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P15532 (NDKA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 136. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nucleoside diphosphate kinase A

Short name=NDK A
Short name=NDP kinase A
EC=2.7.4.6
Alternative name(s):
Metastasis inhibition factor NM23
NDPK-A
Tumor metastatic process-associated protein
nm23-M1
Gene names
Name:Nme1
Synonyms:Nm23
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length152 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair By similarity. HAMAP-Rule MF_00451

Catalytic activity

ATP + nucleoside diphosphate = ADP + nucleoside triphosphate. HAMAP-Rule MF_00451

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00451

Enzyme regulation

Autophosphorylation at His-118 increases serine/threonine protein kinase activity of the enzyme. Interaction with the SET complex inhibits exonuclease activity By similarity. HAMAP-Rule MF_00451

Subunit structure

Hexamer of two different chains: A and B (A6, A5B, A4B2, A3B3, A2B4, AB5, B6). Interacts with PRUNE. Component of the SET complex, composed of at least ANP32A, APEX1, HMGB2, NME1, SET and TREX1. Within this complex, interacts directly with SET. Also interacts with TREX1, but only following translocation to the nucleus By similarity.

Subcellular location

Cytoplasm. Nucleus HAMAP-Rule MF_00451.

Involvement in disease

This protein is found in reduced amount in tumor cells of high metastatic potential.

Sequence similarities

Belongs to the NDK family.

Sequence caution

The sequence AAA39826.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Biological processDifferentiation
Endocytosis
Neurogenesis
Nucleotide metabolism
   Cellular componentCytoplasm
Nucleus
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   PTMAcetylation
Isopeptide bond
Ubl conjugation
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processCTP biosynthetic process

Inferred from electronic annotation. Source: InterPro

GTP biosynthetic process

Inferred from electronic annotation. Source: InterPro

UTP biosynthetic process

Inferred from electronic annotation. Source: InterPro

cellular response to drug

Inferred from electronic annotation. Source: Ensembl

cellular response to fatty acid

Inferred from electronic annotation. Source: Ensembl

cellular response to glucose stimulus

Inferred from electronic annotation. Source: Ensembl

endocytosis

Inferred from electronic annotation. Source: UniProtKB-KW

hippocampus development

Inferred from electronic annotation. Source: Ensembl

lactation

Inferred from mutant phenotype PubMed 12848338. Source: MGI

mammary gland development

Inferred from mutant phenotype PubMed 12848338. Source: MGI

negative regulation of gene expression

Inferred from electronic annotation. Source: Ensembl

negative regulation of myeloid leukocyte differentiation

Inferred from electronic annotation. Source: Ensembl

positive regulation of DNA binding

Inferred from electronic annotation. Source: Ensembl

positive regulation of epithelial cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of neuron projection development

Inferred from electronic annotation. Source: Ensembl

response to amine

Inferred from electronic annotation. Source: Ensembl

response to cAMP

Inferred from electronic annotation. Source: Ensembl

response to testosterone

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcentrosome

Inferred from electronic annotation. Source: Ensembl

cytosol

Inferred from electronic annotation. Source: Ensembl

mitochondrion

Inferred from direct assay PubMed 18614015. Source: MGI

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

perinuclear region of cytoplasm

Inferred from electronic annotation. Source: Ensembl

ruffle membrane

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTP binding

Inferred from electronic annotation. Source: Ensembl

RNA polymerase II regulatory region sequence-specific DNA binding

Inferred from electronic annotation. Source: Ensembl

deoxyribonuclease activity

Inferred from electronic annotation. Source: Ensembl

magnesium ion binding

Inferred from electronic annotation. Source: Ensembl

nucleoside diphosphate kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

ribosomal small subunit binding

Inferred from electronic annotation. Source: Ensembl

single-stranded DNA binding

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 152151Nucleoside diphosphate kinase A HAMAP-Rule MF_00451
PRO_0000137115

Sites

Active site1181Pros-phosphohistidine intermediate
Binding site121ATP By similarity
Binding site601ATP By similarity
Binding site881ATP By similarity
Binding site941ATP By similarity
Binding site1051ATP By similarity
Binding site1151ATP By similarity

