Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P15505

- GCSP_CHICK

UniProt

P15505 - GCSP_CHICK

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Glycine dehydrogenase (decarboxylating), mitochondrial

Gene

GLDC

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

The glycine cleavage system catalyzes the degradation of glycine. The P protein (GLDC) binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein (GCSH).2 Publications

Catalytic activityi

Glycine + [glycine-cleavage complex H protein]-N(6)-lipoyl-L-lysine = [glycine-cleavage complex H protein]-S-aminomethyl-N(6)-dihydrolipoyl-L-lysine + CO2.2 Publications

Cofactori

pyridoxal 5'-phosphate1 Publication

Enzyme regulationi

Stimulated by lipoic acid. Inhibited in presence of methylamine.2 Publications

Kineticsi

  1. KM=20 mM for glycine in presence of GCSH (in PubMed:7440563)2 Publications
  2. KM=27 mM for methylamine in presence of GCSH (in PubMed:7440563)2 Publications
  3. KM=40 mM for glycine (in PubMed:7440562)2 Publications
  4. KM=3.5 mM for lipoic acid (in PubMed:7440562)2 Publications
  5. KM=63 mM for methylamine (in PubMed:7440562)2 Publications

GO - Molecular functioni

  1. glycine dehydrogenase (decarboxylating) activity Source: UniProtKB-EC
  2. lyase activity Source: InterPro
  3. pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  1. glycine catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12926.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine dehydrogenase (decarboxylating), mitochondrial (EC:1.4.4.2)
Alternative name(s):
Glycine cleavage system P protein
Glycine decarboxylase
Glycine dehydrogenase (aminomethyl-transferring)
Gene namesi
Name:GLDC
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Unplaced

Subcellular locationi

Mitochondrion 1 Publication

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 1004Glycine dehydrogenase (decarboxylating), mitochondrialPRO_0000010742
Transit peptidei1 – ?MitochondrionSequence Analysis

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei738 – 7381N6-(pyridoxal phosphate)lysine

Proteomic databases

PaxDbiP15505.

Expressioni

Tissue specificityi

Liver (at protein level).1 Publication

Interactioni

Subunit structurei

Homodimer. The glycine cleavage system is composed of four proteins: P (GLDC), T (GCST), L (DLD) and H (GCSH). Interacts with GCSH.2 Publications

Protein-protein interaction databases

STRINGi9031.ENSGALP00000037556.

Structurei

3D structure databases

ProteinModelPortaliP15505.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GcvP family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1003.
HOGENOMiHOG000239369.
HOVERGENiHBG005820.
InParanoidiP15505.
KOiK00281.
PhylomeDBiP15505.

Family and domain databases

Gene3Di3.40.640.10. 2 hits.
HAMAPiMF_00711. GcvP.
InterProiIPR001597. ArAA_b-elim_lyase/Thr_aldolase.
IPR020580. GDC-P_N.
IPR020581. GDC_P.
IPR003437. GDC_P_homo.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERiPTHR11773. PTHR11773. 1 hit.
PfamiPF01212. Beta_elim_lyase. 1 hit.
PF02347. GDC-P. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 3 hits.
TIGRFAMsiTIGR00461. gcvP. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15505-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQSCGRWWGR LAARGAPRHL RPAAGGPRRQ QQRWGGGEAA RCIEQLLPRH
60 70 80 90 100
DDFCRRHIGP REREKREMLS AVGVQSVEEL MDKTIPASIR LRRPLRMDDH
110 120 130 140 150
VVENEILETL YNIASKNKIW RSYIGMGYYN CSVPQPIARN LLENAGWVTQ
160 170 180 190 200
YTPYQPEVSQ GRLESLLNYQ TMVCDITGMD VANASLLDEG TAAAEAMQLC
210 220 230 240 250
HRQNKRRKFY IDARCHPQTI ANYTGVITEL KLPHEMDFSG KDVSGVLFQY
260 270 280 290 300
PDTEGKVEDF SELIERAHQN GTLACCATDL LALCILKPPG EFGVDVVLGS
310 320 330 340 350
SQRFGVPLCY GGPHAAFFAV KENLVRMMPG RMVGVTRDAN GKEVYRLALQ
360 370 380 390 400
TREQHIRRDK ATSNICTAQA LLANMAAMYG VYHGSDGLKD IARRVHNATL
410 420 430 440 450
ILAEGLRRAG HKLHHDLFFD TLTVTCGCSV KEVLDRAALR KINVRIYSDG
460 470 480 490 500
RLGVSLDETV NEKDLDDILW IFGCESSAEL VAEGMGEETK GILSTPFKRT
510 520 530 540 550
SKFLTHQVFN SYHSETNIVR YMKRLENKDI SLVHSMIPLG SCTMKLNSSA
560 570 580 590 600
ELAPISWKEF ANIHPFVPLD QAQGYQQLFK DLEKDLCEIT GYDKISFQPN
610 620 630 640 650
SGAQGEYAGL AAIKAYLNAK GERHRSVCLI PRSAHGTNPA SAQMAGMKIQ
660 670 680 690 700
PIEVDKNGSI DISHLKAMVD KHKENLAAIM ITYPSTNGVF EEEIGDVCDL
710 720 730 740 750
IHKHGGQVYL DGANMNAQVG LCRPGDYGSD VSHLNLHKTF CIPHGGGGPG
760 770 780 790 800
MGPIGVKKHL APYLPTHPVI KIQTDKDACP LGTVSAAPWG SSAILPISWV
810 820 830 840 850
YIKTMGAKGL KHASEIAILN ANYMAKRLEK HYKILFRGVR GYVAHEFILD
860 870 880 890 900
TRPFKKTANI EAVDLAKRLQ DYGFHAPTMS WPVAGTLMIE PTESEDKGEL
910 920 930 940 950
DRFCDAMISI RQEIADIEEG RMDPQVNPLK MSPHTLNCVT SSKWDRPYSR
960 970 980 990 1000
EVAAFPLPFV KPESKFWPTI ARIDDIYGDQ HLVCTCPPME AYESPFSEQK

