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P15532 (NDKA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Nucleoside diphosphate kinase A

Short name=NDK A
Short name=NDP kinase A
EC=2.7.4.6
Alternative name(s):
Metastasis inhibition factor NM23
NDPK-A
Tumor metastatic process-associated protein
nm23-M1
Gene names
Name:Nme1
Synonyms:Nm23
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length152 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination By similarity.

Catalytic activity

ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.

Cofactor

Magnesium By similarity.

Enzyme regulation

Autophosphorylation at His-118 increases serine/threonine protein kinase activity of the enzyme. Interaction with the SET complex inhibits exonuclease activity By similarity.

Subunit structure

Hexamer of two different chains: A and B (A6, A5B, A4B2, A3B3, A2B4, AB5, B6). Interacts with SET and PRUNE By similarity.

Subcellular location

Cytoplasm. Nucleus.

Involvement in disease

Note=This protein is found in reduced amount in tumor cells of high metastatic potential.

Sequence similarities

Belongs to the NDK family.

Sequence caution

The sequence AAA39826.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 152152Nucleoside diphosphate kinase A
PRO_0000137115

Sites

Active site1181Pros-phosphohistidine intermediate
Binding site121ATP By similarity
Binding site601ATP By similarity
Binding site881ATP By similarity
Binding site941ATP By similarity
Binding site1051ATP By similarity
Binding site1151ATP By similarity

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue521Phosphotyrosine By similarity
Modified residue561N6-acetyllysine By similarity
Modified residue941Phosphothreonine By similarity
Cross-link100Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Sequences

Sequence LengthMass (Da)Tools
P15532 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: EE2E4DB218024686

FASTA15217,208
        10         20         30         40         50         60 
MANSERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVGLKF LQASEDLLKE HYTDLKDRPF 

        70         80         90        100        110        120 
FTGLVKYMHS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS 

       130        140        150 
DSVKSAEKEI SLWFQPEELV EYKSCAQNWI YE 

« Hide

References

« Hide 'large scale' references
[1]"Reduced Nm23/Awd protein in tumour metastasis and aberrant Drosophila development."
Rosengard A.M., Krutzsch H.C., Shearn A., Biggs J.R., Barker E., Margulies I.M.K., King C.R., Liotta L.A., Steeg P.S.
Nature 342:177-180(1989) [PubMed: 2509941] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Evidence for a novel gene associated with low tumor metastatic potential."
Steeg P.S., Bevilacqua G., Kopper L., Thorgeirsson U.P., Talmadge J.E., Liotta L.A., Sobel M.E.
J. Natl. Cancer Inst. 80:200-204(1988) [PubMed: 3346912] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Reduced tumor incidence, metastatic potential, and cytokine responsiveness of nm23-transfected melanoma cells."
Leone A., Flatow U., King C.R., Sandeen M.A., Margulies I.M., Liotta L.A., Steeg P.S.
Cell 65:25-35(1991) [PubMed: 2013093] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]Dabernat S., Masse K., Daniel J.Y.
Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: 129/Sv.
[5]Gervasi F., Fanciulli M., Lombardi D.
Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Swiss Webster / NIH.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary gland.
[7]Lubec G., Klug S., Kang S.U.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 7-26; 67-85; 89-124 AND 129-143, MASS SPECTROMETRY.
Strain: C57BL/6.
Tissue: Brain and Hippocampus.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M35970 mRNA. Translation: AAA39826.1. Different initiation.
M65037 mRNA. Translation: AAA63391.1.
U85511 mRNA. Translation: AAB42080.1.
AF033377 mRNA. Translation: AAB87689.1.
BC005629 mRNA. Translation: AAH05629.1.
IPIIPI00131459.
PIRA46557.
RefSeqNP_032730.1. NM_008704.2.
UniGeneMm.439702.

3D structure databases

ProteinModelPortalP15532.
SMRP15532. Positions 5-152.
ModBaseSearch...

Protein-protein interaction databases

IntActP15532. 2 interactions.
STRINGP15532.

PTM databases

PhosphoSiteP15532.

2D gel databases

SWISS-2DPAGEP15532.
REPRODUCTION-2DPAGEP15532.

Proteomic databases

PRIDEP15532.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000135884; ENSMUSP00000117022; ENSMUSG00000037601.
GeneID18102.
KEGGmmu:18102.

Organism-specific databases

CTD4830.
MGIMGI:97355. Nme1.

Phylogenomic databases

eggNOGmaNOG10260.
HOGENOMHBG445152.
HOVERGENHBG000423.
InParanoidP15532.
OMAKSCAQNW.
PhylomeDBP15532.

Gene expression databases

ArrayExpressP15532.
BgeeP15532.
GenevestigatorP15532.
GermOnlineENSMUSG00000037601. Mus musculus.

Family and domain databases

InterProIPR001564. Nucleoside_diP_kinase.
IPR023005. Nucleoside_diP_kinase_AS.
[Graphical view]
Gene3DG3DSA:3.30.70.141. NDK. 1 hit.
KOK00940.
PANTHERPTHR11349. Nuc_diP_kinase_core. 1 hit.
PfamPF00334. NDK. 1 hit.
[Graphical view]
PRINTSPR01243. NUCDPKINASE.
SMARTSM00562. NDK. 1 hit.
[Graphical view]
SUPFAMSSF54919. NDK. 1 hit.
PROSITEPS00469. NDP_KINASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio459792.
SOURCESearch...

Entry information

Entry nameNDKA_MOUSE
AccessionPrimary (citable) accession number: P15532
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: November 16, 2011
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families