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Protein

12S seed storage protein CRB

Gene

CRB

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Seed storage protein.

GO - Molecular functioni

  • nutrient reservoir activity Source: UniProtKB

GO - Biological processi

  • cellular response to abscisic acid stimulus Source: UniProtKB
  • response to abscisic acid Source: TAIR
  • seed maturation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Seed storage protein, Storage protein

Names & Taxonomyi

Protein namesi
Recommended name:
12S seed storage protein CRB
Alternative name(s):
Cruciferin 2
Short name:
AtCRU2
Cruciferin B
Legumin-type globulin storage protein CRU2
Cleaved into the following 2 chains:
Alternative name(s):
12S seed storage protein CRB acidic chain
Alternative name(s):
12S seed storage protein CRB basic chain
Gene namesi
Name:CRB
Synonyms:CRU2, CRU3
Ordered Locus Names:At1g03880
ORF Names:F21M11.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G03880.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24By similarityAdd BLAST24
ChainiPRO_000003200125 – 26912S seed storage protein CRB alpha chainBy similarityAdd BLAST245
ChainiPRO_0000032002270 – 45512S seed storage protein CRB beta chainBy similarityAdd BLAST186

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi30 ↔ 63By similarity
Disulfide bondi106 ↔ 276Interchain (between alpha and beta chains)Sequence analysis
Modified residuei109Phosphothreonine1 Publication1
Modified residuei299Phosphotyrosine1 Publication1
Modified residuei301PhosphoserineBy similarity1
Modified residuei367Phosphoserine1 Publication1
Modified residuei395Phosphothreonine1 Publication1
Modified residuei420Phosphothreonine1 Publication1
Modified residuei436Phosphoserine1 Publication1

Post-translational modificationi

Ubiquitinated.By similarity
Proteolytically processed during seed maturation at a conserved Asn-Gly peptide bond by an asparaginyl endopeptidase to produce two mature polypeptides referred to as alpha and beta subunits that are joined together by a disulfide bond.
Phosphorylated in seeds on some Tyr residues in response to abscisic acid (ABA).2 Publications

Keywords - PTMi

Disulfide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP15456.
PRIDEiP15456.

PTM databases

iPTMnetiP15456.

Expressioni

Tissue specificityi

Accumulates in seeds 8 days after anthesis.2 Publications

Developmental stagei

Detected in siliques at nucleotide level from 6 days post anthesis (dpa) to 17 dpa. First observed in siliques at protein level 12 dpa and accumulates progressively as native isoforms or proteolytic fragments during the last week of seed maturation/desiccation. Present in dry seeds, but disappears during their germination (at protein level).2 Publications

Gene expression databases

GenevisibleiP15456. AT.

Interactioni

Subunit structurei

Hexamer; each subunit is composed of an acidic and a basic chain derived from a single precursor and linked by a disulfide bond.

Protein-protein interaction databases

STRINGi3702.AT1G03880.1.

Structurei

3D structure databases

ProteinModelPortaliP15456.
SMRiP15456.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IHSE. Eukaryota.
ENOG410YATH. LUCA.
HOGENOMiHOG000217279.
InParanoidiP15456.
OMAiWNINAHA.
OrthoDBiEOG093607KS.
PhylomeDBiP15456.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
InterProiIPR022379. 11S_seedstore_CS.
IPR006044. 11S_seedstore_pln.
IPR006045. Cupin_1.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF00190. Cupin_1. 2 hits.
[Graphical view]
PRINTSiPR00439. 11SGLOBULIN.
SMARTiSM00835. Cupin_1. 2 hits.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
PROSITEiPS00305. 11S_SEED_STORAGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15456-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRVSSIISF SLTLLILFNG YTAQQWPNEC QLDQLNALEP SQIIKSEGGR
60 70 80 90 100
IEVWDHHAPQ LRCSGFAFER FVIEPQGLFL PTFLNAGKLT FVVHGRGLMG
110 120 130 140 150
RVIPGCAETF MESPVFGEGQ GQGQSQGFRD MHQKVEHLRC GDTIATPSGV
160 170 180 190 200
AQWFYNNGNE PLILVAAADL ASNQNQLDRN LRPFLIAGNN PQGQEWLQGR
210 220 230 240 250
KQQKQNNIFN GFAPEILAQA FKINVETAQQ LQNQQDNRGN IVKVNGPFGV
260 270 280 290 300
IRPPLRRGEG GQQPHEIANG LEETLCTMRC TENLDDPSDA DVYKPSLGYI
310 320 330 340 350
STLNSYNLPI LRLLRLSALR GSIRKNAMVL PQWNVNANAA LYVTNGKAHI
360 370 380 390 400
QMVNDNGERV FDQEISSGQL LVVPQGFSVM KHAIGEQFEW IEFKTNENAQ
410 420 430 440 450
VNTLAGRTSV MRGLPLEVIT NGYQISPEEA KRVKFSTIET TLTHSSPMSY

