P15624 (SYFB_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase beta subunit
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase beta subunit
Short name=PheRS

Gene names

Name:	FRS1
Ordered Locus Names:	YLR060W
ORF Names:	L2165

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	595 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).
Subunit structure	Tetramer of two alpha and two beta subunits.
Subcellular location	Cytoplasm.
Miscellaneous	Present with 5440 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 2 subfamily. Contains 1 B5 domain.
Caution	Was originally (Ref.1) erroneously assigned as an alpha subunit.
Sequence caution	The sequence AAA35151.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	phenylalanyl-tRNA aminoacylation Traceable author statement Ref.1. Source: SGD
Cellular_component	phenylalanine-tRNA ligase complex Inferred from direct assay Ref.1. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: InterPro phenylalanine-tRNA ligase activity Inferred from mutant phenotype Ref.1. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
FRS2	P15625	3	EBI-18684,EBI-18678

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 595	594	Phenylalanine--tRNA ligase beta subunit		PRO_0000127023
Regions
	Domain	292 – 370	79	B5
	Region	86 – 90	5	CCA binding of tRNA Potential
Amino acid modifications
	Modified residue	124	1	Phosphoserine Ref.7
	Modified residue	375	1	Phosphoserine Ref.7
	Modified residue	437	1	Phosphothreonine Ref.6
Experimental info
	Sequence conflict	18	1	N → D AA sequence Ref.1
	Sequence conflict	426	1	G → D in AAA35151. Ref.1
	Sequence conflict	544	1	F → L in AAT92797. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P15624 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 68DA23E49644F801	FASTA	595	67,365
	10 20 30 40 50 60 MPTVSVNKQQ LFDLLGKNYT SQEFDELCFE FGMEMDEDTT EEALKTGEEP ELKLDISANR 70 80 90 100 110 120 YDLLCIEGIS QSLNEYLERK ERPDYKLSKP TTKLIIDKST EQIRPFATAA VLRNIKLNEK 130 140 150 160 170 180 SYASFIALQD KLHANLCRNR SLVAMGTHDL DSIEGPFHYR ALPPKDIKFV PLNQTQEFTG 190 200 210 220 230 240 DKLIEFYKSP EQKNNIGRYV HIIEDSPVFP VIMDSKDRVC SLPPLINSEH SKISVNTRNI 250 260 270 280 290 300 LIDITATDKT KAEIVLNILT TMFSRYCDEP FTVEPVEIVS EHNGQSRLAP NFNDRIMDVS 310 320 330 340 350 360 IKYINSCLGL DQSADEIAHC LKKMSLHAVQ SKEDKDILHV DIPVTRPDIL HACDIMEDAA 370 380 390 400 410 420 VGYGFNNLPK GEKLSNANFI AKPLPINKVS DIFRVASSQA TWVEVLPLTL CSHDENFKFL 430 440 450 460 470 480 RQSDNGDLAV KLANPKTLEY QVVRTTLLPG ILKTVKENRK HSLPIKVFET GDVVFKDDKL 490 500 510 520 530 540 ERKAYNERHW AAIYVGKNSG FEIIQGLLGK IMQTFRTEWI ADYGAAASGR GYWIEEDDSV 550 560 570 580 590 KTYFPGRGAK VMFRSKEGAE PKQIGHLGVL HPEVMMNFDV PFAASFVEVN AEVFL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and expression of the genes encoding the alpha and beta subunits of yeast phenylalanyl-tRNA synthetase." Sanni A., Mirande M., Ebel J.-P., Boulanger Y., Waller J.-P., Fasiolo F. J. Biol. Chem. 263:15407-15415(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-437, MASS SPECTROMETRY.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-124 AND SER-375, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J03964 Genomic DNA. Translation: AAA35151.1. Different initiation. X94607 Genomic DNA. Translation: CAA64307.1. Z73232 Genomic DNA. Translation: CAA97591.1. AY692778 Genomic DNA. Translation: AAT92797.1. BK006945 Genomic DNA. Translation: DAA09378.1.
PIR	YFBYBC. S61634.
RefSeq	NP_013161.1. NM_001181947.1.
3D structure databases
ProteinModelPortal	P15624.
SMR	P15624. Positions 1-595.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6746N.
IntAct	P15624. 3 interactions.
MINT	MINT-616734.
STRING	4932.YLR060W.
Proteomic databases
PaxDb	P15624.
PeptideAtlas	P15624.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR060W; YLR060W; YLR060W.
GeneID	850749.
KEGG	sce:YLR060W.
Organism-specific databases
SGD	S000004050. FRS1.
Phylogenomic databases
eggNOG	COG0072.
GeneTree	ENSGT00530000063489.
HOGENOM	HOG000105095.
KO	K01890.
OMA	FFPNRPD.
OrthoDB	EOG46HKJZ.
Gene expression databases
Genevestigator	P15624.
GermOnline	YLR060W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.30.56.20. 1 hit.
InterPro	IPR005146. B3/B4_tRNA-bd. IPR009061. DNA-bd_dom_put. IPR004531. Phe-tRNA-synth_IIc_bsu_arc. IPR005147. tRNA_synthase_B5-dom. [Graphical view]
Pfam	PF03483. B3_4. 1 hit. PF03484. B5. 1 hit. [Graphical view]
SMART	SM00873. B3_4. 1 hit. SM00874. B5. 1 hit. [Graphical view]
SUPFAM	SSF46955. Putativ_DNA_bind. 2 hits.
TIGRFAMs	TIGR00471. pheT_arch. 1 hit.
PROSITE	PS51483. B5. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	966879.

Entry information

Entry name

SYFB_YEAST

Accession

Primary (citable) accession number: P15624
Secondary accession number(s): D6VY62, E9P8X0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 124 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program