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Protein

Glycine dehydrogenase (decarboxylating), mitochondrial

Gene

GLDC

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The glycine cleavage system catalyzes the degradation of glycine. The P protein (GLDC) binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein (GCSH).3 Publications

Catalytic activityi

Glycine + [glycine-cleavage complex H protein]-N6-lipoyl-L-lysine = [glycine-cleavage complex H protein]-S-aminomethyl-N6-dihydrolipoyl-L-lysine + CO2.3 Publications

Cofactori

pyridoxal 5'-phosphate1 Publication

Enzyme regulationi

Stimulated by lipoic acid. Inhibited in presence of methylamine.2 Publications

Kineticsi

  1. KM=20 mM for glycine in presence of GCSH1 Publication
  2. KM=27 mM for methylamine in presence of GCSH1 Publication
  3. KM=40 mM for glycine1 Publication
  4. KM=3.5 mM for lipoic acid1 Publication
  5. KM=63 mM for methylamine1 Publication

    GO - Molecular functioni

    • glycine dehydrogenase (decarboxylating) activity Source: UniProtKB
    • glycine dehydrogenase activity Source: UniProtKB
    • lyase activity Source: InterPro
    • protein homodimerization activity Source: UniProtKB
    • pyridoxal binding Source: UniProtKB

    GO - Biological processi

    • glycine catabolic process Source: UniProtKB
    • response to lipoic acid Source: UniProtKB
    • response to methylamine Source: UniProtKB

    Keywordsi

    Molecular functionOxidoreductase
    LigandPyridoxal phosphate

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-12926
    BRENDAi1.4.4.2 1306

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycine dehydrogenase (decarboxylating), mitochondrialCurated (EC:1.4.4.23 Publications)
    Alternative name(s):
    Glycine cleavage system P protein
    Glycine decarboxylase1 Publication
    Glycine dehydrogenase (aminomethyl-transferring)
    Gene namesi
    Name:GLDCBy similarity
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
    Proteomesi
    • UP000000539 Componenti: Unplaced

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_0000010742? – 1004Glycine dehydrogenase (decarboxylating), mitochondrial
    Transit peptidei1 – ?MitochondrionSequence analysis

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei738N6-(pyridoxal phosphate)lysine1 Publication1

    Proteomic databases

    PaxDbiP15505
    PRIDEiP15505

    Expressioni

    Tissue specificityi

    Liver (at protein level).1 Publication

    Interactioni

    Subunit structurei

    Homodimer. Interacts with GCSH. The glycine cleavage system is composed of four proteins: P (GLDC), T (GCST), L (DLD) and H (GCSH).2 Publications

    GO - Molecular functioni

    • protein homodimerization activity Source: UniProtKB

    Protein-protein interaction databases

    STRINGi9031.ENSGALP00000037556

    Structurei

    3D structure databases

    ProteinModelPortaliP15505
    SMRiP15505
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GcvP family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiKOG2040 Eukaryota
    COG0403 LUCA
    COG1003 LUCA
    HOGENOMiHOG000239369
    HOVERGENiHBG005820
    InParanoidiP15505
    KOiK00281
    PhylomeDBiP15505