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue1241N6-acetyllysine Ref.8
Cross-link100Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Sequences

Sequence LengthMass (Da)Tools
P15532 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: EE2E4DB218024686

FASTA15217,208
        10         20         30         40         50         60 
MANSERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVGLKF LQASEDLLKE HYTDLKDRPF 

        70         80         90        100        110        120 
FTGLVKYMHS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS 

       130        140        150 
DSVKSAEKEI SLWFQPEELV EYKSCAQNWI YE 

« Hide

References

« Hide 'large scale' references
[1]"Reduced Nm23/Awd protein in tumour metastasis and aberrant Drosophila development."
Rosengard A.M., Krutzsch H.C., Shearn A., Biggs J.R., Barker E., Margulies I.M.K., King C.R., Liotta L.A., Steeg P.S.
Nature 342:177-180(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Evidence for a novel gene associated with low tumor metastatic potential."
Steeg P.S., Bevilacqua G., Kopper L., Thorgeirsson U.P., Talmadge J.E., Liotta L.A., Sobel M.E.
J. Natl. Cancer Inst. 80:200-204(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Reduced tumor incidence, metastatic potential, and cytokine responsiveness of nm23-transfected melanoma cells."
Leone A., Flatow U., King C.R., Sandeen M.A., Margulies I.M., Liotta L.A., Steeg P.S.
Cell 65:25-35(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]Dabernat S., Masse K., Daniel J.Y.
Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: 129/Sv.
[5]Gervasi F., Fanciulli M., Lombardi D.
Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Swiss Webster / NIH.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary gland.
[7]Lubec G., Klug S., Kang S.U.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 7-26; 67-85; 89-124 AND 129-143, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: C57BL/6.
Tissue: Brain and Hippocampus.
[8]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-124, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M35970 mRNA. Translation: AAA39826.1. Different initiation.
M65037 mRNA. Translation: AAA63391.1.
U85511 mRNA. Translation: AAB42080.1.
AF033377 mRNA. Translation: AAB87689.1.
BC005629 mRNA. Translation: AAH05629.1.
CCDSCCDS25247.1.
PIRA46557.
RefSeqNP_032730.1. NM_008704.2.
UniGeneMm.439702.

3D structure databases

ProteinModelPortalP15532.
SMRP15532. Positions 5-152.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid201788. 2 interactions.
IntActP15532. 5 interactions.
MINTMINT-1868955.

PTM databases

PhosphoSiteP15532.

2D gel databases

REPRODUCTION-2DPAGEP15532.
SWISS-2DPAGEP15532.

Proteomic databases

MaxQBP15532.
PaxDbP15532.
PRIDEP15532.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000135884; ENSMUSP00000117022; ENSMUSG00000037601.
GeneID18102.
KEGGmmu:18102.
UCSCuc007kxu.1. mouse.

Organism-specific databases

CTD4830.
MGIMGI:97355. Nme1.

Phylogenomic databases

eggNOGCOG0105.
HOGENOMHOG000224564.
HOVERGENHBG000423.
InParanoidP15532.
KOK00940.
OMAVWEGDNI.
OrthoDBEOG7GJ6FG.
PhylomeDBP15532.
TreeFamTF106373.

Gene expression databases

ArrayExpressP15532.
BgeeP15532.
GenevestigatorP15532.

Family and domain databases

Gene3D3.30.70.141. 1 hit.
HAMAPMF_00451. NDP_kinase.
InterProIPR001564. Nucleoside_diP_kinase.
IPR023005. Nucleoside_diP_kinase_AS.
[Graphical view]
PfamPF00334. NDK. 1 hit.
[Graphical view]
PRINTSPR01243. NUCDPKINASE.
SMARTSM00562. NDK. 1 hit.
[Graphical view]
SUPFAMSSF54919. SSF54919. 1 hit.
PROSITEPS00469. NDP_KINASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNME1. mouse.
NextBio293271.
PROP15532.
SOURCESearch...

Entry information

Entry nameNDKA_MOUSE
AccessionPrimary (citable) accession number: P15532
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: July 9, 2014
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot