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Protein

Glycine dehydrogenase (decarboxylating), mitochondrial

Gene

GLDC

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The glycine cleavage system catalyzes the degradation of glycine. The P protein (GLDC) binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein (GCSH).2 Publications

Catalytic activityi

Glycine + [glycine-cleavage complex H protein]-N(6)-lipoyl-L-lysine = [glycine-cleavage complex H protein]-S-aminomethyl-N(6)-dihydrolipoyl-L-lysine + CO2.2 Publications

Cofactori

pyridoxal 5'-phosphate1 Publication

Enzyme regulationi

Stimulated by lipoic acid. Inhibited in presence of methylamine.2 Publications

Kineticsi

  1. KM=20 mM for glycine in presence of GCSH (in PubMed:7440563)2 Publications
  2. KM=27 mM for methylamine in presence of GCSH (in PubMed:7440563)2 Publications
  3. KM=40 mM for glycine (in PubMed:7440562)2 Publications
  4. KM=3.5 mM for lipoic acid (in PubMed:7440562)2 Publications
  5. KM=63 mM for methylamine (in PubMed:7440562)2 Publications

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-12926.
    BRENDAi1.4.4.2. 1306.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycine dehydrogenase (decarboxylating), mitochondrial (EC:1.4.4.2)
    Alternative name(s):
    Glycine cleavage system P protein
    Glycine decarboxylase
    Glycine dehydrogenase (aminomethyl-transferring)
    Gene namesi
    Name:GLDC
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539 Componenti: Unplaced

    Subcellular locationi

    • Mitochondrion 1 Publication

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 1004Glycine dehydrogenase (decarboxylating), mitochondrialPRO_0000010742
    Transit peptidei1 – ?MitochondrionSequence Analysis

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei738 – 7381N6-(pyridoxal phosphate)lysine

    Proteomic databases

    PaxDbiP15505.

    Expressioni

    Tissue specificityi

    Liver (at protein level).1 Publication

    Interactioni

    Subunit structurei

    Homodimer. The glycine cleavage system is composed of four proteins: P (GLDC), T (GCST), L (DLD) and H (GCSH). Interacts with GCSH.2 Publications

    Protein-protein interaction databases

    STRINGi9031.ENSGALP00000037556.

    Structurei

    3D structure databases

    ProteinModelPortaliP15505.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GcvP family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1003.
    HOGENOMiHOG000239369.
    HOVERGENiHBG005820.
    InParanoidiP15505.
    KOiK00281.
    PhylomeDBiP15505.

    Family and domain databases

    Gene3Di3.40.640.10. 2 hits.
    HAMAPiMF_00711. GcvP.
    InterProiIPR001597. ArAA_b-elim_lyase/Thr_aldolase.
    IPR003437. GcvP.
    IPR020580. GDC-P_N.
    IPR020581. GDC_P.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    [Graphical view]
    PANTHERiPTHR11773. PTHR11773. 1 hit.
    PfamiPF01212. Beta_elim_lyase. 1 hit.
    PF02347. GDC-P. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 3 hits.
    TIGRFAMsiTIGR00461. gcvP. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P15505-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MQSCGRWWGR LAARGAPRHL RPAAGGPRRQ QQRWGGGEAA RCIEQLLPRH
    60 70 80 90 100
    DDFCRRHIGP REREKREMLS AVGVQSVEEL MDKTIPASIR LRRPLRMDDH
    110 120 130 140 150
    VVENEILETL YNIASKNKIW RSYIGMGYYN CSVPQPIARN LLENAGWVTQ
    160 170 180 190 200
    YTPYQPEVSQ GRLESLLNYQ TMVCDITGMD VANASLLDEG TAAAEAMQLC
    210 220 230 240 250
    HRQNKRRKFY IDARCHPQTI ANYTGVITEL KLPHEMDFSG KDVSGVLFQY
    260 270 280 290 300
    PDTEGKVEDF SELIERAHQN GTLACCATDL LALCILKPPG EFGVDVVLGS
    310 320 330 340 350
    SQRFGVPLCY GGPHAAFFAV KENLVRMMPG RMVGVTRDAN GKEVYRLALQ
    360 370 380 390 400
    TREQHIRRDK ATSNICTAQA LLANMAAMYG VYHGSDGLKD IARRVHNATL
    410 420 430 440 450
    ILAEGLRRAG HKLHHDLFFD TLTVTCGCSV KEVLDRAALR KINVRIYSDG
    460 470 480 490 500
    RLGVSLDETV NEKDLDDILW IFGCESSAEL VAEGMGEETK GILSTPFKRT
    510 520 530 540 550
    SKFLTHQVFN SYHSETNIVR YMKRLENKDI SLVHSMIPLG SCTMKLNSSA
    560 570 580 590 600
    ELAPISWKEF ANIHPFVPLD QAQGYQQLFK DLEKDLCEIT GYDKISFQPN
    610 620 630 640 650
    SGAQGEYAGL AAIKAYLNAK GERHRSVCLI PRSAHGTNPA SAQMAGMKIQ
    660 670 680 690 700
    PIEVDKNGSI DISHLKAMVD KHKENLAAIM ITYPSTNGVF EEEIGDVCDL
    710 720 730 740 750
    IHKHGGQVYL DGANMNAQVG LCRPGDYGSD VSHLNLHKTF CIPHGGGGPG
    760 770 780 790 800
    MGPIGVKKHL APYLPTHPVI KIQTDKDACP LGTVSAAPWG SSAILPISWV
    810 820 830 840 850
    YIKTMGAKGL KHASEIAILN ANYMAKRLEK HYKILFRGVR GYVAHEFILD
    860 870 880 890 900
    TRPFKKTANI EAVDLAKRLQ DYGFHAPTMS WPVAGTLMIE PTESEDKGEL
    910 920 930 940 950
    DRFCDAMISI RQEIADIEEG RMDPQVNPLK MSPHTLNCVT SSKWDRPYSR
    960 970 980 990 1000
    EVAAFPLPFV KPESKFWPTI ARIDDIYGDQ HLVCTCPPME AYESPFSEQK

