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P15624

- SYFB_YEAST

UniProt

P15624 - SYFB_YEAST

Protein

Phenylalanine--tRNA ligase beta subunit

Gene

FRS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. phenylalanine-tRNA ligase activity Source: UniProtKB-EC
    4. protein binding Source: IntAct
    5. RNA binding Source: InterPro

    GO - Biological processi

    1. phenylalanyl-tRNA aminoacylation Source: SGD

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32214-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phenylalanine--tRNA ligase beta subunit (EC:6.1.1.20)
    Alternative name(s):
    Phenylalanyl-tRNA synthetase beta subunit
    Short name:
    PheRS
    Gene namesi
    Name:FRS1
    Ordered Locus Names:YLR060W
    ORF Names:L2165
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    SGDiS000004050. FRS1.

    Subcellular locationi

    GO - Cellular componenti

    1. phenylalanine-tRNA ligase complex Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 595594Phenylalanine--tRNA ligase beta subunitPRO_0000127023Add
    BLAST

    Proteomic databases

    MaxQBiP15624.
    PaxDbiP15624.
    PeptideAtlasiP15624.

    Expressioni

    Gene expression databases

    GenevestigatoriP15624.

    Interactioni

    Subunit structurei

    Tetramer of two alpha and two beta subunits.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    FRS2P156253EBI-18684,EBI-18678

    Protein-protein interaction databases

    BioGridi31335. 26 interactions.
    DIPiDIP-6746N.
    IntActiP15624. 5 interactions.
    MINTiMINT-616734.
    STRINGi4932.YLR060W.

    Structurei

    3D structure databases

    ProteinModelPortaliP15624.
    SMRiP15624. Positions 1-595.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini292 – 37079B5PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni86 – 905CCA binding of tRNASequence Analysis

    Sequence similaritiesi

    Contains 1 B5 domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0072.
    GeneTreeiENSGT00530000063489.
    HOGENOMiHOG000105095.
    KOiK01890.
    OMAiEVMPLIL.
    OrthoDBiEOG77M8XG.

    Family and domain databases

    Gene3Di3.30.56.20. 1 hit.
    InterProiIPR005146. B3/B4_tRNA-bd.
    IPR009061. DNA-bd_dom_put.
    IPR004531. Phe-tRNA-synth_IIc_bsu_arc.
    IPR005147. tRNA_synthase_B5-dom.
    [Graphical view]
    PfamiPF03483. B3_4. 1 hit.
    PF03484. B5. 1 hit.
    [Graphical view]
    SMARTiSM00873. B3_4. 1 hit.
    SM00874. B5. 1 hit.
    [Graphical view]
    SUPFAMiSSF46955. SSF46955. 2 hits.
    TIGRFAMsiTIGR00471. pheT_arch. 1 hit.
    PROSITEiPS51483. B5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P15624-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPTVSVNKQQ LFDLLGKNYT SQEFDELCFE FGMEMDEDTT EEALKTGEEP    50
    ELKLDISANR YDLLCIEGIS QSLNEYLERK ERPDYKLSKP TTKLIIDKST 100
    EQIRPFATAA VLRNIKLNEK SYASFIALQD KLHANLCRNR SLVAMGTHDL 150
    DSIEGPFHYR ALPPKDIKFV PLNQTQEFTG DKLIEFYKSP EQKNNIGRYV 200
    HIIEDSPVFP VIMDSKDRVC SLPPLINSEH SKISVNTRNI LIDITATDKT 250
    KAEIVLNILT TMFSRYCDEP FTVEPVEIVS EHNGQSRLAP NFNDRIMDVS 300
    IKYINSCLGL DQSADEIAHC LKKMSLHAVQ SKEDKDILHV DIPVTRPDIL 350
    HACDIMEDAA VGYGFNNLPK GEKLSNANFI AKPLPINKVS DIFRVASSQA 400
    TWVEVLPLTL CSHDENFKFL RQSDNGDLAV KLANPKTLEY QVVRTTLLPG 450
    ILKTVKENRK HSLPIKVFET GDVVFKDDKL ERKAYNERHW AAIYVGKNSG 500
    FEIIQGLLGK IMQTFRTEWI ADYGAAASGR GYWIEEDDSV KTYFPGRGAK 550
    VMFRSKEGAE PKQIGHLGVL HPEVMMNFDV PFAASFVEVN AEVFL 595
    Length:595
    Mass (Da):67,365
    Last modified:January 23, 2007 - v3
    Checksum:i68DA23E49644F801
    GO

