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P15473

- IBP3_RAT

UniProt

P15473 - IBP3_RAT

Protein

Insulin-like growth factor-binding protein 3

Gene

Igfbp3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 2 (01 Aug 1990)
      Previous versions | rss
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    Functioni

    IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.

    GO - Molecular functioni

    1. insulin-like growth factor binding Source: RGD
    2. insulin-like growth factor I binding Source: RGD
    3. insulin-like growth factor II binding Source: RGD
    4. protein binding Source: RGD
    5. protein tyrosine phosphatase activator activity Source: UniProtKB

    GO - Biological processi

    1. positive regulation of apoptotic process Source: UniProtKB
    2. positive regulation of catalytic activity Source: GOC
    3. positive regulation of myoblast differentiation Source: UniProtKB
    4. regulation of cell growth Source: InterPro

    Keywords - Ligandi

    Growth factor binding

    Protein family/group databases

    MEROPSiI31.952.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Insulin-like growth factor-binding protein 3
    Short name:
    IBP-3
    Short name:
    IGF-binding protein 3
    Short name:
    IGFBP-3
    Gene namesi
    Name:Igfbp3
    Synonyms:Igfbp-3
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi2874. Igfbp3.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: RGD
    2. insulin-like growth factor binary complex Source: RGD
    3. insulin-like growth factor ternary complex Source: RGD

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 27273 PublicationsAdd
    BLAST
    Chaini28 – 292265Insulin-like growth factor-binding protein 3PRO_0000014380Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi118 – 1181N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi124 – 1241N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi137 – 1371N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi200 – 2001N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi214 ↔ 241PROSITE-ProRule annotation
    Disulfide bondi252 ↔ 263PROSITE-ProRule annotation
    Disulfide bondi265 ↔ 286PROSITE-ProRule annotation

    Post-translational modificationi

    Phosphorylation sites are present in the extracellular medium.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiP15473.
    PRIDEiP15473.

    PTM databases

    PhosphoSiteiP15473.

    Expressioni

    Gene expression databases

    GenevestigatoriP15473.

    Interactioni

    Subunit structurei

    Interacts with XLKD1. Binds IGF2 more than IGF1. Forms a ternary complex of about 140 to 150 kDa with IGF1 or IGF2 and a 85 kDa glycoprotein (ALS). Interacts with HN. Interacts with TMEM219 By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000011678.

    Structurei

    3D structure databases

    ProteinModelPortaliP15473.
    SMRiP15473. Positions 38-118.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini36 – 11984IGFBP N-terminalPROSITE-ProRule annotationAdd
    BLAST
    Domaini211 – 28676Thyroglobulin type-1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 IGFBP N-terminal domain.PROSITE-ProRule annotation
    Contains 1 thyroglobulin type-1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG38980.
    HOGENOMiHOG000253012.
    HOVERGENiHBG002631.
    InParanoidiP15473.
    KOiK10138.
    PhylomeDBiP15473.

    Family and domain databases

    Gene3Di4.10.40.20. 1 hit.
    4.10.800.10. 1 hit.
    InterProiIPR009030. Growth_fac_rcpt_N_dom.
    IPR012211. IGFBP-3.
    IPR000867. IGFBP-like.
    IPR009168. IGFBP1-6.
    IPR022321. IGFBP_1-6_chordata.
    IPR017891. Insulin_GF-bd_Cys-rich_CS.
    IPR000716. Thyroglobulin_1.
    [Graphical view]
    PANTHERiPTHR11551. PTHR11551. 1 hit.
    PTHR11551:SF3. PTHR11551:SF3. 1 hit.
    PfamiPF00219. IGFBP. 1 hit.
    PF00086. Thyroglobulin_1. 1 hit.
    [Graphical view]
    PRINTSiPR01976. IGFBPFAMILY.
    PR01979. IGFBPFAMILY3.
    SMARTiSM00121. IB. 1 hit.
    SM00211. TY. 1 hit.
    [Graphical view]
    SUPFAMiSSF57184. SSF57184. 1 hit.
    SSF57610. SSF57610. 1 hit.
    PROSITEiPS00222. IGFBP_N_1. 1 hit.
    PS51323. IGFBP_N_2. 1 hit.
    PS00484. THYROGLOBULIN_1_1. 1 hit.
    PS51162. THYROGLOBULIN_1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P15473-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHPARPALWA AALTALTLLR GPPVARAGAG AVGAGPVVRC EPCDARALAQ    50
    CAPPPTAPAC TELVREPGCG CCLTCALREG DACGVYTERC GTGLRCQPRP 100
    AEQYPLKALL NGRGFCANAS AASNLSAYLP SQPSPGNTTE SEEDHNAGSV 150
    ESQVVPSTHR VTDSKFHPLH SKMEVIIKGQ ARDSQRYKVD YESQSTDTQN 200
    FSSESKRETE YGPCRREMED TLNHLKFLNV LSPRGVHIPN CDKKGFYKKK 250
    QCRPSKGRKR GFCWCVDKYG QPLPGYDTKG KDDVHCLSVQ SQ 292
    Length:292
    Mass (Da):31,680
    Last modified:August 1, 1990 - v2
    Checksum:i1887D5E2A528F3B2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti8 – 81Missing in AAB00989. (PubMed:1689154)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M31837 mRNA. Translation: AAA41383.1.
    M33300 mRNA. Translation: AAB00989.1.
    PIRiA36748.
    RefSeqiNP_036720.2. NM_012588.2.
    UniGeneiRn.26369.

