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P15540

- CP21A_PIG

UniProt

P15540 - CP21A_PIG

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Protein

Steroid 21-hydroxylase

Gene

CYP21

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids.

Catalytic activityi

A C(21) steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 21-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O.

Cofactori

Heme group.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi427 – 4271Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. heme binding Source: InterPro
  2. iron ion binding Source: InterPro
  3. steroid 21-monooxygenase activity Source: UniProtKB-EC
  4. steroid binding Source: UniProtKB-KW

GO - Biological processi

  1. steroid biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Steroidogenesis

Keywords - Ligandi

Heme, Iron, Lipid-binding, Metal-binding, Steroid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Steroid 21-hydroxylase (EC:1.14.99.10)
Alternative name(s):
21-OHase
Cytochrome P-450c21
Cytochrome P450 21
Cytochrome P450 XXI
Cytochrome P450-C21
Gene namesi
Name:CYP21
Synonyms:CYP21A1, CYP21A3
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Chromosome 7

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 492492Steroid 21-hydroxylasePRO_0000051978Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP15540.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni341 – 35717Steroid-bindingSequence AnalysisAdd
BLAST

Domaini

The leucine-rich hydrophobic amino acid N-terminal region probably helps to anchor the protein to the microsomal membrane.

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118992.
HOGENOMiHOG000036991.
HOVERGENiHBG106944.
InParanoidiP15540.
KOiK00513.
OMAiVTIQKEF.
TreeFamiTF105095.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P15540-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVLVWLLLLL TLLAGARLLW GQWKLRNLHL PPLVPGFLHL LQPNLPIYLL
60 70 80 90 100
GLTQRLGPIY RLRLGLQDVV VLNSKRTIEE ALVRKWVDFA GRPQIPSYKL
110 120 130 140 150
ASQHCPDISL GDYSLFWKAH KKLTRSALLL GVRSSMEPRV EQLTQEFCER
160 170 180 190 200
MRAQAGTPVT IQKEFSVLTC SIICCLTFGD KEDTLVHALH DCVQDLMKTW
210 220 230 240 250
EHWSIQILDM VPFLRFFPSP GLRRLKQAIE NRDHLVEKQL RRHKESMVAG
260 270 280 290 300
QWRDMLDYML QEAGRQRVEE GQGQLLEGHV HMSVVDLFIG GTETTANTLS
310 320 330 340 350
WAVVYLLHHP EIQWRLQEEL DRELGPGAAG SRVPYKDRAR LPLLNATIAE
360 370 380 390 400
VLRLRPVVPL ALPHRATRPS SIFGYDIPEG TVVIPNLQGA HLDETVWEQP
410 420 430 440 450
HEFRPDRFLA PGANPSALAF GCGARVCLGE PLARLELFVV LVQLLQAFTL
460 470 480 490
LPPEGALPSL QPHPHSGINL KVQPFQVRLQ PRGGRGEGPG PR
Length:492
Mass (Da):55,620
Last modified:April 26, 2005 - v2
Checksum:i1FD5FFBFCA5A9E02
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti10 – 101L → T AA sequence (PubMed:3495238)Curated
Sequence conflicti13 – 131L → K AA sequence (PubMed:3495238)Curated
Sequence conflicti55 – 551R → K AA sequence (PubMed:3495238)Curated
Sequence conflicti141 – 1411E → C AA sequence (PubMed:3495238)Curated
Sequence conflicti201 – 2011E → D AA sequence (PubMed:3495238)Curated
Sequence conflicti391 – 3911H → I AA sequence (PubMed:3495238)Curated
Sequence conflicti463 – 4653HPH → VPY AA sequence (PubMed:3495238)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M83939 Genomic DNA. Translation: AAA31080.1.
AF490410 Genomic DNA. Translation: AAM11646.1.
AL773560 Genomic DNA. Translation: CAN59658.1.
PIRiS28169. A32525.
RefSeqiNP_999598.1. NM_214433.1.
UniGeneiSsc.52818.

Genome annotation databases

EnsembliENSSSCT00000001571; ENSSSCP00000001529; ENSSSCG00000001428.
GeneIDi403337.
KEGGissc:403337.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M83939 Genomic DNA. Translation: AAA31080.1 .
AF490410 Genomic DNA. Translation: AAM11646.1 .
AL773560 Genomic DNA. Translation: CAN59658.1 .
PIRi S28169. A32525.
RefSeqi NP_999598.1. NM_214433.1.
UniGenei Ssc.52818.

3D structure databases

ProteinModelPortali P15540.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSSSCT00000001571 ; ENSSSCP00000001529 ; ENSSSCG00000001428 .
GeneIDi 403337.
KEGGi ssc:403337.

Organism-specific databases

CTDi 1589.

Phylogenomic databases

eggNOGi COG2124.
GeneTreei ENSGT00760000118992.
HOGENOMi HOG000036991.
HOVERGENi HBG106944.
InParanoidi P15540.
KOi K00513.
OMAi VTIQKEF.
TreeFami TF105095.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete amino acid sequence of 21-hydroxylase cytochrome P-450 from porcine adrenal microsomes."
    Haniu M., Yanagibashi K., Hall P.F., Shively J.E.
    Arch. Biochem. Biophys. 254:380-384(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
    Tissue: Adrenal gland.
  2. "Sequences of the swine 21-hydroxylase gene (CYP21) and a portion of the opposite-strand overlapping gene of unknown function previously described in human."
    Burghelle-Mayeur C., Geffrotin C., Vaiman M.
    Biochim. Biophys. Acta 1171:153-161(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Detection of quantitative trait loci for fat androstenone levels in pigs."
    Quintanilla R., Demeure O., Bidanel J.P., Milan D., Iannuccelli N., Amigues Y., Gruand J., Renard C., Chevalet C., Bonneau M.
    J. Anim. Sci. 81:385-394(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Meishan.
  4. Porcine genome sequencing project
    Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The swine steroid 21-hydroxylase gene (CYP21): cloning and mapping within the swine leucocyte antigen complex."
    Geffrotin C., Chardon P., de Andres-Cara D.F., Feil R., Renard C., Vaiman M.
    Anim. Genet. 21:1-13(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-99.

Entry informationi

Entry nameiCP21A_PIG
AccessioniPrimary (citable) accession number: P15540
Secondary accession number(s): A5A8W6, Q02390
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 26, 2005
Last modified: October 29, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3