P15597 (VIRF_AGRT9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein virF

Gene names

Name:

virF

Encoded on

Plasmid pTi15955

Organism

Agrobacterium tumefaciens (strain 15955)

Taxonomic identifier

190386 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	202 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	In the host plant, component of SCF(virF) E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins such as the host VIP1, after its implication in T-DNA translocation to the host nucleus By similarity. Required for the formation of tumors of a wild-type size on certain plant species only.
Subunit structure	Component of SCF(virF) E3 ubiquitin ligase complexes. Interacts with host VIP1 and SKP1A By similarity.
Sequence similarities	Contains 1 F-box domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway Virulence
Disease	Crown gall tumor
Technical term	Plasmid
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ASK10	Q9C5T5	4	EBI-605118,EBI-607786	From a different organism.
SKP1A	Q39255	6	EBI-605118,EBI-532357	From a different organism.
SKP1B	Q9FHW7	5	EBI-605118,EBI-604076	From a different organism.
VIP1	Q9MA75	3	EBI-605118,EBI-606057	From a different organism.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 202	202	Protein virF		PRO_0000065870
Regions
	Domain	20 – 66	47	F-box

Sequences

	Sequence		Length	Mass (Da)
	P15597 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: 3B0E33E157C1ADD1	FASTA	202	22,437
	10 20 30 40 50 60 MRNSSLRDAS GSNDAQVPHK TELLNLPDHV LTEVAKRLAT NNPVESAENI ANFSKSHRFT 70 80 90 100 110 120 RDAVRTEPLE KFSSRLKILS RNAKLLSHAV RHAATLPDGE QLSEAQLSQM RSEVATRPVL 130 140 150 160 170 180 GVAYTHQDGQ PEERLSGNHL DHKINNIPNL VFNVAEPIMF NEISALEVMA EVRPIARSIK 190 200 TAHDDARAEL MSADRPRSTR GL

References

[1]

"Octopine and nopaline strains of Agrobacterium tumefaciens differ in virulence; molecular characterization of the virF locus." Melchers L.S., Maroney M.J., den Dulk-Ras A., Thompson D.V., van Vuuren H.A.J., Schilperoort R.A., Hooykaas P.J.J. Plant Mol. Biol. 14:249-259(1990) [PubMed: 2101693] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X13981 Genomic DNA. Translation: CAA32163.1.
PIR	S07979. S15915.
RefSeq	NP_059824.1. NC_002377.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P15597. 8 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1224343.
Phylogenomic databases
ProtClustDB	CLSK539983.
Family and domain databases
PROSITE	PS50181. FBOX. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VIRF_AGRT9

Accession

Primary (citable) accession number: P15597

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	September 21, 2011
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program