Reviewed,
UniProtKB/Swiss-Prot P15446 (MTH2_HAEPA)
Last modified
June 16, 2009.
Version 68.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Modification methylase HpaII Short name=M.HpaII EC=2.1.1.37 Alternative name(s): Cytosine-specific methyltransferase HpaII | ||
| Gene names |
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| Organism | Haemophilus parainfluenzae | ||
| Taxonomic identifier | 729 [NCBI] | ||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus |
Protein attributes
| Sequence length | 358 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | This methylase recognizes the double-stranded sequence CCGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the HpaII endonuclease. |
| Catalytic activity | S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine. |
| Subunit structure | Monomer. |
| Sequence similarities | Belongs to the C5-methyltransferase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Restriction system |
| Ligand | S-adenosyl-L-methionine |
| Molecular function | Methyltransferase Transferase |
| Gene Ontology (GO) | |
| Biological process | DNA methylation Inferred from electronic annotation. Source: InterPro DNA restriction-modification systemInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
Sequences
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References
| [1] | "Cloning and characterization of the HpaII methylase gene." Card C.O., Wilson G.G., Weule K., Hasapes J., Kiss A., Roberts R.J. Nucleic Acids Res. 18:1377-1383(1990) [PubMed: 2183189] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 49669. |
| [2] | "Organization and sequence of the HpaII restriction-modification system and adjacent genes." Kulakauskas S., Barsomian J.M., Lubys A., Roberts R.J., Wilson G.G. Gene 142:9-15(1994) [PubMed: 7514149] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
Cross-references
Sequence databases | |
|---|---|
| X51322 Genomic DNA. Translation: CAA35705.1. L17342 Genomic DNA. Translation: AAA20481.1. | |
| PIR | S15908. |
3D structure databases | |
| HSSP | HSSP built from PDB template 6MHT based on UniProtKB P05102. |
| ModBase | Search... |
Protein family/group databases | |
| REBASE | 3432. M.HpaII. |
Enzyme and pathway databases | |
| BRENDA | 2.1.1.37. 96187. |
Family and domain databases | |
| InterPro | IPR001525. C5_DNA_meth. IPR018117. C5_DNA_meth_AS. [Graphical view] |
| PANTHER | PTHR10629. C5_DNA_meth. 1 hit. |
| Pfam | PF00145. DNA_methylase. 1 hit. [Graphical view] |
| PRINTS | PR00105. C5METTRFRASE. |
| TIGRFAMs | TIGR00675. dcm. 1 hit. |
| PROSITE | PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. False negative. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | MTH2_HAEPA | ||||||||
| Accession | Primary (citable) accession number: P15446 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Restriction enzymes and methylases Classification of restriction enzymes and methylases and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


