P15473 (IBP3_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Insulin-like growth factor-binding protein 3
Short name=IBP-3
Short name=IGF-binding protein 3
Short name=IGFBP-3

Gene names

Name:	Igfbp3
Synonyms:	Igfbp-3

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.
Subunit structure	Interacts with XLKD1. Binds IGF2 more than IGF1. Forms a ternary complex of about 140 to 150 kDa with IGF1 or IGF2 and a 85 kDa glycoprotein (ALS). Interacts with HN. Interacts with TMEM219 By similarity.
Subcellular location	Secreted.
Post-translational modification	Phosphorylation sites are present in the extracellular medium By similarity.
Sequence similarities	Contains 1 IGFBP N-terminal domain. Contains 1 thyroglobulin type-1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Growth factor binding
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of apoptotic process Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of myoblast differentiation Inferred from sequence or structural similarity. Source: UniProtKB regulation of cell growth Inferred from electronic annotation. Source: InterPro
Cellular_component	insulin-like growth factor binary complex Inferred from direct assay PubMed 9346943. Source: RGD insulin-like growth factor ternary complex Inferred from direct assay PubMed 9346943. Source: RGD
Molecular_function	insulin-like growth factor binding Traceable author statement PubMed 11572795. Source: RGD protein tyrosine phosphatase activator activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Ref.3 Ref.4 Ref.5
	Chain	28 – 292	265	Insulin-like growth factor-binding protein 3		PRO_0000014380
Regions
	Domain	36 – 119	84	IGFBP N-terminal
	Domain	211 – 286	76	Thyroglobulin type-1
Amino acid modifications
	Glycosylation	118	1	N-linked (GlcNAc...) Potential
	Glycosylation	124	1	N-linked (GlcNAc...) Potential
	Glycosylation	137	1	N-linked (GlcNAc...) Potential
	Glycosylation	200	1	N-linked (GlcNAc...) Potential
	Disulfide bond	214 ↔ 241		By similarity
	Disulfide bond	252 ↔ 263		By similarity
	Disulfide bond	265 ↔ 286		By similarity
Experimental info
	Sequence conflict	8	1	Missing in AAB00989. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P15473 [UniParc]. Last modified August 1, 1990. Version 2. Checksum: 1887D5E2A528F3B2	FASTA	292	31,680
	10 20 30 40 50 60 MHPARPALWA AALTALTLLR GPPVARAGAG AVGAGPVVRC EPCDARALAQ CAPPPTAPAC 70 80 90 100 110 120 TELVREPGCG CCLTCALREG DACGVYTERC GTGLRCQPRP AEQYPLKALL NGRGFCANAS 130 140 150 160 170 180 AASNLSAYLP SQPSPGNTTE SEEDHNAGSV ESQVVPSTHR VTDSKFHPLH SKMEVIIKGQ 190 200 210 220 230 240 ARDSQRYKVD YESQSTDTQN FSSESKRETE YGPCRREMED TLNHLKFLNV LSPRGVHIPN 250 260 270 280 290 CDKKGFYKKK QCRPSKGRKR GFCWCVDKYG QPLPGYDTKG KDDVHCLSVQ SQ

References

[1]	"Complementary DNA structure of the high molecular weight rat insulin-like growth factor binding protein (IGF-BP3) and tissue distribution of its mRNA." Shimasaki S., Koba A., Mercado M., Shimonaka M., Ling N. Biochem. Biophys. Res. Commun. 165:907-912(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning and characterization of the growth hormone-dependent insulin-like growth factor binding protein (IGFBP-3) in the rat." Albiston A.L., Herington A.C. Biochem. Biophys. Res. Commun. 166:892-897(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[3]	"Isolation and NH2-terminal amino acid sequences of rat serum carrier proteins for insulin-like growth factors." Zapf J., Born W., Chang J.Y., James P., Froesch E.R., Fischer J.A. Biochem. Biophys. Res. Commun. 156:1187-1194(1988) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 28-68.
[4]	"Binding proteins for insulin-like growth factors in adult rat serum. Comparison with other human and rat binding proteins." Baxter R.C., Martin J.L. Biochem. Biophys. Res. Commun. 147:408-415(1987) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 28-42.
[5]	"Identification of a novel binding protein for insulin-like growth factors in adult rat serum." Shimonaka M., Schroeder R., Shimasaki S., Ling N. Biochem. Biophys. Res. Commun. 165:189-195(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 28-49. Tissue: Serum.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M31837 mRNA. Translation: AAA41383.1. M33300 mRNA. Translation: AAB00989.1.
IPI	IPI00209369.
PIR	A36748.
RefSeq	NP_036720.2. NM_012588.2.
UniGene	Rn.26369.
3D structure databases
ProteinModelPortal	P15473.
SMR	P15473. Positions 38-118.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000011678.
Protein family/group databases
MEROPS	I31.952.
PTM databases
PhosphoSite	P15473.
Proteomic databases
PaxDb	P15473.
PRIDE	P15473.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	24484.
KEGG	rno:24484.
UCSC	RGD:2874. rat.
Organism-specific databases
CTD	3486.
RGD	2874. Igfbp3.
Phylogenomic databases
eggNOG	NOG38980.
HOGENOM	HOG000253012.
HOVERGEN	HBG002631.
InParanoid	P15473.
KO	K10138.
OrthoDB	EOG46144F.
Gene expression databases
ArrayExpress	P15473.
Genevestigator	P15473.
GermOnline	ENSRNOG00000008645. Rattus norvegicus.
Family and domain databases
Gene3D	4.10.800.10. 1 hit.
InterPro	IPR009030. Growth_fac_rcpt. IPR012211. IGFBP-3. IPR000867. IGFBP-like. IPR009168. IGFBP1-6. IPR022321. IGFBP_1-6_chordata. IPR017891. Insulin_GF-bd_Cys-rich_CS. IPR000716. Thyroglobulin_1. [Graphical view]
PANTHER	PTHR11551. PTHR11551. 1 hit. PTHR11551:SF3. PTHR11551:SF3. 1 hit.
Pfam	PF00219. IGFBP. 1 hit. PF00086. Thyroglobulin_1. 1 hit. [Graphical view]
PRINTS	PR01976. IGFBPFAMILY. PR01979. IGFBPFAMILY3.
SMART	SM00121. IB. 1 hit. SM00211. TY. 1 hit. [Graphical view]
SUPFAM	SSF57184. Grow_fac_recept. 1 hit. SSF57610. Thyroglobulin_1. 1 hit.
PROSITE	PS00222. IGFBP_N_1. 1 hit. PS51323. IGFBP_N_2. 1 hit. PS00484. THYROGLOBULIN_1_1. 1 hit. PS51162. THYROGLOBULIN_1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	603465.

Entry information

Entry name

IBP3_RAT

Accession

Primary (citable) accession number: P15473

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	August 1, 1990
Last modified:	April 3, 2013
This is version 118 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program