RASS
Length:1,004
Mass (Da):111,852
Last modified:May 1, 1992 - v2
Checksum:i4446D7C66E0DC4BD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64402 mRNA. Translation: AAA49029.1.
D90266 mRNA. Translation: BAA14313.1.
D90240 Genomic DNA. Translation: BAA14287.1.
PIRiA39521.
RefSeqiNP_989653.1. NM_204322.1.
UniGeneiGga.3393.

Genome annotation databases

GeneIDi374222.
KEGGigga:374222.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64402 mRNA. Translation: AAA49029.1 .
D90266 mRNA. Translation: BAA14313.1 .
D90240 Genomic DNA. Translation: BAA14287.1 .
PIRi A39521.
RefSeqi NP_989653.1. NM_204322.1.
UniGenei Gga.3393.

3D structure databases

ProteinModelPortali P15505.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9031.ENSGALP00000037556.

Proteomic databases

PaxDbi P15505.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 374222.
KEGGi gga:374222.

Organism-specific databases

CTDi 2731.

Phylogenomic databases

eggNOGi COG1003.
HOGENOMi HOG000239369.
HOVERGENi HBG005820.
InParanoidi P15505.
KOi K00281.
PhylomeDBi P15505.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-12926.

Miscellaneous databases

NextBioi 20813724.
PROi P15505.

Family and domain databases

Gene3Di 3.40.640.10. 2 hits.
HAMAPi MF_00711. GcvP.
InterProi IPR001597. ArAA_b-elim_lyase/Thr_aldolase.
IPR020580. GDC-P_N.
IPR020581. GDC_P.
IPR003437. GDC_P_homo.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view ]
PANTHERi PTHR11773. PTHR11773. 1 hit.
Pfami PF01212. Beta_elim_lyase. 1 hit.
PF02347. GDC-P. 1 hit.
[Graphical view ]
SUPFAMi SSF53383. SSF53383. 3 hits.
TIGRFAMsi TIGR00461. gcvP. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The glycine cleavage system. Molecular cloning of the chicken and human glycine decarboxylase cDNAs and some characteristics involved in the deduced protein structures."
    Kume A., Koyata H., Sakakibara T., Ishiguro Y., Kure S., Hiraga K.
    J. Biol. Chem. 266:3323-3329(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: White leghorn.
  2. "Amino acid sequence of the phosphopyridoxyl peptide from P-protein of the chicken liver glycine cleavage system."
    Fujiwara K., Okamura-Ikeda K., Motokawa Y.
    Biochem. Biophys. Res. Commun. 149:621-627(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 704-757.
    Tissue: Liver.
  3. "The mitochondrial glycine cleavage system. Purification and properties of glycine decarboxylase from chicken liver mitochondria."
    Hiraga K., Kikuchi G.
    J. Biol. Chem. 255:11664-11670(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, ENZYME REGULATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, FUNCTION.
  4. "The mitochondrial glycine cleavage system. Functional association of glycine decarboxylase and aminomethyl carrier protein."
    Hiraga K., Kikuchi G.
    J. Biol. Chem. 255:11671-11676(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, INTERACTION WITH GCSH, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, FUNCTION.

Entry informationi

Entry nameiGCSP_CHICK
AccessioniPrimary (citable) accession number: P15505
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: May 1, 1992
Last modified: November 26, 2014
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3