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Protein

Nucleoside diphosphate kinase A

Gene

Nme1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair (By similarity).By similarity

Catalytic activityi

ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.

Cofactori

Mg2+By similarity

Enzyme regulationi

Autophosphorylation at His-118 increases serine/threonine protein kinase activity of the enzyme. Interaction with the SET complex inhibits exonuclease activity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei12ATPBy similarity1
Binding sitei60ATPBy similarity1
Binding sitei88ATPBy similarity1
Binding sitei94ATPBy similarity1
Binding sitei105ATPBy similarity1
Binding sitei115ATPBy similarity1
Active sitei118Pros-phosphohistidine intermediate1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Differentiation, Endocytosis, Neurogenesis, Nucleotide metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-499943. Synthesis and interconversion of nucleotide di- and triphosphates.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoside diphosphate kinase A (EC:2.7.4.6)
Short name:
NDK A
Short name:
NDP kinase A
Alternative name(s):
Metastasis inhibition factor NM23
NDPK-A
Tumor metastatic process-associated protein
nm23-M1
Gene namesi
Name:Nme1
Synonyms:Nm23
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:97355. Nme1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytosol Source: MGI
  • extracellular exosome Source: MGI
  • membrane Source: MGI
  • mitochondrion Source: MGI
  • myelin sheath Source: UniProtKB
  • nucleus Source: MGI
  • ruffle membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Involvement in diseasei

This protein is found in reduced amount in tumor cells of high metastatic potential.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001371152 – 152Nucleoside diphosphate kinase AAdd BLAST151

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki100Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei120PhosphoserineBy similarity1
Modified residuei122PhosphoserineBy similarity1
Modified residuei124N6-acetyllysineCombined sources1
Modified residuei125PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP15532.
PaxDbiP15532.
PeptideAtlasiP15532.
PRIDEiP15532.

2D gel databases

REPRODUCTION-2DPAGEP15532.
SWISS-2DPAGEP15532.

PTM databases

iPTMnetiP15532.
PhosphoSitePlusiP15532.
SwissPalmiP15532.

Expressioni

Gene expression databases

BgeeiENSMUSG00000037601.
ExpressionAtlasiP15532. baseline and differential.
GenevisibleiP15532. MM.

Interactioni

Subunit structurei

Hexamer of two different chains: A and B (A6, A5B, A4B2, A3B3, A2B4, AB5, B6). Interacts with PRUNE. Component of the SET complex, composed of at least ANP32A, APEX1, HMGB2, NME1, SET and TREX1. Within this complex, interacts directly with SET. Also interacts with TREX1, but only following translocation to the nucleus (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201788. 3 interactors.
DIPiDIP-34130N.
IntActiP15532. 6 interactors.
MINTiMINT-1868955.
STRINGi10090.ENSMUSP00000117022.

Structurei

3D structure databases

ProteinModelPortaliP15532.
SMRiP15532.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NDK family.Curated

Phylogenomic databases

eggNOGiKOG0888. Eukaryota.
COG0105. LUCA.
GeneTreeiENSGT00760000119146.
HOGENOMiHOG000224564.
HOVERGENiHBG000423.
InParanoidiP15532.
KOiK00940.
OMAiNASRAWI.
OrthoDBiEOG091G0PLD.
PhylomeDBiP15532.
TreeFamiTF106373.

Family and domain databases

HAMAPiMF_00451. NDP_kinase. 1 hit.
InterProiIPR001564. Nucleoside_diP_kinase.
IPR023005. Nucleoside_diP_kinase_AS.
[Graphical view]
PfamiPF00334. NDK. 1 hit.
[Graphical view]
PRINTSiPR01243. NUCDPKINASE.
SMARTiSM00562. NDK. 1 hit.
[Graphical view]
PROSITEiPS00469. NDP_KINASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15532-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANSERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVGLKF LQASEDLLKE
60 70 80 90 100
HYTDLKDRPF FTGLVKYMHS GPVVAMVWEG LNVVKTGRVM LGETNPADSK
110 120 130 140 150
PGTIRGDFCI QVGRNIIHGS DSVKSAEKEI SLWFQPEELV EYKSCAQNWI

YE
Length:152
Mass (Da):17,208
Last modified:April 1, 1990 - v1
Checksum:iEE2E4DB218024686
GO

Sequence cautioni

The sequence AAA39826 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35970 mRNA. Translation: AAA39826.1. Different initiation.
M65037 mRNA. Translation: AAA63391.1.
U85511 mRNA. Translation: AAB42080.1.
AF033377 mRNA. Translation: AAB87689.1.
BC005629 mRNA. Translation: AAH05629.1.
CCDSiCCDS25247.1.
PIRiA46557.
RefSeqiNP_032730.1. NM_008704.2.
UniGeneiMm.439702.

Genome annotation databases

EnsembliENSMUST00000135884; ENSMUSP00000117022; ENSMUSG00000037601.
GeneIDi18102.
KEGGimmu:18102.
UCSCiuc007kxu.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35970 mRNA. Translation: AAA39826.1. Different initiation.
M65037 mRNA. Translation: AAA63391.1.
U85511 mRNA. Translation: AAB42080.1.
AF033377 mRNA. Translation: AAB87689.1.
BC005629 mRNA. Translation: AAH05629.1.
CCDSiCCDS25247.1.
PIRiA46557.
RefSeqiNP_032730.1. NM_008704.2.
UniGeneiMm.439702.

3D structure databases

ProteinModelPortaliP15532.
SMRiP15532.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201788. 3 interactors.
DIPiDIP-34130N.
IntActiP15532. 6 interactors.
MINTiMINT-1868955.
STRINGi10090.ENSMUSP00000117022.

PTM databases

iPTMnetiP15532.
PhosphoSitePlusiP15532.
SwissPalmiP15532.

2D gel databases

REPRODUCTION-2DPAGEP15532.
SWISS-2DPAGEP15532.

Proteomic databases

EPDiP15532.
PaxDbiP15532.
PeptideAtlasiP15532.
PRIDEiP15532.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000135884; ENSMUSP00000117022; ENSMUSG00000037601.
GeneIDi18102.
KEGGimmu:18102.
UCSCiuc007kxu.1. mouse.

Organism-specific databases

CTDi4830.
MGIiMGI:97355. Nme1.

Phylogenomic databases

eggNOGiKOG0888. Eukaryota.
COG0105. LUCA.
GeneTreeiENSGT00760000119146.
HOGENOMiHOG000224564.
HOVERGENiHBG000423.
InParanoidiP15532.
KOiK00940.
OMAiNASRAWI.
OrthoDBiEOG091G0PLD.
PhylomeDBiP15532.
TreeFamiTF106373.

Enzyme and pathway databases

ReactomeiR-MMU-499943. Synthesis and interconversion of nucleotide di- and triphosphates.

Miscellaneous databases

ChiTaRSiNme1. mouse.
PROiP15532.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000037601.
ExpressionAtlasiP15532. baseline and differential.
GenevisibleiP15532. MM.

Family and domain databases

HAMAPiMF_00451. NDP_kinase. 1 hit.
InterProiIPR001564. Nucleoside_diP_kinase.
IPR023005. Nucleoside_diP_kinase_AS.
[Graphical view]
PfamiPF00334. NDK. 1 hit.
[Graphical view]
PRINTSiPR01243. NUCDPKINASE.
SMARTiSM00562. NDK. 1 hit.
[Graphical view]
PROSITEiPS00469. NDP_KINASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNDKA_MOUSE
AccessioniPrimary (citable) accession number: P15532
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: November 2, 2016
This is version 159 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.