    RASS
    Length:1,004
    Mass (Da):111,852
    Last modified:May 1, 1992 - v2
    Checksum:i4446D7C66E0DC4BD
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M64402 mRNA. Translation: AAA49029.1.
    D90266 mRNA. Translation: BAA14313.1.
    D90240 Genomic DNA. Translation: BAA14287.1.
    PIRiA39521.
    RefSeqiNP_989653.1. NM_204322.1.
    UniGeneiGga.3393.

    Genome annotation databases

    GeneIDi374222.
    KEGGigga:374222.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M64402 mRNA. Translation: AAA49029.1.
    D90266 mRNA. Translation: BAA14313.1.
    D90240 Genomic DNA. Translation: BAA14287.1.
    PIRiA39521.
    RefSeqiNP_989653.1. NM_204322.1.
    UniGeneiGga.3393.

    3D structure databases

    ProteinModelPortaliP15505.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi9031.ENSGALP00000037556.

    Proteomic databases

    PaxDbiP15505.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    GeneIDi374222.
    KEGGigga:374222.

    Organism-specific databases

    CTDi2731.

    Phylogenomic databases

    eggNOGiCOG1003.
    HOGENOMiHOG000239369.
    HOVERGENiHBG005820.
    InParanoidiP15505.
    KOiK00281.
    PhylomeDBiP15505.

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-12926.
    BRENDAi1.4.4.2. 1306.

    Miscellaneous databases

    NextBioi20813724.
    PROiP15505.

    Family and domain databases

    Gene3Di3.40.640.10. 2 hits.
    HAMAPiMF_00711. GcvP.
    InterProiIPR001597. ArAA_b-elim_lyase/Thr_aldolase.
    IPR003437. GcvP.
    IPR020580. GDC-P_N.
    IPR020581. GDC_P.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    [Graphical view]
    PANTHERiPTHR11773. PTHR11773. 1 hit.
    PfamiPF01212. Beta_elim_lyase. 1 hit.
    PF02347. GDC-P. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 3 hits.
    TIGRFAMsiTIGR00461. gcvP. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    1. "The glycine cleavage system. Molecular cloning of the chicken and human glycine decarboxylase cDNAs and some characteristics involved in the deduced protein structures."
      Kume A., Koyata H., Sakakibara T., Ishiguro Y., Kure S., Hiraga K.
      J. Biol. Chem. 266:3323-3329(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Strain: White leghorn.
    2. "Amino acid sequence of the phosphopyridoxyl peptide from P-protein of the chicken liver glycine cleavage system."
      Fujiwara K., Okamura-Ikeda K., Motokawa Y.
      Biochem. Biophys. Res. Commun. 149:621-627(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 704-757.
      Tissue: Liver.
    3. "The mitochondrial glycine cleavage system. Purification and properties of glycine decarboxylase from chicken liver mitochondria."
      Hiraga K., Kikuchi G.
      J. Biol. Chem. 255:11664-11670(1980) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, ENZYME REGULATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, FUNCTION.
    4. "The mitochondrial glycine cleavage system. Functional association of glycine decarboxylase and aminomethyl carrier protein."
      Hiraga K., Kikuchi G.
      J. Biol. Chem. 255:11671-11676(1980) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, INTERACTION WITH GCSH, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, FUNCTION.

    Entry informationi

    Entry nameiGCSP_CHICK
    AccessioniPrimary (citable) accession number: P15505
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: May 1, 1992
    Last modified: April 29, 2015
    This is version 101 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.