Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Beta-1,4-galactosyltransferase 1

Gene

B4galt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.
The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, as those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix.

Catalytic activityi

UDP-alpha-D-galactose + D-glucose = UDP + lactose.
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide.
UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.

Cofactori

Mn2+By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi251 – 2511ManganeseBy similarity
Binding sitei311 – 3111UDP-alpha-D-galactoseBy similarity
Metal bindingi344 – 3441Manganese; via tele nitrogenBy similarity
Binding sitei356 – 3561N-acetyl-D-glucosamineBy similarity

GO - Molecular functioni

  1. alpha-tubulin binding Source: MGI
  2. beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity Source: MGI
  3. beta-tubulin binding Source: MGI
  4. cytoskeletal protein binding Source: MGI
  5. galactosyltransferase activity Source: MGI
  6. lactose synthase activity Source: MGI
  7. manganese ion binding Source: UniProtKB
  8. N-acetyllactosamine synthase activity Source: MGI
  9. protein homodimerization activity Source: MGI
  10. UDP-galactosyltransferase activity Source: MGI

GO - Biological processi

  1. acute inflammatory response Source: MGI
  2. angiogenesis involved in wound healing Source: MGI
  3. binding of sperm to zona pellucida Source: MGI
  4. branching morphogenesis of an epithelial tube Source: MGI
  5. cell adhesion Source: MGI
  6. development of secondary sexual characteristics Source: MGI
  7. epithelial cell development Source: MGI
  8. extracellular matrix organization Source: MGI
  9. galactose metabolic process Source: MGI
  10. glycoprotein biosynthetic process Source: MGI
  11. lactose biosynthetic process Source: MGI
  12. leukocyte migration Source: MGI
  13. mammary gland development Source: MGI
  14. negative regulation of cell proliferation Source: MGI
  15. oligosaccharide biosynthetic process Source: MGI
  16. penetration of zona pellucida Source: MGI
  17. positive regulation of apoptotic process Source: MGI
  18. positive regulation of apoptotic process involved in mammary gland involution Source: MGI
  19. positive regulation of epithelial cell proliferation involved in wound healing Source: MGI
  20. protein glycosylation Source: MGI
  21. protein N-linked glycosylation Source: UniProtKB
  22. regulation of acrosome reaction Source: MGI
  23. regulation of cell proliferation Source: MGI
  24. regulation of cellular component movement Source: MGI
  25. wound healing Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_301180. Interaction With The Zona Pellucida.
REACT_308816. N-Glycan antennae elongation.
REACT_321348. Pre-NOTCH Processing in Golgi.
REACT_346228. Keratan sulfate biosynthesis.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-galactosyltransferase 1 (EC:2.4.1.-)
Short name:
Beta-1,4-GalTase 1
Short name:
Beta4Gal-T1
Short name:
b4Gal-T1
Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1
Cleaved into the following chain:
Including the following 4 domains:
Lactose synthase A protein (EC:2.4.1.22)
N-acetyllactosamine synthase (EC:2.4.1.90)
Alternative name(s):
Nal synthase
Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase (EC:2.4.1.38)
Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase (EC:2.4.1.-)
Gene namesi
Name:B4galt1
Synonyms:Ggtb, Ggtb2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:95705. B4galt1.

Subcellular locationi

Isoform Long : Golgi apparatusGolgi stack membrane 1 Publication; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein. Cell surface. Cell projectionfilopodium 1 Publication
Note: Found in trans cisternae of Golgi. B4GALT1 cell surface expression is regulated by UBE2Q1 (PubMed:18511602).1 Publication
Chain Processed beta-1,4-galactosyltransferase 1 : Secreted
Note: Soluble form found in body fluids.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2424CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei25 – 4420Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini45 – 399355LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. basolateral plasma membrane Source: MGI
  2. brush border membrane Source: MGI
  3. cell surface Source: MGI
  4. desmosome Source: MGI
  5. external side of plasma membrane Source: MGI
  6. extracellular space Source: MGI
  7. extracellular vesicular exosome Source: MGI
  8. filopodium Source: UniProtKB-SubCell
  9. glycocalyx Source: MGI
  10. Golgi apparatus Source: MGI
  11. Golgi cisterna membrane Source: UniProtKB-SubCell
  12. Golgi trans cisterna Source: MGI
  13. integral component of membrane Source: UniProtKB-KW
  14. membrane Source: MGI
  15. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Golgi apparatus, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 398Processed beta-1,4-galactosyltransferase 1PRO_0000296230
Chaini1 – 399399Beta-1,4-galactosyltransferase 1PRO_0000012280Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi113 – 1131N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi131 ↔ 173By similarity
Disulfide bondi244 ↔ 263By similarity

Post-translational modificationi

The soluble form derives from the membrane forms by proteolytic processing.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP15535.
PaxDbiP15535.
PRIDEiP15535.

