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P15515 (HIS1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Histatin-1
Alternative name(s):
Histidine-rich protein 1
Post-PB protein
Short name=PPB

Cleaved into the following chain:

  1. His1-(31-57)-peptide
    Short name=His1 31/57
    Alternative name(s):
    His1-(12-38)-peptide
    Short name=His1 12/38
    Histatin-2
Gene names
Name:HTN1
Synonyms:HIS1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length57 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Histatins are salivary proteins that are considered to be major precursors of the protective proteinaceous structure on tooth surfaces (enamel pellicle). In addition, histatins exhibit antibacterial and antifungal activities.

Subcellular location

Secreted.

Tissue specificity

Submandibular and parotid glands. Ref.8

Post-translational modification

Depending on the authors, the form called histatin-2 is alternatively a proteolytic product, or the non-phosphorylated form of histatin-1. Ref.4 Ref.8

Miscellaneous

The recommended nomenclature of salivary peptides follows published guidelines (Ref.9). In agreement with the authors, it has been decided to indicate the boundaries of the peptides according to the positions within the precursor, and not in the mature protein, as has formerly been proposed.

Sequence similarities

Belongs to the histatin/statherin family.

Mass spectrometry

Molecular mass is 4848.2±0.5 Da from positions 20 - 50. Determined by ESI. Not post-translationally modified. Ref.8

Molecular mass is 4928.2±0.5 Da from positions 20 - 50. Determined by ESI. with 1 phosphate group. Ref.8

Molecular mass is 5008.6±0.5 Da from positions 20 - 50. Determined by ESI. with 1 phosphate group and 1 sulfate group. Ref.8

Molecular mass is 5088.4±0.5 Da from positions 20 - 50. Determined by ESI. with 1 phosphate group and 2 sulfate groups. Ref.8

Molecular mass is 5168.2±0.5 Da from positions 20 - 50. Determined by ESI. with 1 phosphate group and 3 sulfate groups. Ref.8

Molecular mass is 5247.7±0.5 Da from positions 20 - 50. Determined by ESI. with 1 phosphate group and 4 sulfate groups. Ref.8

Binary interactions

With

Entry

#Exp.

IntAct

Notes

MUC7Q8TAX72EBI-738638,EBI-738582

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Ref.4 Ref.5 Ref.6
Peptide20 – 5738Histatin-1 Ref.4 Ref.5 Ref.6
PRO_0000021416
Peptide31 – 5727His1-(31-57)-peptide
PRO_0000021417

Amino acid modifications

Modified residue211Phosphoserine Ref.4 Ref.5
Modified residue461Sulfotyrosine; in submandibular gland form Ref.8
Modified residue491Sulfotyrosine; in submandibular gland form Ref.8
Modified residue531Sulfotyrosine; in submandibular gland form Ref.8
Modified residue551Sulfotyrosine; in submandibular gland form Ref.8

Sequences

Sequence LengthMass (Da)Tools
P15515 [UniParc].

Last modified February 1, 1991. Version 2.
Checksum: F3532BD1DCE23D83

FASTA576,963
        10         20         30         40         50 
MKFFVFALVL ALMISMISAD SHEKRHHGYR RKFHEKHHSH REFPFYGDYG SNYLYDN 

« Hide

References

« Hide 'large scale' references
[1]"Histatins, a family of salivary histidine-rich proteins, are encoded by at least two loci (HIS1 and HIS2)."
Sabatini L.M., Azen E.A.
Biochem. Biophys. Res. Commun. 160:495-502(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Nucleotide sequence analysis of the human salivary protein genes HIS1 and HIS2, and evolution of the STATH/HIS gene family."
Chen Z.W.
Mol. Biol. Evol. 10:497-511(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Skeletal muscle.
[4]"Histatins, a novel family of histidine-rich proteins in human parotid secretion. Isolation, characterization, primary structure, and fungistatic effects on Candida albicans."
Oppenheim F.G., Xu T., McMillian F.M., Levitz S.M., Diamond R.D., Offner G.D., Troxler R.F.
J. Biol. Chem. 263:7472-7477(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 20-57, PHOSPHORYLATION AT SER-21.
Tissue: Parotid gland.
[5]"The primary structure and functional characterization of the neutral histidine-rich polypeptide from human parotid secretion."
Oppenheim F.G., Yang Y.C., Diamond R.D., Hyslop D., Offner G.D., Troxler R.F.
J. Biol. Chem. 261:1177-1182(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 20-57.
Tissue: Parotid gland.
[6]"Rapid purification and characterization of histatins (histidine-rich polypeptides) from human whole saliva."
Sugiyama K., Ogino T., Ogata K.
Arch. Oral Biol. 35:415-419(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 20-57.
Tissue: Saliva.
[7]"Localization of the genes for histatins to human chromosome 4q13 and tissue distribution of the mRNAs."
Vanderspek J.C., Wyandt H.E., Skare J.C., Milunsky A., Oppenheim F.G., Troxler R.F.
Am. J. Hum. Genet. 45:381-387(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 14-57.
[8]"Tyrosine polysulfation of human salivary histatin 1. A post-translational modification specific of the submandibular gland."
Cabras T., Fanali C., Monteiro J.A., Amado F., Inzitari R., Desiderio C., Scarano E., Giardina B., Castagnola M., Messana I.
J. Proteome Res. 6:2472-2480(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION, SULFATION AT TYR-46; TYR-49; TYR-53 AND TYR-55, TISSUE SPECIFICITY, MASS SPECTROMETRY.
[9]"Salivary peptidomics."
Amado F., Lobo M.J., Domingues P., Duarte J.A., Vitorino R.
Expert Rev. Proteomics 7:709-721(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PEPTIDE NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M26664 mRNA. Translation: AAA58645.1.
L04132 Genomic DNA. Translation: AAA02745.1.
BC017835 mRNA. Translation: AAH17835.1.
CCDSCCDS3534.1.
PIRA32541. I57425.
RefSeqNP_002150.1. NM_002159.2.
UniGeneHs.250959.

3D structure databases

ProteinModelPortalP15515.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109578. 1 interaction.
IntActP15515. 1 interaction.
STRING9606.ENSP00000246896.

PTM databases

PhosphoSiteP15515.

Proteomic databases

PaxDbP15515.
PeptideAtlasP15515.
PRIDEP15515.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000246896; ENSP00000246896; ENSG00000126550.
ENST00000511674; ENSP00000424501; ENSG00000126550.
GeneID3346.
KEGGhsa:3346.
UCSCuc003hex.3. human.

Organism-specific databases

CTD3346.
GeneCardsGC04P070916.
HGNCHGNC:5283. HTN1.
MIM142701. gene.
neXtProtNX_P15515.
PharmGKBPA29546.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG249966.
HOGENOMHOG000112905.
HOVERGENHBG005970.
InParanoidP15515.
KOK13913.
OMADYGSNYL.
OrthoDBEOG7TTQBV.
PhylomeDBP15515.
TreeFamTF341637.

Gene expression databases

BgeeP15515.
CleanExHS_HTN1.
GenevestigatorP15515.

Family and domain databases

ProtoNetSearch...

Other

ChiTaRSHTN1. human.
GeneWikiHTN1.
GenomeRNAi3346.
NextBio13236.
PROP15515.
SOURCESearch...

Entry information

Entry nameHIS1_HUMAN
AccessionPrimary (citable) accession number: P15515
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: February 1, 1991
Last modified: July 9, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM