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P15535

- B4GT1_MOUSE

UniProt

P15535 - B4GT1_MOUSE

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Protein

Beta-1,4-galactosyltransferase 1

Gene
B4galt1, Ggtb, Ggtb2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.
The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, as those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix.

Catalytic activityi

UDP-alpha-D-galactose + D-glucose = UDP + lactose.
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide.
UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.

Cofactori

Manganese By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi251 – 2511Manganese By similarity
Binding sitei311 – 3111UDP-alpha-D-galactose By similarity
Metal bindingi344 – 3441Manganese; via tele nitrogen By similarity
Binding sitei356 – 3561N-acetyl-D-glucosamine By similarity

GO - Molecular functioni

  1. beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity Source: MGI
  2. cytoskeletal protein binding Source: MGI
  3. galactosyltransferase activity Source: MGI
  4. lactose synthase activity Source: UniProtKB-EC
  5. manganese ion binding Source: UniProtKB
  6. N-acetyllactosamine synthase activity Source: UniProtKB-EC
  7. protein binding Source: MGI

GO - Biological processi

  1. acute inflammatory response Source: MGI
  2. angiogenesis involved in wound healing Source: MGI
  3. binding of sperm to zona pellucida Source: MGI
  4. branching morphogenesis of an epithelial tube Source: MGI
  5. cell adhesion Source: MGI
  6. development of secondary sexual characteristics Source: MGI
  7. epithelial cell development Source: MGI
  8. extracellular matrix organization Source: MGI
  9. galactose metabolic process Source: MGI
  10. glycoprotein biosynthetic process Source: MGI
  11. lactose biosynthetic process Source: MGI
  12. leukocyte migration Source: MGI
  13. mammary gland development Source: MGI
  14. negative regulation of cell proliferation Source: MGI
  15. oligosaccharide biosynthetic process Source: MGI
  16. penetration of zona pellucida Source: MGI
  17. positive regulation of apoptotic process Source: MGI
  18. positive regulation of apoptotic process involved in mammary gland involution Source: MGI
  19. positive regulation of epithelial cell proliferation involved in wound healing Source: MGI
  20. protein glycosylation Source: MGI
  21. protein N-linked glycosylation Source: UniProtKB
  22. regulation of acrosome reaction Source: MGI
  23. regulation of cell proliferation Source: MGI
  24. regulation of cellular component movement Source: MGI
  25. wound healing Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_196517. Pre-NOTCH Processing in Golgi.
REACT_198565. N-Glycan antennae elongation.
REACT_198578. Keratan sulfate biosynthesis.
REACT_199072. Interaction With The Zona Pellucida.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-galactosyltransferase 1 (EC:2.4.1.-)
Short name:
Beta-1,4-GalTase 1
Short name:
Beta4Gal-T1
Short name:
b4Gal-T1
Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1
Cleaved into the following chain:
Including the following 4 domains:
Lactose synthase A protein (EC:2.4.1.22)
N-acetyllactosamine synthase (EC:2.4.1.90)
Alternative name(s):
Nal synthase
Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase (EC:2.4.1.38)
Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase (EC:2.4.1.-)
Gene namesi
Name:B4galt1
Synonyms:Ggtb, Ggtb2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:95705. B4galt1.

Subcellular locationi

Chain Processed beta-1,4-galactosyltransferase 1 : Secreted
Note: Soluble form found in body fluids.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2424Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei25 – 4420Helical; Signal-anchor for type II membrane protein; Reviewed predictionAdd
BLAST
Topological domaini45 – 399355Lumenal Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. basolateral plasma membrane Source: Ensembl
  2. brush border membrane Source: Ensembl
  3. cell surface Source: MGI
  4. desmosome Source: Ensembl
  5. external side of plasma membrane Source: Ensembl
  6. extracellular region Source: UniProtKB-SubCell
  7. glycocalyx Source: Ensembl
  8. Golgi apparatus Source: MGI
  9. Golgi cisterna membrane Source: UniProtKB-SubCell
  10. Golgi trans cisterna Source: Ensembl
  11. integral component of membrane Source: UniProtKB-KW
  12. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Golgi apparatus, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 398Processed beta-1,4-galactosyltransferase 1PRO_0000296230
Chaini1 – 399399Beta-1,4-galactosyltransferase 1PRO_0000012280Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi113 – 1131N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi131 ↔ 173 By similarity
Disulfide bondi244 ↔ 263 By similarity

Post-translational modificationi

The soluble form derives from the membrane forms by proteolytic processing.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP15535.
PaxDbiP15535.
PRIDEiP15535.