    Family and domain databases

    CDDicd00613 GDC-P, 2 hits
    Gene3Di3.40.640.10, 2 hits
    3.90.1150.10, 1 hit
    HAMAPiMF_00711 GcvP, 1 hit
    InterProiView protein in InterPro
    IPR001597 ArAA_b-elim_lyase/Thr_aldolase
    IPR003437 GcvP
    IPR020581 GDC_P
    IPR015424 PyrdxlP-dep_Trfase
    IPR015422 PyrdxlP-dep_Trfase_dom1
    IPR015421 PyrdxlP-dep_Trfase_major
    PANTHERiPTHR11773 PTHR11773, 1 hit
    PfamiView protein in Pfam
    PF01212 Beta_elim_lyase, 1 hit
    PF02347 GDC-P, 1 hit
    SUPFAMiSSF53383 SSF53383, 3 hits
    TIGRFAMsiTIGR00461 gcvP, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P15505-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MQSCGRWWGR LAARGAPRHL RPAAGGPRRQ QQRWGGGEAA RCIEQLLPRH
    60 70 80 90 100
    DDFCRRHIGP REREKREMLS AVGVQSVEEL MDKTIPASIR LRRPLRMDDH
    110 120 130 140 150
    VVENEILETL YNIASKNKIW RSYIGMGYYN CSVPQPIARN LLENAGWVTQ
    160 170 180 190 200
    YTPYQPEVSQ GRLESLLNYQ TMVCDITGMD VANASLLDEG TAAAEAMQLC
    210 220 230 240 250
    HRQNKRRKFY IDARCHPQTI ANYTGVITEL KLPHEMDFSG KDVSGVLFQY
    260 270 280 290 300
    PDTEGKVEDF SELIERAHQN GTLACCATDL LALCILKPPG EFGVDVVLGS
    310 320 330 340 350
    SQRFGVPLCY GGPHAAFFAV KENLVRMMPG RMVGVTRDAN GKEVYRLALQ
    360 370 380 390 400
    TREQHIRRDK ATSNICTAQA LLANMAAMYG VYHGSDGLKD IARRVHNATL
    410 420 430 440 450
    ILAEGLRRAG HKLHHDLFFD TLTVTCGCSV KEVLDRAALR KINVRIYSDG
    460 470 480 490 500
    RLGVSLDETV NEKDLDDILW IFGCESSAEL VAEGMGEETK GILSTPFKRT
    510 520 530 540 550
    SKFLTHQVFN SYHSETNIVR YMKRLENKDI SLVHSMIPLG SCTMKLNSSA
    560 570 580 590 600
    ELAPISWKEF ANIHPFVPLD QAQGYQQLFK DLEKDLCEIT GYDKISFQPN
    610 620 630 640 650
    SGAQGEYAGL AAIKAYLNAK GERHRSVCLI PRSAHGTNPA SAQMAGMKIQ
    660 670 680 690 700
    PIEVDKNGSI DISHLKAMVD KHKENLAAIM ITYPSTNGVF EEEIGDVCDL
    710 720 730 740 750
    IHKHGGQVYL DGANMNAQVG LCRPGDYGSD VSHLNLHKTF CIPHGGGGPG
    760 770 780 790 800
    MGPIGVKKHL APYLPTHPVI KIQTDKDACP LGTVSAAPWG SSAILPISWV
    810 820 830 840 850
    YIKTMGAKGL KHASEIAILN ANYMAKRLEK HYKILFRGVR GYVAHEFILD
    860 870 880 890 900
    TRPFKKTANI EAVDLAKRLQ DYGFHAPTMS WPVAGTLMIE PTESEDKGEL
    910 920 930 940 950
    DRFCDAMISI RQEIADIEEG RMDPQVNPLK MSPHTLNCVT SSKWDRPYSR
    960 970 980 990 1000
    EVAAFPLPFV KPESKFWPTI ARIDDIYGDQ HLVCTCPPME AYESPFSEQK

    RASS
    Length:1,004
    Mass (Da):111,852
    Last modified:May 1, 1992 - v2
    Checksum:i4446D7C66E0DC4BD
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M64402 mRNA Translation: AAA49029.1
    D90266 mRNA Translation: BAA14313.1
    D90240 Genomic DNA Translation: BAA14287.1
    PIRiA39521
    RefSeqiNP_989653.1, NM_204322.1
    UniGeneiGga.3393

    Genome annotation databases

    GeneIDi374222
    KEGGigga:374222

    Similar proteinsi

    Entry informationi

    Entry nameiGCSP_CHICK
    AccessioniPrimary (citable) accession number: P15505
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: May 1, 1992
    Last modified: March 28, 2018
    This is version 114 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health