P15476 (PATB1_SOLTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Patatin-B1
EC=3.1.1.-

Gene names

Name:

PATB1

Organism

Solanum tuberosum (Potato)

Taxonomic identifier

4113 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum

Protein attributes

Sequence length	386 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Probable lipolytic acyl hydrolase (LAH), an activity which is thought to be involved in the response of tubers to pathogens.
Subcellular location	Vacuole By similarity.
Domain	The nitrogen atoms of the two glycine residues in the GGXR motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.
Miscellaneous	Patatin have a dual role as a somatic storage protein and as an enzyme involved in host resistance. This tuber protein represents approximately 40% of the total protein in mature tubers.
Sequence similarities	Belongs to the patatin family. Contains 1 patatin domain.

Ontologies

Keywords
Biological process	Lipid degradation Lipid metabolism Plant defense
Cellular component	Vacuole
Domain	Signal
Molecular function	Hydrolase Storage protein
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW nutrient reservoir activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23
	Chain	24 – 386	363	Patatin-B1		PRO_0000032257
Regions
	Domain	32 – 228	197	Patatin
	Motif	37 – 40	4	GGXR
	Motif	75 – 79	5	GXSXG
Sites
	Active site	77	1	By similarity
	Active site	215	1	By similarity
Amino acid modifications
	Glycosylation	115	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	290	1	L → M in AAA33828. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P15476 [UniParc]. Last modified July 24, 2007. Version 2. Checksum: F220B96112D8C665	FASTA	386	42,609
	10 20 30 40 50 60 MATTKSFLIL FFMILATTSS TCAKLEEMVT VLSIDGGGIK GIIPAIILEF LEGQLQEVDN 70 80 90 100 110 120 NKDARLADYF DVIGGTSTGG LLTAMITTPN ENNRPFAAAK DIVPFYFEHG PHIFNYSGSI 130 140 150 160 170 180 FGPRYDGKYL LQVLQEKLGE TRVHQALTEV AISSFDIKTN KPVIFTKSNL AKSPELDAKM 190 200 210 220 230 240 YDICYSIAAA PIYFPPHHFV THTSNGATYE FNLVDGGVAT VGDPALLSLS VATRLAQEDP 250 260 270 280 290 300 AFSSIKSLDY KQMLLLSLGT GTNSEFDKTY TAEEAAKWGP LRWMLAIQQL TNAASSYMTD 310 320 330 340 350 360 YYISTVFQAR HSQNNYLRVQ ENALTGTTTE MDDASEANME LLVQVGETLL KKPVSKDSPE 370 380 TYEEALKRFA KLLSNRKKLR ANKASY

References

[1]	"Molecular cloning and analysis of four potato tuber mRNAs." Stiekema W.J., Heidekamp F., Dirkse W.G., van Beckum J., de Haan P., ten Bosch C., Louwerse J.D. Plant Mol. Biol. 11:255-269(1988) [Agricola: IND92000050] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Bintje.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M21879 mRNA. Translation: AAA33828.1. X13179 mRNA. Translation: CAA31576.1.
PIR	B26017. S05593.
3D structure databases
ProteinModelPortal	P15476.
SMR	P15476. Positions 23-383.
ModBase	Search...
Protein family/group databases
Allergome	3489. Sola t 1.0101. 639. Sola t 1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR016035. Acyl_Trfase/lysoPLipase. IPR002641. Patatin/PLipase_A2-rel. [Graphical view]
Pfam	PF01734. Patatin. 1 hit. [Graphical view]
SUPFAM	SSF52151. Acyl_Trfase/lysoPlipase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PATB1_SOLTU

Accession

Primary (citable) accession number: P15476
Secondary accession number(s): Q41467

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	July 24, 2007
Last modified:	October 19, 2011
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program