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P15532

- NDKA_MOUSE

UniProt

P15532 - NDKA_MOUSE

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Protein

Nucleoside diphosphate kinase A

Gene
Nme1, Nm23
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair By similarity.UniRule annotation

Catalytic activityi

ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Autophosphorylation at His-118 increases serine/threonine protein kinase activity of the enzyme. Interaction with the SET complex inhibits exonuclease activity By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei12 – 121ATP By similarity
Binding sitei60 – 601ATP By similarity
Binding sitei88 – 881ATP By similarity
Binding sitei94 – 941ATP By similarity
Binding sitei105 – 1051ATP By similarity
Binding sitei115 – 1151ATP By similarity
Active sitei118 – 1181Pros-phosphohistidine intermediate

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. deoxyribonuclease activity Source: Ensembl
  3. GTP binding Source: Ensembl
  4. magnesium ion binding Source: Ensembl
  5. nucleoside diphosphate kinase activity Source: UniProtKB-EC
  6. ribosomal small subunit binding Source: Ensembl
  7. RNA polymerase II regulatory region sequence-specific DNA binding Source: Ensembl
  8. single-stranded DNA binding Source: Ensembl

GO - Biological processi

  1. cellular response to drug Source: Ensembl
  2. cellular response to fatty acid Source: Ensembl
  3. cellular response to glucose stimulus Source: Ensembl
  4. CTP biosynthetic process Source: InterPro
  5. endocytosis Source: UniProtKB-KW
  6. GTP biosynthetic process Source: InterPro
  7. hippocampus development Source: Ensembl
  8. lactation Source: MGI
  9. mammary gland development Source: MGI
  10. negative regulation of gene expression Source: Ensembl
  11. negative regulation of myeloid leukocyte differentiation Source: Ensembl
  12. positive regulation of DNA binding Source: Ensembl
  13. positive regulation of epithelial cell proliferation Source: Ensembl
  14. positive regulation of neuron projection development Source: Ensembl
  15. response to amine Source: Ensembl
  16. response to cAMP Source: Ensembl
  17. response to testosterone Source: Ensembl
  18. UTP biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Differentiation, Endocytosis, Neurogenesis, Nucleotide metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoside diphosphate kinase A (EC:2.7.4.6)
Short name:
NDK A
Short name:
NDP kinase A
Alternative name(s):
Metastasis inhibition factor NM23
NDPK-A
Tumor metastatic process-associated protein
nm23-M1
Gene namesi
Name:Nme1
Synonyms:Nm23
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:97355. Nme1.

Subcellular locationi

GO - Cellular componenti

  1. centrosome Source: Ensembl
  2. cytosol Source: Ensembl
  3. mitochondrion Source: MGI
  4. nucleus Source: UniProtKB-SubCell
  5. perinuclear region of cytoplasm Source: Ensembl
  6. ruffle membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Involvement in diseasei

This protein is found in reduced amount in tumor cells of high metastatic potential.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 152151Nucleoside diphosphate kinase AUniRule annotationPRO_0000137115Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine By similarity
Cross-linki100 – 100Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Modified residuei124 – 1241N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP15532.
PaxDbiP15532.
PRIDEiP15532.

2D gel databases

REPRODUCTION-2DPAGEP15532.
SWISS-2DPAGEP15532.

PTM databases

PhosphoSiteiP15532.

Expressioni

Gene expression databases

ArrayExpressiP15532.
BgeeiP15532.
GenevestigatoriP15532.

Interactioni

Subunit structurei

Hexamer of two different chains: A and B (A6, A5B, A4B2, A3B3, A2B4, AB5, B6). Interacts with PRUNE. Component of the SET complex, composed of at least ANP32A, APEX1, HMGB2, NME1, SET and TREX1. Within this complex, interacts directly with SET. Also interacts with TREX1, but only following translocation to the nucleus By similarity.

Protein-protein interaction databases

BioGridi201788. 2 interactions.
IntActiP15532. 5 interactions.
MINTiMINT-1868955.

Structurei

3D structure databases

ProteinModelPortaliP15532.
SMRiP15532. Positions 5-152.

Family & Domainsi

Sequence similaritiesi

Belongs to the NDK family.

Phylogenomic databases

eggNOGiCOG0105.
HOGENOMiHOG000224564.
HOVERGENiHBG000423.
InParanoidiP15532.
KOiK00940.
OMAiVWEGDNI.
OrthoDBiEOG7GJ6FG.
PhylomeDBiP15532.
TreeFamiTF106373.