PTM databases

PhosphoSiteiP15535.

Expressioni

Developmental stagei

In the brain, highest levels of expression are found at 11.5 dpc with decreased expression thereafter.1 Publication

Gene expression databases

BgeeiP15535.
ExpressionAtlasiP15535. baseline and differential.
GenevestigatoriP15535.

Interactioni

Subunit structurei

Homodimer; and heterodimer with alpha-lactalbumin to form lactose synthase (By similarity). Interacts (via N-terminal cytoplasmic domain) with UBE2Q1 (via N-terminus); the interaction is direct (PubMed:18511602).By similarity1 Publication

Protein-protein interaction databases

BioGridi199912. 2 interactions.
IntActiP15535. 1 interaction.
MINTiMINT-1864544.

Structurei

3D structure databases

ProteinModelPortaliP15535.
SMRiP15535. Positions 127-398.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni184 – 1885UDP-alpha-D-galactose bindingBy similarity
Regioni223 – 2253UDP-alpha-D-galactose bindingBy similarity
Regioni250 – 2512UDP-alpha-D-galactose bindingBy similarity
Regioni313 – 3164N-acetyl-D-glucosamine bindingBy similarity
Regioni344 – 3463UDP-alpha-D-galactose bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 7 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG327897.
HOGENOMiHOG000231027.
HOVERGENiHBG058334.
InParanoidiP15535.
KOiK07966.
OMAiAHTRETM.
OrthoDBiEOG7060R0.
PhylomeDBiP15535.
TreeFamiTF312834.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Long (identifier: P15535-1) [UniParc]FASTAAdd to basket

Also known as: Cell surface

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRFREQFLGG SAAMPGATLQ RACRLLVAVC ALHLGVTLVY YLSGRDLSRL
60 70 80 90 100
PQLVGVSSTL QGGTNGAAAS KQPPGEQRPR GARPPPPLGV SPKPRPGLDS
110 120 130 140 150
SPGAASGPGL KSNLSSLPVP TTTGLLSLPA CPEESPLLVG PMLIDFNIAV
160 170 180 190 200
DLELLAKKNP EIKTGGRYSP KDCVSPHKVA IIIPFRNRQE HLKYWLYYLH
210 220 230 240 250
PILQRQQLDY GIYVINQAGD TMFNRAKLLN IGFQEALKDY DYNCFVFSDV
260 270 280 290 300
DLIPMDDRNA YRCFSQPRHI SVAMDKFGFS LPYVQYFGGV SALSKQQFLA
310 320 330 340 350
INGFPNNYWG WGGEDDDIFN RLVHKGMSIS RPNAVVGRCR MIRHSRDKKN
360 370 380 390
EPNPQRFDRI AHTKETMRFD GLNSLTYKVL DVQRYPLYTQ ITVDIGTPR
Length:399
Mass (Da):44,411
Last modified:March 31, 1990 - v1
Checksum:i084E3437115F4BDD
GO
Isoform Short (identifier: P15535-2) [UniParc]FASTAAdd to basket

Also known as: Golgi complex

The sequence of this isoform differs from the canonical sequence as follows:
     1-13: Missing.

Show »
Length:386
Mass (Da):42,959
Checksum:i18D2F0BAF5387AAC
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1313Missing in isoform Short. CuratedVSP_018803Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03880 mRNA. Translation: AAA37297.1.
D00314 mRNA. Translation: BAA00216.1.
M27922
, M27917, M27918, M27919, M27920, M27921 Genomic DNA. Translation: AAA58745.1.
M27922
, M27917, M27918, M27919, M27920, M27921 Genomic DNA. Translation: AAA58744.1.
BC053006 mRNA. Translation: AAH53006.1.
M36289 mRNA. Translation: AAA37294.1.
L16840 mRNA. Translation: AAA62340.1.
CCDSiCCDS18051.1. [P15535-1]
PIRiA33396.
RefSeqiNP_071641.1. NM_022305.4. [P15535-1]
UniGeneiMm.15622.

