Insulin-like growth factor-binding protein 3 precursor

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P15473 (IBP3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 108. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Insulin-like growth factor-binding protein 3
Short name=IBP-3
Short name=IGF-binding protein 3
Short name=IGFBP-3

Gene names

Name:	Igfbp3
Synonyms:	Igfbp-3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Subunit structure	Binds IGF2 more than IGF1. Forms a ternary complex of about 140 to 150 kDa with IGF1 or IGF2 and a 85 kDa glycoprotein (ALS). Interacts with XLKD1 By similarity.
Subcellular location	Secreted.
Post-translational modification	Phosphorylation sites are present in the extracelllular medium By similarity.
Sequence similarities	Contains 1 IGFBP N-terminal domain. Contains 1 thyroglobulin type-1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Growth factor binding
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	positive regulation of apoptotic process Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of myoblast differentiation Inferred from sequence or structural similarity. Source: UniProtKB regulation of cell growth Inferred from electronic annotation. Source: InterPro
Cellular component	insulin-like growth factor binary complex Inferred from direct assay. Source: RGD insulin-like growth factor ternary complex Inferred from direct assay. Source: RGD
Molecular function	insulin-like growth factor I binding Inferred from physical interaction. Source: RGD insulin-like growth factor II binding Inferred from physical interaction. Source: RGD protein tyrosine phosphatase activator activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 27

Ref.3 Ref.4 Ref.5

Chain

28 – 292

265

Insulin-like growth factor-binding protein 3

PRO_0000014380

Regions

Domain

36 – 119

IGFBP N-terminal

Domain

211 – 286

Thyroglobulin type-1

Amino acid modifications

Modified residue

196

Phosphothreonine By similarity

Modified residue

202

Phosphoserine By similarity

Glycosylation

118

N-linked (GlcNAc...) Potential

Glycosylation

124

N-linked (GlcNAc...) Potential

Glycosylation

137

N-linked (GlcNAc...) Potential

Glycosylation

200

N-linked (GlcNAc...) Potential

Disulfide bond

214 ↔ 241

By similarity

Disulfide bond

252 ↔ 263

By similarity

Disulfide bond

265 ↔ 286

By similarity

Experimental info

Sequence conflict

Missing in AAB00989. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P15473 [UniParc].

Last modified August 1, 1990. Version 2.
Checksum: 1887D5E2A528F3B2
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FASTA

292

31,680

        10         20         30         40         50         60 
MHPARPALWA AALTALTLLR GPPVARAGAG AVGAGPVVRC EPCDARALAQ CAPPPTAPAC 

        70         80         90        100        110        120 
TELVREPGCG CCLTCALREG DACGVYTERC GTGLRCQPRP AEQYPLKALL NGRGFCANAS 

       130        140        150        160        170        180 
AASNLSAYLP SQPSPGNTTE SEEDHNAGSV ESQVVPSTHR VTDSKFHPLH SKMEVIIKGQ 

       190        200        210        220        230        240 
ARDSQRYKVD YESQSTDTQN FSSESKRETE YGPCRREMED TLNHLKFLNV LSPRGVHIPN 

       250        260        270        280        290 
CDKKGFYKKK QCRPSKGRKR GFCWCVDKYG QPLPGYDTKG KDDVHCLSVQ SQ

« Hide

References

[1]	"Complementary DNA structure of the high molecular weight rat insulin-like growth factor binding protein (IGF-BP3) and tissue distribution of its mRNA." Shimasaki S., Koba A., Mercado M., Shimonaka M., Ling N. Biochem. Biophys. Res. Commun. 165:907-912(1989) [PubMed: 2480787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning and characterization of the growth hormone-dependent insulin-like growth factor binding protein (IGFBP-3) in the rat." Albiston A.L., Herington A.C. Biochem. Biophys. Res. Commun. 166:892-897(1990) [PubMed: 1689154] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[3]	"Isolation and NH2-terminal amino acid sequences of rat serum carrier proteins for insulin-like growth factors." Zapf J., Born W., Chang J.Y., James P., Froesch E.R., Fischer J.A. Biochem. Biophys. Res. Commun. 156:1187-1194(1988) [PubMed: 3190697] [Abstract] Cited for: PROTEIN SEQUENCE OF 28-68.
[4]	"Binding proteins for insulin-like growth factors in adult rat serum. Comparison with other human and rat binding proteins." Baxter R.C., Martin J.L. Biochem. Biophys. Res. Commun. 147:408-415(1987) [PubMed: 2443135] [Abstract] Cited for: PROTEIN SEQUENCE OF 28-42.
[5]	"Identification of a novel binding protein for insulin-like growth factors in adult rat serum." Shimonaka M., Schroeder R., Shimasaki S., Ling N. Biochem. Biophys. Res. Commun. 165:189-195(1989) [PubMed: 2480123] [Abstract] Cited for: PROTEIN SEQUENCE OF 28-49. Tissue: Serum.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M31837 mRNA. Translation: AAA41383.1. M33300 mRNA. Translation: AAB00989.1.
IPI	IPI00209369.
PIR	A36748.
RefSeq	NP_036720.2. NM_012588.2.
UniGene	Rn.26369.
3D structure databases
ProteinModelPortal	P15473.
SMR	P15473. Positions 38-118.
ModBase	Search...
Protein-protein interaction databases
STRING	P15473.
Protein family/group databases
MEROPS	I31.952.
PTM databases
PhosphoSite	P15473.
Proteomic databases
PRIDE	P15473.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	24484.
KEGG	rno:24484.
Organism-specific databases
CTD	3486.
RGD	2874. Igfbp3.
Phylogenomic databases
eggNOG	roNOG09896.
GeneTree	ENSGT00550000074457.
HOVERGEN	HBG002631.
InParanoid	P15473.
OrthoDB	EOG46144F.
Gene expression databases
ArrayExpress	P15473.
Genevestigator	P15473.
GermOnline	ENSRNOG00000008645. Rattus norvegicus.
Family and domain databases
InterPro	IPR009030. Growth_fac_rcpt. IPR012211. IGFBP-3. IPR000867. IGFBP-like. IPR009168. IGFBP1-6. IPR022321. IGFBP_1-6_chordata. IPR017891. Insulin_GF-bd_Cys-rich_CS. IPR000716. Thyroglobulin_1. [Graphical view]
Gene3D	G3DSA:4.10.800.10. Thyroglobulin_1. 1 hit.
KO	K10138.
PANTHER	PTHR11551:SF3. IGFBP-3. 1 hit. PTHR11551. IGFBP1-6. 1 hit.
Pfam	PF00219. IGFBP. 1 hit. PF00086. Thyroglobulin_1. 1 hit. [Graphical view]
PRINTS	PR01976. IGFBPFAMILY. PR01979. IGFBPFAMILY3.
SMART	SM00121. IB. 1 hit. SM00211. TY. 1 hit. [Graphical view]
SUPFAM	SSF57184. Grow_fac_recept. 1 hit. SSF57610. Thyroglobulin_1. 1 hit.
PROSITE	PS00222. IGFBP_N_1. 1 hit. PS51323. IGFBP_N_2. 1 hit. PS00484. THYROGLOBULIN_1_1. 1 hit. PS51162. THYROGLOBULIN_1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	603465.

Entry information

Entry name

IBP3_RAT

Accession

Primary (citable) accession number: P15473

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	August 1, 1990
Last modified:	November 16, 2011
This is version 108 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents