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Protein

Insulin-like growth factor-binding protein 3

Gene

Igfbp3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.

GO - Molecular functioni

  1. fibronectin binding Source: GO_Central
  2. insulin-like growth factor binding Source: RGD
  3. insulin-like growth factor I binding Source: RGD
  4. insulin-like growth factor II binding Source: RGD
  5. protein tyrosine phosphatase activator activity Source: UniProtKB

GO - Biological processi

  1. positive regulation of apoptotic process Source: UniProtKB
  2. positive regulation of catalytic activity Source: GOC
  3. positive regulation of myoblast differentiation Source: UniProtKB
  4. regulation of cell growth Source: InterPro
  5. regulation of insulin-like growth factor receptor signaling pathway Source: GO_Central
Complete GO annotation...

Keywords - Ligandi

Growth factor binding

Protein family/group databases

MEROPSiI31.952.

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin-like growth factor-binding protein 3
Short name:
IBP-3
Short name:
IGF-binding protein 3
Short name:
IGFBP-3
Gene namesi
Name:Igfbp3
Synonyms:Igfbp-3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi2874. Igfbp3.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: RGD
  2. insulin-like growth factor binary complex Source: RGD
  3. insulin-like growth factor ternary complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 27273 PublicationsAdd
BLAST
Chaini28 – 292265Insulin-like growth factor-binding protein 3PRO_0000014380Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi118 – 1181N-linked (GlcNAc...)Sequence Analysis
Glycosylationi124 – 1241N-linked (GlcNAc...)Sequence Analysis
Glycosylationi137 – 1371N-linked (GlcNAc...)Sequence Analysis
Glycosylationi200 – 2001N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi214 ↔ 241PROSITE-ProRule annotation
Disulfide bondi252 ↔ 263PROSITE-ProRule annotation
Disulfide bondi265 ↔ 286PROSITE-ProRule annotation

Post-translational modificationi

Phosphorylation sites are present in the extracellular medium.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP15473.
PRIDEiP15473.

PTM databases

PhosphoSiteiP15473.

Expressioni

Gene expression databases

GenevestigatoriP15473.

Interactioni

Subunit structurei

Interacts with XLKD1. Binds IGF2 more than IGF1. Forms a ternary complex of about 140 to 150 kDa with IGF1 or IGF2 and a 85 kDa glycoprotein (ALS). Interacts with HN. Interacts with TMEM219 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000011678.

Structurei

3D structure databases

ProteinModelPortaliP15473.
SMRiP15473. Positions 38-118.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini36 – 11984IGFBP N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini211 – 28676Thyroglobulin type-1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 IGFBP N-terminal domain.PROSITE-ProRule annotation
Contains 1 thyroglobulin type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG38980.
HOGENOMiHOG000253012.
HOVERGENiHBG002631.
InParanoidiP15473.
KOiK10138.
PhylomeDBiP15473.

Family and domain databases

Gene3Di4.10.40.20. 1 hit.
4.10.800.10. 1 hit.
InterProiIPR009030. Growth_fac_rcpt_N_dom.
IPR012211. IGFBP-3.
IPR000867. IGFBP-like.
IPR009168. IGFBP1-6.
IPR022321. IGFBP_1-6_chordata.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000716. Thyroglobulin_1.
[Graphical view]
PANTHERiPTHR11551. PTHR11551. 1 hit.
PTHR11551:SF3. PTHR11551:SF3. 1 hit.
PfamiPF00219. IGFBP. 1 hit.
PF00086. Thyroglobulin_1. 1 hit.
[Graphical view]
PRINTSiPR01976. IGFBPFAMILY.
PR01979. IGFBPFAMILY3.
SMARTiSM00121. IB. 1 hit.
SM00211. TY. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
SSF57610. SSF57610. 1 hit.
PROSITEiPS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS00484. THYROGLOBULIN_1_1. 1 hit.
PS51162. THYROGLOBULIN_1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15473-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MHPARPALWA AALTALTLLR GPPVARAGAG AVGAGPVVRC EPCDARALAQ
60 70 80 90 100
CAPPPTAPAC TELVREPGCG CCLTCALREG DACGVYTERC GTGLRCQPRP
110 120 130 140 150
AEQYPLKALL NGRGFCANAS AASNLSAYLP SQPSPGNTTE SEEDHNAGSV
160 170 180 190 200
ESQVVPSTHR VTDSKFHPLH SKMEVIIKGQ ARDSQRYKVD YESQSTDTQN
210 220 230 240 250
FSSESKRETE YGPCRREMED TLNHLKFLNV LSPRGVHIPN CDKKGFYKKK
260 270 280 290
QCRPSKGRKR GFCWCVDKYG QPLPGYDTKG KDDVHCLSVQ SQ
Length:292
Mass (Da):31,680
Last modified:August 1, 1990 - v2
Checksum:i1887D5E2A528F3B2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti8 – 81Missing in AAB00989. (PubMed:1689154)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31837 mRNA. Translation: AAA41383.1.
M33300 mRNA. Translation: AAB00989.1.
PIRiA36748.
RefSeqiNP_036720.2. NM_012588.2.
UniGeneiRn.26369.

