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P15535

- B4GT1_MOUSE

UniProt

P15535 - B4GT1_MOUSE

Protein

Beta-1,4-galactosyltransferase 1

Gene

B4galt1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 153 (01 Oct 2014)
      Sequence version 1 (01 Apr 1990)
      Previous versions | rss
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    Functioni

    The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.
    The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, as those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix.

    Catalytic activityi

    UDP-alpha-D-galactose + D-glucose = UDP + lactose.
    UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide.
    UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.

    Cofactori

    Manganese.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi251 – 2511ManganeseBy similarity
    Binding sitei311 – 3111UDP-alpha-D-galactoseBy similarity
    Metal bindingi344 – 3441Manganese; via tele nitrogenBy similarity
    Binding sitei356 – 3561N-acetyl-D-glucosamineBy similarity

    GO - Molecular functioni

    1. beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity Source: MGI
    2. cytoskeletal protein binding Source: MGI
    3. galactosyltransferase activity Source: MGI
    4. lactose synthase activity Source: UniProtKB-EC
    5. manganese ion binding Source: UniProtKB
    6. N-acetyllactosamine synthase activity Source: UniProtKB-EC
    7. protein binding Source: MGI

    GO - Biological processi

    1. acute inflammatory response Source: MGI
    2. angiogenesis involved in wound healing Source: MGI
    3. binding of sperm to zona pellucida Source: MGI
    4. branching morphogenesis of an epithelial tube Source: MGI
    5. cell adhesion Source: MGI
    6. development of secondary sexual characteristics Source: MGI
    7. epithelial cell development Source: MGI
    8. extracellular matrix organization Source: MGI
    9. galactose metabolic process Source: MGI
    10. glycoprotein biosynthetic process Source: MGI
    11. lactose biosynthetic process Source: MGI
    12. leukocyte migration Source: MGI
    13. mammary gland development Source: MGI
    14. negative regulation of cell proliferation Source: MGI
    15. oligosaccharide biosynthetic process Source: MGI
    16. penetration of zona pellucida Source: MGI
    17. positive regulation of apoptotic process Source: MGI
    18. positive regulation of apoptotic process involved in mammary gland involution Source: MGI
    19. positive regulation of epithelial cell proliferation involved in wound healing Source: MGI
    20. protein glycosylation Source: MGI
    21. protein N-linked glycosylation Source: UniProtKB
    22. regulation of acrosome reaction Source: MGI
    23. regulation of cell proliferation Source: MGI
    24. regulation of cellular component movement Source: MGI
    25. wound healing Source: MGI

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_196517. Pre-NOTCH Processing in Golgi.
    REACT_198565. N-Glycan antennae elongation.
    REACT_198578. Keratan sulfate biosynthesis.
    REACT_199072. Interaction With The Zona Pellucida.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT7. Glycosyltransferase Family 7.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-1,4-galactosyltransferase 1 (EC:2.4.1.-)
    Short name:
    Beta-1,4-GalTase 1
    Short name:
    Beta4Gal-T1
    Short name:
    b4Gal-T1
    Alternative name(s):
    UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1
    UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1
    Cleaved into the following chain:
    Including the following 4 domains:
    Lactose synthase A protein (EC:2.4.1.22)
    N-acetyllactosamine synthase (EC:2.4.1.90)
    Alternative name(s):
    Nal synthase
    Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase (EC:2.4.1.38)
    Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase (EC:2.4.1.-)
    Gene namesi
    Name:B4galt1
    Synonyms:Ggtb, Ggtb2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:95705. B4galt1.

    Subcellular locationi

    Chain Processed beta-1,4-galactosyltransferase 1 : Secreted
    Note: Soluble form found in body fluids.

    GO - Cellular componenti

    1. basolateral plasma membrane Source: Ensembl
    2. brush border membrane Source: Ensembl
    3. cell surface Source: MGI
    4. desmosome Source: Ensembl
    5. external side of plasma membrane Source: Ensembl
    6. extracellular region Source: UniProtKB-SubCell
    7. glycocalyx Source: Ensembl
    8. Golgi apparatus Source: MGI
    9. Golgi cisterna membrane Source: UniProtKB-SubCell
    10. Golgi trans cisterna Source: Ensembl
    11. integral component of membrane Source: UniProtKB-KW
    12. plasma membrane Source: MGI

    Keywords - Cellular componenti

    Cell membrane, Golgi apparatus, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 398Processed beta-1,4-galactosyltransferase 1PRO_0000296230
    Chaini1 – 399399Beta-1,4-galactosyltransferase 1PRO_0000012280Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi113 – 1131N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi131 ↔ 173By similarity
    Disulfide bondi244 ↔ 263By similarity

    Post-translational modificationi

    The soluble form derives from the membrane forms by proteolytic processing.

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiP15535.
    PaxDbiP15535.
    PRIDEiP15535.

