P15494 (BEV1A_BETPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Major pollen allergen Bet v 1-A

Alternative name(s):
Allergen Bet v I-A
Allergen=Bet v 1-A

Gene names

Name:	BETVIA
Synonyms:	BETVI

Organism

Betula pendula (European white birch) (Betula verrucosa)

Taxonomic identifier

3505 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fagales › Betulaceae › Betula

Protein attributes

Sequence length	160 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be a general steroid carrier protein By similarity.
Subcellular location	Cytoplasm.
Allergenic properties	Causes an allergic reaction in human. Is a cause of type I allergic reactions in Europe, North America and USSR.
Sequence similarities	Belongs to the BetVI family.

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Cytoplasm
Disease	Allergen
Molecular function	Pathogenesis-related protein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	defense response Inferred from electronic annotation. Source: UniProtKB-KW response to biotic stimulus Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.1 Ref.3

Chain

2 – 160

159

Major pollen allergen Bet v 1-A

PRO_0000154174

Sites

Binding site

70

1

Substrate By similarity

Binding site

95

1

Substrate By similarity

Binding site

121

1

Substrate By similarity

Binding site

127

1

Substrate By similarity

Natural variations

Natural variant

63

1

F → L.

Experimental info

Mutagenesis

29

1

N → T: Slightly reduced interaction with BV16; when associated with N-33. Ref.8

Mutagenesis

33

1

K → N: Slightly reduced interaction with BV16; when associated with T-29. Ref.8

Mutagenesis

46

1

E → S: Strongly reduced interaction with BV16, hypoallergenic. Ref.8

Mutagenesis

48

1

N → S: Slightly reduced interaction with BV16. Ref.8

Mutagenesis

61

1

E → S: Slightly reduced interaction with BV16. Ref.8

Mutagenesis

109

1

P → G: Slightly reduced interaction with BV16. Ref.8

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

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.

.

.

.

.

160

Helix Strand Turn

Details...

Beta strand

3 – 14

12

Helix

16 – 23

8

Turn

24 – 26

3

Helix

27 – 34

8

Turn

36 – 38

3

Beta strand

41 – 46

6

Beta strand

48 – 50

3

Beta strand

54 – 58

5

Beta strand

61 – 64

4

Beta strand

66 – 76

11

Turn

77 – 80

4

Beta strand

81 – 89

9

Beta strand

93 – 95

3

Beta strand

96 – 107

12

Turn

109 – 111

3

Beta strand

113 – 125

13

Helix

131 – 154

24

Turn

156 – 159

4

Sequences

	Sequence		Length	Mass (Da)
	P15494 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 69B44110BBDA1AC6	FASTA	160	17,571
	10 20 30 40 50 60 MGVFNYETET TSVIPAARLF KAFILDGDNL FPKVAPQAIS SVENIEGNGG PGTIKKISFP 70 80 90 100 110 120 EGFPFKYVKD RVDEVDHTNF KYNYSVIEGG PIGDTLEKIS NEIKIVATPD GGSILKISNK 130 140 150 160 YHTKGDHEVK AEQVKASKEM GETLLRAVES YLLAHSDAYN

