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P15624

- SYFB_YEAST

UniProt

P15624 - SYFB_YEAST

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Protein

Phenylalanine--tRNA ligase beta subunit

Gene
FRS1, YLR060W, L2165
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. phenylalanine-tRNA ligase activity Source: UniProtKB-EC
  4. protein binding Source: IntAct
  5. RNA binding Source: InterPro

GO - Biological processi

  1. phenylalanyl-tRNA aminoacylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32214-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylalanine--tRNA ligase beta subunit (EC:6.1.1.20)
Alternative name(s):
Phenylalanyl-tRNA synthetase beta subunit
Short name:
PheRS
Gene namesi
Name:FRS1
Ordered Locus Names:YLR060W
ORF Names:L2165
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XII

Organism-specific databases

SGDiS000004050. FRS1.

Subcellular locationi

GO - Cellular componenti

  1. phenylalanine-tRNA ligase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 595594Phenylalanine--tRNA ligase beta subunitPRO_0000127023Add
BLAST

Proteomic databases

MaxQBiP15624.
PaxDbiP15624.
PeptideAtlasiP15624.

Expressioni

Gene expression databases

GenevestigatoriP15624.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.

Binary interactionsi

WithEntry#Exp.IntActNotes
FRS2P156253EBI-18684,EBI-18678

Protein-protein interaction databases

BioGridi31335. 26 interactions.
DIPiDIP-6746N.
IntActiP15624. 5 interactions.
MINTiMINT-616734.
STRINGi4932.YLR060W.

Structurei

3D structure databases

ProteinModelPortaliP15624.
SMRiP15624. Positions 1-595.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini292 – 37079B5Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni86 – 905CCA binding of tRNA Reviewed prediction

Sequence similaritiesi

Contains 1 B5 domain.

Phylogenomic databases

eggNOGiCOG0072.
GeneTreeiENSGT00530000063489.
HOGENOMiHOG000105095.
KOiK01890.
OMAiEVMPLIL.
OrthoDBiEOG77M8XG.

Family and domain databases

Gene3Di3.30.56.20. 1 hit.
InterProiIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR004531. Phe-tRNA-synth_IIc_bsu_arc.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
PfamiPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
[Graphical view]
SMARTiSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 2 hits.
TIGRFAMsiTIGR00471. pheT_arch. 1 hit.
PROSITEiPS51483. B5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15624-1 [UniParc]FASTAAdd to Basket

« Hide

MPTVSVNKQQ LFDLLGKNYT SQEFDELCFE FGMEMDEDTT EEALKTGEEP    50
ELKLDISANR YDLLCIEGIS QSLNEYLERK ERPDYKLSKP TTKLIIDKST 100
EQIRPFATAA VLRNIKLNEK SYASFIALQD KLHANLCRNR SLVAMGTHDL 150
DSIEGPFHYR ALPPKDIKFV PLNQTQEFTG DKLIEFYKSP EQKNNIGRYV 200
HIIEDSPVFP VIMDSKDRVC SLPPLINSEH SKISVNTRNI LIDITATDKT 250
KAEIVLNILT TMFSRYCDEP FTVEPVEIVS EHNGQSRLAP NFNDRIMDVS 300
IKYINSCLGL DQSADEIAHC LKKMSLHAVQ SKEDKDILHV DIPVTRPDIL 350
HACDIMEDAA VGYGFNNLPK GEKLSNANFI AKPLPINKVS DIFRVASSQA 400
TWVEVLPLTL CSHDENFKFL RQSDNGDLAV KLANPKTLEY QVVRTTLLPG 450
ILKTVKENRK HSLPIKVFET GDVVFKDDKL ERKAYNERHW AAIYVGKNSG 500
FEIIQGLLGK IMQTFRTEWI ADYGAAASGR GYWIEEDDSV KTYFPGRGAK 550
VMFRSKEGAE PKQIGHLGVL HPEVMMNFDV PFAASFVEVN AEVFL 595
Length:595
Mass (Da):67,365
Last modified:January 23, 2007 - v3
Checksum:i68DA23E49644F801
GO

Sequence cautioni

The sequence AAA35151.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti18 – 181N → D AA sequence 1 Publication
Sequence conflicti426 – 4261G → D in AAA35151. 1 Publication
Sequence conflicti544 – 5441F → L in AAT92797. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03964 Genomic DNA. Translation: AAA35151.1. Different initiation.
X94607 Genomic DNA. Translation: CAA64307.1.
Z73232 Genomic DNA. Translation: CAA97591.1.
AY692778 Genomic DNA. Translation: AAT92797.1.
BK006945 Genomic DNA. Translation: DAA09378.1.
PIRiS61634. YFBYBC.
RefSeqiNP_013161.1. NM_001181947.1.

Genome annotation databases

EnsemblFungiiYLR060W; YLR060W; YLR060W.
GeneIDi850749.
KEGGisce:YLR060W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03964 Genomic DNA. Translation: AAA35151.1 . Different initiation.
X94607 Genomic DNA. Translation: CAA64307.1 .
Z73232 Genomic DNA. Translation: CAA97591.1 .
AY692778 Genomic DNA. Translation: AAT92797.1 .
BK006945 Genomic DNA. Translation: DAA09378.1 .
PIRi S61634. YFBYBC.
RefSeqi NP_013161.1. NM_001181947.1.

3D structure databases

ProteinModelPortali P15624.
SMRi P15624. Positions 1-595.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31335. 26 interactions.
DIPi DIP-6746N.
IntActi P15624. 5 interactions.
MINTi MINT-616734.
STRINGi 4932.YLR060W.

Proteomic databases

MaxQBi P15624.
PaxDbi P15624.
PeptideAtlasi P15624.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YLR060W ; YLR060W ; YLR060W .
GeneIDi 850749.
KEGGi sce:YLR060W.

Organism-specific databases

SGDi S000004050. FRS1.

Phylogenomic databases

eggNOGi COG0072.
GeneTreei ENSGT00530000063489.
HOGENOMi HOG000105095.
KOi K01890.
OMAi EVMPLIL.
OrthoDBi EOG77M8XG.

Enzyme and pathway databases

BioCyci YEAST:G3O-32214-MONOMER.

Miscellaneous databases

NextBioi 966879.
PROi P15624.

Gene expression databases

Genevestigatori P15624.

Family and domain databases

Gene3Di 3.30.56.20. 1 hit.
InterProi IPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR004531. Phe-tRNA-synth_IIc_bsu_arc.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view ]
Pfami PF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
[Graphical view ]
SMARTi SM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
[Graphical view ]
SUPFAMi SSF46955. SSF46955. 2 hits.
TIGRFAMsi TIGR00471. pheT_arch. 1 hit.
PROSITEi PS51483. B5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and expression of the genes encoding the alpha and beta subunits of yeast phenylalanyl-tRNA synthetase."
    Sanni A., Mirande M., Ebel J.-P., Boulanger Y., Waller J.-P., Fasiolo F.
    J. Biol. Chem. 263:15407-15415(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Phenylalanyl-tRNA synthetase from baker's yeast. Repeated sequences in the two subunits."
    Robbe-Saul S., Fasiolo F., Boulanger Y.
    FEBS Lett. 84:57-62(1977) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF N-TERMINUS, CLEAVAGE OF INITIATOR METHIONINE.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSYFB_YEAST
AccessioniPrimary (citable) accession number: P15624
Secondary accession number(s): D6VY62, E9P8X0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5440 molecules/cell in log phase SD medium.

Caution

Was originally (1 Publication) erroneously assigned as an alpha subunit.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3

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