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Protein

Isocitrate lyase

Gene
N/A
Organism
Ricinus communis (Castor bean)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in storage lipid mobilization during the growth of higher plant seedling.By similarity

Catalytic activityi

Isocitrate = succinate + glyoxylate.By similarity

Cofactori

Mg2+By similarity

Pathwayi: glyoxylate cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from isocitrate.By similarity
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Isocitrate lyase
  2. Malate synthase (RCOM_1506900), Malate synthase, glyoxysomal
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi175MagnesiumBy similarity1
Active sitei213Proton acceptorBy similarity1
Binding sitei250SubstrateBy similarity1
Binding sitei472SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlyoxylate bypass, Tricarboxylic acid cycle
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00719.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate lyaseBy similarity (EC:4.1.3.1By similarity)
Short name:
ICLBy similarity
Alternative name(s):
IsocitraseBy similarity
IsocitratsysaseBy similarity
OrganismiRicinus communis (Castor bean)
Taxonomic identifieri3988 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesEuphorbiaceaeAcalyphoideaeAcalypheaeRicinus

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Glyoxysome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000688101 – 576Isocitrate lyaseAdd BLAST576

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP15479.
SMRiP15479.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni104 – 106Substrate bindingBy similarity3
Regioni214 – 215Substrate bindingBy similarity2
Regioni437 – 441Substrate bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi574 – 576Microbody targeting signalSequence analysis3

Sequence similaritiesi

Phylogenomic databases

KOiK01637.

Family and domain databases

InterProiView protein in InterPro
IPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
PANTHERiPTHR21631. PTHR21631. 1 hit.
PfamiView protein in Pfam
PF00463. ICL. 1 hit.
PIRSFiPIRSF001362. Isocit_lyase. 1 hit.
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 1 hit.
PROSITEiView protein in PROSITE
PS00161. ISOCITRATE_LYASE. 1 hit.

Sequencei

Sequence statusi: Complete.

P15479-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASFSGPSM IMEEEGRFEA EVAEVQAWWN SERFKLTRRP YTARDVVALR
60 70 80 90 100
GNLKQSYASN ELAKKLWRTL KTHQANGTAS RTFGALDPVQ VTMMAKHLDS
110 120 130 140 150
IYVSGWQCSS THTTTNEPGP DLADYPYDTV PNKVEHLFFA QQYHDRKQRE
160 170 180 190 200
ARMSMSREER ARTPYVDYLK PIIADGDTGF GGTTATVKLC KLFVERGAAG
210 220 230 240 250
VHIEDQSSVT KKCGHMAGKV LVAISEHINR LVAARLQFDV MGVETLLVAR
260 270 280 290 300
TDAEAANLIQ SNVDTRDHQF ILGVTNPNLR GKSLATLLAT GMANGKTGAE
310 320 330 340 350
LQATEDNWLA MAQLKTFPEC VMDAIKNMNA GEDEKRRRMN EWMNHTSYDK
360 370 380 390 400
CLSYEQGREI ADRMGLKNLF WDWDLPRTRE GFYRFKGSVM AAVVRGRAFA
410 420 430 440 450
PHADIIWMET AKPDFAECTA FAEGVKSMHP EIMLAYNLSP SFNWDASGMT
460 470 480 490 500
DEQMRDFIPR IARLGFCWQF ITLGGFHADA LVIDTFAKDY ARRGMLAYVE
510 520 530 540 550
RIQREERKNG VDTLAHQKWS GANYYDRYLK TVQGGISSTA AMGKGVTEEQ
560 570
FKETWTRPGA MEMGSAGSEV VAKARM
Length:576
Mass (Da):64,752
Last modified:April 1, 1990 - v1
Checksum:i8177A7679050579B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17145 mRNA. Translation: AAA53378.1.
PIRiS06274. WZCSI.
RefSeqiNP_001310641.1. NM_001323712.1.

Genome annotation databases

GeneIDi8264280.
KEGGircu:8264280.

Similar proteinsi

Entry informationi

Entry nameiACEA_RICCO
AccessioniPrimary (citable) accession number: P15479
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: August 30, 2017
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families