Family and domain databases

Gene3Di3.30.70.141. 1 hit.
HAMAPiMF_00451. NDP_kinase.
InterProiIPR001564. Nucleoside_diP_kinase.
IPR023005. Nucleoside_diP_kinase_AS.
[Graphical view]
PfamiPF00334. NDK. 1 hit.
[Graphical view]
PRINTSiPR01243. NUCDPKINASE.
SMARTiSM00562. NDK. 1 hit.
[Graphical view]
SUPFAMiSSF54919. SSF54919. 1 hit.
PROSITEiPS00469. NDP_KINASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15532-1 [UniParc]FASTAAdd to Basket

« Hide

MANSERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVGLKF LQASEDLLKE    50
HYTDLKDRPF FTGLVKYMHS GPVVAMVWEG LNVVKTGRVM LGETNPADSK 100
PGTIRGDFCI QVGRNIIHGS DSVKSAEKEI SLWFQPEELV EYKSCAQNWI 150
YE 152
Length:152
Mass (Da):17,208
Last modified:April 1, 1990 - v1
Checksum:iEE2E4DB218024686
GO

Sequence cautioni

The sequence AAA39826.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M35970 mRNA. Translation: AAA39826.1. Different initiation.
M65037 mRNA. Translation: AAA63391.1.
U85511 mRNA. Translation: AAB42080.1.
AF033377 mRNA. Translation: AAB87689.1.
BC005629 mRNA. Translation: AAH05629.1.
CCDSiCCDS25247.1.
PIRiA46557.
RefSeqiNP_032730.1. NM_008704.2.
UniGeneiMm.439702.

Genome annotation databases

EnsembliENSMUST00000135884; ENSMUSP00000117022; ENSMUSG00000037601.
GeneIDi18102.
KEGGimmu:18102.
UCSCiuc007kxu.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M35970 mRNA. Translation: AAA39826.1 . Different initiation.
M65037 mRNA. Translation: AAA63391.1 .
U85511 mRNA. Translation: AAB42080.1 .
AF033377 mRNA. Translation: AAB87689.1 .
BC005629 mRNA. Translation: AAH05629.1 .
CCDSi CCDS25247.1.
PIRi A46557.
RefSeqi NP_032730.1. NM_008704.2.
UniGenei Mm.439702.

3D structure databases

ProteinModelPortali P15532.
SMRi P15532. Positions 5-152.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 201788. 2 interactions.
IntActi P15532. 5 interactions.
MINTi MINT-1868955.

PTM databases

PhosphoSitei P15532.

2D gel databases

REPRODUCTION-2DPAGE P15532.
SWISS-2DPAGE P15532.

Proteomic databases

MaxQBi P15532.
PaxDbi P15532.
PRIDEi P15532.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000135884 ; ENSMUSP00000117022 ; ENSMUSG00000037601 .
GeneIDi 18102.
KEGGi mmu:18102.
UCSCi uc007kxu.1. mouse.

Organism-specific databases

CTDi 4830.
MGIi MGI:97355. Nme1.

Phylogenomic databases

eggNOGi COG0105.
HOGENOMi HOG000224564.
HOVERGENi HBG000423.
InParanoidi P15532.
KOi K00940.
OMAi VWEGDNI.
OrthoDBi EOG7GJ6FG.
PhylomeDBi P15532.
TreeFami TF106373.

Miscellaneous databases

ChiTaRSi NME1. mouse.
NextBioi 293271.
PROi P15532.
SOURCEi Search...

Gene expression databases

ArrayExpressi P15532.
Bgeei P15532.
Genevestigatori P15532.

Family and domain databases

Gene3Di 3.30.70.141. 1 hit.
HAMAPi MF_00451. NDP_kinase.
InterProi IPR001564. Nucleoside_diP_kinase.
IPR023005. Nucleoside_diP_kinase_AS.
[Graphical view ]
Pfami PF00334. NDK. 1 hit.
[Graphical view ]
PRINTSi PR01243. NUCDPKINASE.
SMARTi SM00562. NDK. 1 hit.
[Graphical view ]
SUPFAMi SSF54919. SSF54919. 1 hit.
PROSITEi PS00469. NDP_KINASES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Reduced Nm23/Awd protein in tumour metastasis and aberrant Drosophila development."
    Rosengard A.M., Krutzsch H.C., Shearn A., Biggs J.R., Barker E., Margulies I.M.K., King C.R., Liotta L.A., Steeg P.S.
    Nature 342:177-180(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Reduced tumor incidence, metastatic potential, and cytokine responsiveness of nm23-transfected melanoma cells."
    Leone A., Flatow U., King C.R., Sandeen M.A., Margulies I.M., Liotta L.A., Steeg P.S.
    Cell 65:25-35(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. Dabernat S., Masse K., Daniel J.Y.
    Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: 129/Sv.
  5. Gervasi F., Fanciulli M., Lombardi D.
    Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Swiss Webster / NIH.
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.
  7. Lubec G., Klug S., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 7-26; 67-85; 89-124 AND 129-143, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain and Hippocampus.
  8. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-124, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiNDKA_MOUSE
AccessioniPrimary (citable) accession number: P15532
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: July 9, 2014
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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