Genome annotation databases

EnsembliENSMUST00000030121; ENSMUSP00000030121; ENSMUSG00000028413. [P15535-1]
GeneIDi14595.
KEGGimmu:14595.
UCSCiuc008shw.1. mouse. [P15535-1]

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

b4GalT1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03880 mRNA. Translation: AAA37297.1.
D00314 mRNA. Translation: BAA00216.1.
M27922
, M27917, M27918, M27919, M27920, M27921 Genomic DNA. Translation: AAA58745.1.
M27922
, M27917, M27918, M27919, M27920, M27921 Genomic DNA. Translation: AAA58744.1.
BC053006 mRNA. Translation: AAH53006.1.
M36289 mRNA. Translation: AAA37294.1.
L16840 mRNA. Translation: AAA62340.1.
CCDSiCCDS18051.1. [P15535-1]
PIRiA33396.
RefSeqiNP_071641.1. NM_022305.4. [P15535-1]
UniGeneiMm.15622.

3D structure databases

ProteinModelPortaliP15535.
SMRiP15535. Positions 127-398.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199912. 2 interactions.
IntActiP15535. 1 interaction.
MINTiMINT-1864544.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

PTM databases

PhosphoSiteiP15535.

Proteomic databases

MaxQBiP15535.
PaxDbiP15535.
PRIDEiP15535.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030121; ENSMUSP00000030121; ENSMUSG00000028413. [P15535-1]
GeneIDi14595.
KEGGimmu:14595.
UCSCiuc008shw.1. mouse. [P15535-1]

Organism-specific databases

CTDi2683.
MGIiMGI:95705. B4galt1.

Phylogenomic databases

eggNOGiNOG327897.
HOGENOMiHOG000231027.
HOVERGENiHBG058334.
InParanoidiP15535.
KOiK07966.
OMAiAHTRETM.
OrthoDBiEOG7060R0.
PhylomeDBiP15535.
TreeFamiTF312834.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiREACT_301180. Interaction With The Zona Pellucida.
REACT_308816. N-Glycan antennae elongation.
REACT_321348. Pre-NOTCH Processing in Golgi.
REACT_346228. Keratan sulfate biosynthesis.

Miscellaneous databases

ChiTaRSiB4galt1. mouse.
NextBioi286356.
PROiP15535.
SOURCEiSearch...

Gene expression databases

BgeeiP15535.
ExpressionAtlasiP15535. baseline and differential.
GenevestigatoriP15535.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the full length cDNA for murine beta-1,4-galactosyltransferase. Novel features at the 5'-end predict two translational start sites at two in-frame AUGs."
    Shaper N.L., Hollis G.F., Douglas J.G., Kirsch I.R., Shaper J.H.
    J. Biol. Chem. 263:10420-10428(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  2. "Murine beta 1,4-galactosyltransferase: both the amounts and structure of the mRNA are regulated during spermatogenesis."
    Shaper N.L., Wright W.W., Sharper J.H.
    Proc. Natl. Acad. Sci. U.S.A. 87:791-795(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Cloning and sequencing of a full-length cDNA of mouse N-acetylglucosamine (beta 1-4)galactosyltransferase."
    Nakazawa K., Ando T., Kimura T., Narimatsu H.
    J. Biochem. 104:165-168(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  6. "Evidence for two forms of murine beta-1,4-galactosyltransferase based on cloning studies."
    Shaper J.H., Hollis G.F., Shaper N.L.
    Biochimie 70:1683-1688(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 1-63.
  7. "Murine beta 1,4-galactosyltransferase: round spermatid transcripts are characterized by an extended 5'-untranslated region."
    Harduin-Lepers A., Shaper N.L., Mahoney J.A., Shaper J.H.
    Glycobiology 2:361-368(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 1-20.
    Strain: BALB/c.
  8. "Characterization of two cis-regulatory regions in the murine beta 1,4-galactosyltransferase gene. Evidence for a negative regulatory element that controls initiation at the proximal site."
    Harduin-Lepers A., Shaper J.H., Shaper N.L.
    J. Biol. Chem. 268:14348-14359(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-20.
    Strain: BALB/c.
  9. "Differential gene expression of beta-1,4-galactosyltransferases I, II and V during mouse brain development."
    Nakamura N., Yamakawa N., Sato T., Tojo H., Tachi C., Furukawa K.
    J. Neurochem. 76:29-38(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
    Strain: BALB/c.
    Tissue: Brain.
  10. "Characterization of a novel ubiquitin-conjugating enzyme that regulates beta1,4-galactosyltransferase-1 in embryonic stem cells."
    Wassler M.J., Shur B.D., Zhou W., Geng Y.J.
    Stem Cells 26:2006-2018(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH UBE2Q1.

Entry informationi

Entry nameiB4GT1_MOUSE
AccessioniPrimary (citable) accession number: P15535
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 31, 1990
Last sequence update: March 31, 1990
Last modified: March 31, 2015
This is version 159 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.