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P15505

- GCSP_CHICK

UniProt

P15505 - GCSP_CHICK

Protein

Glycine dehydrogenase (decarboxylating), mitochondrial

Gene

GLDC

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 2 (01 May 1992)
      Previous versions | rss
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    Functioni

    The glycine cleavage system catalyzes the degradation of glycine. The P protein (GLDC) binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein (GCSH).2 Publications

    Catalytic activityi

    Glycine + [glycine-cleavage complex H protein]-N(6)-lipoyl-L-lysine = [glycine-cleavage complex H protein]-S-aminomethyl-N(6)-dihydrolipoyl-L-lysine + CO2.2 Publications

    Cofactori

    Pyridoxal phosphate.1 Publication

    Enzyme regulationi

    Stimulated by lipoic acid. Inhibited in presence of methylamine.2 Publications

    Kineticsi

    1. KM=20 mM for glycine in presence of GCSH (in PubMed:7440563)2 Publications
    2. KM=27 mM for methylamine in presence of GCSH (in PubMed:7440563)2 Publications
    3. KM=40 mM for glycine (in PubMed:7440562)2 Publications
    4. KM=3.5 mM for lipoic acid (in PubMed:7440562)2 Publications
    5. KM=63 mM for methylamine (in PubMed:7440562)2 Publications

    GO - Molecular functioni

    1. glycine dehydrogenase (decarboxylating) activity Source: UniProtKB-EC
    2. lyase activity Source: InterPro
    3. pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    1. glycine catabolic process Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-12926.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycine dehydrogenase (decarboxylating), mitochondrial (EC:1.4.4.2)
    Alternative name(s):
    Glycine cleavage system P protein
    Glycine decarboxylase
    Glycine dehydrogenase (aminomethyl-transferring)
    Gene namesi
    Name:GLDC
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Unplaced

    Subcellular locationi

    Mitochondrion 1 Publication

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 1004Glycine dehydrogenase (decarboxylating), mitochondrialPRO_0000010742
    Transit peptidei1 – ?MitochondrionSequence Analysis

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei738 – 7381N6-(pyridoxal phosphate)lysine

    Proteomic databases

    PaxDbiP15505.

    Expressioni

    Tissue specificityi

    Liver (at protein level).1 Publication

    Interactioni

    Subunit structurei

    Homodimer. The glycine cleavage system is composed of four proteins: P (GLDC), T (GCST), L (DLD) and H (GCSH). Interacts with GCSH.2 Publications

    Protein-protein interaction databases

    STRINGi9031.ENSGALP00000037556.

    Structurei

    3D structure databases

    ProteinModelPortaliP15505.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GcvP family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1003.
    HOGENOMiHOG000239369.
    HOVERGENiHBG005820.
    KOiK00281.
    PhylomeDBiP15505.

    Family and domain databases

    Gene3Di3.40.640.10. 2 hits.
    HAMAPiMF_00711. GcvP.
    InterProiIPR001597. ArAA_b-elim_lyase/Thr_aldolase.
    IPR020580. GDC-P_N.
    IPR020581. GDC_P.
    IPR003437. GDC_P_homo.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    [Graphical view]
    PANTHERiPTHR11773. PTHR11773. 1 hit.
    PfamiPF01212. Beta_elim_lyase. 1 hit.
    PF02347. GDC-P. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 3 hits.
    TIGRFAMsiTIGR00461. gcvP. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P15505-1 [UniParc]FASTAAdd to Basket

