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Protein

Insulin-like growth factor-binding protein 3

Gene

Igfbp3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.

GO - Molecular functioni

  • fibronectin binding Source: GO_Central
  • insulin-like growth factor I binding Source: RGD
  • insulin-like growth factor II binding Source: RGD
  • protein tyrosine phosphatase activator activity Source: UniProtKB

GO - Biological processi

  • aging Source: RGD
  • negative regulation of cell proliferation Source: RGD
  • positive regulation of apoptotic DNA fragmentation Source: RGD
  • positive regulation of apoptotic process Source: UniProtKB
  • positive regulation of cardiac muscle cell apoptotic process Source: RGD
  • positive regulation of myoblast differentiation Source: UniProtKB
  • regulation of cell growth Source: InterPro
  • regulation of insulin-like growth factor receptor signaling pathway Source: GO_Central
Complete GO annotation...

Keywords - Ligandi

Growth factor binding

Protein family/group databases

MEROPSiI31.952.

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin-like growth factor-binding protein 3
Short name:
IBP-3
Short name:
IGF-binding protein 3
Short name:
IGFBP-3
Gene namesi
Name:Igfbp3
Synonyms:Igfbp-3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2874. Igfbp3.

Subcellular locationi

GO - Cellular componenti

  • extracellular space Source: RGD
  • insulin-like growth factor binary complex Source: RGD
  • insulin-like growth factor ternary complex Source: RGD
  • nuclear heterochromatin Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 273 PublicationsAdd BLAST27
ChainiPRO_000001438028 – 292Insulin-like growth factor-binding protein 3Add BLAST265

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi118N-linked (GlcNAc...)Sequence analysis1
Glycosylationi124N-linked (GlcNAc...)Sequence analysis1
Glycosylationi137N-linked (GlcNAc...)Sequence analysis1
Modified residuei149PhosphoserineBy similarity1
Glycosylationi200N-linked (GlcNAc...)Sequence analysis1
Modified residuei202PhosphoserineBy similarity1
Disulfide bondi214 ↔ 241PROSITE-ProRule annotation
Disulfide bondi252 ↔ 263PROSITE-ProRule annotation
Disulfide bondi265 ↔ 286PROSITE-ProRule annotation

Post-translational modificationi

Phosphorylated by FAM20C in the extracellular medium.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP15473.
PRIDEiP15473.

PTM databases

PhosphoSitePlusiP15473.

Interactioni

Subunit structurei

Interacts with XLKD1. Binds IGF2 more than IGF1. Forms a ternary complex of about 140 to 150 kDa with IGF1 or IGF2 and a 85 kDa glycoprotein (ALS). Interacts with HN. Interacts with TMEM219 (By similarity).By similarity

GO - Molecular functioni

  • fibronectin binding Source: GO_Central
  • insulin-like growth factor I binding Source: RGD
  • insulin-like growth factor II binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000011678.

Structurei

3D structure databases

ProteinModelPortaliP15473.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 119IGFBP N-terminalPROSITE-ProRule annotationAdd BLAST84
Domaini211 – 286Thyroglobulin type-1PROSITE-ProRule annotationAdd BLAST76

Sequence similaritiesi

Contains 1 IGFBP N-terminal domain.PROSITE-ProRule annotation
Contains 1 thyroglobulin type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IHZU. Eukaryota.
ENOG4111MF3. LUCA.
HOGENOMiHOG000253012.
HOVERGENiHBG002631.
InParanoidiP15473.
KOiK10138.
PhylomeDBiP15473.