    PTM databases

    PhosphoSiteiP15535.

    Expressioni

    Developmental stagei

    In the brain, highest levels of expression are found at 11.5 dpc with decreased expression thereafter.1 Publication

    Gene expression databases

    ArrayExpressiP15535.
    BgeeiP15535.
    GenevestigatoriP15535.

    Interactioni

    Subunit structurei

    Homodimer; and heterodimer with alpha-lactalbumin to form lactose synthase.

    Protein-protein interaction databases

    BioGridi199912. 2 interactions.
    IntActiP15535. 1 interaction.
    MINTiMINT-1864544.

    Structurei

    3D structure databases

    ProteinModelPortaliP15535.
    SMRiP15535. Positions 127-398.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2424CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini45 – 399355LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei25 – 4420Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni184 – 1885UDP-alpha-D-galactose bindingBy similarity
    Regioni223 – 2253UDP-alpha-D-galactose bindingBy similarity
    Regioni250 – 2512UDP-alpha-D-galactose bindingBy similarity
    Regioni313 – 3164N-acetyl-D-glucosamine bindingBy similarity
    Regioni344 – 3463UDP-alpha-D-galactose bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyltransferase 7 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG327897.
    HOGENOMiHOG000231027.
    HOVERGENiHBG058334.
    InParanoidiP15535.
    KOiK07966.
    OMAiVGGRYTP.
    OrthoDBiEOG7060R0.
    PhylomeDBiP15535.
    TreeFamiTF312834.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR003859. Galactosyl_T.
    IPR027791. Galactosyl_T_C.
    IPR027995. Galactosyl_T_N.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR19300. PTHR19300. 1 hit.
    PfamiPF02709. Glyco_transf_7C. 1 hit.
    PF13733. Glyco_transf_7N. 1 hit.
    [Graphical view]
    PRINTSiPR02050. B14GALTRFASE.
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative initiation. Align

    Isoform Long (identifier: P15535-1) [UniParc]FASTAAdd to Basket

    Also known as: Cell surface

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRFREQFLGG SAAMPGATLQ RACRLLVAVC ALHLGVTLVY YLSGRDLSRL    50
    PQLVGVSSTL QGGTNGAAAS KQPPGEQRPR GARPPPPLGV SPKPRPGLDS 100
    SPGAASGPGL KSNLSSLPVP TTTGLLSLPA CPEESPLLVG PMLIDFNIAV 150
    DLELLAKKNP EIKTGGRYSP KDCVSPHKVA IIIPFRNRQE HLKYWLYYLH 200
    PILQRQQLDY GIYVINQAGD TMFNRAKLLN IGFQEALKDY DYNCFVFSDV 250
    DLIPMDDRNA YRCFSQPRHI SVAMDKFGFS LPYVQYFGGV SALSKQQFLA 300
    INGFPNNYWG WGGEDDDIFN RLVHKGMSIS RPNAVVGRCR MIRHSRDKKN 350
    EPNPQRFDRI AHTKETMRFD GLNSLTYKVL DVQRYPLYTQ ITVDIGTPR 399
    Length:399
    Mass (Da):44,411
    Last modified:April 1, 1990 - v1
    Checksum:i084E3437115F4BDD
    GO
    Isoform Short (identifier: P15535-2) [UniParc]FASTAAdd to Basket

    Also known as: Golgi complex

    The sequence of this isoform differs from the canonical sequence as follows:
         1-13: Missing.

    Show »
    Length:386
    Mass (Da):42,959
    Checksum:i18D2F0BAF5387AAC
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 1313Missing in isoform Short. CuratedVSP_018803Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03880 mRNA. Translation: AAA37297.1.
    D00314 mRNA. Translation: BAA00216.1.
    M27922
    , M27917, M27918, M27919, M27920, M27921 Genomic DNA. Translation: AAA58745.1.
    M27922
    , M27917, M27918, M27919, M27920, M27921 Genomic DNA. Translation: AAA58744.1.
    BC053006 mRNA. Translation: AAH53006.1.
    M36289 mRNA. Translation: AAA37294.1.
    L16840 mRNA. Translation: AAA62340.1.
    CCDSiCCDS18051.1. [P15535-1]
    PIRiA33396.
    RefSeqiNP_071641.1. NM_022305.4. [P15535-1]
    UniGeneiMm.15622.