PTM databases

PhosphoSiteiP15535.

Expressioni

Developmental stagei

In the brain, highest levels of expression are found at 11.5 dpc with decreased expression thereafter.1 Publication

Gene expression databases

ArrayExpressiP15535.
BgeeiP15535.
GenevestigatoriP15535.

Interactioni

Subunit structurei

Homodimer; and heterodimer with alpha-lactalbumin to form lactose synthase.

Protein-protein interaction databases

BioGridi199912. 2 interactions.
IntActiP15535. 1 interaction.
MINTiMINT-1864544.

Structurei

3D structure databases

ProteinModelPortaliP15535.
SMRiP15535. Positions 127-398.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni184 – 1885UDP-alpha-D-galactose binding By similarity
Regioni223 – 2253UDP-alpha-D-galactose binding By similarity
Regioni250 – 2512UDP-alpha-D-galactose binding By similarity
Regioni313 – 3164N-acetyl-D-glucosamine binding By similarity
Regioni344 – 3463UDP-alpha-D-galactose binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG327897.
HOGENOMiHOG000231027.
HOVERGENiHBG058334.
InParanoidiP15535.
KOiK07966.
OMAiVGGRYTP.
OrthoDBiEOG7060R0.
PhylomeDBiP15535.
TreeFamiTF312834.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. Align

Isoform Long (identifier: P15535-1) [UniParc]FASTAAdd to Basket

Also known as: Cell surface

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MRFREQFLGG SAAMPGATLQ RACRLLVAVC ALHLGVTLVY YLSGRDLSRL    50
PQLVGVSSTL QGGTNGAAAS KQPPGEQRPR GARPPPPLGV SPKPRPGLDS 100
SPGAASGPGL KSNLSSLPVP TTTGLLSLPA CPEESPLLVG PMLIDFNIAV 150
DLELLAKKNP EIKTGGRYSP KDCVSPHKVA IIIPFRNRQE HLKYWLYYLH 200
PILQRQQLDY GIYVINQAGD TMFNRAKLLN IGFQEALKDY DYNCFVFSDV 250
DLIPMDDRNA YRCFSQPRHI SVAMDKFGFS LPYVQYFGGV SALSKQQFLA 300
INGFPNNYWG WGGEDDDIFN RLVHKGMSIS RPNAVVGRCR MIRHSRDKKN 350
EPNPQRFDRI AHTKETMRFD GLNSLTYKVL DVQRYPLYTQ ITVDIGTPR 399
Length:399
Mass (Da):44,411
Last modified:April 1, 1990 - v1
Checksum:i084E3437115F4BDD
GO
Isoform Short (identifier: P15535-2) [UniParc]FASTAAdd to Basket

Also known as: Golgi complex

The sequence of this isoform differs from the canonical sequence as follows:
     1-13: Missing.

Show »
Length:386
Mass (Da):42,959
Checksum:i18D2F0BAF5387AAC
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1313Missing in isoform Short. VSP_018803Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03880 mRNA. Translation: AAA37297.1.
D00314 mRNA. Translation: BAA00216.1.
M27922
, M27917, M27918, M27919, M27920, M27921 Genomic DNA. Translation: AAA58745.1.
M27922
, M27917, M27918, M27919, M27920, M27921 Genomic DNA. Translation: AAA58744.1.
BC053006 mRNA. Translation: AAH53006.1.
M36289 mRNA. Translation: AAA37294.1.
L16840 mRNA. Translation: AAA62340.1.
CCDSiCCDS18051.1. [P15535-1]
PIRiA33396.
RefSeqiNP_071641.1. NM_022305.4. [P15535-1]
UniGeneiMm.15622.

Genome annotation databases

EnsembliENSMUST00000030121; ENSMUSP00000030121; ENSMUSG00000028413. [P15535-1]
GeneIDi14595.
KEGGimmu:14595.
UCSCiuc008shw.1. mouse. [P15535-1]

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

b4GalT1

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03880 mRNA. Translation: AAA37297.1 .
D00314 mRNA. Translation: BAA00216.1 .
M27922
, M27917 , M27918 , M27919 , M27920 , M27921 Genomic DNA. Translation: AAA58745.1 .
M27922
, M27917 , M27918 , M27919 , M27920 , M27921 Genomic DNA. Translation: AAA58744.1 .
BC053006 mRNA. Translation: AAH53006.1 .
M36289 mRNA. Translation: AAA37294.1 .
L16840 mRNA. Translation: AAA62340.1 .
CCDSi CCDS18051.1. [P15535-1 ]
PIRi A33396.
RefSeqi NP_071641.1. NM_022305.4. [P15535-1 ]
UniGenei Mm.15622.

