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Protein

Beta-1,4-galactosyltransferase 1

Gene

B4galt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.
The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, as those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix.

Catalytic activityi

UDP-alpha-D-galactose + D-glucose = UDP + lactose.
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide.
UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.

Cofactori

Mn2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi251ManganeseBy similarity1
Binding sitei311UDP-alpha-D-galactoseBy similarity1
Metal bindingi344Manganese; via tele nitrogenBy similarity1
Binding sitei356N-acetyl-D-glucosamineBy similarity1

GO - Molecular functioni

GO - Biological processi

  • acute inflammatory response Source: MGI
  • angiogenesis involved in wound healing Source: MGI
  • binding of sperm to zona pellucida Source: MGI
  • branching morphogenesis of an epithelial tube Source: MGI
  • cell adhesion Source: MGI
  • development of secondary sexual characteristics Source: MGI
  • epithelial cell development Source: MGI
  • extracellular matrix organization Source: MGI
  • galactose metabolic process Source: MGI
  • glycoprotein biosynthetic process Source: MGI
  • lactose biosynthetic process Source: MGI
  • leukocyte migration Source: MGI
  • mammary gland development Source: MGI
  • negative regulation of cell proliferation Source: MGI
  • oligosaccharide biosynthetic process Source: MGI
  • penetration of zona pellucida Source: MGI
  • positive regulation of apoptotic process Source: MGI
  • positive regulation of apoptotic process involved in mammary gland involution Source: MGI
  • positive regulation of epithelial cell proliferation involved in wound healing Source: MGI
  • protein glycosylation Source: MGI
  • protein homooligomerization Source: MGI
  • protein N-linked glycosylation Source: UniProtKB
  • regulation of acrosome reaction Source: MGI
  • regulation of cell proliferation Source: MGI
  • regulation of cellular component movement Source: MGI
  • wound healing Source: MGI

Keywordsi

Molecular functionGlycosyltransferase, Transferase
LigandManganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-1300644 Interaction With The Zona Pellucida
R-MMU-2022854 Keratan sulfate biosynthesis
R-MMU-5653890 Lactose synthesis
R-MMU-6798695 Neutrophil degranulation
R-MMU-975577 N-Glycan antennae elongation
UniPathwayiUPA00378

Protein family/group databases

CAZyiGT7 Glycosyltransferase Family 7

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-galactosyltransferase 1 (EC:2.4.1.-)
Short name:
Beta-1,4-GalTase 1
Short name:
Beta4Gal-T1
Short name:
b4Gal-T1
Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1
Cleaved into the following chain:
Including the following 4 domains:
Lactose synthase A protein (EC:2.4.1.22)
N-acetyllactosamine synthase (EC:2.4.1.90)
Alternative name(s):
Nal synthase
Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase (EC:2.4.1.38)
Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase (EC:2.4.1.-)
Gene namesi
Name:B4galt1
Synonyms:Ggtb, Ggtb2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:95705 B4galt1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 24CytoplasmicSequence analysisAdd BLAST24
Transmembranei25 – 44Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST20
Topological domaini45 – 399LumenalSequence analysisAdd BLAST355

Keywords - Cellular componenti

Cell membrane, Cell projection, Golgi apparatus, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000296230? – 398Processed beta-1,4-galactosyltransferase 1
ChainiPRO_00000122801 – 399Beta-1,4-galactosyltransferase 1Add BLAST399

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi113N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi131 ↔ 173By similarity
Disulfide bondi244 ↔ 263By similarity

Post-translational modificationi

The soluble form derives from the membrane forms by proteolytic processing.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP15535
PeptideAtlasiP15535
PRIDEiP15535

PTM databases

PhosphoSitePlusiP15535
SwissPalmiP15535

Expressioni

Developmental stagei

In the brain, highest levels of expression are found at 11.5 dpc with decreased expression thereafter.1 Publication

Gene expression databases

BgeeiENSMUSG00000028413
ExpressionAtlasiP15535 baseline and differential
GenevisibleiP15535 MM

Interactioni

Subunit structurei

Homodimer; and heterodimer with alpha-lactalbumin to form lactose synthase (By similarity). Interacts (via N-terminal cytoplasmic domain) with UBE2Q1 (via N-terminus); the interaction is direct (PubMed:18511602).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi19991212 interactors.
IntActiP15535 11 interactors.
STRINGi10090.ENSMUSP00000030121

