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P15505

- GCSP_CHICK

UniProt

P15505 - GCSP_CHICK

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Protein

Glycine dehydrogenase (decarboxylating), mitochondrial

Gene
GLDC
Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

The glycine cleavage system catalyzes the degradation of glycine. The P protein (GLDC) binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein (GCSH).2 Publications

Catalytic activityi

Glycine + [glycine-cleavage complex H protein]-N(6)-lipoyl-L-lysine = [glycine-cleavage complex H protein]-S-aminomethyl-N(6)-dihydrolipoyl-L-lysine + CO2.2 Publications

Cofactori

Pyridoxal phosphate.1 Publication

Enzyme regulationi

Stimulated by lipoic acid. Inhibited in presence of methylamine.2 Publications

Kineticsi

  1. KM=20 mM for glycine in presence of GCSH (in 1 Publication)2 Publications
  2. KM=27 mM for methylamine in presence of GCSH (in 1 Publication)
  3. KM=40 mM for glycine (in 1 Publication)
  4. KM=3.5 mM for lipoic acid (in 1 Publication)
  5. KM=63 mM for methylamine (in 1 Publication)

GO - Molecular functioni

  1. glycine dehydrogenase (decarboxylating) activity Source: UniProtKB-EC
  2. lyase activity Source: InterPro
  3. pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  1. glycine catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12926.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine dehydrogenase (decarboxylating), mitochondrial (EC:1.4.4.2)
Alternative name(s):
Glycine cleavage system P protein
Glycine decarboxylase
Glycine dehydrogenase (aminomethyl-transferring)
Gene namesi
Name:GLDC
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Unplaced

Subcellular locationi

Mitochondrion 1 Publication

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 1004Glycine dehydrogenase (decarboxylating), mitochondrialUniRule annotationPRO_0000010742
Transit peptidei1 – ?Mitochondrion Reviewed prediction

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei738 – 7381N6-(pyridoxal phosphate)lysineUniRule annotation

Proteomic databases

PaxDbiP15505.

Expressioni

Tissue specificityi

Liver (at protein level).1 Publication

Interactioni

Subunit structurei

Homodimer. The glycine cleavage system is composed of four proteins: P (GLDC), T (GCST), L (DLD) and H (GCSH). Interacts with GCSH.2 Publications

Protein-protein interaction databases

STRINGi9031.ENSGALP00000037556.

Structurei

3D structure databases

ProteinModelPortaliP15505.

Family & Domainsi

Sequence similaritiesi

Belongs to the GcvP family.

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1003.
HOGENOMiHOG000239369.
HOVERGENiHBG005820.
KOiK00281.
PhylomeDBiP15505.

Family and domain databases

Gene3Di3.40.640.10. 2 hits.
HAMAPiMF_00711. GcvP.
InterProiIPR001597. ArAA_b-elim_lyase/Thr_aldolase.
IPR020580. GDC-P_N.
IPR020581. GDC_P.
IPR003437. GDC_P_homo.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERiPTHR11773. PTHR11773. 1 hit.
PfamiPF01212. Beta_elim_lyase. 1 hit.
PF02347. GDC-P. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 3 hits.
TIGRFAMsiTIGR00461. gcvP. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15505-1 [UniParc]FASTAAdd to Basket

