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P15540

- CP21A_PIG

UniProt

P15540 - CP21A_PIG

Protein

Steroid 21-hydroxylase

Gene

CYP21

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 2 (26 Apr 2005)
      Previous versions | rss
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    Functioni

    Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids.

    Catalytic activityi

    A C(21) steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 21-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi427 – 4271Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. heme binding Source: InterPro
    2. iron ion binding Source: InterPro
    3. steroid 21-monooxygenase activity Source: UniProtKB-EC
    4. steroid binding Source: UniProtKB-KW

    GO - Biological processi

    1. steroid biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Steroidogenesis

    Keywords - Ligandi

    Heme, Iron, Lipid-binding, Metal-binding, Steroid-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Steroid 21-hydroxylase (EC:1.14.99.10)
    Alternative name(s):
    21-OHase
    Cytochrome P-450c21
    Cytochrome P450 21
    Cytochrome P450 XXI
    Cytochrome P450-C21
    Gene namesi
    Name:CYP21
    Synonyms:CYP21A1, CYP21A3
    OrganismiSus scrofa (Pig)
    Taxonomic identifieri9823 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
    ProteomesiUP000008227: Chromosome 7

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 492492Steroid 21-hydroxylasePRO_0000051978Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP15540.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni341 – 35717Steroid-bindingSequence AnalysisAdd
    BLAST

    Domaini

    The leucine-rich hydrophobic amino acid N-terminal region probably helps to anchor the protein to the microsomal membrane.

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiCOG2124.
    GeneTreeiENSGT00750000117317.
    HOGENOMiHOG000036991.
    HOVERGENiHBG106944.
    KOiK00513.
    OMAiVTIQKEF.
    TreeFamiTF105095.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P15540-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVLVWLLLLL TLLAGARLLW GQWKLRNLHL PPLVPGFLHL LQPNLPIYLL    50
    GLTQRLGPIY RLRLGLQDVV VLNSKRTIEE ALVRKWVDFA GRPQIPSYKL 100
    ASQHCPDISL GDYSLFWKAH KKLTRSALLL GVRSSMEPRV EQLTQEFCER 150
    MRAQAGTPVT IQKEFSVLTC SIICCLTFGD KEDTLVHALH DCVQDLMKTW 200
    EHWSIQILDM VPFLRFFPSP GLRRLKQAIE NRDHLVEKQL RRHKESMVAG 250
    QWRDMLDYML QEAGRQRVEE GQGQLLEGHV HMSVVDLFIG GTETTANTLS 300
    WAVVYLLHHP EIQWRLQEEL DRELGPGAAG SRVPYKDRAR LPLLNATIAE 350
    VLRLRPVVPL ALPHRATRPS SIFGYDIPEG TVVIPNLQGA HLDETVWEQP 400
    HEFRPDRFLA PGANPSALAF GCGARVCLGE PLARLELFVV LVQLLQAFTL 450
    LPPEGALPSL QPHPHSGINL KVQPFQVRLQ PRGGRGEGPG PR 492
    Length:492
    Mass (Da):55,620
    Last modified:April 26, 2005 - v2
    Checksum:i1FD5FFBFCA5A9E02
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti10 – 101L → T AA sequence (PubMed:3495238)Curated
    Sequence conflicti13 – 131L → K AA sequence (PubMed:3495238)Curated
    Sequence conflicti55 – 551R → K AA sequence (PubMed:3495238)Curated
    Sequence conflicti141 – 1411E → C AA sequence (PubMed:3495238)Curated
    Sequence conflicti201 – 2011E → D AA sequence (PubMed:3495238)Curated
    Sequence conflicti391 – 3911H → I AA sequence (PubMed:3495238)Curated
    Sequence conflicti463 – 4653HPH → VPY AA sequence (PubMed:3495238)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M83939 Genomic DNA. Translation: AAA31080.1.
    AF490410 Genomic DNA. Translation: AAM11646.1.
    AL773560 Genomic DNA. Translation: CAN59658.1.
    PIRiS28169. A32525.
    RefSeqiNP_999598.1. NM_214433.1.
    UniGeneiSsc.52818.

    Genome annotation databases

    EnsembliENSSSCT00000001571; ENSSSCP00000001529; ENSSSCG00000001428.
    GeneIDi403337.
    KEGGissc:403337.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M83939 Genomic DNA. Translation: AAA31080.1 .
    AF490410 Genomic DNA. Translation: AAM11646.1 .
    AL773560 Genomic DNA. Translation: CAN59658.1 .
    PIRi S28169. A32525.
    RefSeqi NP_999598.1. NM_214433.1.
    UniGenei Ssc.52818.

    3D structure databases

    ProteinModelPortali P15540.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSSSCT00000001571 ; ENSSSCP00000001529 ; ENSSSCG00000001428 .
    GeneIDi 403337.
    KEGGi ssc:403337.

    Organism-specific databases

    CTDi 1589.

    Phylogenomic databases

    eggNOGi COG2124.
    GeneTreei ENSGT00750000117317.
    HOGENOMi HOG000036991.
    HOVERGENi HBG106944.
    KOi K00513.
    OMAi VTIQKEF.
    TreeFami TF105095.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete amino acid sequence of 21-hydroxylase cytochrome P-450 from porcine adrenal microsomes."
      Haniu M., Yanagibashi K., Hall P.F., Shively J.E.
      Arch. Biochem. Biophys. 254:380-384(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE.
      Tissue: Adrenal gland.
    2. "Sequences of the swine 21-hydroxylase gene (CYP21) and a portion of the opposite-strand overlapping gene of unknown function previously described in human."
      Burghelle-Mayeur C., Geffrotin C., Vaiman M.
      Biochim. Biophys. Acta 1171:153-161(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Detection of quantitative trait loci for fat androstenone levels in pigs."
      Quintanilla R., Demeure O., Bidanel J.P., Milan D., Iannuccelli N., Amigues Y., Gruand J., Renard C., Chevalet C., Bonneau M.
      J. Anim. Sci. 81:385-394(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Meishan.
    4. Porcine genome sequencing project
      Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The swine steroid 21-hydroxylase gene (CYP21): cloning and mapping within the swine leucocyte antigen complex."
      Geffrotin C., Chardon P., de Andres-Cara D.F., Feil R., Renard C., Vaiman M.
      Anim. Genet. 21:1-13(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-99.

    Entry informationi

    Entry nameiCP21A_PIG
    AccessioniPrimary (citable) accession number: P15540
    Secondary accession number(s): A5A8W6, Q02390
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: April 26, 2005
    Last modified: October 1, 2014
    This is version 109 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3