Reviewed, UniProtKB/Swiss-Prot P15446 (MTH2_HAEPA)

Names and origin

Protein names

Recommended name:
    Modification methylase HpaII
      Short name=M.HpaII
    EC=2.1.1.37
Alternative name(s):
    Cytosine-specific methyltransferase HpaII

Gene names

Name:

hpaIIM

Organism

Haemophilus parainfluenzae

Taxonomic identifier

729 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence CCGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the HpaII endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Subunit structure	Monomer.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 358	358	Modification methylase HpaII		PRO_0000087883
Sites
	Active site	103	1	By similarity
Experimental info
	Mutagenesis	55	1	C → Y in temperature-sensitive mutant.

Sequences

	Sequence		Length	Mass (Da)
	P15446-1 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: ACE69BFD511C37EB	FASTA	358	40,400
	10 20 30 40 50 60 MKDVLDDNLL EEPAAQYSLF EPESNPNLRE KFTFIDLFAG IGGFRIAMQN LGGKCIFSSE 70 80 90 100 110 120 WDEQAQKTYE ANFGDLPYGD ITLEETKAFI PEKFDILCAG FPCQAFSIAG KRGGFEDTRG 130 140 150 160 170 180 TLFFDVAEII RRHQPKAFFL ENVKGLKNHD KGRTLKTILN VLREDLGYFV PEPAIVNAKN 190 200 210 220 230 240 FGVPQNRERI YIVGFHKSTG VNSFSYPEPL DKIVTFADIR EEKTVPTKYY LSTQYIDTLR 250 260 270 280 290 300 KHKERHESKG NGFGYEIIPD DGIANAIVVG GMGRERNLVI DHRITDFTPT TNIKGEVNRE 310 320 330 340 350 GIRKMTPREW ARLQGFPDSY VIPVSDASAY KQFGNSVAVP AIQATGKKIL EKLGNLYD

References

[1]	"Cloning and characterization of the HpaII methylase gene." Card C.O., Wilson G.G., Weule K., Hasapes J., Kiss A., Roberts R.J. Nucleic Acids Res. 18:1377-1383(1990) [PubMed: 2183189] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 49669.
[2]	"Organization and sequence of the HpaII restriction-modification system and adjacent genes." Kulakauskas S., Barsomian J.M., Lubys A., Roberts R.J., Wilson G.G. Gene 142:9-15(1994) [PubMed: 7514149] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X51322 Genomic DNA. Translation: CAA35705.1. L17342 Genomic DNA. Translation: AAA20481.1.
PIR	S15908.
3D structure databases
HSSP	HSSP built from PDB template 6MHT based on UniProtKB P05102.
ModBase	Search...
Protein family/group databases
REBASE	3432. M.HpaII.
Enzyme and pathway databases
BRENDA	2.1.1.37. 96187.
Family and domain databases
InterPro	IPR001525. C5_DNA_meth. IPR018117. C5_DNA_meth_AS. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTH2_HAEPA

Accession

Primary (citable) accession number: P15446

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)