Reviewed, UniProtKB/Swiss-Prot P15494 (BEV1A_BETVE)

Names and origin

Protein names

Recommended name:
    Major pollen allergen Bet v 1-A
Alternative name(s):
    Allergen Bet v I-A
    Allergen=Bet v 1-A

Gene names

Name:	BETVIA
Synonyms:	BETVI

Organism

Betula verrucosa (White birch) (Betula pendula)

Taxonomic identifier

3505 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fagales › Betulaceae › Betula

Protein attributes

Sequence length	160 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May be a general steroid carrier protein By similarity.
Subcellular location	Cytoplasm.
Allergenic properties	Causes an allergic reaction in human. Is a cause of type I allergic reactions in Europe, North America and USSR.
Sequence similarities	Belongs to the BetVI family.

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Cytoplasm
Disease	Allergen
Molecular function	Pathogenesis-related protein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: InterPro response to biotic stimulus Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed

Chain

2 – 160

159

Major pollen allergen Bet v 1-A

PRO_0000154174

Sites

Binding site

70

1

Substrate By similarity

Binding site

95

1

Substrate By similarity

Binding site

121

1

Substrate By similarity

Binding site

127

1

Substrate By similarity

Natural variations

Natural variant

63

1

F → L

Experimental info

Mutagenesis

29

1

N → T: Slightly reduced interaction with BV16; when associated with N-33

Mutagenesis

33

1

K → N: Slightly reduced interaction with BV16; when associated with T-29

Mutagenesis

46

1

E → S: Strongly reduced interaction with BV16, hypoallergenic

Mutagenesis

48

1

N → S: Slightly reduced interaction with BV16

Mutagenesis

61

1

E → S: Slightly reduced interaction with BV16

Mutagenesis

109

1

P → G: Slightly reduced interaction with BV16

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

160

Helix Strand Turn

Details...

Beta strand

3 – 14

12

Helix

16 – 23

8

Turn

24 – 26

3

Helix

27 – 34

8

Turn

36 – 38

3

Beta strand

40 – 50

11

Beta strand

54 – 58

5

Beta strand

66 – 76

11

Turn

77 – 80

4

Beta strand

81 – 89

9

Beta strand

96 – 107

12

Beta strand

113 – 124

12

Helix

131 – 154

24

Turn

156 – 159

4

Sequences

	Sequence		Length	Mass (Da)
	P15494-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 69B44110BBDA1AC6	FASTA	160	17,571
	10 20 30 40 50 60 MGVFNYETET TSVIPAARLF KAFILDGDNL FPKVAPQAIS SVENIEGNGG PGTIKKISFP 70 80 90 100 110 120 EGFPFKYVKD RVDEVDHTNF KYNYSVIEGG PIGDTLEKIS NEIKIVATPD GGSILKISNK 130 140 150 160 YHTKGDHEVK AEQVKASKEM GETLLRAVES YLLAHSDAYN

References

[1]	"The gene coding for the major birch pollen allergen Betv1, is highly homologous to a pea disease resistance response gene." Breiteneder H., Pettenburger K., Bito A., Valenta R., Kraft D., Rumpold H., Scheiner O., Breitenbach M. EMBO J. 8:1935-1938(1989) [PubMed: 2571499] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-35. Tissue: Pollen.
[2]	Larsen J.N. Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pollen.
[3]	"Isoforms of Bet v 1, the major birch pollen allergen, analyzed by liquid chromatography, mass spectrometry, and cDNA cloning." Swoboda I., Jilek A., Ferreira F., Engel E., Hoffman-Sommergruber K., Scheiner O., Kraft D., Breiteneder H., Pittenauer E., Schmid E., Vicente O., Heberle-Bors E., Ahorn H., Breitenbach M. J. Biol. Chem. 270:2607-2613(1995) [PubMed: 7852325] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-160. Tissue: Pollen.
[4]	"Purification and N-terminal amino acid sequence of two birch pollen isoallergens (Bet v I and Bet v II)." Elsayed S., Vik H. Int. Arch. Allergy Appl. Immunol. 93:378-384(1990) [PubMed: 2101127] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE.
[5]	"Secondary structure and tertiary fold of the birch pollen allergen Bet v 1 in solution." Faber C., Lindemann A., Sticht H., Ejchart A., Kungl A., Susani M., Frank R.W., Kraft D., Breitenbach M., Roesch P. J. Biol. Chem. 271:19243-19250(1996) [PubMed: 8702605] [Abstract] Cited for: STRUCTURE BY NMR.
[6]	"X-ray and NMR structure of Bet v 1, the origin of birch pollen allergy." Gajhede M., Osmark P., Poulsen F.M., Ipsen H., Larsen J.N., van Neerven R.J.J., Schou C., Loewenstein H., Spangfort M.D. Nat. Struct. Biol. 3:1040-1045(1996) [PubMed: 8946858] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), STRUCTURE BY NMR.
[7]	"Dominant epitopes and allergic cross-reactivity: complex formation between a Fab fragment of a monoclonal murine IgG antibody and the major allergen from birch pollen Bet v 1." Mirza O., Henriksen A., Ipsen H., Larsen J.N., Wissenbach M., Spangfort M.D., Gajhede M. J. Immunol. 165:331-338(2000) [PubMed: 10861069] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) IN COMPLEX WITH THE FAB FRAGMENT FROM A MURINE MONOCLONAL IGG1 AB.
[8]	"Dominating IgE-binding epitope of Bet v 1, the major allergen of birch pollen, characterized by X-ray crystallography and site-directed mutagenesis." Spangfort M.D., Mirza O., Ipsen H., Van Neerven R.J., Gajhede M., Larsen J.N. J. Immunol. 171:3084-3090(2003) [PubMed: 12960334] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) IN COMPLEX WITH THE FAB FRAGMENT FROM THE HUMAN MONOCLONAL BV16 IMMUNOGLOBULIN, MUTAGENESIS OF ASN-29; LYS-33; GLU-46; ASN-48; GLU-61 AND PRO-109.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X15877 mRNA. Translation: CAA33887.1.
Z80098 mRNA. Translation: CAB02153.1.
Z80099 mRNA. Translation: CAB02154.1.
Z80104 mRNA. Translation: CAB02159.1.

PIR

S05376.

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1B6F	NMR	-	A	1-160	[»]
1BTV	NMR	-	A	1-160	[»]
1BV1	X-ray	2.00	A	1-160	[»]
1FSK	X-ray	2.90	A/D/G/J	1-160	[»]
1LLT	X-ray	3.10	A	1-160	[»]
1QMR	X-ray	2.15	A	1-160	[»]

ModBase

Search...

Family and domain databases

InterPro

IPR000916. Bet_v_I.
[Graphical view]

Pfam

PF00407. Bet_v_I. 1 hit.
[Graphical view]

ProDom

PD000531. Bet_v_I. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS00451. PATHOGENESIS_BETVI. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

LinkHub

P15494.

Entry information

Entry name

BEV1A_BETVE

Accession

Primary (citable) accession number: P15494
Secondary accession number(s): Q96369

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)