P15567 (PUR6_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylaminoimidazole carboxylase
EC=4.1.1.21

Alternative name(s):
AIR carboxylase
Short name=AIRC

Gene names

Name:	ade6
Synonyms:	min1
ORF Names:	SPCC1322.13

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	552 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.
Sequence similarities	In the C-terminal section; belongs to the AIR carboxylase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from sequence or structural similarity. Source: GeneDB_Spombe purine base metabolic process Inferred from sequence or structural similarity. Source: GeneDB_Spombe
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_Spombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ligase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: InterPro phosphoribosylaminoimidazole carboxylase activity Traceable author statement. Source: GeneDB_Spombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 552	552	Phosphoribosylaminoimidazole carboxylase		PRO_0000075027
Regions
	Domain	108 – 295	188	ATP-grasp
	Nucleotide binding	134 – 189	56	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P15567 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: 2C10AF7C4349808F	FASTA	552	60,013
	10 20 30 40 50 60 MSEKQVVGIL GGGQLGRMMV EAAHRLNIKC IILDAANSPA KQIDGGREHI DASFTDPDAI 70 80 90 100 110 120 VELSKKCTLL TTEIEHINTD ALAAVTKSVA VEPSPATLRC IQDKYLQKQH LQVFKIALPE 130 140 150 160 170 180 FCDAPDQESV EKAGQEFGYP FVLKSKTLAY DGRGNYVVHQ PSEIPTAIKA LGDRPLYVEK 190 200 210 220 230 240 FVPFSMEIAV MVVRSLDGKV YAYPTTETIQ KDNVCHLVYA PARLPFSIQQ RAQTLAMDAV 250 260 270 280 290 300 RTFEGAGIYG VEMFVLQDGE TILLNEIAPR PHNSGHYTIE ACPTSQFEAH LRAICGLPFS 310 320 330 340 350 360 EINTQLSTST THALMVNILG TDDPDYVSKI AKRSLSIPGA TLHLYGKAES RKGRKMGHVT 370 380 390 400 410 420 IISDSPQECE RRYQMLLDVK DPVESPVVGI IMGSDSDLSK MKDAAVILDE FKVPYELTIV 430 440 450 460 470 480 SAHRTPDRMV TYARTAASRG LRVIIAGAGG AAHLPGMVAA MTPLPVIGVP VKGSTLDGVD 490 500 510 520 530 540 SLHSIVQMPR GVPVATVAIN NSQNAGILAC RILATFQPSL LAAMESFMDN MKEIVLEKAD 550 KLEKVGWKNY SA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence analysis of the ade6 gene of Schizosaccharomyces pombe. Wild-type and mutant alleles including the recombination host spot allele ade6-M26." Szankasi P., Heyer W.-D., Schuchert P., Kohli J. J. Mol. Biol. 204:917-925(1988) [PubMed: 3221399] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M37264 Genomic DNA. Translation: AAA35285.1. X14488 Genomic DNA. Translation: CAA32650.1. CU329672 Genomic DNA. Translation: CAA22866.1.
PIR	DEZPP. S02159.
RefSeq	NP_588141.1. NM_001023131.1.
3D structure databases
ProteinModelPortal	P15567.
SMR	P15567. Positions 386-550.
ModBase	Search...
Protein-protein interaction databases
STRING	P15567.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPCC1322.13.1; SPCC1322.13.1:pep; SPCC1322.13.
GeneID	2538734.
GenomeReviews	Gene locus ade6 in contig CU329672_GR.
KEGG	spo:SPCC1322.13.
NMPDR	fig|4896.1.peg.479.
Organism-specific databases
GeneDB_Spombe	SPCC1322.13.
Phylogenomic databases
eggNOG	fuNOG05150.
GeneTree	EFGT00050000004356.
HOGENOM	HBG516369.
OMA	PLYAEKW.
OrthoDB	EOG4TXG17.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-000083-MONOMER.
BRENDA	4.1.1.21. 5615.
Gene expression databases
ArrayExpress	P15567.
Family and domain databases
InterPro	IPR016301. AIR_COase. IPR005875. AIR_COase_ATPase-su. IPR011761. ATP-grasp. IPR003135. ATP-grasp_carboxylate-amine. IPR013816. ATP_grasp_subdomain_2. IPR000031. N5-CAIR_Mutase_PurE_dom. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR011054. Rudment_hybrid_motif. [Graphical view]
Gene3D	G3DSA:3.40.50.7700. AIR_carboxyl. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
KO	K11808.
Pfam	PF00731. AIRC. 1 hit. PF02222. ATP-grasp. 1 hit. [Graphical view]
PIRSF	PIRSF001340. AIR_carboxylase. 1 hit.
SMART	SM01001. AIRC. 1 hit. [Graphical view]
SUPFAM	SSF52255. AIR_carboxyl. 1 hit. SSF52440. PreATP-grasp-like. 1 hit. SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMs	TIGR01162. PurE. 1 hit. TIGR01161. PurK. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR6_SCHPO

Accession

Primary (citable) accession number: P15567

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	January 25, 2012
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program