GRPRA
Length:455
Mass (Da):50,558
Last modified:June 20, 2002 - v2
Checksum:iBE24BCBD2F69B538
GO

Sequence cautioni

The sequence BAD94859 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti383A → R in AAA32778 (Ref. 1) Curated1
Sequence conflicti383A → R in CAA32494 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M37248 Genomic DNA. Translation: AAA32778.1.
X14313 Genomic DNA. Translation: CAA32494.1.
AC003027 Genomic DNA. Translation: AAD10680.1.
CP002684 Genomic DNA. Translation: AEE27628.1.
AY093005 mRNA. Translation: AAM13004.1.
BT009682 mRNA. Translation: AAP81800.1.
Z17654 mRNA. Translation: CAA79024.1.
AK222062 mRNA. Translation: BAD94859.1. Different initiation.
PIRiE86169.
S08510.
RefSeqiNP_171884.1. NM_100268.5.
UniGeneiAt.20.
At.71940.

Genome annotation databases

EnsemblPlantsiAT1G03880.1; AT1G03880.1; AT1G03880.
GeneIDi839383.
GrameneiAT1G03880.1; AT1G03880.1; AT1G03880.
KEGGiath:AT1G03880.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M37248 Genomic DNA. Translation: AAA32778.1.
X14313 Genomic DNA. Translation: CAA32494.1.
AC003027 Genomic DNA. Translation: AAD10680.1.
CP002684 Genomic DNA. Translation: AEE27628.1.
AY093005 mRNA. Translation: AAM13004.1.
BT009682 mRNA. Translation: AAP81800.1.
Z17654 mRNA. Translation: CAA79024.1.
AK222062 mRNA. Translation: BAD94859.1. Different initiation.
PIRiE86169.
S08510.
RefSeqiNP_171884.1. NM_100268.5.
UniGeneiAt.20.
At.71940.

3D structure databases

ProteinModelPortaliP15456.
SMRiP15456.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G03880.1.

PTM databases

iPTMnetiP15456.

Proteomic databases

PaxDbiP15456.
PRIDEiP15456.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G03880.1; AT1G03880.1; AT1G03880.
GeneIDi839383.
GrameneiAT1G03880.1; AT1G03880.1; AT1G03880.
KEGGiath:AT1G03880.

Organism-specific databases

TAIRiAT1G03880.

Phylogenomic databases

eggNOGiENOG410IHSE. Eukaryota.
ENOG410YATH. LUCA.
HOGENOMiHOG000217279.
InParanoidiP15456.
OMAiWNINAHA.
OrthoDBiEOG093607KS.
PhylomeDBiP15456.

Miscellaneous databases

PROiP15456.

Gene expression databases

GenevisibleiP15456. AT.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
InterProiIPR022379. 11S_seedstore_CS.
IPR006044. 11S_seedstore_pln.
IPR006045. Cupin_1.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF00190. Cupin_1. 2 hits.
[Graphical view]
PRINTSiPR00439. 11SGLOBULIN.
SMARTiSM00835. Cupin_1. 2 hits.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
PROSITEiPS00305. 11S_SEED_STORAGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCRU2_ARATH
AccessioniPrimary (citable) accession number: P15456
Secondary accession number(s): Q56WH8, Q9SAW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: June 20, 2002
Last modified: November 2, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.