3D structure databases

ProteinModelPortali P15535.
SMRi P15535. Positions 127-398.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 199912. 2 interactions.
IntActi P15535. 1 interaction.
MINTi MINT-1864544.

Protein family/group databases

CAZyi GT7. Glycosyltransferase Family 7.

PTM databases

PhosphoSitei P15535.

Proteomic databases

MaxQBi P15535.
PaxDbi P15535.
PRIDEi P15535.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000030121 ; ENSMUSP00000030121 ; ENSMUSG00000028413 . [P15535-1 ]
GeneIDi 14595.
KEGGi mmu:14595.
UCSCi uc008shw.1. mouse. [P15535-1 ]

Organism-specific databases

CTDi 2683.
MGIi MGI:95705. B4galt1.

Phylogenomic databases

eggNOGi NOG327897.
HOGENOMi HOG000231027.
HOVERGENi HBG058334.
InParanoidi P15535.
KOi K07966.
OMAi VGGRYTP.
OrthoDBi EOG7060R0.
PhylomeDBi P15535.
TreeFami TF312834.

Enzyme and pathway databases

UniPathwayi UPA00378 .
Reactomei REACT_196517. Pre-NOTCH Processing in Golgi.
REACT_198565. N-Glycan antennae elongation.
REACT_198578. Keratan sulfate biosynthesis.
REACT_199072. Interaction With The Zona Pellucida.

Miscellaneous databases

ChiTaRSi B4GALT1. mouse.
NextBioi 286356.
PROi P15535.
SOURCEi Search...

Gene expression databases

ArrayExpressi P15535.
Bgeei P15535.
Genevestigatori P15535.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
PANTHERi PTHR19300. PTHR19300. 1 hit.
Pfami PF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view ]
PRINTSi PR02050. B14GALTRFASE.
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the full length cDNA for murine beta-1,4-galactosyltransferase. Novel features at the 5'-end predict two translational start sites at two in-frame AUGs."
    Shaper N.L., Hollis G.F., Douglas J.G., Kirsch I.R., Shaper J.H.
    J. Biol. Chem. 263:10420-10428(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  2. "Murine beta 1,4-galactosyltransferase: both the amounts and structure of the mRNA are regulated during spermatogenesis."
    Shaper N.L., Wright W.W., Sharper J.H.
    Proc. Natl. Acad. Sci. U.S.A. 87:791-795(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Cloning and sequencing of a full-length cDNA of mouse N-acetylglucosamine (beta 1-4)galactosyltransferase."
    Nakazawa K., Ando T., Kimura T., Narimatsu H.
    J. Biochem. 104:165-168(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  6. "Evidence for two forms of murine beta-1,4-galactosyltransferase based on cloning studies."
    Shaper J.H., Hollis G.F., Shaper N.L.
    Biochimie 70:1683-1688(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 1-63.
  7. "Murine beta 1,4-galactosyltransferase: round spermatid transcripts are characterized by an extended 5'-untranslated region."
    Harduin-Lepers A., Shaper N.L., Mahoney J.A., Shaper J.H.
    Glycobiology 2:361-368(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 1-20.
    Strain: BALB/c.
  8. "Characterization of two cis-regulatory regions in the murine beta 1,4-galactosyltransferase gene. Evidence for a negative regulatory element that controls initiation at the proximal site."
    Harduin-Lepers A., Shaper J.H., Shaper N.L.
    J. Biol. Chem. 268:14348-14359(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-20.
    Strain: BALB/c.
  9. "Differential gene expression of beta-1,4-galactosyltransferases I, II and V during mouse brain development."
    Nakamura N., Yamakawa N., Sato T., Tojo H., Tachi C., Furukawa K.
    J. Neurochem. 76:29-38(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
    Strain: BALB/c.
    Tissue: Brain.

Entry informationi

Entry nameiB4GT1_MOUSE
AccessioniPrimary (citable) accession number: P15535
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: September 3, 2014
This is version 152 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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