Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Tripartite motif-containing protein 30A

Gene

Trim30a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Trans-acting factor that regulates gene expression of interleukin 2 receptor alpha chain. May affect IL2R-alpha expression through cis-acting negative regulatory elements or through competition with proteins that bind to enhancer or activator sequences. Negatively regulates Toll-like receptor (TLR)-mediated activation of NFKB by promoting degradation of TAB2 and TAB3 and preventing TRAF6 autoubiquitination. Negatively regulates production of reactive oxygen species (ROS) which inhibits activation of the NLRP3 inflammasome complex. This, in turn, regulates activation of CASP1 and subsequent cleavage of IL1B and IL18. No activity detected against a range of retroviruses including a number of lentiviruses, gammaretroviruses and betaretroviruses.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri15 – 5945RING-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri91 – 13242B box-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • negative regulation of interleukin-6 production Source: UniProtKB
  • negative regulation of NLRP3 inflammasome complex assembly Source: UniProtKB
  • negative regulation of reactive oxygen species metabolic process Source: UniProtKB
  • negative regulation of toll-like receptor signaling pathway Source: UniProtKB
  • negative regulation of tumor necrosis factor production Source: UniProtKB
  • positive regulation of protein catabolic process Source: UniProtKB
  • positive regulation of viral entry into host cell Source: UniProtKB
  • protein autoubiquitination Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Tripartite motif-containing protein 30A
Alternative name(s):
Down regulatory protein of interleukin-2 receptor
Tripartite motif-containing protein 30
Gene namesi
Name:Trim30a
Synonyms:Rpt-1, Rpt1, Trim30
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:98178. Trim30a.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi35 – 351C → S: Reduced interaction with NR2C2 and enhanced interaction with TAB2 and TAB3. Does not decrease TAB2 or TAB3 expression. No effect on inhibition of ROS or negative regulation of NLRP3 inflammasome activation. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 496496Tripartite motif-containing protein 30APRO_0000056244Add
BLAST

Proteomic databases

EPDiP15533.
PaxDbiP15533.
PRIDEiP15533.

PTM databases

iPTMnetiP15533.
PhosphoSiteiP15533.

Expressioni

Tissue specificityi

Highly expressed in spleen and lymph nodes (at protein level).1 Publication

Inductioni

By the TLR ligands lipopolysaccharide, CpG dinucleotide and polyinosinic-polycytidylic acid.1 Publication

Gene expression databases

BgeeiENSMUSG00000030921.
CleanExiMM_TRIM30.
GenevisibleiP15533. MM.

Interactioni

Subunit structurei

Homomultimer. Interacts with NR2C2/TAK1, TAB2 and TAB3. Does not interact with NLRP3, NLRC4 or TAB1.2 Publications

Protein-protein interaction databases

BioGridi203018. 6 interactions.
IntActiP15533. 2 interactions.
STRINGi10090.ENSMUSP00000076189.

Structurei

Secondary structure

1
496
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni11 – 133Combined sources
Turni16 – 183Combined sources
Helixi36 – 4510Combined sources
Turni56 – 583Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ECWNMR-A1-78[»]
ProteinModelPortaliP15533.
SMRiP15533. Positions 5-78, 89-494.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15533.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini281 – 496216B30.2/SPRYPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni205 – 2106Highly hydrophilic

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili173 – 23967Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi268 – 2769Nuclear localization signalSequence analysis

Sequence similaritiesi

Contains 1 B box-type zinc finger.PROSITE-ProRule annotation
Contains 1 B30.2/SPRY domain.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri15 – 5945RING-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri91 – 13242B box-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiENOG410ITFH. Eukaryota.
ENOG410ZW0W. LUCA.
GeneTreeiENSGT00760000118893.
HOGENOMiHOG000234134.
HOVERGENiHBG001357.
InParanoidiP15533.
OrthoDBiEOG091G050D.
PhylomeDBiP15533.
TreeFamiTF342569.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
4.10.45.10. 1 hit.
InterProiIPR001870. B30.2/SPRY.
IPR003879. Butyrophylin.
IPR013320. ConA-like_dom.
IPR003877. SPRY_dom.
IPR027370. Znf-RING_LisH.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00622. SPRY. 1 hit.
PF00643. zf-B_box. 1 hit.
PF13445. zf-RING_UBOX. 1 hit.
[Graphical view]
PRINTSiPR01407. BUTYPHLNCDUF.
SMARTiSM00336. BBOX. 1 hit.
SM00184. RING. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Alpha (identifier: P15533-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASSVLEMIK EEVTCPICLE LLKEPVSADC NHSFCRACIT LNYESNRNTD
60 70 80 90 100
GKGNCPVCRV PYPFGNLRPN LHVANIVERL KGFKSIPEEE QKVNICAQHG
110 120 130 140 150
EKLRLFCRKD MMVICWLCER SQEHRGHQTA LIEEVDQEYK EKLQGALWKL
160 170 180 190 200
MKKAKICDEW QDDLQLQRVD WENQIQINVE NVQRQFKGLR DLLDSKENEE
210 220 230 240 250
LQKLKKEKKE VMEKLEESEN ELEDQTELVR DLISDVEHHL ELSTLEMLQG
260 270 280 290 300
ANCVLRRSQS LSLQQPQTVP QKRKRTFQAP DLKGMLQVYQ GLMDIQQYWV
310 320 330 340 350
HMTLHARNNA VIAINKEKRQ IQYRSYNTVP VSEIYHLGVL GYPALSSGKH
360 370 380 390 400
YWEVDISRSD AWLLGLNDGK CAQPQLHSKE EMGIKKNLHS QIKQNVLFQP
410 420 430 440 450
KCGYWVIGMK NPSVYKAFDE CSITHNSSIL VISLPDRPSR VGVFLDRKAG
460 470 480 490
TLSFYDVSNC GALIYRFYDP AFPVEVYPYF NPMKCSEPMT ICGPPS
Length:496
Mass (Da):57,330
Last modified:February 11, 2002 - v2
Checksum:i66502B93BDC3A1D2
GO
Isoform Beta (identifier: P15533-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     145-151: GALWKLM → SLHHTEL
     152-496: Missing.