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MQSCGRWWGR LAARGAPRHL RPAAGGPRRQ QQRWGGGEAA RCIEQLLPRH     50
DDFCRRHIGP REREKREMLS AVGVQSVEEL MDKTIPASIR LRRPLRMDDH 100
VVENEILETL YNIASKNKIW RSYIGMGYYN CSVPQPIARN LLENAGWVTQ 150
YTPYQPEVSQ GRLESLLNYQ TMVCDITGMD VANASLLDEG TAAAEAMQLC 200
HRQNKRRKFY IDARCHPQTI ANYTGVITEL KLPHEMDFSG KDVSGVLFQY 250
PDTEGKVEDF SELIERAHQN GTLACCATDL LALCILKPPG EFGVDVVLGS 300
SQRFGVPLCY GGPHAAFFAV KENLVRMMPG RMVGVTRDAN GKEVYRLALQ 350
TREQHIRRDK ATSNICTAQA LLANMAAMYG VYHGSDGLKD IARRVHNATL 400
ILAEGLRRAG HKLHHDLFFD TLTVTCGCSV KEVLDRAALR KINVRIYSDG 450
RLGVSLDETV NEKDLDDILW IFGCESSAEL VAEGMGEETK GILSTPFKRT 500
SKFLTHQVFN SYHSETNIVR YMKRLENKDI SLVHSMIPLG SCTMKLNSSA 550
ELAPISWKEF ANIHPFVPLD QAQGYQQLFK DLEKDLCEIT GYDKISFQPN 600
SGAQGEYAGL AAIKAYLNAK GERHRSVCLI PRSAHGTNPA SAQMAGMKIQ 650
PIEVDKNGSI DISHLKAMVD KHKENLAAIM ITYPSTNGVF EEEIGDVCDL 700
IHKHGGQVYL DGANMNAQVG LCRPGDYGSD VSHLNLHKTF CIPHGGGGPG 750
MGPIGVKKHL APYLPTHPVI KIQTDKDACP LGTVSAAPWG SSAILPISWV 800
YIKTMGAKGL KHASEIAILN ANYMAKRLEK HYKILFRGVR GYVAHEFILD 850
TRPFKKTANI EAVDLAKRLQ DYGFHAPTMS WPVAGTLMIE PTESEDKGEL 900
DRFCDAMISI RQEIADIEEG RMDPQVNPLK MSPHTLNCVT SSKWDRPYSR 950
EVAAFPLPFV KPESKFWPTI ARIDDIYGDQ HLVCTCPPME AYESPFSEQK 1000
RASS 1004
Length:1,004
Mass (Da):111,852
Last modified:May 1, 1992 - v2
Checksum:i4446D7C66E0DC4BD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M64402 mRNA. Translation: AAA49029.1.
D90266 mRNA. Translation: BAA14313.1.
D90240 Genomic DNA. Translation: BAA14287.1.
PIRiA39521.
RefSeqiNP_989653.1. NM_204322.1.
UniGeneiGga.3393.

Genome annotation databases

GeneIDi374222.
KEGGigga:374222.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M64402 mRNA. Translation: AAA49029.1 .
D90266 mRNA. Translation: BAA14313.1 .
D90240 Genomic DNA. Translation: BAA14287.1 .
PIRi A39521.
RefSeqi NP_989653.1. NM_204322.1.
UniGenei Gga.3393.

3D structure databases

ProteinModelPortali P15505.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9031.ENSGALP00000037556.

Proteomic databases

PaxDbi P15505.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 374222.
KEGGi gga:374222.

Organism-specific databases

CTDi 2731.

Phylogenomic databases

eggNOGi COG1003.
HOGENOMi HOG000239369.
HOVERGENi HBG005820.
KOi K00281.
PhylomeDBi P15505.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-12926.

Miscellaneous databases

NextBioi 20813724.
PROi P15505.

Family and domain databases

Gene3Di 3.40.640.10. 2 hits.
HAMAPi MF_00711. GcvP.
InterProi IPR001597. ArAA_b-elim_lyase/Thr_aldolase.
IPR020580. GDC-P_N.
IPR020581. GDC_P.
IPR003437. GDC_P_homo.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view ]
PANTHERi PTHR11773. PTHR11773. 1 hit.
Pfami PF01212. Beta_elim_lyase. 1 hit.
PF02347. GDC-P. 1 hit.
[Graphical view ]
SUPFAMi SSF53383. SSF53383. 3 hits.
TIGRFAMsi TIGR00461. gcvP. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The glycine cleavage system. Molecular cloning of the chicken and human glycine decarboxylase cDNAs and some characteristics involved in the deduced protein structures."
    Kume A., Koyata H., Sakakibara T., Ishiguro Y., Kure S., Hiraga K.
    J. Biol. Chem. 266:3323-3329(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: White leghorn.
  2. "Amino acid sequence of the phosphopyridoxyl peptide from P-protein of the chicken liver glycine cleavage system."
    Fujiwara K., Okamura-Ikeda K., Motokawa Y.
    Biochem. Biophys. Res. Commun. 149:621-627(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 704-757.
    Tissue: Liver.
  3. "The mitochondrial glycine cleavage system. Purification and properties of glycine decarboxylase from chicken liver mitochondria."
    Hiraga K., Kikuchi G.
    J. Biol. Chem. 255:11664-11670(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, ENZYME REGULATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, FUNCTION.
  4. "The mitochondrial glycine cleavage system. Functional association of glycine decarboxylase and aminomethyl carrier protein."
    Hiraga K., Kikuchi G.
    J. Biol. Chem. 255:11671-11676(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, INTERACTION WITH GCSH, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, FUNCTION.

Entry informationi

Entry nameiGCSP_CHICK
AccessioniPrimary (citable) accession number: P15505
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: May 1, 1992
Last modified: April 16, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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