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P15446

- MTH2_HAEPA

UniProt

P15446 - MTH2_HAEPA

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Protein

Modification methylase HpaII

Gene
hpaIIM
Organism
Haemophilus parainfluenzae
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

This methylase recognizes the double-stranded sequence CCGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the HpaII endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei103 – 1031 By similarity

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3432. M.HpaII.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase HpaII (EC:2.1.1.37)
Short name:
M.HpaII
Alternative name(s):
Cytosine-specific methyltransferase HpaII
Gene namesi
Name:hpaIIM
OrganismiHaemophilus parainfluenzae
Taxonomic identifieri729 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi55 – 551C → Y in temperature-sensitive mutant.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 358358Modification methylase HpaIIPRO_0000087883Add
BLAST

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP15446.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 356325SAM-dependent MTase C5-typeAdd
BLAST

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P15446-1 [UniParc]FASTAAdd to Basket

« Hide

MKDVLDDNLL EEPAAQYSLF EPESNPNLRE KFTFIDLFAG IGGFRIAMQN    50
LGGKCIFSSE WDEQAQKTYE ANFGDLPYGD ITLEETKAFI PEKFDILCAG 100
FPCQAFSIAG KRGGFEDTRG TLFFDVAEII RRHQPKAFFL ENVKGLKNHD 150
KGRTLKTILN VLREDLGYFV PEPAIVNAKN FGVPQNRERI YIVGFHKSTG 200
VNSFSYPEPL DKIVTFADIR EEKTVPTKYY LSTQYIDTLR KHKERHESKG 250
NGFGYEIIPD DGIANAIVVG GMGRERNLVI DHRITDFTPT TNIKGEVNRE 300
GIRKMTPREW ARLQGFPDSY VIPVSDASAY KQFGNSVAVP AIQATGKKIL 350
EKLGNLYD 358
Length:358
Mass (Da):40,400
Last modified:April 1, 1990 - v1
Checksum:iACE69BFD511C37EB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X51322 Genomic DNA. Translation: CAA35705.1.
L17342 Genomic DNA. Translation: AAA20481.1.
PIRiS15908.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X51322 Genomic DNA. Translation: CAA35705.1 .
L17342 Genomic DNA. Translation: AAA20481.1 .
PIRi S15908.

3D structure databases

ProteinModelPortali P15446.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3432. M.HpaII.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF00145. DNA_methylase. 1 hit.
[Graphical view ]
PRINTSi PR00105. C5METTRFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00675. dcm. 1 hit.
PROSITEi PS00094. C5_MTASE_1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 49669.
  2. "Organization and sequence of the HpaII restriction-modification system and adjacent genes."
    Kulakauskas S., Barsomian J.M., Lubys A., Roberts R.J., Wilson G.G.
    Gene 142:9-15(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiMTH2_HAEPA
AccessioniPrimary (citable) accession number: P15446
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: June 11, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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