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P15531

- NDKA_HUMAN

UniProt

P15531 - NDKA_HUMAN

Protein

Nucleoside diphosphate kinase A

Gene

NME1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.3 Publications

    Catalytic activityi

    ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.

    Cofactori

    Magnesium.

    Enzyme regulationi

    Autophosphorylation at His-118 increases serine/threonine protein kinase activity of the enzyme. Interaction with the SET complex inhibits the endonuclease activity.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei12 – 121ATP
    Binding sitei60 – 601ATP
    Binding sitei88 – 881ATP
    Binding sitei94 – 941ATP
    Binding sitei105 – 1051ATP
    Binding sitei115 – 1151ATP
    Active sitei118 – 1181Pros-phosphohistidine intermediate1 Publication

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. deoxyribonuclease activity Source: UniProtKB
    3. GTP binding Source: UniProtKB
    4. identical protein binding Source: IntAct
    5. magnesium ion binding Source: UniProtKB
    6. nucleoside diphosphate kinase activity Source: UniProtKB
    7. poly(A) RNA binding Source: UniProtKB
    8. protein binding Source: UniProtKB
    9. ribosomal small subunit binding Source: UniProtKB
    10. RNA polymerase II regulatory region sequence-specific DNA binding Source: Ensembl
    11. single-stranded DNA binding Source: Ensembl

    GO - Biological processi

    1. cellular response to drug Source: Ensembl
    2. cellular response to fatty acid Source: Ensembl
    3. cellular response to glucose stimulus Source: Ensembl
    4. CTP biosynthetic process Source: InterPro
    5. DNA catabolic process Source: GOC
    6. endocytosis Source: UniProtKB-KW
    7. GTP biosynthetic process Source: InterPro
    8. hippocampus development Source: Ensembl
    9. lactation Source: Ensembl
    10. negative regulation of cell proliferation Source: UniProtKB
    11. negative regulation of gene expression Source: Ensembl
    12. negative regulation of myeloid leukocyte differentiation Source: Ensembl
    13. nucleobase-containing small molecule interconversion Source: Reactome
    14. nucleobase-containing small molecule metabolic process Source: Reactome
    15. positive regulation of DNA binding Source: UniProtKB
    16. positive regulation of epithelial cell proliferation Source: HGNC
    17. positive regulation of neuron projection development Source: Ensembl
    18. regulation of apoptotic process Source: UniProtKB
    19. response to amine Source: Ensembl
    20. response to cAMP Source: Ensembl
    21. response to testosterone Source: Ensembl
    22. small molecule metabolic process Source: Reactome
    23. UTP biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Differentiation, Endocytosis, Neurogenesis, Nucleotide metabolism

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:ENSG00000011052-MONOMER.
    ReactomeiREACT_21330. Synthesis and interconversion of nucleotide di- and triphosphates.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nucleoside diphosphate kinase A (EC:2.7.4.6)
    Short name:
    NDK A
    Short name:
    NDP kinase A
    Alternative name(s):
    Granzyme A-activated DNase
    Short name:
    GAAD
    Metastasis inhibition factor nm23
    NM23-H1
    Tumor metastatic process-associated protein
    Gene namesi
    Name:NME1
    Synonyms:NDPKA, NM23
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 17

    Organism-specific databases

    HGNCiHGNC:7849. NME1.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication
    Note: Cell-cycle dependent nuclear localization which can be induced by interaction with Epstein-barr viral proteins or by degradation of the SET complex by GzmA.

    GO - Cellular componenti

    1. centrosome Source: Ensembl
    2. cytoplasm Source: UniProtKB
    3. cytosol Source: UniProtKB
    4. extracellular vesicular exosome Source: UniProt
    5. membrane Source: UniProtKB
    6. mitochondrion Source: Ensembl
    7. nucleus Source: UniProtKB
    8. perinuclear region of cytoplasm Source: Ensembl
    9. ruffle membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi60 – 601F → W: No loss of activity or substrate binding. 1 Publication
    Mutagenesisi96 – 961P → S: Increased motility of carcinoma cells. 1 Publication
    Mutagenesisi118 – 1181H → F: Loss of serine/threonine kinase activity. Some loss of motility of carcinoma cells. 2 Publications
    Mutagenesisi118 – 1181H → G: Loss of activity. 2 Publications
    Mutagenesisi120 – 1201S → A: Limited increase in motility of carcinoma cells. 1 Publication

    Organism-specific databases

    PharmGKBiPA249.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 152151Nucleoside diphosphate kinase APRO_0000137114Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine1 Publication
    Cross-linki100 – 100Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

    Keywords - PTMi

    Acetylation, Isopeptide bond, Ubl conjugation

    Proteomic databases

    MaxQBiP15531.
    PaxDbiP15531.
    PRIDEiP15531.

