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P15540 (CP21A_PIG) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Steroid 21-hydroxylase

EC=1.14.99.10
Alternative name(s):
21-OHase
Cytochrome P-450c21
Cytochrome P450 21
Cytochrome P450 XXI
Cytochrome P450-C21
Gene names
Name:CYP21
Synonyms:CYP21A1, CYP21A3
OrganismSus scrofa (Pig) [Reference proteome]
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus

Protein attributes

Sequence length492 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids.

Catalytic activity

A C21 steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 21-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Domain

The leucine-rich hydrophobic amino acid N-terminal region probably helps to anchor the protein to the microsomal membrane.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 492492Steroid 21-hydroxylase
PRO_0000051978

Regions

Region341 – 35717Steroid-binding Potential

Sites

Metal binding4271Iron (heme axial ligand) By similarity

Experimental info

Sequence conflict101L → T AA sequence Ref.1
Sequence conflict131L → K AA sequence Ref.1
Sequence conflict551R → K AA sequence Ref.1
Sequence conflict1411E → C AA sequence Ref.1
Sequence conflict2011E → D AA sequence Ref.1
Sequence conflict3911H → I AA sequence Ref.1
Sequence conflict463 – 4653HPH → VPY AA sequence Ref.1

Sequences

Sequence LengthMass (Da)Tools
P15540 [UniParc].

Last modified April 26, 2005. Version 2.
Checksum: 1FD5FFBFCA5A9E02

FASTA49255,620
        10         20         30         40         50         60 
MVLVWLLLLL TLLAGARLLW GQWKLRNLHL PPLVPGFLHL LQPNLPIYLL GLTQRLGPIY 

        70         80         90        100        110        120 
RLRLGLQDVV VLNSKRTIEE ALVRKWVDFA GRPQIPSYKL ASQHCPDISL GDYSLFWKAH 

       130        140        150        160        170        180 
KKLTRSALLL GVRSSMEPRV EQLTQEFCER MRAQAGTPVT IQKEFSVLTC SIICCLTFGD 

       190        200        210        220        230        240 
KEDTLVHALH DCVQDLMKTW EHWSIQILDM VPFLRFFPSP GLRRLKQAIE NRDHLVEKQL 

       250        260        270        280        290        300 
RRHKESMVAG QWRDMLDYML QEAGRQRVEE GQGQLLEGHV HMSVVDLFIG GTETTANTLS 

       310        320        330        340        350        360 
WAVVYLLHHP EIQWRLQEEL DRELGPGAAG SRVPYKDRAR LPLLNATIAE VLRLRPVVPL 

       370        380        390        400        410        420 
ALPHRATRPS SIFGYDIPEG TVVIPNLQGA HLDETVWEQP HEFRPDRFLA PGANPSALAF 

       430        440        450        460        470        480 
GCGARVCLGE PLARLELFVV LVQLLQAFTL LPPEGALPSL QPHPHSGINL KVQPFQVRLQ 

       490 
PRGGRGEGPG PR 

« Hide

References

« Hide 'large scale' references
[1]"Complete amino acid sequence of 21-hydroxylase cytochrome P-450 from porcine adrenal microsomes."
Haniu M., Yanagibashi K., Hall P.F., Shively J.E.
Arch. Biochem. Biophys. 254:380-384(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE.
Tissue: Adrenal gland.
[2]"Sequences of the swine 21-hydroxylase gene (CYP21) and a portion of the opposite-strand overlapping gene of unknown function previously described in human."
Burghelle-Mayeur C., Geffrotin C., Vaiman M.
Biochim. Biophys. Acta 1171:153-161(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Detection of quantitative trait loci for fat androstenone levels in pigs."
Quintanilla R., Demeure O., Bidanel J.P., Milan D., Iannuccelli N., Amigues Y., Gruand J., Renard C., Chevalet C., Bonneau M.
J. Anim. Sci. 81:385-394(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Meishan.
[4]Porcine genome sequencing project
Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The swine steroid 21-hydroxylase gene (CYP21): cloning and mapping within the swine leucocyte antigen complex."
Geffrotin C., Chardon P., de Andres-Cara D.F., Feil R., Renard C., Vaiman M.
Anim. Genet. 21:1-13(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-99.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M83939 Genomic DNA. Translation: AAA31080.1.
AF490410 Genomic DNA. Translation: AAM11646.1.
AL773560 Genomic DNA. Translation: CAN59658.1.
PIRA32525. S28169.
RefSeqNP_999598.1. NM_214433.1.
UniGeneSsc.52818.

3D structure databases

ProteinModelPortalP15540.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSSSCT00000001571; ENSSSCP00000001529; ENSSSCG00000001428.
GeneID403337.
KEGGssc:403337.

Organism-specific databases

CTD1589.

Phylogenomic databases

eggNOGCOG2124.
GeneTreeENSGT00750000117317.
HOGENOMHOG000036991.
HOVERGENHBG106944.
KOK00513.
OMAVTIQKEF.
TreeFamTF105095.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCP21A_PIG
AccessionPrimary (citable) accession number: P15540
Secondary accession number(s): A5A8W6, Q02390
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 26, 2005
Last modified: May 14, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families