Structurei

3D structure databases

ProteinModelPortaliP15535
SMRiP15535
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni184 – 188UDP-alpha-D-galactose bindingBy similarity5
Regioni223 – 225UDP-alpha-D-galactose bindingBy similarity3
Regioni250 – 251UDP-alpha-D-galactose bindingBy similarity2
Regioni313 – 316N-acetyl-D-glucosamine bindingBy similarity4
Regioni344 – 346UDP-alpha-D-galactose bindingBy similarity3

Sequence similaritiesi

Belongs to the glycosyltransferase 7 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3916 Eukaryota
ENOG410ZYYA LUCA
GeneTreeiENSGT00760000119140
HOGENOMiHOG000231027
HOVERGENiHBG058334
InParanoidiP15535
KOiK07966
OMAiAHTRETM
OrthoDBiEOG091G0P66
PhylomeDBiP15535
TreeFamiTF312834

Family and domain databases

Gene3Di3.90.550.101 hit
InterProiView protein in InterPro
IPR003859 Galactosyl_T
IPR027791 Galactosyl_T_C
IPR027995 Galactosyl_T_N
IPR029044 Nucleotide-diphossugar_trans
PANTHERiPTHR19300 PTHR19300, 1 hit
PfamiView protein in Pfam
PF02709 Glyco_transf_7C, 1 hit
PF13733 Glyco_transf_7N, 1 hit
PRINTSiPR02050 B14GALTRFASE
SUPFAMiSSF53448 SSF53448, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Long (identifier: P15535-1) [UniParc]FASTAAdd to basket
Also known as: Cell surface

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRFREQFLGG SAAMPGATLQ RACRLLVAVC ALHLGVTLVY YLSGRDLSRL
60 70 80 90 100
PQLVGVSSTL QGGTNGAAAS KQPPGEQRPR GARPPPPLGV SPKPRPGLDS
110 120 130 140 150
SPGAASGPGL KSNLSSLPVP TTTGLLSLPA CPEESPLLVG PMLIDFNIAV
160 170 180 190 200
DLELLAKKNP EIKTGGRYSP KDCVSPHKVA IIIPFRNRQE HLKYWLYYLH
210 220 230 240 250
PILQRQQLDY GIYVINQAGD TMFNRAKLLN IGFQEALKDY DYNCFVFSDV
260 270 280 290 300
DLIPMDDRNA YRCFSQPRHI SVAMDKFGFS LPYVQYFGGV SALSKQQFLA
310 320 330 340 350
INGFPNNYWG WGGEDDDIFN RLVHKGMSIS RPNAVVGRCR MIRHSRDKKN
360 370 380 390
EPNPQRFDRI AHTKETMRFD GLNSLTYKVL DVQRYPLYTQ ITVDIGTPR
Length:399
Mass (Da):44,411
Last modified:April 1, 1990 - v1
Checksum:i084E3437115F4BDD
GO
Isoform Short (identifier: P15535-2) [UniParc]FASTAAdd to basket
Also known as: Golgi complex

The sequence of this isoform differs from the canonical sequence as follows:
     1-13: Missing.

Show »
Length:386
Mass (Da):42,959
Checksum:i18D2F0BAF5387AAC
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0188031 – 13Missing in isoform Short. CuratedAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03880 mRNA Translation: AAA37297.1
D00314 mRNA Translation: BAA00216.1
M27922
, M27917, M27918, M27919, M27920, M27921 Genomic DNA Translation: AAA58745.1
M27922
, M27917, M27918, M27919, M27920, M27921 Genomic DNA Translation: AAA58744.1
BC053006 mRNA Translation: AAH53006.1
M36289 mRNA Translation: AAA37294.1
L16840 mRNA Translation: AAA62340.1
CCDSiCCDS18051.1 [P15535-1]
PIRiA33396
RefSeqiNP_071641.1, NM_022305.4 [P15535-1]
UniGeneiMm.15622

Genome annotation databases

EnsembliENSMUST00000030121; ENSMUSP00000030121; ENSMUSG00000028413 [P15535-1]
GeneIDi14595
KEGGimmu:14595
UCSCiuc008shw.2 mouse [P15535-1]

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Entry informationi

Entry nameiB4GT1_MOUSE
AccessioniPrimary (citable) accession number: P15535
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: February 28, 2018
This is version 182 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome