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Protein

12S seed storage protein CRA1

Gene

CRA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Seed storage protein.

GO - Molecular functioni

  • nutrient reservoir activity Source: UniProtKB

GO - Biological processi

  • cellular response to abscisic acid stimulus Source: UniProtKB
  • response to abscisic acid Source: TAIR
  • seed maturation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Seed storage protein, Storage protein

Names & Taxonomyi

Protein namesi
Recommended name:
12S seed storage protein CRA1
Alternative name(s):
Cruciferin 1
Short name:
AtCRU1
Cruciferin A1
Legumin-type globulin storage protein CRA1
Cleaved into the following 2 chains:
Alternative name(s):
12S seed storage protein CRA1 acidic chain
Alternative name(s):
12S seed storage protein CRA1 basic chain
Gene namesi
Name:CRA1
Synonyms:CRU1, CRU4
Ordered Locus Names:At5g44120
ORF Names:MLN1.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G44120.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424By similarityAdd
BLAST
Chaini25 – 28225812S seed storage protein CRA1 alpha chainBy similarityPRO_0000031999Add
BLAST
Chaini283 – 47219012S seed storage protein CRA1 beta chainBy similarityPRO_0000032000Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi36 ↔ 69By similarity
Disulfide bondi112 ↔ 289Interchain (between alpha and beta chains)Sequence analysis
Modified residuei115 – 1151Phosphothreonine1 Publication
Modified residuei312 – 3121Phosphotyrosine1 Publication
Modified residuei314 – 3141Phosphoserine1 Publication
Modified residuei408 – 4081PhosphothreonineBy similarity
Modified residuei433 – 4331PhosphothreonineBy similarity

Post-translational modificationi

Ubiquitinated.1 Publication
Phosphorylated in seeds on some Tyr residues in response to abscisic acid (ABA).2 Publications
Proteolytically processed during seed maturation at a conserved Asn-Gly peptide bond by an asparaginyl endopeptidase to produce two mature polypeptides referred to as alpha and beta subunits that are joined together by a disulfide bond.

Keywords - PTMi

Disulfide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP15455.
PRIDEiP15455.

PTM databases

iPTMnetiP15455.

Expressioni

Tissue specificityi

Accumulates in seeds 8 days after anthesis.2 Publications

Developmental stagei

Detected in siliques at nucleotide level from 6 days post anthesis (dpa) to 17 dpa. First observed in siliques at protein level 15 dpa and accumulates progressively as native isoforms or proteolytic fragments during the last week of seed maturation/desiccation. Present in dry seeds, essentially in cotyledons and hypocotyls, but disappears during their germination (at protein level).2 Publications

Gene expression databases

ExpressionAtlasiP15455. baseline and differential.
GenevisibleiP15455. AT.

Interactioni

Subunit structurei

Hexamer; each subunit is composed of an acidic and a basic chain derived from a single precursor and linked by a disulfide bond.

Protein-protein interaction databases

BioGridi19685. 3 interactions.
STRINGi3702.AT5G44120.3.

Structurei

3D structure databases

ProteinModelPortaliP15455.
SMRiP15455. Positions 30-459.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IHSE. Eukaryota.
ENOG410YATH. LUCA.
HOGENOMiHOG000217279.
InParanoidiP15455.
OMAiHENAMIN.
OrthoDBiEOG093607KS.
PhylomeDBiP15455.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
InterProiIPR022379. 11S_seedstore_CS.
IPR006044. 11S_seedstore_pln.
IPR006045. Cupin_1.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF00190. Cupin_1. 2 hits.
[Graphical view]
PRINTSiPR00439. 11SGLOBULIN.
SMARTiSM00835. Cupin_1. 2 hits.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 2 hits.
PROSITEiPS00305. 11S_SEED_STORAGE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P15455-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARVSSLLSF CLTLLILFHG YAAQQGQQGQ QFPNECQLDQ LNALEPSHVL
60 70 80 90 100
KSEAGRIEVW DHHAPQLRCS GVSFARYIIE SKGLYLPSFF NTAKLSFVAK
110 120 130 140 150
GRGLMGKVIP GCAETFQDSS EFQPRFEGQG QSQRFRDMHQ KVEHIRSGDT
160 170 180 190 200
IATTPGVAQW FYNDGQEPLV IVSVFDLASH QNQLDRNPRP FYLAGNNPQG
210 220 230 240 250
QVWLQGREQQ PQKNIFNGFG PEVIAQALKI DLQTAQQLQN QDDNRGNIVR
260 270 280 290 300
VQGPFGVIRP PLRGQRPQEE EEEEGRHGRH GNGLEETICS ARCTDNLDDP
310 320 330 340 350
SRADVYKPQL GYISTLNSYD LPILRFIRLS ALRGSIRQNA MVLPQWNANA
360 370 380 390 400
NAILYVTDGE AQIQIVNDNG NRVFDGQVSQ GQLIAVPQGF SVVKRATSNR
410 420 430 440 450
FQWVEFKTNA NAQINTLAGR TSVLRGLPLE VITNGFQISP EEARRVKFNT
460 470
LETTLTHSSG PASYGRPRVA AA
Length:472
Mass (Da):52,595
Last modified:June 20, 2002 - v2
Checksum:i700B468E4D251994
GO
Isoform 2 (identifier: P15455-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-104: Missing.

Note: No experimental confirmation available.
Show »
Length:368
Mass (Da):41,032
Checksum:iA43C1373B2904EA5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti167 – 1671E → Q in AAA32777 (Ref. 1) Curated
Sequence conflicti167 – 1671E → Q in CAA32493 (Ref. 1) Curated
Sequence conflicti356 – 3561V → E in AAA32777 (Ref. 1) Curated
Sequence conflicti356 – 3561V → E in CAA32493 (Ref. 1) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 104104Missing in isoform 2. 1 PublicationVSP_026066Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M37247 Genomic DNA. Translation: AAA32777.1.
X14312 Genomic DNA. Translation: CAA32493.1.
AB005239 Genomic DNA. Translation: BAB10979.1.
CP002688 Genomic DNA. Translation: AED95062.1.
CP002688 Genomic DNA. Translation: AED95064.1.
AY070730 mRNA. Translation: AAL50071.1.
BT029491 mRNA. Translation: ABL66748.1.
AK221158 mRNA. Translation: BAD95189.1.
Z17590 mRNA. Translation: CAA79005.1.
PIRiS08509.
RefSeqiNP_199225.1. NM_123779.4. [P15455-1]
NP_851128.1. NM_180797.1. [P15455-2]
UniGeneiAt.20540.
At.74770.

Genome annotation databases

EnsemblPlantsiAT5G44120.3; AT5G44120.3; AT5G44120. [P15455-1]
GeneIDi834435.
KEGGiath:AT5G44120.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M37247 Genomic DNA. Translation: AAA32777.1.
X14312 Genomic DNA. Translation: CAA32493.1.
AB005239 Genomic DNA. Translation: BAB10979.1.
CP002688 Genomic DNA. Translation: AED95062.1.
CP002688 Genomic DNA. Translation: AED95064.1.
AY070730 mRNA. Translation: AAL50071.1.
BT029491 mRNA. Translation: ABL66748.1.
AK221158 mRNA. Translation: BAD95189.1.
Z17590 mRNA. Translation: CAA79005.1.
PIRiS08509.
RefSeqiNP_199225.1. NM_123779.4. [P15455-1]
NP_851128.1. NM_180797.1. [P15455-2]
UniGeneiAt.20540.
At.74770.

3D structure databases

ProteinModelPortaliP15455.
SMRiP15455. Positions 30-459.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19685. 3 interactions.
STRINGi3702.AT5G44120.3.

PTM databases

iPTMnetiP15455.

Proteomic databases

PaxDbiP15455.
PRIDEiP15455.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G44120.3; AT5G44120.3; AT5G44120. [P15455-1]
GeneIDi834435.
KEGGiath:AT5G44120.

Organism-specific databases

TAIRiAT5G44120.

Phylogenomic databases

eggNOGiENOG410IHSE. Eukaryota.
ENOG410YATH. LUCA.
HOGENOMiHOG000217279.
InParanoidiP15455.
OMAiHENAMIN.
OrthoDBiEOG093607KS.
PhylomeDBiP15455.

Miscellaneous databases

PROiP15455.

Gene expression databases

ExpressionAtlasiP15455. baseline and differential.
GenevisibleiP15455. AT.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
InterProiIPR022379. 11S_seedstore_CS.
IPR006044. 11S_seedstore_pln.
IPR006045. Cupin_1.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF00190. Cupin_1. 2 hits.
[Graphical view]
PRINTSiPR00439. 11SGLOBULIN.
SMARTiSM00835. Cupin_1. 2 hits.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 2 hits.
PROSITEiPS00305. 11S_SEED_STORAGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCRU1_ARATH
AccessioniPrimary (citable) accession number: P15455
Secondary accession number(s): Q3E711, Q56Z11, Q9FFH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: June 20, 2002
Last modified: September 7, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.