P15535 (B4GT1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-1,4-galactosyltransferase 1
Short name=Beta-1,4-GalTase 1
Short name=Beta4Gal-T1
Short name=b4Gal-T1
EC=2.4.1.-

Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1

Cleaved into the following chain:

1. Processed beta-1,4-galactosyltransferase 1

Including the following 4 domains:

1. Lactose synthase A protein
   EC=2.4.1.22
2. N-acetyllactosamine synthase
   EC=2.4.1.90
   Alternative name(s):
   Nal synthase
3. Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase
   EC=2.4.1.38
4. Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase
   EC=2.4.1.-

Gene names

Name:	B4galt1
Synonyms:	Ggtb, Ggtb2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	399 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, as those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix.
Catalytic activity	UDP-alpha-D-galactose + D-glucose = UDP + lactose. UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide. UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.
Cofactor	Manganese By similarity.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Homodimer; and heterodimer with alpha-lactabulmin to form lactose synthase.
Subcellular location	Isoform Long: Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein. Cell surface. Note: Found in trans cisternae of Golgi. Isoform Short: Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein. Note: Found in trans cisternae of Golgi. Processed beta-1,4-galactosyltransferase 1: Secreted. Note: Soluble form found in body fluids.
Developmental stage	In the brain, highest levels of expression are found at 11.5 dpc with decreased expression thereafter. Ref.9
Post-translational modification	The soluble form derives from the membrane forms by proteolytic processing.
Sequence similarities	Belongs to the glycosyltransferase 7 family.

Ontologies

Keywords
Cellular component	Cell membrane Golgi apparatus Membrane Secreted
Coding sequence diversity	Alternative initiation
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Manganese Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	acute inflammatory response Inferred from mutant phenotype PubMed 12714507. Source: MGI angiogenesis involved in wound healing Inferred from mutant phenotype PubMed 15039218. Source: MGI binding of sperm to zona pellucida Inferred from mutant phenotype PubMed 9374408. Source: MGI branching morphogenesis of an epithelial tube Inferred from mutant phenotype PubMed 11900463PubMed 15282149. Source: MGI cell adhesion Inferred from genetic interaction PubMed 12714507. Source: MGI development of secondary sexual characteristics Inferred from mutant phenotype PubMed 11900463. Source: MGI epithelial cell development Inferred from mutant phenotype PubMed 9155011. Source: MGI extracellular matrix organization Inferred from mutant phenotype PubMed 11900463PubMed 8787759. Source: MGI galactose metabolic process Inferred from direct assay PubMed 9813328. Source: MGI lactose biosynthetic process Inferred from mutant phenotype PubMed 9155011. Source: MGI leukocyte migration Inferred from mutant phenotype PubMed 15039218. Source: MGI mammary gland development Inferred from mutant phenotype PubMed 11900463. Source: MGI negative regulation of cell proliferation Inferred from mutant phenotype PubMed 9155011. Source: MGI oligosaccharide biosynthetic process Inferred from electronic annotation. Source: Compara penetration of zona pellucida Inferred from mutant phenotype PubMed 9374408. Source: MGI positive regulation of apoptotic process involved in mammary gland involution Inferred from mutant phenotype PubMed 15282149. Source: MGI positive regulation of epithelial cell proliferation involved in wound healing Inferred from mutant phenotype PubMed 15039218. Source: MGI protein glycosylation Inferred from electronic annotation. Source: UniProtKB-UniPathway regulation of acrosome reaction Inferred from mutant phenotype PubMed 9374408. Source: MGI regulation of cellular component movement Inferred from direct assay PubMed 8134355. Source: MGI
Cellular_component	Golgi apparatus Inferred from direct assay PubMed 11375348PubMed 2503706. Source: MGI Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell Golgi trans cisterna Inferred from electronic annotation. Source: Compara basolateral plasma membrane Inferred from electronic annotation. Source: Compara brush border membrane Inferred from electronic annotation. Source: Compara cell surface Inferred from direct assay PubMed 7641815PubMed 8089187PubMed 8134355PubMed 9374408. Source: MGI desmosome Inferred from electronic annotation. Source: Compara external side of plasma membrane Inferred from electronic annotation. Source: Compara extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell glycocalyx Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay PubMed 11375348PubMed 2503706. Source: MGI
Molecular_function	N-acetyllactosamine synthase activity Inferred from electronic annotation. Source: EC beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity Inferred from direct assay PubMed 7641815PubMed 9813328. Source: MGI cytoskeletal protein binding Inferred from direct assay PubMed 7641815PubMed 8134355. Source: MGI lactose synthase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform Long (identifier: P15535-1)