Family and domain databases

Gene3Di4.10.800.10. 1 hit.
InterProiIPR009030. Growth_fac_rcpt_.
IPR012211. IGFBP-3.
IPR000867. IGFBP-like.
IPR009168. IGFBP1-6.
IPR022321. IGFBP_1-6_chordata.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000716. Thyroglobulin_1.
[Graphical view]
PANTHERiPTHR11551. PTHR11551. 1 hit.
PTHR11551:SF3. PTHR11551:SF3. 1 hit.
PfamiPF00219. IGFBP. 1 hit.
PF00086. Thyroglobulin_1. 1 hit.
[Graphical view]
PRINTSiPR01976. IGFBPFAMILY.
PR01979. IGFBPFAMILY3.
SMARTiSM00121. IB. 1 hit.
SM00211. TY. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
SSF57610. SSF57610. 1 hit.
PROSITEiPS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS00484. THYROGLOBULIN_1_1. 1 hit.
PS51162. THYROGLOBULIN_1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15473-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHPARPALWA AALTALTLLR GPPVARAGAG AVGAGPVVRC EPCDARALAQ
60 70 80 90 100
CAPPPTAPAC TELVREPGCG CCLTCALREG DACGVYTERC GTGLRCQPRP
110 120 130 140 150
AEQYPLKALL NGRGFCANAS AASNLSAYLP SQPSPGNTTE SEEDHNAGSV
160 170 180 190 200
ESQVVPSTHR VTDSKFHPLH SKMEVIIKGQ ARDSQRYKVD YESQSTDTQN
210 220 230 240 250
FSSESKRETE YGPCRREMED TLNHLKFLNV LSPRGVHIPN CDKKGFYKKK
260 270 280 290
QCRPSKGRKR GFCWCVDKYG QPLPGYDTKG KDDVHCLSVQ SQ
Length:292
Mass (Da):31,680
Last modified:August 1, 1990 - v2
Checksum:i1887D5E2A528F3B2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8Missing in AAB00989 (PubMed:1689154).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31837 mRNA. Translation: AAA41383.1.
M33300 mRNA. Translation: AAB00989.1.
PIRiA36748.
RefSeqiNP_036720.2. NM_012588.2.
UniGeneiRn.26369.

Genome annotation databases

GeneIDi24484.
KEGGirno:24484.
UCSCiRGD:2874. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31837 mRNA. Translation: AAA41383.1.
M33300 mRNA. Translation: AAB00989.1.
PIRiA36748.
RefSeqiNP_036720.2. NM_012588.2.
UniGeneiRn.26369.

3D structure databases

ProteinModelPortaliP15473.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000011678.

Protein family/group databases

MEROPSiI31.952.

PTM databases

PhosphoSitePlusiP15473.

Proteomic databases

PaxDbiP15473.
PRIDEiP15473.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24484.
KEGGirno:24484.
UCSCiRGD:2874. rat.

Organism-specific databases

CTDi3486.
RGDi2874. Igfbp3.

Phylogenomic databases

eggNOGiENOG410IHZU. Eukaryota.
ENOG4111MF3. LUCA.
HOGENOMiHOG000253012.
HOVERGENiHBG002631.
InParanoidiP15473.
KOiK10138.
PhylomeDBiP15473.

Miscellaneous databases

PROiP15473.

Family and domain databases

Gene3Di4.10.800.10. 1 hit.
InterProiIPR009030. Growth_fac_rcpt_.
IPR012211. IGFBP-3.
IPR000867. IGFBP-like.
IPR009168. IGFBP1-6.
IPR022321. IGFBP_1-6_chordata.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000716. Thyroglobulin_1.
[Graphical view]
PANTHERiPTHR11551. PTHR11551. 1 hit.
PTHR11551:SF3. PTHR11551:SF3. 1 hit.
PfamiPF00219. IGFBP. 1 hit.
PF00086. Thyroglobulin_1. 1 hit.
[Graphical view]
PRINTSiPR01976. IGFBPFAMILY.
PR01979. IGFBPFAMILY3.
SMARTiSM00121. IB. 1 hit.
SM00211. TY. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
SSF57610. SSF57610. 1 hit.
PROSITEiPS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS00484. THYROGLOBULIN_1_1. 1 hit.
PS51162. THYROGLOBULIN_1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIBP3_RAT
AccessioniPrimary (citable) accession number: P15473
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: August 1, 1990
Last modified: November 2, 2016
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.