P15447 (GLB4_GLYDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Globin, monomeric component M-IV Alternative name(s): GMH4
Organism	Glycera dibranchiata (Bloodworm)
Taxonomic identifier	6350 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Lophotrochozoa › Annelida › Polychaeta › Palpata › Aciculata › Phyllodocida › Glyceridae › Glycera

Protein attributes

Sequence length	148 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Monomer. Ref.4
Sequence similarities	Belongs to the globin family.
Mass spectrometry	Molecular mass is 15031.6±3.8 Da from positions 2 - 148. Determined by ESI. Ref.2

Ontologies

Keywords
Biological process	Oxygen transport Transport
Ligand	Heme Iron Metal-binding
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Molecular_function	heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro oxygen binding Inferred from electronic annotation. Source: InterPro oxygen transporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.1 Ref.2

Chain

2 – 148

147

Globin, monomeric component M-IV

PRO_0000052502

Sites

Metal binding

91

1

Iron (heme axial ligand)

Secondary structure

1

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148

Helix Strand Turn

Details...

Helix

5 – 19

15

Turn

20 – 24

5

Helix

25 – 38

14

Helix

40 – 42

3

Helix

43 – 46

4

Beta strand

49 – 52

4

Helix

55 – 72

18

Turn

73 – 75

3

Helix

77 – 90

14

Helix

91 – 93

3

Beta strand

94 – 97

4

Helix

101 – 103

3

Helix

104 – 119

16

Helix

120 – 122

3

Helix

125 – 146

22

Sequences

	Sequence		Length	Mass (Da)
	P15447 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 9206F7FBE1CAB7F1	FASTA	148	15,178
	10 20 30 40 50 60 MGLSAAQRQV VASTWKDIAG SDNGAGVGKE CFTKFLSAHH DIAAVFGFSG ASDPGVADLG 70 80 90 100 110 120 AKVLAQIGVA VSHLGDEGKM VAEMKAVGVR HKGYGYKHIK AEYFEPLGAS LLSAMEHRIG 130 140 GKMTAAAKDA WAAAYADISG ALISGLQS

References

[1]	"cDNA cloning and predicted amino acid sequence of Glycera dibranchiata monomer hemoglobin IV." Simons P.C., Satterlee J.D. Biochemistry 28:8525-8530(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-25.
[2]	"Complete amino acid sequence of the Glycera dibranchiata monomer hemoglobin component IV: structural implications." Alam S.L., Satterlee J.D., Edmonds C.G. J. Protein Chem. 13:151-164(1994) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-148, MASS SPECTROMETRY, 3D-STRUCTURE MODELING. Tissue: Erythrocyte.
[3]	"Solution structure and backbone dynamics of component IV Glycera dibranchiata monomeric hemoglobin-CO." Volkman B.F., Alam S.L., Satterlee J.D., Markley J.L. Biochemistry 37:10906-10919(1998) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.
[4]	"Crystal structures of unligated and CN-ligated Glycera dibranchiata monomer ferric hemoglobin components III and IV." Park H.J., Yang C., Treff N., Satterlee J.D., Kang C. Proteins 49:49-60(2002) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.4 ANGSTROMS)IN COMPLEX WITH HEME, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J02873 mRNA. Translation: AAA29162.1.

PIR

A33420.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1JF4	X-ray	1.40	A	2-147	[»]
1JL6	X-ray	1.40	A	2-147	[»]
1VRE	NMR	-	A	2-148	[»]
1VRF	NMR	-	A	2-148	[»]

ProteinModelPortal

P15447.

SMR

P15447. Positions 2-148.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

1.10.490.10. 1 hit.

InterPro

IPR000971. Globin.
IPR009050. Globin-like.
IPR013316. Globin_annelid-type.
IPR012292. Globin_dom.
[Graphical view]

Pfam

PF00042. Globin. 1 hit.
[Graphical view]

PRINTS

PR01907. WORMGLOBIN.

SUPFAM

SSF46458. Globin_like. 1 hit.

PROSITE

PS01033. GLOBIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P15447.

Entry information

Entry name

GLB4_GLYDI

Accession

Primary (citable) accession number: P15447
Secondary accession number(s): P81779

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)