    2D gel databases

    DOSAC-COBS-2DPAGEP15531.
    OGPiP15531.

    PTM databases

    PhosphoSiteiP15531.

    Expressioni

    Tissue specificityi

    Isoform 1 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen and thymus. Expressed in lung carcinoma cell lines but not in normal lung tissues. Isoform 2 is ubiquitously expressed and its expression is also related to tumor differentiation. Isoform 3 is ubiquitously expressed.3 Publications

    Gene expression databases

    ArrayExpressiP15531.
    BgeeiP15531.
    CleanExiHS_NME1.
    GenevestigatoriP15531.

    Organism-specific databases

    HPAiHPA008467.
    HPA041113.

    Interactioni

    Subunit structurei

    Hexamer of two different chains: A and B (A6, A5B, A4B2, A3B3, A2B4, AB5, B6). Interacts with PRUNE. Component of the SET complex, composed of at least ANP32A, APEX1, HMGB2, NME1, SET and TREX1. Within this complex, interacts directly with SET. Also interacts with TREX1, but only following translocation to the nucleus.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself4EBI-741141,EBI-741141
    Ksr1Q610977EBI-741141,EBI-1536336From a different organism.
    MCF2P109114EBI-741141,EBI-1914514
    NME2P223922EBI-741141,EBI-713693
    PRUNEQ86TP12EBI-741141,EBI-2127112
    STRAPQ9Y3F49EBI-741141,EBI-727414
    WDYHV1Q96HA83EBI-741141,EBI-741158

    Protein-protein interaction databases

    BioGridi110894. 46 interactions.
    DIPiDIP-39164N.
    IntActiP15531. 22 interactions.
    MINTiMINT-221462.
    STRINGi9606.ENSP00000013034.

    Structurei

    Secondary structure

    1
    152
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi2 – 43
    Beta strandi6 – 116
    Helixi13 – 175
    Helixi21 – 3111
    Beta strandi34 – 418
    Helixi45 – 517
    Helixi53 – 553
    Beta strandi56 – 583
    Helixi61 – 699
    Beta strandi73 – 808
    Helixi83 – 919
    Helixi96 – 983
    Helixi104 – 1085
    Helixi112 – 1143
    Beta strandi116 – 1194
    Helixi123 – 13311
    Helixi136 – 1383
    Helixi147 – 1504

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1JXVX-ray2.20A/B/C/D/E/F1-152[»]
    1UCNX-ray2.00A/B/C1-152[»]
    2HVDX-ray2.15A/B/C1-152[»]
    2HVEX-ray2.40A/B/C1-152[»]
    3L7UX-ray2.10A/B/C1-152[»]
    4ENOX-ray2.80A/B1-152[»]
    ProteinModelPortaliP15531.
    SMRiP15531. Positions 1-152.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP15531.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NDK family.Curated

    Phylogenomic databases

    eggNOGiCOG0105.
    HOGENOMiHOG000224564.
    HOVERGENiHBG000423.
    KOiK00940.
    OMAiCAHEWIY.
    OrthoDBiEOG7GJ6FG.
    PhylomeDBiP15531.
    TreeFamiTF106373.

    Family and domain databases

    Gene3Di3.30.70.141. 1 hit.
    HAMAPiMF_00451. NDP_kinase.
    InterProiIPR001564. Nucleoside_diP_kinase.
    IPR023005. Nucleoside_diP_kinase_AS.
    [Graphical view]
    PfamiPF00334. NDK. 1 hit.
    [Graphical view]
    PRINTSiPR01243. NUCDPKINASE.
    SMARTiSM00562. NDK. 1 hit.
    [Graphical view]
    SUPFAMiSSF54919. SSF54919. 1 hit.
    PROSITEiPS00469. NDP_KINASES. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P15531-1) [UniParc]FASTAAdd to Basket