Genome annotation databases

GeneIDi24484.
KEGGirno:24484.
UCSCiRGD:2874. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31837 mRNA. Translation: AAA41383.1.
M33300 mRNA. Translation: AAB00989.1.
PIRiA36748.
RefSeqiNP_036720.2. NM_012588.2.
UniGeneiRn.26369.

3D structure databases

ProteinModelPortaliP15473.
SMRiP15473. Positions 38-118.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000011678.

Protein family/group databases

MEROPSiI31.952.

PTM databases

PhosphoSiteiP15473.

Proteomic databases

PaxDbiP15473.
PRIDEiP15473.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24484.
KEGGirno:24484.
UCSCiRGD:2874. rat.

Organism-specific databases

CTDi3486.
RGDi2874. Igfbp3.

Phylogenomic databases

eggNOGiNOG38980.
HOGENOMiHOG000253012.
HOVERGENiHBG002631.
InParanoidiP15473.
KOiK10138.
PhylomeDBiP15473.

Miscellaneous databases

NextBioi603465.
PROiP15473.

Gene expression databases

GenevestigatoriP15473.

Family and domain databases

Gene3Di4.10.40.20. 1 hit.
4.10.800.10. 1 hit.
InterProiIPR009030. Growth_fac_rcpt_N_dom.
IPR012211. IGFBP-3.
IPR000867. IGFBP-like.
IPR009168. IGFBP1-6.
IPR022321. IGFBP_1-6_chordata.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000716. Thyroglobulin_1.
[Graphical view]
PANTHERiPTHR11551. PTHR11551. 1 hit.
PTHR11551:SF3. PTHR11551:SF3. 1 hit.
PfamiPF00219. IGFBP. 1 hit.
PF00086. Thyroglobulin_1. 1 hit.
[Graphical view]
PRINTSiPR01976. IGFBPFAMILY.
PR01979. IGFBPFAMILY3.
SMARTiSM00121. IB. 1 hit.
SM00211. TY. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
SSF57610. SSF57610. 1 hit.
PROSITEiPS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS00484. THYROGLOBULIN_1_1. 1 hit.
PS51162. THYROGLOBULIN_1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complementary DNA structure of the high molecular weight rat insulin-like growth factor binding protein (IGF-BP3) and tissue distribution of its mRNA."
    Shimasaki S., Koba A., Mercado M., Shimonaka M., Ling N.
    Biochem. Biophys. Res. Commun. 165:907-912(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning and characterization of the growth hormone-dependent insulin-like growth factor binding protein (IGFBP-3) in the rat."
    Albiston A.L., Herington A.C.
    Biochem. Biophys. Res. Commun. 166:892-897(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  3. "Isolation and NH2-terminal amino acid sequences of rat serum carrier proteins for insulin-like growth factors."
    Zapf J., Born W., Chang J.Y., James P., Froesch E.R., Fischer J.A.
    Biochem. Biophys. Res. Commun. 156:1187-1194(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 28-68.
  4. "Binding proteins for insulin-like growth factors in adult rat serum. Comparison with other human and rat binding proteins."
    Baxter R.C., Martin J.L.
    Biochem. Biophys. Res. Commun. 147:408-415(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 28-42.
  5. "Identification of a novel binding protein for insulin-like growth factors in adult rat serum."
    Shimonaka M., Schroeder R., Shimasaki S., Ling N.
    Biochem. Biophys. Res. Commun. 165:189-195(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 28-49.
    Tissue: Serum.

Entry informationi

Entry nameiIBP3_RAT
AccessioniPrimary (citable) accession number: P15473
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: August 1, 1990
Last modified: February 4, 2015
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.