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Protein

Phenylalanine--tRNA ligase alpha subunit

Gene

FRS2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Caution

Was originally erroneously assigned as a beta subunit.1 Publication

Catalytic activityi

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).

GO - Molecular functioni

  • aminoacyl-tRNA editing activity Source: SGD
  • ATP binding Source: UniProtKB-KW
  • phenylalanine-tRNA ligase activity Source: UniProtKB-EC
  • tRNA binding Source: InterPro

GO - Biological processi

  • phenylalanyl-tRNA aminoacylation Source: SGD

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30438-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylalanine--tRNA ligase alpha subunit (EC:6.1.1.20)
Alternative name(s):
Phenylalanyl-tRNA synthetase alpha subunit
Short name:
PheRS
Gene namesi
Name:FRS2
Ordered Locus Names:YFL022C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFL022C
SGDiS000001872 FRS2

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001268282 – 503Phenylalanine--tRNA ligase alpha subunitAdd BLAST502

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserine1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP15625
PaxDbiP15625
PRIDEiP15625
TopDownProteomicsiP15625

PTM databases

iPTMnetiP15625

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.

Binary interactionsi

WithEntry#Exp.IntActNotes
FRS1P156242EBI-18678,EBI-18684

Protein-protein interaction databases

BioGridi31124, 102 interactors
DIPiDIP-5428N
IntActiP15625, 11 interactors
MINTiP15625
STRINGi4932.YFL022C

Structurei

3D structure databases

ProteinModelPortaliP15625
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 173Contains the major tRNA-Phe binding sitesAdd BLAST172

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000006387
HOGENOMiHOG000230294
InParanoidiP15625
KOiK01889
OMAiRFKPAFN
OrthoDBiEOG092C1U6R

Family and domain databases

InterProiView protein in InterPro
IPR006195 aa-tRNA-synth_II
IPR004529 Phe-tRNA-synth_IIc_asu
IPR002319 Phenylalanyl-tRNA_Synthase
PfamiView protein in Pfam
PF01409 tRNA-synt_2d, 1 hit
TIGRFAMsiTIGR00468 pheS, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15625-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDFQLEILK KLDELDEIKS TLATFPQHGS QDVLSALNSL KAHNKLEFSK
60 70 80 90 100
VDTVTYDLTK EGAQILNEGS YEIKLVKLIQ ELGQLQIKDV MSKLGPQVGK
110 120 130 140 150
VGQARAFKNG WIAKNASNEL ELSAKLQNTD LNELTDETQS ILAQIKNNSH
160 170 180 190 200
LDSIDAKILN DLKKRKLIAQ GKITDFNVTK GPEFSTDLTK LETDLTSDMV
210 220 230 240 250
STNAYKDLKF KPYNFNSQGV QISSGALHPL NKVREEFRQI FFSMGFTEMP
260 270 280 290 300
SNQYVETGFW NFDALYVPQQ HPARDLQDTF YIKDPLTADL PDDKTYMDNI
310 320 330 340 350
KAVHEQGRFG SIGYRYNWKP EECQKLVLRT HSTAISARML HDLAKDPKPT
360 370 380 390 400
RLFSIDRVFR NEAVDATHLA EFHQVEGVLA DYNITLGDLI KFMEEFFERM
410 420 430 440 450
GVTGLRFKPT YNPYTEPSME IFSWHEGLQK WVEIGNSGMF RPEMLESMGL
460 470 480 490 500
PKDLRVLGWG LSLERPTMIK YKVQNIRELL GHKVSLDFIE TNPAARLDED

LYE
Length:503
Mass (Da):57,511
Last modified:January 23, 2007 - v3
Checksum:i50B7EAC675AB559B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti177N → S in AAA35152 (PubMed:3049607).Curated1
Sequence conflicti289D → E in AAA35152 (PubMed:3049607).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03965 Genomic DNA Translation: AAA35152.1
D50617 Genomic DNA Translation: BAA09216.1
BK006940 Genomic DNA Translation: DAA12418.1
PIRiS56232 YFBYAC
RefSeqiNP_116631.1, NM_001179944.2

Genome annotation databases

EnsemblFungiiBAA09216; BAA09216; BAA09216
YFL022C; YFL022C; YFL022C
GeneIDi850522
KEGGisce:YFL022C

Similar proteinsi

Entry informationi

Entry nameiSYFA_YEAST
AccessioniPrimary (citable) accession number: P15625
Secondary accession number(s): D6VTK8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 175 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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