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P15446

- MTH2_HAEPA

UniProt

P15446 - MTH2_HAEPA

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Protein

Modification methylase HpaII

Gene

hpaIIM

Organism
Haemophilus parainfluenzae
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

This methylase recognizes the double-stranded sequence CCGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the HpaII endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei103 – 1031PROSITE-ProRule annotation

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3432. M.HpaII.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase HpaII (EC:2.1.1.37)
Short name:
M.HpaII
Alternative name(s):
Cytosine-specific methyltransferase HpaII
Gene namesi
Name:hpaIIM
OrganismiHaemophilus parainfluenzae
Taxonomic identifieri729 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi55 – 551C → Y in temperature-sensitive mutant.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 358358Modification methylase HpaIIPRO_0000087883Add
BLAST

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP15446.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 356325SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P15446-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKDVLDDNLL EEPAAQYSLF EPESNPNLRE KFTFIDLFAG IGGFRIAMQN
60 70 80 90 100
LGGKCIFSSE WDEQAQKTYE ANFGDLPYGD ITLEETKAFI PEKFDILCAG
110 120 130 140 150
FPCQAFSIAG KRGGFEDTRG TLFFDVAEII RRHQPKAFFL ENVKGLKNHD
160 170 180 190 200
KGRTLKTILN VLREDLGYFV PEPAIVNAKN FGVPQNRERI YIVGFHKSTG
210 220 230 240 250
VNSFSYPEPL DKIVTFADIR EEKTVPTKYY LSTQYIDTLR KHKERHESKG
260 270 280 290 300
NGFGYEIIPD DGIANAIVVG GMGRERNLVI DHRITDFTPT TNIKGEVNRE
310 320 330 340 350
GIRKMTPREW ARLQGFPDSY VIPVSDASAY KQFGNSVAVP AIQATGKKIL

EKLGNLYD
Length:358
Mass (Da):40,400
Last modified:April 1, 1990 - v1
Checksum:iACE69BFD511C37EB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X51322 Genomic DNA. Translation: CAA35705.1.
L17342 Genomic DNA. Translation: AAA20481.1.
PIRiS15908.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X51322 Genomic DNA. Translation: CAA35705.1 .
L17342 Genomic DNA. Translation: AAA20481.1 .
PIRi S15908.

3D structure databases

ProteinModelPortali P15446.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3432. M.HpaII.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF00145. DNA_methylase. 1 hit.
[Graphical view ]
PRINTSi PR00105. C5METTRFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00675. dcm. 1 hit.
PROSITEi PS00094. C5_MTASE_1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 49669.
  2. "Organization and sequence of the HpaII restriction-modification system and adjacent genes."
    Kulakauskas S., Barsomian J.M., Lubys A., Roberts R.J., Wilson G.G.
    Gene 142:9-15(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiMTH2_HAEPA
AccessioniPrimary (citable) accession number: P15446
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: October 1, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3