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    MQSCGRWWGR LAARGAPRHL RPAAGGPRRQ QQRWGGGEAA RCIEQLLPRH     50
    DDFCRRHIGP REREKREMLS AVGVQSVEEL MDKTIPASIR LRRPLRMDDH 100
    VVENEILETL YNIASKNKIW RSYIGMGYYN CSVPQPIARN LLENAGWVTQ 150
    YTPYQPEVSQ GRLESLLNYQ TMVCDITGMD VANASLLDEG TAAAEAMQLC 200
    HRQNKRRKFY IDARCHPQTI ANYTGVITEL KLPHEMDFSG KDVSGVLFQY 250
    PDTEGKVEDF SELIERAHQN GTLACCATDL LALCILKPPG EFGVDVVLGS 300
    SQRFGVPLCY GGPHAAFFAV KENLVRMMPG RMVGVTRDAN GKEVYRLALQ 350
    TREQHIRRDK ATSNICTAQA LLANMAAMYG VYHGSDGLKD IARRVHNATL 400
    ILAEGLRRAG HKLHHDLFFD TLTVTCGCSV KEVLDRAALR KINVRIYSDG 450
    RLGVSLDETV NEKDLDDILW IFGCESSAEL VAEGMGEETK GILSTPFKRT 500
    SKFLTHQVFN SYHSETNIVR YMKRLENKDI SLVHSMIPLG SCTMKLNSSA 550
    ELAPISWKEF ANIHPFVPLD QAQGYQQLFK DLEKDLCEIT GYDKISFQPN 600
    SGAQGEYAGL AAIKAYLNAK GERHRSVCLI PRSAHGTNPA SAQMAGMKIQ 650
    PIEVDKNGSI DISHLKAMVD KHKENLAAIM ITYPSTNGVF EEEIGDVCDL 700
    IHKHGGQVYL DGANMNAQVG LCRPGDYGSD VSHLNLHKTF CIPHGGGGPG 750
    MGPIGVKKHL APYLPTHPVI KIQTDKDACP LGTVSAAPWG SSAILPISWV 800
    YIKTMGAKGL KHASEIAILN ANYMAKRLEK HYKILFRGVR GYVAHEFILD 850
    TRPFKKTANI EAVDLAKRLQ DYGFHAPTMS WPVAGTLMIE PTESEDKGEL 900
    DRFCDAMISI RQEIADIEEG RMDPQVNPLK MSPHTLNCVT SSKWDRPYSR 950
    EVAAFPLPFV KPESKFWPTI ARIDDIYGDQ HLVCTCPPME AYESPFSEQK 1000
    RASS 1004
    Length:1,004
    Mass (Da):111,852
    Last modified:May 1, 1992 - v2
    Checksum:i4446D7C66E0DC4BD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M64402 mRNA. Translation: AAA49029.1.
    D90266 mRNA. Translation: BAA14313.1.
    D90240 Genomic DNA. Translation: BAA14287.1.
    PIRiA39521.
    RefSeqiNP_989653.1. NM_204322.1.
    UniGeneiGga.3393.

    Genome annotation databases

    GeneIDi374222.
    KEGGigga:374222.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M64402 mRNA. Translation: AAA49029.1 .
    D90266 mRNA. Translation: BAA14313.1 .
    D90240 Genomic DNA. Translation: BAA14287.1 .
    PIRi A39521.
    RefSeqi NP_989653.1. NM_204322.1.
    UniGenei Gga.3393.

    3D structure databases

    ProteinModelPortali P15505.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9031.ENSGALP00000037556.

    Proteomic databases

    PaxDbi P15505.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 374222.
    KEGGi gga:374222.

    Organism-specific databases

    CTDi 2731.

    Phylogenomic databases

    eggNOGi COG1003.
    HOGENOMi HOG000239369.
    HOVERGENi HBG005820.
    KOi K00281.
    PhylomeDBi P15505.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-12926.

    Miscellaneous databases

    NextBioi 20813724.
    PROi P15505.

    Family and domain databases

    Gene3Di 3.40.640.10. 2 hits.
    HAMAPi MF_00711. GcvP.
    InterProi IPR001597. ArAA_b-elim_lyase/Thr_aldolase.
    IPR020580. GDC-P_N.
    IPR020581. GDC_P.
    IPR003437. GDC_P_homo.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    [Graphical view ]
    PANTHERi PTHR11773. PTHR11773. 1 hit.
    Pfami PF01212. Beta_elim_lyase. 1 hit.
    PF02347. GDC-P. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 3 hits.
    TIGRFAMsi TIGR00461. gcvP. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The glycine cleavage system. Molecular cloning of the chicken and human glycine decarboxylase cDNAs and some characteristics involved in the deduced protein structures."
      Kume A., Koyata H., Sakakibara T., Ishiguro Y., Kure S., Hiraga K.
      J. Biol. Chem. 266:3323-3329(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Strain: White leghorn.
    2. "Amino acid sequence of the phosphopyridoxyl peptide from P-protein of the chicken liver glycine cleavage system."
      Fujiwara K., Okamura-Ikeda K., Motokawa Y.
      Biochem. Biophys. Res. Commun. 149:621-627(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 704-757.
      Tissue: Liver.
    3. "The mitochondrial glycine cleavage system. Purification and properties of glycine decarboxylase from chicken liver mitochondria."
      Hiraga K., Kikuchi G.
      J. Biol. Chem. 255:11664-11670(1980) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, ENZYME REGULATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, FUNCTION.
    4. "The mitochondrial glycine cleavage system. Functional association of glycine decarboxylase and aminomethyl carrier protein."
      Hiraga K., Kikuchi G.
      J. Biol. Chem. 255:11671-11676(1980) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, INTERACTION WITH GCSH, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, FUNCTION.

    Entry informationi

    Entry nameiGCSP_CHICK
    AccessioniPrimary (citable) accession number: P15505
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 95 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3