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Protein

Phenylalanine--tRNA ligase beta subunit

Gene

FRS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 5440 molecules/cell in log phase SD medium.1 Publication

Caution

Was originally erroneously assigned as an alpha subunit.1 Publication

Catalytic activityi

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).

GO - Molecular functioni

GO - Biological processi

  • phenylalanyl-tRNA aminoacylation Source: SGD

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32214-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylalanine--tRNA ligase beta subunit (EC:6.1.1.20)
Alternative name(s):
Phenylalanyl-tRNA synthetase beta subunit
Short name:
PheRS
Gene namesi
Name:FRS1
Ordered Locus Names:YLR060W
ORF Names:L2165
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR060W
SGDiS000004050 FRS1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001270232 – 595Phenylalanine--tRNA ligase beta subunitAdd BLAST594

Proteomic databases

MaxQBiP15624
PaxDbiP15624
PRIDEiP15624

PTM databases

CarbonylDBiP15624
iPTMnetiP15624

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi31335229 interactors.
DIPiDIP-6746N
IntActiP15624 17 interactors.
MINTiP15624
STRINGi4932.YLR060W

Structurei

3D structure databases

ProteinModelPortaliP15624
SMRiP15624
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini292 – 370B5PROSITE-ProRule annotationAdd BLAST79

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni86 – 90CCA binding of tRNASequence analysis5

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00530000063489
HOGENOMiHOG000105095
InParanoidiP15624
KOiK01890
OMAiTFTLCSR
OrthoDBiEOG092C0WBD

Family and domain databases

Gene3Di3.50.40.101 hit
InterProiView protein in InterPro
IPR005146 B3/B4_tRNA-bd
IPR009061 DNA-bd_dom_put_sf
IPR004531 Phe-tRNA-synth_IIc_bsu_arc
IPR020825 Phe-tRNA_synthase_B3/B4
IPR005147 tRNA_synthase_B5-dom
PfamiView protein in Pfam
PF03483 B3_4, 1 hit
PF03484 B5, 1 hit
SMARTiView protein in SMART
SM00873 B3_4, 1 hit
SM00874 B5, 1 hit
SUPFAMiSSF46955 SSF46955, 2 hits
TIGRFAMsiTIGR00471 pheT_arch, 1 hit
PROSITEiView protein in PROSITE
PS51483 B5, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15624-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTVSVNKQQ LFDLLGKNYT SQEFDELCFE FGMEMDEDTT EEALKTGEEP
60 70 80 90 100
ELKLDISANR YDLLCIEGIS QSLNEYLERK ERPDYKLSKP TTKLIIDKST
110 120 130 140 150
EQIRPFATAA VLRNIKLNEK SYASFIALQD KLHANLCRNR SLVAMGTHDL
160 170 180 190 200
DSIEGPFHYR ALPPKDIKFV PLNQTQEFTG DKLIEFYKSP EQKNNIGRYV
210 220 230 240 250
HIIEDSPVFP VIMDSKDRVC SLPPLINSEH SKISVNTRNI LIDITATDKT
260 270 280 290 300
KAEIVLNILT TMFSRYCDEP FTVEPVEIVS EHNGQSRLAP NFNDRIMDVS
310 320 330 340 350
IKYINSCLGL DQSADEIAHC LKKMSLHAVQ SKEDKDILHV DIPVTRPDIL
360 370 380 390 400
HACDIMEDAA VGYGFNNLPK GEKLSNANFI AKPLPINKVS DIFRVASSQA
410 420 430 440 450
TWVEVLPLTL CSHDENFKFL RQSDNGDLAV KLANPKTLEY QVVRTTLLPG
460 470 480 490 500
ILKTVKENRK HSLPIKVFET GDVVFKDDKL ERKAYNERHW AAIYVGKNSG
510 520 530 540 550
FEIIQGLLGK IMQTFRTEWI ADYGAAASGR GYWIEEDDSV KTYFPGRGAK
560 570 580 590
VMFRSKEGAE PKQIGHLGVL HPEVMMNFDV PFAASFVEVN AEVFL
Length:595
Mass (Da):67,365
Last modified:January 23, 2007 - v3
Checksum:i68DA23E49644F801
GO

Sequence cautioni

The sequence AAA35151 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18N → D AA sequence (PubMed:3049607).Curated1
Sequence conflicti426G → D in AAA35151 (PubMed:3049607).Curated1
Sequence conflicti544F → L in AAT92797 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03964 Genomic DNA Translation: AAA35151.1 Different initiation.
X94607 Genomic DNA Translation: CAA64307.1
Z73232 Genomic DNA Translation: CAA97591.1
AY692778 Genomic DNA Translation: AAT92797.1
BK006945 Genomic DNA Translation: DAA09378.1
PIRiS61634 YFBYBC
RefSeqiNP_013161.1, NM_001181947.1

Genome annotation databases

EnsemblFungiiYLR060W; YLR060W; YLR060W
GeneIDi850749
KEGGisce:YLR060W

Similar proteinsi

Entry informationi

Entry nameiSYFB_YEAST
AccessioniPrimary (citable) accession number: P15624
Secondary accession number(s): D6VY62, E9P8X0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 162 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome