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P15625

- SYFA_YEAST

UniProt

P15625 - SYFA_YEAST

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Protein
Phenylalanine--tRNA ligase alpha subunit
Gene
FRS2, YFL022C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. phenylalanine-tRNA ligase activity Source: UniProtKB-EC
  3. protein binding Source: IntAct
  4. tRNA binding Source: InterPro

GO - Biological processi

  1. phenylalanyl-tRNA aminoacylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30438-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylalanine--tRNA ligase alpha subunit (EC:6.1.1.20)
Alternative name(s):
Phenylalanyl-tRNA synthetase alpha subunit
Short name:
PheRS
Gene namesi
Name:FRS2
Ordered Locus Names:YFL022C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VI

Organism-specific databases

SGDiS000001872. FRS2.

Subcellular locationi

GO - Cellular componenti

  1. phenylalanine-tRNA ligase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 503502Phenylalanine--tRNA ligase alpha subunit
PRO_0000126828Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP15625.
PaxDbiP15625.
PeptideAtlasiP15625.

Expressioni

Gene expression databases

GenevestigatoriP15625.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.

Binary interactionsi

WithEntry#Exp.IntActNotes
FRS1P156243EBI-18678,EBI-18684

Protein-protein interaction databases

BioGridi31124. 25 interactions.
DIPiDIP-5428N.
IntActiP15625. 7 interactions.
MINTiMINT-479586.
STRINGi4932.YFL022C.

Structurei

3D structure databases

ProteinModelPortaliP15625.
SMRiP15625. Positions 155-500.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 173172Contains the major tRNA-Phe binding sites
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0016.
GeneTreeiENSGT00390000006387.
HOGENOMiHOG000230294.
KOiK01889.
OMAiHEESFRT.
OrthoDBiEOG7BGHVT.

Family and domain databases

InterProiIPR006195. aa-tRNA-synth_II.
IPR004529. Phe-tRNA-synth_IIc_asu.
IPR002319. Phenylalanyl-tRNA_Synthase.
[Graphical view]
PfamiPF01409. tRNA-synt_2d. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00468. pheS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15625-1 [UniParc]FASTAAdd to Basket

« Hide

MSDFQLEILK KLDELDEIKS TLATFPQHGS QDVLSALNSL KAHNKLEFSK    50
VDTVTYDLTK EGAQILNEGS YEIKLVKLIQ ELGQLQIKDV MSKLGPQVGK 100
VGQARAFKNG WIAKNASNEL ELSAKLQNTD LNELTDETQS ILAQIKNNSH 150
LDSIDAKILN DLKKRKLIAQ GKITDFNVTK GPEFSTDLTK LETDLTSDMV 200
STNAYKDLKF KPYNFNSQGV QISSGALHPL NKVREEFRQI FFSMGFTEMP 250
SNQYVETGFW NFDALYVPQQ HPARDLQDTF YIKDPLTADL PDDKTYMDNI 300
KAVHEQGRFG SIGYRYNWKP EECQKLVLRT HSTAISARML HDLAKDPKPT 350
RLFSIDRVFR NEAVDATHLA EFHQVEGVLA DYNITLGDLI KFMEEFFERM 400
GVTGLRFKPT YNPYTEPSME IFSWHEGLQK WVEIGNSGMF RPEMLESMGL 450
PKDLRVLGWG LSLERPTMIK YKVQNIRELL GHKVSLDFIE TNPAARLDED 500
LYE 503
Length:503
Mass (Da):57,511
Last modified:January 23, 2007 - v3
Checksum:i50B7EAC675AB559B
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti177 – 1771N → S in AAA35152. 1 Publication
Sequence conflicti289 – 2891D → E in AAA35152. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03965 Genomic DNA. Translation: AAA35152.1.
D50617 Genomic DNA. Translation: BAA09216.1.
BK006940 Genomic DNA. Translation: DAA12418.1.
PIRiS56232. YFBYAC.
RefSeqiNP_116631.1. NM_001179944.2.

Genome annotation databases

EnsemblFungiiYFL022C; YFL022C; YFL022C.
GeneIDi850522.
KEGGisce:YFL022C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03965 Genomic DNA. Translation: AAA35152.1 .
D50617 Genomic DNA. Translation: BAA09216.1 .
BK006940 Genomic DNA. Translation: DAA12418.1 .
PIRi S56232. YFBYAC.
RefSeqi NP_116631.1. NM_001179944.2.

3D structure databases

ProteinModelPortali P15625.
SMRi P15625. Positions 155-500.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31124. 25 interactions.
DIPi DIP-5428N.
IntActi P15625. 7 interactions.
MINTi MINT-479586.
STRINGi 4932.YFL022C.

Proteomic databases

MaxQBi P15625.
PaxDbi P15625.
PeptideAtlasi P15625.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YFL022C ; YFL022C ; YFL022C .
GeneIDi 850522.
KEGGi sce:YFL022C.

Organism-specific databases

SGDi S000001872. FRS2.

Phylogenomic databases

eggNOGi COG0016.
GeneTreei ENSGT00390000006387.
HOGENOMi HOG000230294.
KOi K01889.
OMAi HEESFRT.
OrthoDBi EOG7BGHVT.

Enzyme and pathway databases

BioCyci YEAST:G3O-30438-MONOMER.

Miscellaneous databases

NextBioi 966257.
PROi P15625.

Gene expression databases

Genevestigatori P15625.

Family and domain databases

InterProi IPR006195. aa-tRNA-synth_II.
IPR004529. Phe-tRNA-synth_IIc_asu.
IPR002319. Phenylalanyl-tRNA_Synthase.
[Graphical view ]
Pfami PF01409. tRNA-synt_2d. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00468. pheS. 1 hit.
PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and expression of the genes encoding the alpha and beta subunits of yeast phenylalanyl-tRNA synthetase."
    Sanni A., Mirande M., Ebel J.-P., Boulanger Y., Waller J.-P., Fasiolo F.
    J. Biol. Chem. 263:15407-15415(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Identification of the major tRNA(Phe) binding domain in the tetrameric structure of cytoplasmic phenylalanyl-tRNA synthetase from baker's yeast."
    Fasiolo F., Sanni A., Potier S., Ebel J.-P., Boulanger Y.
    FEBS Lett. 242:351-356(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE, DOMAIN TRNA-BINDING.
  5. "Proteome studies of Saccharomyces cerevisiae: identification and characterization of abundant proteins."
    Garrels J.I., McLaughlin C.S., Warner J.R., Futcher B., Latter G.I., Kobayashi R., Schwender B., Volpe T., Anderson D.S., Mesquita-Fuentes R., Payne W.E.
    Electrophoresis 18:1347-1360(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION AT SER-2.

Entry informationi

Entry nameiSYFA_YEAST
AccessioniPrimary (citable) accession number: P15625
Secondary accession number(s): D6VTK8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 143 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Caution

Was originally (1 Publication) erroneously assigned as a beta subunit.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

External Data

Dasty 3

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