    Also known as: NM23-H1A

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MANCERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVGLKF MQASEDLLKE    50
    HYVDLKDRPF FAGLVKYMHS GPVVAMVWEG LNVVKTGRVM LGETNPADSK 100
    PGTIRGDFCI QVGRNIIHGS DSVESAEKEI GLWFHPEELV DYTSCAQNWI 150
    YE 152
    Length:152
    Mass (Da):17,149
    Last modified:April 1, 1990 - v1
    Checksum:iAAE9C0DF63CB70A1
    GO
    Isoform 2 (identifier: P15531-2) [UniParc]FASTAAdd to Basket

    Also known as: NM23-H1B

    The sequence of this isoform differs from the canonical sequence as follows:
         1-1: M → MVLLSTLGIVFQGEGPPISSCDTGTM

    Show »
    Length:177
    Mass (Da):19,654
    Checksum:iDEA9961E992D0378
    GO
    Isoform 3 (identifier: P22392-2) [UniParc]FASTAAdd to Basket

    Also known as: NM23-LV

    The sequence of this isoform can be found in the external entry P22392.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

    Note: Based on a naturally occurring readthrough transcript which produces an NME1-NME2 fusion protein.

    Length:267
    Mass (Da):30,137
    GO

    Sequence cautioni

    The sequence CAA35621.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti120 – 1201S → G in a neuroblastoma sample; increased motility of carcinoma cells. 1 Publication
    Corresponds to variant rs121917887 [ dbSNP | Ensembl ].
    VAR_004625

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 11M → MVLLSTLGIVFQGEGPPISS CDTGTM in isoform 2. 1 PublicationVSP_036707

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17620 mRNA. Translation: CAA35621.1. Different initiation.
    X73066 mRNA. Translation: CAA51527.1.
    X75598 Genomic DNA. Translation: CAA53270.1.
    AF487339 mRNA. Translation: AAO85436.1.
    AK291105 mRNA. Translation: BAF83794.1.
    CR542104 mRNA. Translation: CAG46901.1.
    CR542115 mRNA. Translation: CAG46912.1.
    AC005839 Genomic DNA. No translation available.
    CH471109 Genomic DNA. Translation: EAW94568.1.
    BC000293 mRNA. Translation: AAH00293.1.
    BC018994 mRNA. Translation: AAH18994.1.
    CCDSiCCDS11578.1. [P15531-2]
    CCDS11579.1. [P15531-1]
    PIRiA33386.
    RefSeqiNP_000260.1. NM_000269.2. [P15531-1]
    NP_937818.1. NM_198175.1. [P15531-2]
    UniGeneiHs.463456.

    Genome annotation databases

    EnsembliENST00000013034; ENSP00000013034; ENSG00000239672. [P15531-2]
    ENST00000336097; ENSP00000337060; ENSG00000239672. [P15531-2]
    ENST00000393196; ENSP00000376892; ENSG00000239672. [P15531-1]
    GeneIDi4830.
    KEGGihsa:4830.
    UCSCiuc002ith.2. human. [P15531-2]
    uc002iti.2. human. [P15531-1]

    Polymorphism databases

    DMDMi127981.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17620 mRNA. Translation: CAA35621.1 . Different initiation.
    X73066 mRNA. Translation: CAA51527.1 .
    X75598 Genomic DNA. Translation: CAA53270.1 .
    AF487339 mRNA. Translation: AAO85436.1 .
    AK291105 mRNA. Translation: BAF83794.1 .
    CR542104 mRNA. Translation: CAG46901.1 .
    CR542115 mRNA. Translation: CAG46912.1 .
    AC005839 Genomic DNA. No translation available.
    CH471109 Genomic DNA. Translation: EAW94568.1 .
    BC000293 mRNA. Translation: AAH00293.1 .
    BC018994 mRNA. Translation: AAH18994.1 .
    CCDSi CCDS11578.1. [P15531-2 ]
    CCDS11579.1. [P15531-1 ]
    PIRi A33386.
    RefSeqi NP_000260.1. NM_000269.2. [P15531-1 ]
    NP_937818.1. NM_198175.1. [P15531-2 ]
    UniGenei Hs.463456.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1JXV X-ray 2.20 A/B/C/D/E/F 1-152 [» ]
    1UCN X-ray 2.00 A/B/C 1-152 [» ]
    2HVD X-ray 2.15 A/B/C 1-152 [» ]
    2HVE X-ray 2.40 A/B/C 1-152 [» ]
    3L7U X-ray 2.10 A/B/C 1-152 [» ]
    4ENO X-ray 2.80 A/B 1-152 [» ]
    ProteinModelPortali P15531.
    SMRi P15531. Positions 1-152.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 110894. 46 interactions.
    DIPi DIP-39164N.
    IntActi P15531. 22 interactions.
    MINTi MINT-221462.
    STRINGi 9606.ENSP00000013034.

