P15557 (PAC1_PSES3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acylase ACY 1

Cleaved into the following 2 chains:

1. Acylase ACY 1 large subunit
2. Acylase ACY 1 small subunit

Including the following 2 domains:

1. Cephalosporin acylase
   EC=3.5.1.-
   Alternative name(s):
   GL-7ACA acylase
2. Gamma-glutamyltranspeptidase
   Short name=GGT
   EC=2.3.2.2

Gene names

Name:

acyI

Organism

Pseudomonas sp. (strain SE83)

Taxonomic identifier

309 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria

Protein attributes

Sequence length	558 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Besides the cephalosporin acylase I activity which converts GL-7ACA into 7-ACA; this enzyme displays some gamma glutamyltranspeptidase activity.
Catalytic activity	7-beta-(4-carboxybutanamido)-cephalosporanic acid + H2O = 7-aminocephalosporanic acid + glutaric acid. (5-L-glutamyl)-peptide + an amino acid = peptide + 5-L-glutamyl amino acid. Glutathione + H2O = L-cysteinylglycine + L-glutamate.
Subunit structure	Dimer of two non-identical chains processed from the same precursor.
Sequence similarities	Belongs to the gamma-glutamyltransferase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Molecular function	Acyltransferase Hydrolase Transferase
PTM	Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	gamma-glutamyltransferase activity Inferred from electronic annotation. Source: EC hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – ?367	366	Acylase ACY 1 large subunit		PRO_0000011080
	Chain	368 – 558	191	Acylase ACY 1 small subunit		PRO_0000011081

Sequences

	Sequence		Length	Mass (Da)
	P15557 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 9659691DE0DC528B	FASTA	558	58,095
	10 20 30 40 50 60 MNAPVPVPRV ADFTCEKKPA SGSRGMVVTN HPLASAAGAQ ILLAGGNAID AAVASLFALT 70 80 90 100 110 120 VAEPMMVGIL GGGLSHIRLA DGRHVVIDNL STAPGKATAE MYECLSDEIG KQRDTRDRQN 130 140 150 160 170 180 VVGAKAVAVP GALKGWCEAL ARFGTLPLAE VLQPAIGLAE RGFVVTPYLS NCITDNAGDL 190 200 210 220 230 240 ARDPGLAAML LPGGKPLQPG MRLVQSDYAA SLKLIAAEGP DALYGGKLGR ALTDYMAANG 250 260 270 280 290 300 GLIDQADLAN YRIELREPIR GSYRGYEIIG PPPPSSSGVH ITQMLNILEG YDIGSLGFGS 310 320 330 340 350 360 TDAVHLLAEA LKIAFADRAV ATADPAFVKV PVARLIDKAY ADERRALIEM EQAKSWTAGL 370 380 390 400 410 420 SGGESADTTH VTVADAMGNV VSATQTINGL FGACVQIPGT GMIANNYMYN FDPHPGRALS 430 440 450 460 470 480 IAPGKRVFTS MAPMMALKEG RIAFALGLPG ALRIFPSALQ AIVNLIDHRM SLQEAVEAPR 490 500 510 520 530 540 VWTEGGVLEL EEAIPEAVAQ ALIARGHKVV RSPRVAGGMN AIAFNPDGTL TGAACWRADG 550 TPVAISGGLA RAGARFTI

References

[1]	"Nucleotide sequences of the genes for two distinct cephalosporin acylases from a Pseudomonas strain." Matsuda A., Toma K., Komatsu K. J. Bacteriol. 169:5821-5826(1987) [PubMed: 3680178] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-22 AND 368-388.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M18279 Genomic DNA. Translation: AAA88424.1.
PIR	B28392.
3D structure databases
ProteinModelPortal	P15557.
ModBase	Search...
Protein family/group databases
MEROPS	T03.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000101. GGT_peptidase. [Graphical view]
PANTHER	PTHR11686. GGT_peptidase. 1 hit.
Pfam	PF01019. G_glu_transpept. 1 hit. [Graphical view]
PRINTS	PR01210. GGTRANSPTASE.
TIGRFAMs	TIGR00066. G_glut_trans. 1 hit.
PROSITE	PS00462. G_GLU_TRANSPEPTIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PAC1_PSES3

Accession

Primary (citable) accession number: P15557

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	January 23, 2007
Last modified:	October 19, 2011
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program