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P15624 (SYFB_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 133. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phenylalanine--tRNA ligase beta subunit

EC=6.1.1.20
Alternative name(s):
Phenylalanyl-tRNA synthetase beta subunit
Short name=PheRS
Gene names
Name:FRS1
Ordered Locus Names:YLR060W
ORF Names:L2165
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length595 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).

Subunit structure

Tetramer of two alpha and two beta subunits.

Subcellular location

Cytoplasm.

Miscellaneous

Present with 5440 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 2 subfamily.

Contains 1 B5 domain.

Caution

Was originally (Ref.1) erroneously assigned as an alpha subunit.

Sequence caution

The sequence AAA35151.1 differs from that shown. Reason: Erroneous initiation.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

FRS2P156253EBI-18684,EBI-18678

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.5
Chain2 – 595594Phenylalanine--tRNA ligase beta subunit
PRO_0000127023

Regions

Domain292 – 37079B5
Region86 – 905CCA binding of tRNA Potential

Experimental info

Sequence conflict181N → D AA sequence Ref.1
Sequence conflict4261G → D in AAA35151. Ref.1
Sequence conflict5441F → L in AAT92797. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P15624 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 68DA23E49644F801

FASTA59567,365
        10         20         30         40         50         60 
MPTVSVNKQQ LFDLLGKNYT SQEFDELCFE FGMEMDEDTT EEALKTGEEP ELKLDISANR 

        70         80         90        100        110        120 
YDLLCIEGIS QSLNEYLERK ERPDYKLSKP TTKLIIDKST EQIRPFATAA VLRNIKLNEK 

       130        140        150        160        170        180 
SYASFIALQD KLHANLCRNR SLVAMGTHDL DSIEGPFHYR ALPPKDIKFV PLNQTQEFTG 

       190        200        210        220        230        240 
DKLIEFYKSP EQKNNIGRYV HIIEDSPVFP VIMDSKDRVC SLPPLINSEH SKISVNTRNI 

       250        260        270        280        290        300 
LIDITATDKT KAEIVLNILT TMFSRYCDEP FTVEPVEIVS EHNGQSRLAP NFNDRIMDVS 

       310        320        330        340        350        360 
IKYINSCLGL DQSADEIAHC LKKMSLHAVQ SKEDKDILHV DIPVTRPDIL HACDIMEDAA 

       370        380        390        400        410        420 
VGYGFNNLPK GEKLSNANFI AKPLPINKVS DIFRVASSQA TWVEVLPLTL CSHDENFKFL 

       430        440        450        460        470        480 
RQSDNGDLAV KLANPKTLEY QVVRTTLLPG ILKTVKENRK HSLPIKVFET GDVVFKDDKL 

       490        500        510        520        530        540 
ERKAYNERHW AAIYVGKNSG FEIIQGLLGK IMQTFRTEWI ADYGAAASGR GYWIEEDDSV 

       550        560        570        580        590 
KTYFPGRGAK VMFRSKEGAE PKQIGHLGVL HPEVMMNFDV PFAASFVEVN AEVFL 

« Hide

References

« Hide 'large scale' references
[1]"Structure and expression of the genes encoding the alpha and beta subunits of yeast phenylalanyl-tRNA synthetase."
Sanni A., Mirande M., Ebel J.-P., Boulanger Y., Waller J.-P., Fasiolo F.
J. Biol. Chem. 263:15407-15415(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Phenylalanyl-tRNA synthetase from baker's yeast. Repeated sequences in the two subunits."
Robbe-Saul S., Fasiolo F., Boulanger Y.
FEBS Lett. 84:57-62(1977) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF N-TERMINUS, CLEAVAGE OF INITIATOR METHIONINE.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J03964 Genomic DNA. Translation: AAA35151.1. Different initiation.
X94607 Genomic DNA. Translation: CAA64307.1.
Z73232 Genomic DNA. Translation: CAA97591.1.
AY692778 Genomic DNA. Translation: AAT92797.1.
BK006945 Genomic DNA. Translation: DAA09378.1.
PIRYFBYBC. S61634.
RefSeqNP_013161.1. NM_001181947.1.

3D structure databases

ProteinModelPortalP15624.
SMRP15624. Positions 1-595.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31335. 26 interactions.
DIPDIP-6746N.
IntActP15624. 5 interactions.
MINTMINT-616734.
STRING4932.YLR060W.

Proteomic databases

MaxQBP15624.
PaxDbP15624.
PeptideAtlasP15624.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYLR060W; YLR060W; YLR060W.
GeneID850749.
KEGGsce:YLR060W.

Organism-specific databases

SGDS000004050. FRS1.

Phylogenomic databases

eggNOGCOG0072.
GeneTreeENSGT00530000063489.
HOGENOMHOG000105095.
KOK01890.
OMAEVMPLIL.
OrthoDBEOG77M8XG.

Enzyme and pathway databases

BioCycYEAST:G3O-32214-MONOMER.

Gene expression databases

GenevestigatorP15624.

Family and domain databases

Gene3D3.30.56.20. 1 hit.
InterProIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR004531. Phe-tRNA-synth_IIc_bsu_arc.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
PfamPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
[Graphical view]
SMARTSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
[Graphical view]
SUPFAMSSF46955. SSF46955. 2 hits.
TIGRFAMsTIGR00471. pheT_arch. 1 hit.
PROSITEPS51483. B5. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio966879.
PROP15624.

Entry information

Entry nameSYFB_YEAST
AccessionPrimary (citable) accession number: P15624
Secondary accession number(s): D6VY62, E9P8X0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries