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Protein

Spectrin beta chain, erythrocytic

Gene

Sptb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.

Miscellaneous

This complex is anchored to the cytoplasmic face of the plasma membrane via another protein, ankyrin, which binds to beta-spectrin and mediates the binding of the whole complex to a transmembrane protein band 3. The interaction of erythrocyte spectrin with other proteins through specific binding domains lead to the formation of an extensive subplasmalemmal meshwork which is thought to be responsible for the maintenance of the biconcave shape of human erythrocytes, for the regulation of plasma membrane components and for the maintenance of the lipid asymmetry of the plasma membrane.

GO - Molecular functioni

  • actin binding Source: MGI
  • actin filament binding Source: MGI
  • ankyrin binding Source: MGI
  • protein heterodimerization activity Source: MGI
  • structural constituent of cytoskeleton Source: InterPro

GO - Biological processi

  • actin filament capping Source: UniProtKB-KW
  • hemopoiesis Source: MGI
  • plasma membrane organization Source: MGI
  • porphyrin-containing compound biosynthetic process Source: MGI

Keywordsi

Molecular functionActin capping, Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Spectrin beta chain, erythrocytic
Alternative name(s):
Beta-I spectrin
Gene namesi
Name:Sptb
Synonyms:Spnb-1, Spnb1, Sptb1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:98387. Sptb.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000734601 – 2128Spectrin beta chain, erythrocyticAdd BLAST2128

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei36PhosphoserineCombined sources1
Modified residuei104PhosphothreonineBy similarity1
Modified residuei1289PhosphoserineCombined sources1
Modified residuei2034PhosphoserineBy similarity1
Modified residuei2064PhosphothreonineBy similarity1
Modified residuei2072PhosphothreonineCombined sources1
Modified residuei2101PhosphothreonineBy similarity1
Modified residuei2105PhosphoserineBy similarity1
Modified residuei2108PhosphoserineBy similarity1
Modified residuei2114PhosphoserineBy similarity1
Modified residuei2116PhosphoserineBy similarity1
Modified residuei2119PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP15508.
PaxDbiP15508.
PeptideAtlasiP15508.
PRIDEiP15508.

PTM databases

iPTMnetiP15508.
PhosphoSitePlusiP15508.

Expressioni

Gene expression databases

CleanExiMM_SPNB1.

Interactioni

Subunit structurei

Composed of nonhomologous chains, alpha and beta, which aggregate to form dimers, tetramers, and higher polymers.

GO - Molecular functioni

  • actin binding Source: MGI
  • actin filament binding Source: MGI
  • ankyrin binding Source: MGI
  • protein heterodimerization activity Source: MGI

Protein-protein interaction databases

IntActiP15508. 3 interactors.
MINTiMINT-4996478.
STRINGi10090.ENSMUSP00000021458.

Structurei

3D structure databases

ProteinModelPortaliP15508.
SMRiP15508.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini54 – 158Calponin-homology (CH) 1PROSITE-ProRule annotationAdd BLAST105
Domaini173 – 278Calponin-homology (CH) 2PROSITE-ProRule annotationAdd BLAST106
Repeati303 – 411Spectrin 1Sequence analysisAdd BLAST109
Repeati416 – 517Spectrin 2Sequence analysisAdd BLAST102
Repeati521 – 627Spectrin 3Sequence analysisAdd BLAST107
Repeati630 – 733Spectrin 4Sequence analysisAdd BLAST104
Repeati736 – 838Spectrin 5Sequence analysisAdd BLAST103
Repeati845 – 942Spectrin 6Sequence analysisAdd BLAST98
Repeati950 – 1050Spectrin 7Sequence analysisAdd BLAST101
Repeati1054 – 1157Spectrin 8Sequence analysisAdd BLAST104
Repeati1162 – 1250Spectrin 9Sequence analysisAdd BLAST89
Repeati1267 – 1368Spectrin 10Sequence analysisAdd BLAST102
Repeati1381 – 1455Spectrin 11Sequence analysisAdd BLAST75
Repeati1473 – 1574Spectrin 12Sequence analysisAdd BLAST102
Repeati1576 – 1680Spectrin 13Sequence analysisAdd BLAST105
Repeati1682 – 1784Spectrin 14Sequence analysisAdd BLAST103
Repeati1789 – 1890Spectrin 15Sequence analysisAdd BLAST102
Repeati1897 – 1997Spectrin 16Sequence analysisAdd BLAST101
Repeati2004 – 2064Spectrin 17Sequence analysisAdd BLAST61

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 275Actin-bindingAdd BLAST274

Sequence similaritiesi

Belongs to the spectrin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0517. Eukaryota.
COG5069. LUCA.
HOVERGENiHBG057912.
InParanoidiP15508.

Family and domain databases

CDDicd00014. CH. 2 hits.
Gene3Di1.10.418.10. 2 hits.
InterProiView protein in InterPro
IPR001589. Actinin_actin-bd_CS.
IPR036872. Calponin-like_dom_sf.
IPR001715. CH-domain.
IPR018159. Spectrin/alpha-actinin.
IPR016343. Spectrin_bsu.
IPR002017. Spectrin_repeat.
PfamiView protein in Pfam
PF00307. CH. 2 hits.
PF00435. Spectrin. 17 hits.
PIRSFiPIRSF002297. Spectrin_beta_subunit. 1 hit.
SMARTiView protein in SMART
SM00033. CH. 2 hits.
SM00150. SPEC. 17 hits.
SUPFAMiSSF47576. SSF47576. 1 hit.
PROSITEiView protein in PROSITE
PS00019. ACTININ_1. 1 hit.
PS00020. ACTININ_2. 1 hit.
PS50021. CH. 2 hits.

Sequencei

Sequence statusi: Complete.

P15508-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSATEFENV GNQPPFSRIN ARWDAPDDEL DNDNSSARLF ERSRIKALAD
60 70 80 90 100
EREVVQKKTF TKWVNSHLAR VSCRISDLYK DLRDGRMLIK LLEVLSGEML
110 120 130 140 150
PRPTKGKMRI HCLENVDKAL QFLKEQRVHL ENMGSHDIVD GNHRLVLGLI
160 170 180 190 200
WTIILRFQIQ DIVVQTQEGR EQRSAKDALL LWCQMKTAGY PHVNVTNFTS
210 220 230 240 250
SWKDGLAFNA LIHKHRPDLI DFDKLKDSNA RHNLEHAFDV AERQLGIIPL
260 270 280 290 300
LDPEDVFTEN PDEKSIITYV VAFYHYFSKM KVLAVEGKRV GKVIDHAIET
310 320 330 340 350
EKMIEKYSGL ASDLLTWIEQ TISVLNSRKF ANSLSGVQQQ LQAFSTYRTV
360 370 380 390 400
EKPPKFQEKG NLEVLLFTIQ SRMRANNQKV YTPHDGKLVS DINRAWESLE
410 420 430 440 450
EAEYQRELAL RSELIRQEFD RKAAMRETWL NENQRLVTQD NFGYDLAAVE
460 470 480 490 500
AAKKKHEAIE TDTAAYEERV KALEDLAQEL EKENYHDQKR IIARKDNILR
510 520 530 540 550
LWSYLQELLR SRRQRLEATL ALQKLFQDML HSIDWMDEIK AHILSAEFGK
560 570 580 590 600
HLLEVEDLLQ KHKLMEADIA IQGDKVKAIT AATLQFAEGK GYQPCDPQVI
610 620 630 640 650
QDRVSHLEQC FSELSNMAAG RKAQLEQSKR LWKFFWEMDE AESWIKEKEQ
660 670 680 690 700
IYSSLDYGKD LTSVLILQRK HKAFEDELRG LDAHLKQIFQ EADDMVAQKQ
710 720 730 740 750
FGHPQIETRV KEVSAQWDHL KELAAFRKKD LQDAENFFQF QGDADDLKAW
760 770 780 790 800
LQDAHRLLSG EDVGQDEGAT RALGKKHKEF LEELEESRGV MEHLEHQAQG
810 820 830 840 850
FPEEFRDSPD VTNRLQALRK LYQQVLTQAE LRGHKLQEAL DLYTVFGESD
860 870 880 890 900
ACELWMTEKG KWLDQMDIPN TLEDLEVVQH RFDILDQEMK TLMAQIDGVN
910 920 930 940 950
LAANNLVESG HPRSGEVKQY QDRLNKRWQA FQAVVSEQRE AVDSALRVNN
960 970 980 990 1000
YCVDCEETSK WIMDKTKVVE STKDLGQDLA GVIAIQRKLS GLERDVLAIR
1010 1020 1030 1040 1050
DRVSALERES QYLMESHPEQ KEDIGQRQAD VEKLWKGLQD ALQGQELSLG