References

[1]	"The gene coding for the major birch pollen allergen Betv1, is highly homologous to a pea disease resistance response gene." Breiteneder H., Pettenburger K., Bito A., Valenta R., Kraft D., Rumpold H., Scheiner O., Breitenbach M. EMBO J. 8:1935-1938(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-35. Tissue: Pollen.
[2]	Larsen J.N. Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pollen.
[3]	"Isoforms of Bet v 1, the major birch pollen allergen, analyzed by liquid chromatography, mass spectrometry, and cDNA cloning." Swoboda I., Jilek A., Ferreira F., Engel E., Hoffman-Sommergruber K., Scheiner O., Kraft D., Breiteneder H., Pittenauer E., Schmid E., Vicente O., Heberle-Bors E., Ahorn H., Breitenbach M. J. Biol. Chem. 270:2607-2613(1995) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-160. Tissue: Pollen.
[4]	"Purification and N-terminal amino acid sequence of two birch pollen isoallergens (Bet v I and Bet v II)." Elsayed S., Vik H. Int. Arch. Allergy Appl. Immunol. 93:378-384(1990) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE.
[5]	"Secondary structure and tertiary fold of the birch pollen allergen Bet v 1 in solution." Faber C., Lindemann A., Sticht H., Ejchart A., Kungl A., Susani M., Frank R.W., Kraft D., Breitenbach M., Roesch P. J. Biol. Chem. 271:19243-19250(1996) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.
[6]	"X-ray and NMR structure of Bet v 1, the origin of birch pollen allergy." Gajhede M., Osmark P., Poulsen F.M., Ipsen H., Larsen J.N., van Neerven R.J.J., Schou C., Loewenstein H., Spangfort M.D. Nat. Struct. Biol. 3:1040-1045(1996) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), STRUCTURE BY NMR.
[7]	"Dominant epitopes and allergic cross-reactivity: complex formation between a Fab fragment of a monoclonal murine IgG antibody and the major allergen from birch pollen Bet v 1." Mirza O., Henriksen A., Ipsen H., Larsen J.N., Wissenbach M., Spangfort M.D., Gajhede M. J. Immunol. 165:331-338(2000) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) IN COMPLEX WITH THE FAB FRAGMENT FROM A MURINE MONOCLONAL IGG1 AB.
[8]	"Dominating IgE-binding epitope of Bet v 1, the major allergen of birch pollen, characterized by X-ray crystallography and site-directed mutagenesis." Spangfort M.D., Mirza O., Ipsen H., Van Neerven R.J., Gajhede M., Larsen J.N. J. Immunol. 171:3084-3090(2003) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) IN COMPLEX WITH THE FAB FRAGMENT FROM THE HUMAN MONOCLONAL BV16 IMMUNOGLOBULIN, MUTAGENESIS OF ASN-29; LYS-33; GLU-46; ASN-48; GLU-61 AND PRO-109.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X15877 mRNA. Translation: CAA33887.1.
Z80098 mRNA. Translation: CAB02153.1.
Z80099 mRNA. Translation: CAB02154.1.
Z80104 mRNA. Translation: CAB02159.1.

PIR

S05376.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1B6F	NMR	-	A	2-159	[»]
1BTV	NMR	-	A	2-160	[»]
1BV1	X-ray	2.00	A	2-160	[»]
1FSK	X-ray	2.90	A/D/G/J	2-160	[»]
1LLT	X-ray	3.10	A	2-160	[»]
1QMR	X-ray	2.15	A	2-160	[»]
4A80	X-ray	1.96	A	2-160	[»]
4A81	X-ray	2.05	A	2-160	[»]
4A83	X-ray	1.54	A	2-160	[»]
4A84	X-ray	1.50	A	2-160	[»]
4A85	X-ray	1.40	A	2-160	[»]
4A86	X-ray	1.59	A	2-160	[»]
4A87	X-ray	1.24	A	2-160	[»]
4A88	X-ray	1.51	A	2-160	[»]
4A8G	X-ray	2.10	A	2-160	[»]

ProteinModelPortal

P15494.

SMR

P15494. Positions 2-160.

ModBase

Search...

Protein family/group databases

Allergome

123. Bet v 1.2801.
89. Bet v 1.
90. Bet v 1.0101.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

3.30.530.20. 1 hit.

InterPro

IPR024949. Bet_v_I_allergen.
IPR000916. Bet_v_I_dom.
IPR023393. START-like_dom.
[Graphical view]

Pfam

PF00407. Bet_v_1. 1 hit.
[Graphical view]

PRINTS

PR00634. BETALLERGEN.

PROSITE

PS00451. PATHOGENESIS_BETVI. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P15494.

Entry information

Entry name

BEV1A_BETPN

Accession

Primary (citable) accession number: P15494
Secondary accession number(s): Q96369

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program