    Genome annotation databases

    EnsembliENSMUST00000030121; ENSMUSP00000030121; ENSMUSG00000028413. [P15535-1]
    GeneIDi14595.
    KEGGimmu:14595.
    UCSCiuc008shw.1. mouse. [P15535-1]

    Keywords - Coding sequence diversityi

    Alternative initiation

    Cross-referencesi

    Web resourcesi

    Functional Glycomics Gateway - GTase

    b4GalT1

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03880 mRNA. Translation: AAA37297.1 .
    D00314 mRNA. Translation: BAA00216.1 .
    M27922
    , M27917 , M27918 , M27919 , M27920 , M27921 Genomic DNA. Translation: AAA58745.1 .
    M27922
    , M27917 , M27918 , M27919 , M27920 , M27921 Genomic DNA. Translation: AAA58744.1 .
    BC053006 mRNA. Translation: AAH53006.1 .
    M36289 mRNA. Translation: AAA37294.1 .
    L16840 mRNA. Translation: AAA62340.1 .
    CCDSi CCDS18051.1. [P15535-1 ]
    PIRi A33396.
    RefSeqi NP_071641.1. NM_022305.4. [P15535-1 ]
    UniGenei Mm.15622.

    3D structure databases

    ProteinModelPortali P15535.
    SMRi P15535. Positions 127-398.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199912. 2 interactions.
    IntActi P15535. 1 interaction.
    MINTi MINT-1864544.

    Protein family/group databases

    CAZyi GT7. Glycosyltransferase Family 7.

    PTM databases

    PhosphoSitei P15535.

    Proteomic databases

    MaxQBi P15535.
    PaxDbi P15535.
    PRIDEi P15535.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000030121 ; ENSMUSP00000030121 ; ENSMUSG00000028413 . [P15535-1 ]
    GeneIDi 14595.
    KEGGi mmu:14595.
    UCSCi uc008shw.1. mouse. [P15535-1 ]

    Organism-specific databases

    CTDi 2683.
    MGIi MGI:95705. B4galt1.

    Phylogenomic databases

    eggNOGi NOG327897.
    HOGENOMi HOG000231027.
    HOVERGENi HBG058334.
    InParanoidi P15535.
    KOi K07966.
    OMAi VGGRYTP.
    OrthoDBi EOG7060R0.
    PhylomeDBi P15535.
    TreeFami TF312834.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    Reactomei REACT_196517. Pre-NOTCH Processing in Golgi.
    REACT_198565. N-Glycan antennae elongation.
    REACT_198578. Keratan sulfate biosynthesis.
    REACT_199072. Interaction With The Zona Pellucida.

    Miscellaneous databases

    ChiTaRSi B4GALT1. mouse.
    NextBioi 286356.
    PROi P15535.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P15535.
    Bgeei P15535.
    Genevestigatori P15535.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR003859. Galactosyl_T.
    IPR027791. Galactosyl_T_C.
    IPR027995. Galactosyl_T_N.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    PANTHERi PTHR19300. PTHR19300. 1 hit.
    Pfami PF02709. Glyco_transf_7C. 1 hit.
    PF13733. Glyco_transf_7N. 1 hit.
    [Graphical view ]
    PRINTSi PR02050. B14GALTRFASE.
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the full length cDNA for murine beta-1,4-galactosyltransferase. Novel features at the 5'-end predict two translational start sites at two in-frame AUGs."
      Shaper N.L., Hollis G.F., Douglas J.G., Kirsch I.R., Shaper J.H.
      J. Biol. Chem. 263:10420-10428(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
    2. "Murine beta 1,4-galactosyltransferase: both the amounts and structure of the mRNA are regulated during spermatogenesis."
      Shaper N.L., Wright W.W., Sharper J.H.
      Proc. Natl. Acad. Sci. U.S.A. 87:791-795(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Cloning and sequencing of a full-length cDNA of mouse N-acetylglucosamine (beta 1-4)galactosyltransferase."
      Nakazawa K., Ando T., Kimura T., Narimatsu H.
      J. Biochem. 104:165-168(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    6. "Evidence for two forms of murine beta-1,4-galactosyltransferase based on cloning studies."
      Shaper J.H., Hollis G.F., Shaper N.L.
      Biochimie 70:1683-1688(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE OF 1-63.
    7. "Murine beta 1,4-galactosyltransferase: round spermatid transcripts are characterized by an extended 5'-untranslated region."
      Harduin-Lepers A., Shaper N.L., Mahoney J.A., Shaper J.H.
      Glycobiology 2:361-368(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE OF 1-20.
      Strain: BALB/c.
    8. "Characterization of two cis-regulatory regions in the murine beta 1,4-galactosyltransferase gene. Evidence for a negative regulatory element that controls initiation at the proximal site."
      Harduin-Lepers A., Shaper J.H., Shaper N.L.
      J. Biol. Chem. 268:14348-14359(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-20.
      Strain: BALB/c.
    9. "Differential gene expression of beta-1,4-galactosyltransferases I, II and V during mouse brain development."
      Nakamura N., Yamakawa N., Sato T., Tojo H., Tachi C., Furukawa K.
      J. Neurochem. 76:29-38(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.
      Strain: BALB/c.
      Tissue: Brain.

    Entry informationi

    Entry nameiB4GT1_MOUSE
    AccessioniPrimary (citable) accession number: P15535
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: April 1, 1990
    Last modified: October 1, 2014
    This is version 153 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3