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P15623 (GLNA_BACFR) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine synthetase

Short name=GS
EC=6.3.1.2
Alternative name(s):
Glutamate--ammonia ligase
Gene names
Name:glnA
Ordered Locus Names:BF1040
OrganismBacteroides fragilis (strain YCH46) [Complete proteome] [HAMAP]
Taxonomic identifier295405 [NCBI]
Taxonomic lineageBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides

Protein attributes

Sequence length729 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Subunit structure

Homohexamer.

Sequence similarities

Belongs to the glutamine synthetase family. Type 3 subfamily.

Ontologies

Keywords
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processnitrogen compound metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate-ammonia ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 729729Glutamine synthetase
PRO_0000153277

Experimental info

Sequence conflict1521V → G in AAA62314. Ref.1
Sequence conflict5311P → L in AAA62314. Ref.1
Sequence conflict5661T → M in AAA62314. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P15623 [UniParc].

Last modified November 23, 2004. Version 2.
Checksum: 1916071619EB9562

FASTA72982,825
        10         20         30         40         50         60 
MSKMRFFALQ ELSNRKPLEI TTPSNKLSDY YASHVFDRKK MQEYLPKEAY KAVVDATEKG 

        70         80         90        100        110        120 
TPISREMADL IANGMKSWAK SLNVTHYTHW FQPLTDGTAE KHDGFIEFGE DGEVIERFSG 

       130        140        150        160        170        180 
KLLIQQEPDA SSFPNGGIRN TFEARGYTAW DVSSPAFVVD TTLCIPTIFI SYTGEALDYK 

       190        200        210        220        230        240 
TPLLKALAAV DKAATEVCQL FDKNITRVFT NLGWEQEYFL VDTSLYNARP DLRLTGRTLM 

       250        260        270        280        290        300 
GHSSAKDQQL EDHYFGSIPP RVTAFMKELE IECHKLGIPV KTRHNEVAPN QFELAPIFEN 

       310        320        330        340        350        360 
CNLANDHNQL VMDLMKRIAR KHHFAVLFHE KPYNGVNGSG KHNNWSLCTD TGINLFAPGK 

       370        380        390        400        410        420 
NPKGNMLFLT FLVNVLMMVH KNQDLLRASI MSAGNSHRLG ANEAPPAILS IFLGSQLSAT 

       430        440        450        460        470        480 
LDEIVRQVTN SKMTPEEKTT LKLGIGRIPE ILLDTTDRNR TSPFAFTGNR FEFRAAGSSA 

       490        500        510        520        530        540 
NCAAAMIAIN AAMANQLNEF KASVDKLMEE GIGKDEAIFR ILKENIIASE PIRFEGDGYS 

       550        560        570        580        590        600 
EEWKQEAARR GLTNICHVPE ALMHYTDNQS RAVLIGERIF NETELACRLE VELEKYTMKV 

       610        620        630        640        650        660 
QIESRVLGDL AINHIVPIAV SYQNRLLENL CRMKEIFSEE EYEVMSADRK ELIKEISHRV 

       670        680        690        700        710        720 
SAIKVLVRDM TEARKVANHK ENFKEKAFAY EETVRPYLES IRDHIDHLEM EIDDEIWPLP 


KYRELLFTK 

« Hide

References

« Hide 'large scale' references
[1]"Molecular analysis of a novel glutamine synthetase of the anaerobe Bacteroides fragilis."
Hill R.T., Parker J.R., Goodman H.J.K., Jones D.T., Woods D.R.
J. Gen. Microbiol. 135:3271-3279(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: BF1.
[2]"Genomic analysis of Bacteroides fragilis reveals extensive DNA inversions regulating cell surface adaptation."
Kuwahara T., Yamashita A., Hirakawa H., Nakayama H., Toh H., Okada N., Kuhara S., Hattori M., Hayashi T., Ohnishi Y.
Proc. Natl. Acad. Sci. U.S.A. 101:14919-14924(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: YCH46.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M28252 Genomic DNA. Translation: AAA62314.1.
AP006841 Genomic DNA. Translation: BAD47790.1.
PIRA37191.
RefSeqYP_098324.1. NC_006347.1.

3D structure databases

ProteinModelPortalP15623.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-59116N.
STRING295405.BF1040.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD47790; BAD47790; BF1040.
GeneID3081476.
KEGGbfr:BF1040.
PATRIC21047818. VBIBacFra17906_1032.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3968.
HOGENOMHOG000024492.
KOK01915.
OMAGQQLDDH.
OrthoDBEOG6F2957.

Family and domain databases

Gene3D3.30.590.10. 2 hits.
InterProIPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR022147. GSIII_N.
[Graphical view]
PfamPF00120. Gln-synt_C. 1 hit.
PF12437. GSIII_N. 1 hit.
[Graphical view]
PROSITEPS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLNA_BACFR
AccessionPrimary (citable) accession number: P15623
Secondary accession number(s): Q64XI6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: November 23, 2004
Last modified: May 14, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families