1060 1070 1080 1090 1100
EASKLQAFLQ DLDDFKAWLS MAQKAVASED MPESLPEAEQ LLQQHAAIKE
1110 1120 1130 1140 1150
EIDAHRDDYH RVKASGEKVI EGQTDPDYQL LGQRLEGLDT DWDALRRMWE
1160 1170 1180 1190 1200
SRGNTLTQCL GFQEFQKDAK QAEAILSNQE YTLAHLEPPD SLAAAEAGIR
1210 1220 1230 1240 1250
KFEDFLVSME NNRDKILSPV DSGNKLVAEG NLYSNKIMEK VQLIEDRHKK
1260 1270 1280 1290 1300
NNEKAQEATV LLKDNLELQN FLQNCKELTL WINDKLLTSP DVSYDEARNL
1310 1320 1330 1340 1350
HNKWMKHQAF MAELASHQGW LENIDAEGRQ LMAEKPQFKD VVSERLEALH
1360 1370 1380 1390 1400
KLWEELQSTA KAKAEQLSAA RSSDLRLQTH ADLNKWIGAM EDQLRSDDLG
1410 1420 1430 1440 1450
KDLTTVNRML AKLKRVEEQV NLRKEELEEL FADAPSLGAE AGDTDMSIEK
1460 1470 1480 1490 1500
RFLDLLEPLG RRKKQLELSK AKLQISRDLE DETLWVEERL PLAQSADYGT
1510 1520 1530 1540 1550
NLQTVQLFMK KNQTLQNEIL GHAPRVEDVL RRGQELVKAA EIDCQDIEER
1560 1570 1580 1590 1600
LGHLQSSWDT LREAAAGRLQ RLRDAHEAQQ YYLDAGEAEA WISEQELYVF
1610 1620 1630 1640 1650
SDEPPKDEEG AIVMLKRHLR QQRTVEEYGR NIKQLAGRAQ SLLSAGHPEG
1660 1670 1680 1690 1700
EQIIRLQGQV DKQYAGLKDM AEERRRRLEN MYHLFQLKRE ADDLEQWITE
1710 1720 1730 1740 1750
KEMVASSQEM GQDFDHVTML RDKFRDFARE TGAIGQERVD NVTIIERLID
1760 1770 1780 1790 1800
AGHSEAATIA EWKDGLNDMW ADLLELIDTR MQLLAASYDL HRYFYTGTEI
1810 1820 1830 1840 1850
LGLIDEKHRE LPEDVGLDAS TAESFHRVHT AFERELHLLG VQVQQFQDVA
1860 1870 1880 1890 1900
TRLQTAYAGE KADAIQSKEQ EVSAAWQALL DACAGRRAQL VDTADKFRFF
1910 1920 1930 1940 1950
SMVRDLLSWM ESIIRQIETQ ERPRDVSSVE LLLKYHQGIK AEINTRAKNF
1960 1970 1980 1990 2000
STCLELGESL LQRQHQASDE IREKLQQVIS RRQEMNDKWE ARSDRLHMLL
2010 2020 2030 2040 2050
EVCQFSRDAS VAEAWLIAQE PYLASRDFGH TVDSVEKLIK RHEAFEKSTA
2060 2070 2080 2090 2100
SWAERFAALE KPTTLELKER QTPERPTEEP GPQEEEGETA GEAPQVHHAA
2110 2120
TERTSPVSFM SRLSSSWESL LPEPAHPF
Length:2,128
Mass (Da):245,250
Last modified:January 23, 2007 - v4
Checksum:i10E576111106DFE1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S66283 mRNA. Translation: AAB28600.1.
M18641 mRNA. Translation: AAA40126.1.
PIRiA45929.
UniGeneiMm.32881.

Similar proteinsi

Entry informationi

Entry nameiSPTB1_MOUSE
AccessioniPrimary (citable) accession number: P15508
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 23, 2007
Last modified: October 25, 2017
This is version 139 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families