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Protein

Cytochrome P450 11B2, mitochondrial

Gene

Cyp11b2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Forms corticosterone from 11-deoxycorticosterone.

Catalytic activityi

A steroid + 2 reduced adrenodoxin + O2 + 2 H+ = an 11-beta- hydroxysteroid + 2 oxidized adrenodoxin + H2O.By similarity
Corticosterone + 2 reduced adrenodoxin + O2 + 2 H+ = 18-hydroxycorticosterone + 2 oxidized adrenodoxin + H2O.By similarity

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi447 – 4471Iron (heme axial ligand)By similarity

GO - Molecular functioni

GO - Biological processi

  • aldosterone biosynthetic process Source: MGI
  • C21-steroid hormone biosynthetic process Source: MGI
  • cortisol biosynthetic process Source: MGI
  • drinking behavior Source: MGI
  • ion homeostasis Source: MGI
  • regulation of blood pressure Source: MGI
  • regulation of blood volume by renal aldosterone Source: MGI
  • regulation of systemic arterial blood pressure by circulatory renin-angiotensin Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Steroidogenesis

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_281686. Endogenous sterols.
REACT_295803. Mineralocorticoid biosynthesis.
REACT_297756. Glucocorticoid biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 11B2, mitochondrial
Alternative name(s):
Aldosterone synthase
CYPXIB2
Cytochrome P450C11
Steroid 11-beta-hydroxylase (EC:1.14.15.4, EC:1.14.15.5)
Gene namesi
Name:Cyp11b2
Synonyms:Cyp11b-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:88584. Cyp11b2.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial membrane Source: UniProtKB-SubCell
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2424MitochondrionAdd
BLAST
Chaini25 – 500476Cytochrome P450 11B2, mitochondrialPRO_0000003600Add
BLAST

Proteomic databases

MaxQBiP15539.
PRIDEiP15539.

Expressioni

Gene expression databases

BgeeiP15539.
ExpressionAtlasiP15539. baseline and differential.
GenevisibleiP15539. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000131503.

Structurei

3D structure databases

ProteinModelPortaliP15539.
SMRiP15539. Positions 34-499.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000013161.
HOVERGENiHBG051098.
InParanoidiP15539.
KOiK00497.
PhylomeDBiP15539.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002399. Cyt_P450_mitochondrial.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00408. MITP450.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15539-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALRVTADVW LARPWQCLHR TRALGTTATL APKTLQPFEA IPQYSRNKWL
60 70 80 90 100
KMIQILREQG QENLHLEMHQ VFRELGPIFR HSVGKTQIVS VMLPEDAEKL
110 120 130 140 150
HQVESMLPRR MHLEPWVAHR ELRGLRRGVF LLNGPEWRLN RLRLNRNVLS
160 170 180 190 200
PKAVQKFVPM VDMVARDFLE TLKEKVLQNA RGSLTMDVQQ SLFNYTIEAS
210 220 230 240 250
NFALFGERLG LLGHDLSPGS LKFIHALHSM FKSTSQLLFL PKSLTRWTST
260 270 280 290 300
RVWKEHFDAW DVISEYANRC IWKVHQELRL GSSQTYSGIV AELISQGSLP
310 320 330 340 350
LDAIKANSME LTAGSVDTTA IPLVMTLFEL ARNPDVQKAL RQESLAAEAS
360 370 380 390 400
IAANPQKAMS DLPLLRAALK ETLRLYPVGG FLERILSSDL VLQNYHVPAG
410 420 430 440 450
TLVLLYLYSM GRNPAVFPRP ERYMPQRWLE RKRSFQHLAF GFGVRQCLGR
460 470 480 490 500
RLAEVEMMLL LHHILKTFQV ETLRQEDVQM AYRFVLMPSS SPVLTFRPVS
Length:500
Mass (Da):57,373
Last modified:July 27, 2011 - v3
Checksum:iDCAFFF3EF2663195
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti491 – 4911S → E in AAB21517 (PubMed:1686470).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S85260 Genomic DNA. Translation: AAB21517.2.
BC116908 mRNA. Translation: AAI16909.1.
BC119321 mRNA. Translation: AAI19322.1.
J04451 Genomic DNA. Translation: AAA50299.1.
PIRiA41552.
RefSeqiNP_034121.3. NM_009991.3.
UniGeneiMm.377079.

Genome annotation databases

GeneIDi13072.
KEGGimmu:13072.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S85260 Genomic DNA. Translation: AAB21517.2.
BC116908 mRNA. Translation: AAI16909.1.
BC119321 mRNA. Translation: AAI19322.1.
J04451 Genomic DNA. Translation: AAA50299.1.
PIRiA41552.
RefSeqiNP_034121.3. NM_009991.3.
UniGeneiMm.377079.

3D structure databases

ProteinModelPortaliP15539.
SMRiP15539. Positions 34-499.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000131503.

Proteomic databases

MaxQBiP15539.
PRIDEiP15539.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi13072.
KEGGimmu:13072.

Organism-specific databases

CTDi1585.
MGIiMGI:88584. Cyp11b2.

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000013161.
HOVERGENiHBG051098.
InParanoidiP15539.
KOiK00497.
PhylomeDBiP15539.

Enzyme and pathway databases

ReactomeiREACT_281686. Endogenous sterols.
REACT_295803. Mineralocorticoid biosynthesis.
REACT_297756. Glucocorticoid biosynthesis.

Miscellaneous databases

NextBioi283008.
PROiP15539.
SOURCEiSearch...

Gene expression databases

BgeeiP15539.
ExpressionAtlasiP15539. baseline and differential.
GenevisibleiP15539. MM.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002399. Cyt_P450_mitochondrial.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00408. MITP450.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Different isozymes of mouse 11 beta-hydroxylase produce mineralocorticoids and glucocorticoids."
    Domalik L.J., Chaplin D.D., Kirkman M.S., Wu R.C., Liu W., Howard T.A., Seldin M.F., Parker K.L.
    Mol. Endocrinol. 5:1853-1861(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. "Structural and functional analysis of the promoter region of the gene encoding mouse steroid 11 beta-hydroxylase."
    Mouw A.R., Rice D.A., Meade J.C., Chua S.C., White P.C., Schimmer B.P., Parker K.L.
    J. Biol. Chem. 264:1305-1309(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-42.

Entry informationi

Entry nameiC11B2_MOUSE
AccessioniPrimary (citable) accession number: P15539
Secondary accession number(s): Q14AB5, Q64661
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: July 27, 2011
Last modified: June 24, 2015
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.