Also known as: Cell surface;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: P15535-2)

Also known as: Golgi complex;

The sequence of this isoform differs from the canonical sequence as follows:
     1-13: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 399	399	Beta-1,4-galactosyltransferase 1		PRO_0000012280
	Chain	? – 398		Processed beta-1,4-galactosyltransferase 1		PRO_0000296230
Regions
	Topological domain	1 – 24	24	Cytoplasmic Potential
	Transmembrane	25 – 44	20	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	45 – 399	355	Lumenal Potential
Sites
	Metal binding	251	1	Manganese By similarity
	Metal binding	344	1	Manganese By similarity
Amino acid modifications
	Glycosylation	113	1	N-linked (GlcNAc...) Potential
	Disulfide bond	131 ↔ 173		By similarity
	Disulfide bond	244 ↔ 263		By similarity
Natural variations
	Alternative sequence	1 – 13	13	Missing in isoform Short.		VSP_018803

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long (Cell surface) [UniParc]. Last modified April 1, 1990. Version 1. Checksum: 084E3437115F4BDD	FASTA	399	44,411
	10 20 30 40 50 60 MRFREQFLGG SAAMPGATLQ RACRLLVAVC ALHLGVTLVY YLSGRDLSRL PQLVGVSSTL 70 80 90 100 110 120 QGGTNGAAAS KQPPGEQRPR GARPPPPLGV SPKPRPGLDS SPGAASGPGL KSNLSSLPVP 130 140 150 160 170 180 TTTGLLSLPA CPEESPLLVG PMLIDFNIAV DLELLAKKNP EIKTGGRYSP KDCVSPHKVA 190 200 210 220 230 240 IIIPFRNRQE HLKYWLYYLH PILQRQQLDY GIYVINQAGD TMFNRAKLLN IGFQEALKDY 250 260 270 280 290 300 DYNCFVFSDV DLIPMDDRNA YRCFSQPRHI SVAMDKFGFS LPYVQYFGGV SALSKQQFLA 310 320 330 340 350 360 INGFPNNYWG WGGEDDDIFN RLVHKGMSIS RPNAVVGRCR MIRHSRDKKN EPNPQRFDRI 370 380 390 AHTKETMRFD GLNSLTYKVL DVQRYPLYTQ ITVDIGTPR
	Isoform Short (Golgi complex) [UniParc]. Checksum: 18D2F0BAF5387AAC	FASTA	386	42,959
	10 20 30 40 50 60 MPGATLQRAC RLLVAVCALH LGVTLVYYLS GRDLSRLPQL VGVSSTLQGG TNGAAASKQP 70 80 90 100 110 120 PGEQRPRGAR PPPPLGVSPK PRPGLDSSPG AASGPGLKSN LSSLPVPTTT GLLSLPACPE 130 140 150 160 170 180 ESPLLVGPML IDFNIAVDLE LLAKKNPEIK TGGRYSPKDC VSPHKVAIII PFRNRQEHLK 190 200 210 220 230 240 YWLYYLHPIL QRQQLDYGIY VINQAGDTMF NRAKLLNIGF QEALKDYDYN CFVFSDVDLI 250 260 270 280 290 300 PMDDRNAYRC FSQPRHISVA MDKFGFSLPY VQYFGGVSAL SKQQFLAING FPNNYWGWGG 310 320 330 340 350 360 EDDDIFNRLV HKGMSISRPN AVVGRCRMIR HSRDKKNEPN PQRFDRIAHT KETMRFDGLN 370 380 SLTYKVLDVQ RYPLYTQITV DIGTPR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the full length cDNA for murine beta-1,4-galactosyltransferase. Novel features at the 5'-end predict two translational start sites at two in-frame AUGs." Shaper N.L., Hollis G.F., Douglas J.G., Kirsch I.R., Shaper J.H. J. Biol. Chem. 263:10420-10428(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c.
[2]	"Murine beta 1,4-galactosyltransferase: both the amounts and structure of the mRNA are regulated during spermatogenesis." Shaper N.L., Wright W.W., Sharper J.H. Proc. Natl. Acad. Sci. U.S.A. 87:791-795(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Genomic structure of murine beta-1,4-galactosyltransferase." Hollis G.F., Douglas J.G., Shaper N.L., Shaper J.H., Stafford-Hollis J.M., Evans R.J., Kirsch I.R. Biochem. Biophys. Res. Commun. 162:1069-1075(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Cloning and sequencing of a full-length cDNA of mouse N-acetylglucosamine (beta 1-4)galactosyltransferase." Nakazawa K., Ando T., Kimura T., Narimatsu H. J. Biochem. 104:165-168(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[6]	"Evidence for two forms of murine beta-1,4-galactosyltransferase based on cloning studies." Shaper J.H., Hollis G.F., Shaper N.L. Biochimie 70:1683-1688(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 1-63.
[7]	"Murine beta 1,4-galactosyltransferase: round spermatid transcripts are characterized by an extended 5'-untranslated region." Harduin-Lepers A., Shaper N.L., Mahoney J.A., Shaper J.H. Glycobiology 2:361-368(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 1-20. Strain: BALB/c.
[8]	"Characterization of two cis-regulatory regions in the murine beta 1,4-galactosyltransferase gene. Evidence for a negative regulatory element that controls initiation at the proximal site." Harduin-Lepers A., Shaper J.H., Shaper N.L. J. Biol. Chem. 268:14348-14359(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-20. Strain: BALB/c.
[9]	"Differential gene expression of beta-1,4-galactosyltransferases I, II and V during mouse brain development." Nakamura N., Yamakawa N., Sato T., Tojo H., Tachi C., Furukawa K. J. Neurochem. 76:29-38(2001) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE. Strain: BALB/c. Tissue: Brain.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - GTase