    Chemistry

    ChEMBLi CHEMBL2159.
    DrugBanki DB00441. Gemcitabine.
    DB00396. Progesterone.

    PTM databases

    PhosphoSitei P15531.

    Polymorphism databases

    DMDMi 127981.

    2D gel databases

    DOSAC-COBS-2DPAGE P15531.
    OGPi P15531.

    Proteomic databases

    MaxQBi P15531.
    PaxDbi P15531.
    PRIDEi P15531.

    Protocols and materials databases

    DNASUi 4830.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000013034 ; ENSP00000013034 ; ENSG00000239672 . [P15531-2 ]
    ENST00000336097 ; ENSP00000337060 ; ENSG00000239672 . [P15531-2 ]
    ENST00000393196 ; ENSP00000376892 ; ENSG00000239672 . [P15531-1 ]
    GeneIDi 4830.
    KEGGi hsa:4830.
    UCSCi uc002ith.2. human. [P15531-2 ]
    uc002iti.2. human. [P15531-1 ]

    Organism-specific databases

    CTDi 4830.
    GeneCardsi GC17P049231.
    HGNCi HGNC:7849. NME1.
    HPAi HPA008467.
    HPA041113.
    MIMi 156490. gene.
    neXtProti NX_P15531.
    PharmGKBi PA249.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0105.
    HOGENOMi HOG000224564.
    HOVERGENi HBG000423.
    KOi K00940.
    OMAi CAHEWIY.
    OrthoDBi EOG7GJ6FG.
    PhylomeDBi P15531.
    TreeFami TF106373.

    Enzyme and pathway databases

    BioCyci MetaCyc:ENSG00000011052-MONOMER.
    Reactomei REACT_21330. Synthesis and interconversion of nucleotide di- and triphosphates.

    Miscellaneous databases

    EvolutionaryTracei P15531.
    GeneWikii NME1.
    GenomeRNAii 4830.
    NextBioi 18606.
    PROi P15531.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P15531.
    Bgeei P15531.
    CleanExi HS_NME1.
    Genevestigatori P15531.

