Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P15473 (IBP3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Insulin-like growth factor-binding protein 3

Short name=IBP-3
Short name=IGF-binding protein 3
Short name=IGFBP-3
Gene names
Name:Igfbp3
Synonyms:Igfbp-3
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length292 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.

Subunit structure

Interacts with XLKD1. Binds IGF2 more than IGF1. Forms a ternary complex of about 140 to 150 kDa with IGF1 or IGF2 and a 85 kDa glycoprotein (ALS). Interacts with HN. Interacts with TMEM219 By similarity.

Subcellular location

Secreted.

Post-translational modification

Phosphorylation sites are present in the extracellular medium By similarity.

Sequence similarities

Contains 1 IGFBP N-terminal domain.

Contains 1 thyroglobulin type-1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Ref.3 Ref.4 Ref.5
Chain28 – 292265Insulin-like growth factor-binding protein 3
PRO_0000014380

Regions

Domain36 – 11984IGFBP N-terminal
Domain211 – 28676Thyroglobulin type-1

Amino acid modifications

Glycosylation1181N-linked (GlcNAc...) Potential
Glycosylation1241N-linked (GlcNAc...) Potential
Glycosylation1371N-linked (GlcNAc...) Potential
Glycosylation2001N-linked (GlcNAc...) Potential
Disulfide bond214 ↔ 241 By similarity
Disulfide bond252 ↔ 263 By similarity
Disulfide bond265 ↔ 286 By similarity

Experimental info

Sequence conflict81Missing in AAB00989. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P15473 [UniParc].

Last modified August 1, 1990. Version 2.
Checksum: 1887D5E2A528F3B2

FASTA29231,680
        10         20         30         40         50         60 
MHPARPALWA AALTALTLLR GPPVARAGAG AVGAGPVVRC EPCDARALAQ CAPPPTAPAC 

        70         80         90        100        110        120 
TELVREPGCG CCLTCALREG DACGVYTERC GTGLRCQPRP AEQYPLKALL NGRGFCANAS 

       130        140        150        160        170        180 
AASNLSAYLP SQPSPGNTTE SEEDHNAGSV ESQVVPSTHR VTDSKFHPLH SKMEVIIKGQ 

       190        200        210        220        230        240 
ARDSQRYKVD YESQSTDTQN FSSESKRETE YGPCRREMED TLNHLKFLNV LSPRGVHIPN 

       250        260        270        280        290 
CDKKGFYKKK QCRPSKGRKR GFCWCVDKYG QPLPGYDTKG KDDVHCLSVQ SQ 

« Hide

References

[1]"Complementary DNA structure of the high molecular weight rat insulin-like growth factor binding protein (IGF-BP3) and tissue distribution of its mRNA."
Shimasaki S., Koba A., Mercado M., Shimonaka M., Ling N.
Biochem. Biophys. Res. Commun. 165:907-912(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Cloning and characterization of the growth hormone-dependent insulin-like growth factor binding protein (IGFBP-3) in the rat."
Albiston A.L., Herington A.C.
Biochem. Biophys. Res. Commun. 166:892-897(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[3]"Isolation and NH2-terminal amino acid sequences of rat serum carrier proteins for insulin-like growth factors."
Zapf J., Born W., Chang J.Y., James P., Froesch E.R., Fischer J.A.
Biochem. Biophys. Res. Commun. 156:1187-1194(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 28-68.
[4]"Binding proteins for insulin-like growth factors in adult rat serum. Comparison with other human and rat binding proteins."
Baxter R.C., Martin J.L.
Biochem. Biophys. Res. Commun. 147:408-415(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 28-42.
[5]"Identification of a novel binding protein for insulin-like growth factors in adult rat serum."
Shimonaka M., Schroeder R., Shimasaki S., Ling N.
Biochem. Biophys. Res. Commun. 165:189-195(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 28-49.
Tissue: Serum.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M31837 mRNA. Translation: AAA41383.1.
M33300 mRNA. Translation: AAB00989.1.
PIRA36748.
RefSeqNP_036720.2. NM_012588.2.
UniGeneRn.26369.

3D structure databases

ProteinModelPortalP15473.
SMRP15473. Positions 38-118.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000011678.

Protein family/group databases

MEROPSI31.952.

PTM databases

PhosphoSiteP15473.

Proteomic databases

PaxDbP15473.
PRIDEP15473.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID24484.
KEGGrno:24484.
UCSCRGD:2874. rat.

Organism-specific databases

CTD3486.
RGD2874. Igfbp3.

Phylogenomic databases

eggNOGNOG38980.
HOGENOMHOG000253012.
HOVERGENHBG002631.
InParanoidP15473.
KOK10138.
PhylomeDBP15473.

Gene expression databases

GenevestigatorP15473.

Family and domain databases

Gene3D4.10.40.20. 1 hit.
4.10.800.10. 1 hit.
InterProIPR009030. Growth_fac_rcpt_N_dom.
IPR012211. IGFBP-3.
IPR000867. IGFBP-like.
IPR009168. IGFBP1-6.
IPR022321. IGFBP_1-6_chordata.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000716. Thyroglobulin_1.
[Graphical view]
PANTHERPTHR11551. PTHR11551. 1 hit.
PTHR11551:SF3. PTHR11551:SF3. 1 hit.
PfamPF00219. IGFBP. 1 hit.
PF00086. Thyroglobulin_1. 1 hit.
[Graphical view]
PRINTSPR01976. IGFBPFAMILY.
PR01979. IGFBPFAMILY3.
SMARTSM00121. IB. 1 hit.
SM00211. TY. 1 hit.
[Graphical view]
SUPFAMSSF57184. SSF57184. 1 hit.
SSF57610. SSF57610. 1 hit.
PROSITEPS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS00484. THYROGLOBULIN_1_1. 1 hit.
PS51162. THYROGLOBULIN_1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio603465.
PROP15473.

Entry information

Entry nameIBP3_RAT
AccessionPrimary (citable) accession number: P15473
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: August 1, 1990
Last modified: June 11, 2014
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families