b4GalT1

Cross-references

Sequence databases
EMBL GenBank DDBJ	J03880 mRNA. Translation: AAA37297.1. D00314 mRNA. Translation: BAA00216.1. M27922 , M27917, M27918, M27919, M27920, M27921 Genomic DNA. Translation: AAA58745.1. M27922 , M27917, M27918, M27919, M27920, M27921 Genomic DNA. Translation: AAA58744.1. BC053006 mRNA. Translation: AAH53006.1. M36289 mRNA. Translation: AAA37294.1. L16840 mRNA. Translation: AAA62340.1.
IPI	IPI00131464. IPI00760067.
PIR	A33396.
RefSeq	NP_071641.1. NM_022305.4.
UniGene	Mm.15622.
3D structure databases
ProteinModelPortal	P15535.
SMR	P15535. Positions 127-398.
ModBase	Search...
Protein-protein interaction databases
IntAct	P15535. 1 interaction.
Protein family/group databases
CAZy	GT7. Glycosyltransferase Family 7.
PTM databases
PhosphoSite	P15535.
Proteomic databases
PaxDb	P15535.
PRIDE	P15535.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000030121; ENSMUSP00000030121; ENSMUSG00000028413.
GeneID	14595.
KEGG	mmu:14595.
Organism-specific databases
CTD	2683.
MGI	MGI:95705. B4galt1.
Phylogenomic databases
eggNOG	NOG327897.
HOGENOM	HOG000231027.
HOVERGEN	HBG058334.
InParanoid	P15535.
KO	K07966.
OMA	FNRLVFK.
OrthoDB	EOG4WWRK2.
Enzyme and pathway databases
UniPathway	UPA00378.
Gene expression databases
ArrayExpress	P15535.
Bgee	P15535.
Genevestigator	P15535.
GermOnline	ENSMUSG00000028413. Mus musculus.
Family and domain databases
InterPro	IPR003859. Galactosyl_T_2_met. [Graphical view]
PANTHER	PTHR19300. PTHR19300. 1 hit.
Pfam	PF02709. Glyco_transf_7C. 1 hit. [Graphical view]
PRINTS	PR02050. B14GALTRFASE.
ProtoNet	Search...
Other
ChiTaRS	B4GALT1. mouse.
NextBio	286356.
SOURCE	Search...

Entry information

Entry name

B4GT1_MOUSE

Accession

Primary (citable) accession number: P15535

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	May 1, 2013
This is version 139 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program