    Family and domain databases

    Gene3Di 3.30.70.141. 1 hit.
    HAMAPi MF_00451. NDP_kinase.
    InterProi IPR001564. Nucleoside_diP_kinase.
    IPR023005. Nucleoside_diP_kinase_AS.
    [Graphical view ]
    Pfami PF00334. NDK. 1 hit.
    [Graphical view ]
    PRINTSi PR01243. NUCDPKINASE.
    SMARTi SM00562. NDK. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54919. SSF54919. 1 hit.
    PROSITEi PS00469. NDP_KINASES. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Reduced Nm23/Awd protein in tumour metastasis and aberrant Drosophila development."
      Rosengard A.M., Krutzsch H.C., Shearn A., Biggs J.R., Barker E., Margulies I.M.K., King C.R., Liotta L.A., Steeg P.S.
      Nature 342:177-180(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "Nucleoside diphosphate kinase from human erythrocytes. Structural characterization of the two polypeptide chains responsible for heterogeneity of the hexameric enzyme."
      Gilles A.-M., Presecan E., Vonica A., Lascu I.
      J. Biol. Chem. 266:8784-8789(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE (ISOFORM 1), SUBUNIT, ACTIVE SITE.
    3. "Mutation in the nm23 gene is associated with metastasis in colorectal cancer."
      Wang L., Patel U., Ghosh L., Chen H.C., Banerjee S.
      Cancer Res. 53:717-720(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    4. "Isolation and characterization of the human genomic locus coding for the putative metastasis control gene nm23-H1."
      Dooley S., Seib T., Engel M., Theisinger B., Janz H., Piontek K., Zang K.D., Welter C.
      Hum. Genet. 93:63-66(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
    5. "Isolation and characterization of a novel human NM23-H1B gene, a different transcript of NM23-H1."
      Ni X., Gu S., Dai J., Cheng H., Guo L., Li L., Ji C., Xie Y., Ying K., Mao Y.
      J. Hum. Genet. 48:96-100(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY.
    6. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    7. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    8. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
      Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
      , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
      Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    9. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    10. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain and Lung.
    11. "High levels of p19/nm23 protein in neuroblastoma are associated with advanced stage disease and with N-myc gene amplification."
      Hailat N., Keim D.R., Melhem R.F., Zhu X.X., Eckerskorn C., Brodeur G.M., Reynolds C.P., Seeger R.C., Lottspeich F., Strahler J.R., Hanash S.J.
      J. Clin. Invest. 88:341-345(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 7-18; 40-49 AND 89-94 (ISOFORMS 1/2), DISCUSSION OF THE ROLE IN TUMOR PROGRESSION.
      Tissue: Neuroblastoma.
    12. Lubec G., Afjehi-Sadat L., Chen W.-Q., Sun Y.
      Submitted (DEC-2008) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 7-26; 40-49; 57-85 AND 89-128 (ISOFORMS 1/2), IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Brain, Cajal-Retzius cell and Fetal brain cortex.
    13. "Site-directed mutagenesis of nm23-H1. Mutation of proline 96 or serine 120 abrogates its motility inhibitory activity upon transfection into human breast carcinoma cells."
      MacDonald N.J., Freije J.M., Stracke M.L., Manrow R.E., Steeg P.S.
      J. Biol. Chem. 271:25107-25116(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF PRO-96; HIS-118 AND SER-120.
    14. "Identification of genes (SPON2 and C20orf2) differentially expressed between cancerous and noncancerous lung cells by mRNA differential display."
      Manda R., Kohno T., Matsuno Y., Takenoshita S., Kuwano H., Yokota J.
      Genomics 61:5-14(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    15. "Tumor suppressor NM23-H1 is a granzyme A-activated DNase during CTL-mediated apoptosis, and the nucleosome assembly protein SET is its inhibitor."
      Fan Z., Beresford P.J., Oh D.Y., Zhang D., Lieberman J.
      Cell 112:659-672(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ENZYME REGULATION, INTERACTION WITH SET, IDENTIFICATION IN THE SET COMPLEX.
    16. "Read-through transcript from NM23-H1 into the neighboring NM23-H2 gene encodes a novel protein, NM23-LV."
      Valentijn L.J., Koster J., Versteeg R.
      Genomics 87:483-489(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: ALTERNATIVE SPLICING (ISOFORM 3), SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    17. "The exonuclease TREX1 is in the SET complex and acts in concert with NM23-H1 to degrade DNA during granzyme A-mediated cell death."
      Chowdhury D., Beresford P.J., Zhu P., Zhang D., Sung J.S., Demple B., Perrino F.W., Lieberman J.
      Mol. Cell 23:133-142(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH TREX1, IDENTIFICATION IN THE SET COMPLEX.
    18. "Phosphorylation of nm23-H1 by CKI induces its complex formation with h-prune and promotes cell motility."
      Garzia L., D'Angelo A., Amoresano A., Knauer S.K., Cirulli C., Campanella C., Stauber R.H., Steegborn C., Iolascon A., Zollo M.
      Oncogene 27:1853-1864(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PRUNE.
    19. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    20. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    21. "Crystal structure of human nucleoside diphosphate kinase A, a metastasis suppressor."
      Min K., Song H.K., Chang C., Kim S.Y., Lee K.J., Suh S.W.
      Proteins 46:340-342(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
    22. "Nucleotide binding to nucleoside diphosphate kinases: X-ray structure of human NDPK-A in complex with ADP and comparison to protein kinases."
      Chen Y., Gallois-Montbrun S., Schneider B., Veron M., Morera S., Deville-Bonne D., Janin J.
      J. Mol. Biol. 332:915-926(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), MUTAGENESIS OF PHE-60 AND HIS-118.
    23. Cited for: VARIANT GLY-120.

    Entry informationi

    Entry nameiNDKA_HUMAN
    AccessioniPrimary (citable) accession number: P15531
    Secondary accession number(s): Q6FGK3, Q86XQ2, Q9UDJ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: April 1, 1990
    Last modified: October 1, 2014
    This is version 172 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    The role of this protein in tumor development and progression is uncertain. This protein is found in reduced amount in some tumor cells of high metastatic potential. However, increased NME1 levels correlate with aggressive tumor features in neuroblastoma. May have distinct if not opposite roles in different tumors.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3