Show »
Length:151
Mass (Da):17,414
Checksum:i02DDB23DC94FB7D9
GO

Sequence cautioni

The sequence AAA40073 differs from that shown. Reason: Frameshift at position 319. Curated
The sequence AAG53470 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti68 – 681R → K in AAH05447 (PubMed:15489334).Curated
Sequence conflicti148 – 1481W → R in AAH05447 (PubMed:15489334).Curated
Sequence conflicti241 – 2411E → Q in AAH05447 (PubMed:15489334).Curated
Sequence conflicti268 – 2681T → S in AAH05447 (PubMed:15489334).Curated
Sequence conflicti416 – 4161K → N in AAH05447 (PubMed:15489334).Curated
Sequence conflicti437 – 4371R → C in AAH05447 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei145 – 1517GALWKLM → SLHHTEL in isoform Beta. 1 PublicationVSP_005762
Alternative sequencei152 – 496345Missing in isoform Beta. 1 PublicationVSP_005763Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03776 mRNA. Translation: AAA40073.1. Frameshift.
AF220014 mRNA. Translation: AAG53468.1.
AF220015 mRNA. Translation: AAG53469.1.
AF220016 Genomic DNA. Translation: AAG53470.1. Different initiation.
AK134144 mRNA. Translation: BAE22032.1.
AK137506 mRNA. Translation: BAE23387.1.
AC122400 Genomic DNA. No translation available.
AC142110 Genomic DNA. No translation available.
CH466531 Genomic DNA. Translation: EDL16725.1.
CH466531 Genomic DNA. Translation: EDL16726.1.
BC005447 mRNA. Translation: AAH05447.1.
CCDSiCCDS40068.1. [P15533-1]
PIRiA30891.
RefSeqiNP_033125.2. NM_009099.2. [P15533-1]
XP_006507534.1. XM_006507471.1. [P15533-1]
UniGeneiMm.295578.

Genome annotation databases

EnsembliENSMUST00000076922; ENSMUSP00000076189; ENSMUSG00000030921. [P15533-1]
GeneIDi20128.
KEGGimmu:20128.
UCSCiuc009iwh.1. mouse. [P15533-1]
uc009iwi.1. mouse. [P15533-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03776 mRNA. Translation: AAA40073.1. Frameshift.
AF220014 mRNA. Translation: AAG53468.1.
AF220015 mRNA. Translation: AAG53469.1.
AF220016 Genomic DNA. Translation: AAG53470.1. Different initiation.
AK134144 mRNA. Translation: BAE22032.1.
AK137506 mRNA. Translation: BAE23387.1.
AC122400 Genomic DNA. No translation available.
AC142110 Genomic DNA. No translation available.
CH466531 Genomic DNA. Translation: EDL16725.1.
CH466531 Genomic DNA. Translation: EDL16726.1.
BC005447 mRNA. Translation: AAH05447.1.
CCDSiCCDS40068.1. [P15533-1]
PIRiA30891.
RefSeqiNP_033125.2. NM_009099.2. [P15533-1]
XP_006507534.1. XM_006507471.1. [P15533-1]
UniGeneiMm.295578.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ECWNMR-A1-78[»]
ProteinModelPortaliP15533.
SMRiP15533. Positions 5-78, 89-494.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203018. 6 interactions.
IntActiP15533. 2 interactions.
STRINGi10090.ENSMUSP00000076189.

PTM databases

iPTMnetiP15533.
PhosphoSiteiP15533.

Proteomic databases

EPDiP15533.
PaxDbiP15533.
PRIDEiP15533.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000076922; ENSMUSP00000076189; ENSMUSG00000030921. [P15533-1]
GeneIDi20128.
KEGGimmu:20128.
UCSCiuc009iwh.1. mouse. [P15533-1]
uc009iwi.1. mouse. [P15533-2]

Organism-specific databases

CTDi20128.
MGIiMGI:98178. Trim30a.

Phylogenomic databases

eggNOGiENOG410ITFH. Eukaryota.
ENOG410ZW0W. LUCA.
GeneTreeiENSGT00760000118893.
HOGENOMiHOG000234134.
HOVERGENiHBG001357.
InParanoidiP15533.
OrthoDBiEOG091G050D.
PhylomeDBiP15533.
TreeFamiTF342569.

Miscellaneous databases

EvolutionaryTraceiP15533.
PROiP15533.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030921.
CleanExiMM_TRIM30.
GenevisibleiP15533. MM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
4.10.45.10. 1 hit.
InterProiIPR001870. B30.2/SPRY.
IPR003879. Butyrophylin.
IPR013320. ConA-like_dom.
IPR003877. SPRY_dom.
IPR027370. Znf-RING_LisH.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00622. SPRY. 1 hit.
PF00643. zf-B_box. 1 hit.
PF13445. zf-RING_UBOX. 1 hit.
[Graphical view]
PRINTSiPR01407. BUTYPHLNCDUF.
SMARTiSM00336. BBOX. 1 hit.
SM00184. RING. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTR30A_MOUSE
AccessioniPrimary (citable) accession number: P15533
Secondary accession number(s): Q3UV85
, Q3UZ29, Q99K55, Q99PQ7, Q99PQ8, Q99PQ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: February 11, 2002
Last modified: September 7, 2016
This is version 164 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.