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Protein

Guanylate kinase

Gene

GUK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Essential for recycling GMP and indirectly, cGMP.

Catalytic activityi

ATP + GMP = ADP + GDP.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi9 – 16ATP8
Nucleotide bindingi35 – 82GMPAdd BLAST48

GO - Molecular functioni

GO - Biological processi

  • GDP biosynthetic process Source: SGD
  • purine nucleotide metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER3O-102.
YEAST:MONOMER3O-102.
BRENDAi2.7.4.8. 984.
ReactomeiR-SCE-499943. Synthesis and interconversion of nucleotide di- and triphosphates.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanylate kinase (EC:2.7.4.8)
Alternative name(s):
GMP kinase
Gene namesi
Name:GUK1
Ordered Locus Names:YDR454C
ORF Names:D9461.39
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR454C.
SGDiS000002862. GUK1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001706552 – 187Guanylate kinaseAdd BLAST186

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserine2 Publications1
Modified residuei149PhosphoserineCombined sources1
Modified residuei157PhosphotyrosineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP15454.
PRIDEiP15454.
TopDownProteomicsiP15454.

PTM databases

iPTMnetiP15454.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi32509. 29 interactors.
DIPiDIP-6721N.
MINTiMINT-522729.

Structurei

Secondary structure

1187
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 8Combined sources4
Helixi15 – 25Combined sources11
Turni27 – 29Combined sources3
Beta strandi30 – 32Combined sources3
Beta strandi35 – 37Combined sources3
Turni47 – 49Combined sources3
Beta strandi50 – 53Combined sources4
Helixi56 – 64Combined sources9
Beta strandi68 – 74Combined sources7
Beta strandi77 – 82Combined sources6
Helixi83 – 92Combined sources10
Beta strandi94 – 99Combined sources6
Helixi102 – 109Combined sources8
Helixi112 – 114Combined sources3
Beta strandi117 – 122Combined sources6
Helixi126 – 136Combined sources11
Helixi141 – 158Combined sources18
Turni159 – 161Combined sources3
Beta strandi163 – 168Combined sources6
Helixi172 – 183Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EX6X-ray2.30A/B2-187[»]
1EX7X-ray1.90A2-187[»]
1GKYX-ray2.00A2-187[»]
4F4JX-ray2.45A/B1-187[»]
ProteinModelPortaliP15454.
SMRiP15454.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15454.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 184Guanylate kinase-likeAdd BLAST183

Sequence similaritiesi

Belongs to the guanylate kinase family.Curated
Contains 1 guanylate kinase-like domain.Curated

Phylogenomic databases

GeneTreeiENSGT00840000129809.
HOGENOMiHOG000037640.
InParanoidiP15454.
KOiK00942.
OMAiMELQSIV.
OrthoDBiEOG092C4CZ3.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
HAMAPiMF_00328. Guanylate_kinase. 1 hit.
InterProiIPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like_dom.
IPR017665. Guanylate_kinase.
IPR020590. Guanylate_kinase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00625. Guanylate_kin. 1 hit.
[Graphical view]
SMARTiSM00072. GuKc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR03263. guanyl_kin. 1 hit.
PROSITEiPS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15454-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRPIVISGP SGTGKSTLLK KLFAEYPDSF GFSVSSTTRT PRAGEVNGKD
60 70 80 90 100
YNFVSVDEFK SMIKNNEFIE WAQFSGNYYG STVASVKQVS KSGKTCILDI
110 120 130 140 150
DMQGVKSVKA IPELNARFLF IAPPSVEDLK KRLEGRGTET EESINKRLSA
160 170 180
AQAELAYAET GAHDKVIVND DLDKAYKELK DFIFAEK
Length:187
Mass (Da):20,637
Last modified:January 23, 2007 - v2
Checksum:i5DC4D2A22594BFB8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L04683 Genomic DNA. Translation: AAA34657.1.
U33007 Genomic DNA. Translation: AAB64881.1.
BK006938 Genomic DNA. Translation: DAA12289.1.
PIRiA45097. KIBYGU.
RefSeqiNP_010742.1. NM_001180762.1.

Genome annotation databases

EnsemblFungiiYDR454C; YDR454C; YDR454C.
GeneIDi852065.
KEGGisce:YDR454C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L04683 Genomic DNA. Translation: AAA34657.1.
U33007 Genomic DNA. Translation: AAB64881.1.
BK006938 Genomic DNA. Translation: DAA12289.1.
PIRiA45097. KIBYGU.
RefSeqiNP_010742.1. NM_001180762.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EX6X-ray2.30A/B2-187[»]
1EX7X-ray1.90A2-187[»]
1GKYX-ray2.00A2-187[»]
4F4JX-ray2.45A/B1-187[»]
ProteinModelPortaliP15454.
SMRiP15454.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32509. 29 interactors.
DIPiDIP-6721N.
MINTiMINT-522729.

PTM databases

iPTMnetiP15454.

Proteomic databases

MaxQBiP15454.
PRIDEiP15454.
TopDownProteomicsiP15454.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR454C; YDR454C; YDR454C.
GeneIDi852065.
KEGGisce:YDR454C.

Organism-specific databases

EuPathDBiFungiDB:YDR454C.
SGDiS000002862. GUK1.

Phylogenomic databases

GeneTreeiENSGT00840000129809.
HOGENOMiHOG000037640.
InParanoidiP15454.
KOiK00942.
OMAiMELQSIV.
OrthoDBiEOG092C4CZ3.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER3O-102.
YEAST:MONOMER3O-102.
BRENDAi2.7.4.8. 984.
ReactomeiR-SCE-499943. Synthesis and interconversion of nucleotide di- and triphosphates.

Miscellaneous databases

EvolutionaryTraceiP15454.
PROiP15454.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
HAMAPiMF_00328. Guanylate_kinase. 1 hit.
InterProiIPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like_dom.
IPR017665. Guanylate_kinase.
IPR020590. Guanylate_kinase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00625. Guanylate_kin. 1 hit.
[Graphical view]
SMARTiSM00072. GuKc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR03263. guanyl_kin. 1 hit.
PROSITEiPS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKGUA_YEAST
AccessioniPrimary (citable) accession number: P15454
Secondary accession number(s): D6VT79
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 175 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 20500 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.