Reviewed, UniProtKB/Swiss-Prot P15602 (NDUS7_PARTE)

Names and origin

Protein names

Recommended name:
    NADH-ubiquinone oxidoreductase 20 kDa subunit
    EC=1.6.5.3

Gene names

Name:	NAD10
Synonyms:	PSBG

Encoded on

Mitochondrion

Organism

Paramecium tetraurelia

Taxonomic identifier

5888 [NCBI]

Taxonomic lineage

Eukaryota › Alveolata › Ciliophora › Intramacronucleata › Oligohymenophorea › Peniculida › Parameciidae › Paramecium

Protein attributes

Sequence length	156 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	NADH + ubiquinone = NAD+ + ubiquinol.
Cofactor	Binds 1 4Fe-4S cluster Potential.
Subcellular location	Mitochondrion.
Sequence similarities	Belongs to the complex I 20 kDa subunit family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Mitochondrion
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 156	156	NADH-ubiquinone oxidoreductase 20 kDa subunit		PRO_0000118739
Sites
	Metal binding	33	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	34	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	98	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	128	1	Iron-sulfur (4Fe-4S) Potential
Experimental info
	Sequence conflict	123 – 127	5	MLCPR → IFVPG in AAA79257. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P15602-1 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: B056E34343D75C2F	FASTA	156	17,454
	10 20 30 40 50 60 MILKADFLKL SANNLISWAR QGSFWPLTFG LACCALEMMH ATVSRYDFDR FGVIFRATPR 70 80 90 100 110 120 QADLIIVAGT VTNKMAPALR RLYDQTADPK WVLSMGSCAN GGGYYHYSYA VVKGCDKIIP 130 140 150 VDMLCPRCPP TAEALFFGVL QLQKTLMKTI NEKKVF

References

[1]	"Nucleotide sequence of the mitochondrial genome of Paramecium." Pritchard A.E., Seilhamer J.J., Mahalingam R., Sable C.L., Venuti S.E., Cummings D.J. Nucleic Acids Res. 18:173-180(1990) [PubMed: 2308823] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Stock 51.
[2]	"An unusual region of Paramecium mitochondrial DNA containing chloroplast-like genes." Pritchard A.E., Venuti S.E., Ghalambor M.A., Sable C.L., Cummings D.J. Gene 78:121-134(1989) [PubMed: 2670676] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Stock 51.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X15917 Genomic DNA. Translation: CAA34045.1. M26930 Genomic DNA. Translation: AAA79257.1.
PIR	F2PPG. S07736.
3D structure databases
SMR	P15602. Positions 6-150.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.6.5.3. 21526.
Family and domain databases
InterPro	IPR006137. NADH_UbQ_OxRdtase-like_20kDa. IPR006138. NADH_UbQ_OxRdtase_20kDa_su. IPR014406. NiFe_hyd_3_ssu/Q_oxred_NuoB. [Graphical view]
PANTHER	PTHR11995:SF2. NADH_DH_20kDa. 1 hit. PTHR11995. NiFe_hyd_3_ssu/Q_oxred_NuoB. 1 hit.
Pfam	PF01058. Oxidored_q6. 1 hit. [Graphical view]
TIGRFAMs	TIGR01957. nuoB_fam. 1 hit.
PROSITE	PS01150. COMPLEX1_20K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDUS7_PARTE

Accession

Primary (citable) accession number: P15602
Secondary accession number(s): Q35365

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	February 9, 2010
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)