Reviewed,
UniProtKB/Swiss-Prot P15540 (CP21A_PIG)
Last modified
October 13, 2009.
Version 74.
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Steroid 21-hydroxylase EC=1.14.99.10 Alternative name(s): Cytochrome P450 21 Cytochrome P450 XXI 21-OHase P450-C21 P-450c21 | ||||
| Gene names |
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| Organism | Sus scrofa (Pig) | ||||
| Taxonomic identifier | 9823 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus |
Protein attributes
| Sequence length | 492 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids. |
| Catalytic activity | A steroid + AH2 + O2 = a 21-hydroxysteroid + A + H2O. |
| Cofactor | Heme group By similarity. |
| Subcellular location | Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. |
| Domain | The leucine-rich hydrophobic amino acid N-terminal region probably helps to anchor the protein to the microsomal membrane. |
| Sequence similarities | Belongs to the cytochrome P450 family. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 492 | 492 | Steroid 21-hydroxylase | PRO_0000051978 | |||||
Regions | |||||||||
| Region | 341 – 357 | 17 | Steroid-binding Potential | ||||||
Sites | |||||||||
| Metal binding | 427 | 1 | Iron (heme axial ligand) By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 10 | 1 | L → T AA sequence Ref.1 | ||||||
| Sequence conflict | 13 | 1 | L → K AA sequence Ref.1 | ||||||
| Sequence conflict | 55 | 1 | R → K AA sequence Ref.1 | ||||||
| Sequence conflict | 141 | 1 | E → C AA sequence Ref.1 | ||||||
| Sequence conflict | 201 | 1 | E → D AA sequence Ref.1 | ||||||
| Sequence conflict | 391 | 1 | H → I AA sequence Ref.1 | ||||||
| Sequence conflict | 463 – 465 | 3 | HPH → VPY AA sequence Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Complete amino acid sequence of 21-hydroxylase cytochrome P-450 from porcine adrenal microsomes." Haniu M., Yanagibashi K., Hall P.F., Shively J.E. Arch. Biochem. Biophys. 254:380-384(1987) [PubMed: 3495238] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Adrenal gland. |
| [2] | "Sequences of the swine 21-hydroxylase gene (CYP21) and a portion of the opposite-strand overlapping gene of unknown function previously described in human." Burghelle-Mayeur C., Geffrotin C., Vaiman M. Biochim. Biophys. Acta 1171:153-161(1992) [PubMed: 1482677] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | "Detection of quantitative trait loci for fat androstenone levels in pigs." Quintanilla R., Demeure O., Bidanel J.P., Milan D., Iannuccelli N., Amigues Y., Gruand J., Renard C., Chevalet C., Bonneau M. J. Anim. Sci. 81:385-394(2003) [PubMed: 12643481] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Meishan. |
| [4] | Porcine genome sequencing project Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Duroc. |
| [5] | "The swine steroid 21-hydroxylase gene (CYP21): cloning and mapping within the swine leucocyte antigen complex." Geffrotin C., Chardon P., de Andres-Cara D.F., Feil R., Renard C., Vaiman M. Anim. Genet. 21:1-13(1990) [PubMed: 2109953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-99. |
Cross-references
Sequence databases | |
|---|---|
| M83939 Genomic DNA. Translation: AAA31080.1. AF490410 Genomic DNA. Translation: AAM11646.1. AL773560 Genomic DNA. Translation: CAN59658.1. | |
| PIR | A32525. S28169. |
| RefSeq | NP_999598.1. |
| UniGene | Ssc.52818 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1DT6 based on UniProtKB P00179. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 403337. |
| KEGG | ssc:403337. |
Organism-specific databases | |
| CTD | 403337. |
Phylogenomic databases | |
| HOVERGEN | P15540. |
Enzyme and pathway databases | |
| BRENDA | 1.14.99.10. 249. |
Family and domain databases | |
| InterPro | IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view] |
| Gene3D | G3DSA:1.10.630.10. Cyt_P450. 1 hit. |
| PANTHER | PTHR19383. Cyt_P450. 1 hit. |
| Pfam | PF00067. p450. 1 hit. [Graphical view] |
| PRINTS | PR00463. EP450I. PR00385. P450. |
| PROSITE | PS00086. CYTOCHROME_P450. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | CP21A_PIG | ||||||||
| Accession | Primary (citable) accession number: P15540 Secondary accession number(s): A5A8W6, Q02390 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||

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