    Genome annotation databases

    GeneIDi24484.
    KEGGirno:24484.
    UCSCiRGD:2874. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M31837 mRNA. Translation: AAA41383.1 .
    M33300 mRNA. Translation: AAB00989.1 .
    PIRi A36748.
    RefSeqi NP_036720.2. NM_012588.2.
    UniGenei Rn.26369.

    3D structure databases

    ProteinModelPortali P15473.
    SMRi P15473. Positions 38-118.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000011678.

    Protein family/group databases

    MEROPSi I31.952.

    PTM databases

    PhosphoSitei P15473.

    Proteomic databases

    PaxDbi P15473.
    PRIDEi P15473.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 24484.
    KEGGi rno:24484.
    UCSCi RGD:2874. rat.

    Organism-specific databases

    CTDi 3486.
    RGDi 2874. Igfbp3.

    Phylogenomic databases

    eggNOGi NOG38980.
    HOGENOMi HOG000253012.
    HOVERGENi HBG002631.
    InParanoidi P15473.
    KOi K10138.
    PhylomeDBi P15473.

    Miscellaneous databases

    NextBioi 603465.
    PROi P15473.

    Gene expression databases

    Genevestigatori P15473.

    Family and domain databases

    Gene3Di 4.10.40.20. 1 hit.
    4.10.800.10. 1 hit.
    InterProi IPR009030. Growth_fac_rcpt_N_dom.
    IPR012211. IGFBP-3.
    IPR000867. IGFBP-like.
    IPR009168. IGFBP1-6.
    IPR022321. IGFBP_1-6_chordata.
    IPR017891. Insulin_GF-bd_Cys-rich_CS.
    IPR000716. Thyroglobulin_1.
    [Graphical view ]
    PANTHERi PTHR11551. PTHR11551. 1 hit.
    PTHR11551:SF3. PTHR11551:SF3. 1 hit.
    Pfami PF00219. IGFBP. 1 hit.
    PF00086. Thyroglobulin_1. 1 hit.
    [Graphical view ]
    PRINTSi PR01976. IGFBPFAMILY.
    PR01979. IGFBPFAMILY3.
    SMARTi SM00121. IB. 1 hit.
    SM00211. TY. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57184. SSF57184. 1 hit.
    SSF57610. SSF57610. 1 hit.
    PROSITEi PS00222. IGFBP_N_1. 1 hit.
    PS51323. IGFBP_N_2. 1 hit.
    PS00484. THYROGLOBULIN_1_1. 1 hit.
    PS51162. THYROGLOBULIN_1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complementary DNA structure of the high molecular weight rat insulin-like growth factor binding protein (IGF-BP3) and tissue distribution of its mRNA."
      Shimasaki S., Koba A., Mercado M., Shimonaka M., Ling N.
      Biochem. Biophys. Res. Commun. 165:907-912(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Cloning and characterization of the growth hormone-dependent insulin-like growth factor binding protein (IGFBP-3) in the rat."
      Albiston A.L., Herington A.C.
      Biochem. Biophys. Res. Commun. 166:892-897(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    3. "Isolation and NH2-terminal amino acid sequences of rat serum carrier proteins for insulin-like growth factors."
      Zapf J., Born W., Chang J.Y., James P., Froesch E.R., Fischer J.A.
      Biochem. Biophys. Res. Commun. 156:1187-1194(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 28-68.
    4. "Binding proteins for insulin-like growth factors in adult rat serum. Comparison with other human and rat binding proteins."
      Baxter R.C., Martin J.L.
      Biochem. Biophys. Res. Commun. 147:408-415(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 28-42.
    5. "Identification of a novel binding protein for insulin-like growth factors in adult rat serum."
      Shimonaka M., Schroeder R., Shimasaki S., Ling N.
      Biochem. Biophys. Res. Commun. 165:189-195(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 28-49.
      Tissue: Serum.

    Entry informationi

    Entry nameiIBP3_RAT
    AccessioniPrimary (citable) accession number: P15473
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: August 1, 1990
    Last modified: October 1, 2014
    This is version 128 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3