    Sequence cautioni

    The sequence AAA35151.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti18 – 181N → D AA sequence (PubMed:3049607)Curated
    Sequence conflicti426 – 4261G → D in AAA35151. (PubMed:3049607)Curated
    Sequence conflicti544 – 5441F → L in AAT92797. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03964 Genomic DNA. Translation: AAA35151.1. Different initiation.
    X94607 Genomic DNA. Translation: CAA64307.1.
    Z73232 Genomic DNA. Translation: CAA97591.1.
    AY692778 Genomic DNA. Translation: AAT92797.1.
    BK006945 Genomic DNA. Translation: DAA09378.1.
    PIRiS61634. YFBYBC.
    RefSeqiNP_013161.1. NM_001181947.1.

    Genome annotation databases

    EnsemblFungiiYLR060W; YLR060W; YLR060W.
    GeneIDi850749.
    KEGGisce:YLR060W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03964 Genomic DNA. Translation: AAA35151.1 . Different initiation.
    X94607 Genomic DNA. Translation: CAA64307.1 .
    Z73232 Genomic DNA. Translation: CAA97591.1 .
    AY692778 Genomic DNA. Translation: AAT92797.1 .
    BK006945 Genomic DNA. Translation: DAA09378.1 .
    PIRi S61634. YFBYBC.
    RefSeqi NP_013161.1. NM_001181947.1.

    3D structure databases

    ProteinModelPortali P15624.
    SMRi P15624. Positions 1-595.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31335. 26 interactions.
    DIPi DIP-6746N.
    IntActi P15624. 5 interactions.
    MINTi MINT-616734.
    STRINGi 4932.YLR060W.

    Proteomic databases

    MaxQBi P15624.
    PaxDbi P15624.
    PeptideAtlasi P15624.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR060W ; YLR060W ; YLR060W .
    GeneIDi 850749.
    KEGGi sce:YLR060W.

    Organism-specific databases

    SGDi S000004050. FRS1.

    Phylogenomic databases

    eggNOGi COG0072.
    GeneTreei ENSGT00530000063489.
    HOGENOMi HOG000105095.
    KOi K01890.
    OMAi EVMPLIL.
    OrthoDBi EOG77M8XG.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32214-MONOMER.

    Miscellaneous databases

    NextBioi 966879.
    PROi P15624.

    Gene expression databases

    Genevestigatori P15624.

    Family and domain databases

    Gene3Di 3.30.56.20. 1 hit.
    InterProi IPR005146. B3/B4_tRNA-bd.
    IPR009061. DNA-bd_dom_put.
    IPR004531. Phe-tRNA-synth_IIc_bsu_arc.
    IPR005147. tRNA_synthase_B5-dom.
    [Graphical view ]
    Pfami PF03483. B3_4. 1 hit.
    PF03484. B5. 1 hit.
    [Graphical view ]
    SMARTi SM00873. B3_4. 1 hit.
    SM00874. B5. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46955. SSF46955. 2 hits.
    TIGRFAMsi TIGR00471. pheT_arch. 1 hit.
    PROSITEi PS51483. B5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure and expression of the genes encoding the alpha and beta subunits of yeast phenylalanyl-tRNA synthetase."
      Sanni A., Mirande M., Ebel J.-P., Boulanger Y., Waller J.-P., Fasiolo F.
      J. Biol. Chem. 263:15407-15415(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Phenylalanyl-tRNA synthetase from baker's yeast. Repeated sequences in the two subunits."
      Robbe-Saul S., Fasiolo F., Boulanger Y.
      FEBS Lett. 84:57-62(1977) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF N-TERMINUS, CLEAVAGE OF INITIATOR METHIONINE.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSYFB_YEAST
    AccessioniPrimary (citable) accession number: P15624
    Secondary accession number(s): D6VY62, E9P8X0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 134 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 5440 molecules/cell in log phase SD medium.1 Publication

    Caution

    Was originally erroneously assigned as an alpha subunit.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3