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P15625 (SYFA_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 141. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phenylalanine--tRNA ligase alpha subunit

EC=6.1.1.20
Alternative name(s):
Phenylalanyl-tRNA synthetase alpha subunit
Short name=PheRS
Gene names
Name:FRS2
Ordered Locus Names:YFL022C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length503 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).

Subunit structure

Tetramer of two alpha and two beta subunits.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 2 subfamily.

Caution

Was originally (Ref.1) erroneously assigned as a beta subunit.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

FRS1P156243EBI-18678,EBI-18684

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 503502Phenylalanine--tRNA ligase alpha subunit
PRO_0000126828

Regions

Region2 – 173172Contains the major tRNA-Phe binding sites

Amino acid modifications

Modified residue21N-acetylserine Ref.5

Experimental info

Sequence conflict1771N → S in AAA35152. Ref.1
Sequence conflict2891D → E in AAA35152. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P15625 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 50B7EAC675AB559B

FASTA50357,511
        10         20         30         40         50         60 
MSDFQLEILK KLDELDEIKS TLATFPQHGS QDVLSALNSL KAHNKLEFSK VDTVTYDLTK 

        70         80         90        100        110        120 
EGAQILNEGS YEIKLVKLIQ ELGQLQIKDV MSKLGPQVGK VGQARAFKNG WIAKNASNEL 

       130        140        150        160        170        180 
ELSAKLQNTD LNELTDETQS ILAQIKNNSH LDSIDAKILN DLKKRKLIAQ GKITDFNVTK 

       190        200        210        220        230        240 
GPEFSTDLTK LETDLTSDMV STNAYKDLKF KPYNFNSQGV QISSGALHPL NKVREEFRQI 

       250        260        270        280        290        300 
FFSMGFTEMP SNQYVETGFW NFDALYVPQQ HPARDLQDTF YIKDPLTADL PDDKTYMDNI 

       310        320        330        340        350        360 
KAVHEQGRFG SIGYRYNWKP EECQKLVLRT HSTAISARML HDLAKDPKPT RLFSIDRVFR 

       370        380        390        400        410        420 
NEAVDATHLA EFHQVEGVLA DYNITLGDLI KFMEEFFERM GVTGLRFKPT YNPYTEPSME 

       430        440        450        460        470        480 
IFSWHEGLQK WVEIGNSGMF RPEMLESMGL PKDLRVLGWG LSLERPTMIK YKVQNIRELL 

       490        500 
GHKVSLDFIE TNPAARLDED LYE 

« Hide

References

« Hide 'large scale' references
[1]"Structure and expression of the genes encoding the alpha and beta subunits of yeast phenylalanyl-tRNA synthetase."
Sanni A., Mirande M., Ebel J.-P., Boulanger Y., Waller J.-P., Fasiolo F.
J. Biol. Chem. 263:15407-15415(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae."
Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T.
Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Identification of the major tRNA(Phe) binding domain in the tetrameric structure of cytoplasmic phenylalanyl-tRNA synthetase from baker's yeast."
Fasiolo F., Sanni A., Potier S., Ebel J.-P., Boulanger Y.
FEBS Lett. 242:351-356(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE, DOMAIN TRNA-BINDING.
[5]"Proteome studies of Saccharomyces cerevisiae: identification and characterization of abundant proteins."
Garrels J.I., McLaughlin C.S., Warner J.R., Futcher B., Latter G.I., Kobayashi R., Schwender B., Volpe T., Anderson D.S., Mesquita-Fuentes R., Payne W.E.
Electrophoresis 18:1347-1360(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION AT SER-2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J03965 Genomic DNA. Translation: AAA35152.1.
D50617 Genomic DNA. Translation: BAA09216.1.
BK006940 Genomic DNA. Translation: DAA12418.1.
PIRYFBYAC. S56232.
RefSeqNP_116631.1. NM_001179944.2.

3D structure databases

ProteinModelPortalP15625.
SMRP15625. Positions 1-501.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31124. 25 interactions.
DIPDIP-5428N.
IntActP15625. 7 interactions.
MINTMINT-479586.
STRING4932.YFL022C.

Proteomic databases

PaxDbP15625.
PeptideAtlasP15625.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYFL022C; YFL022C; YFL022C.
GeneID850522.
KEGGsce:YFL022C.

Organism-specific databases

SGDS000001872. FRS2.

Phylogenomic databases

eggNOGCOG0016.
GeneTreeENSGT00390000006387.
HOGENOMHOG000230294.
KOK01889.
OMAFGFHTGL.
OrthoDBEOG7BGHVT.

Enzyme and pathway databases

BioCycYEAST:G3O-30438-MONOMER.

Gene expression databases

GenevestigatorP15625.

Family and domain databases

InterProIPR006195. aa-tRNA-synth_II.
IPR004529. Phe-tRNA-synth_IIc_asu.
IPR002319. Phenylalanyl-tRNA_Synthase.
[Graphical view]
PfamPF01409. tRNA-synt_2d. 1 hit.
[Graphical view]
TIGRFAMsTIGR00468. pheS. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio966257.
PROP15625.

Entry information

Entry nameSYFA_YEAST
AccessionPrimary (citable) accession number: P15625
Secondary accession number(s): D6VTK8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 23, 2007
Last modified: March 19, 2014
This is version 141 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries