Biol. Chem. 264:3832-3839(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 206-473, X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 206-473, DISULFIDE BOND.
    Strain: M497-1.

Entry informationi

Entry nameiAPI_ACHLY
AccessioniPrimary (citable) accession number: P15636
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: April 13, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.