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Protein

Modification methylase HpaII

Gene

hpaIIM

Organism
Haemophilus parainfluenzae
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This methylase recognizes the double-stranded sequence CCGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the HpaII endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei103PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processRestriction system
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase HpaII (EC:2.1.1.37)
Short name:
M.HpaII
Alternative name(s):
Cytosine-specific methyltransferase HpaII
Gene namesi
Name:hpaIIM
OrganismiHaemophilus parainfluenzae
Taxonomic identifieri729 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi55C → Y in temperature-sensitive mutant. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000878831 – 358Modification methylase HpaIIAdd BLAST358

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP15446
SMRiP15446
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 356SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST325

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Family and domain databases

InterProiView protein in InterPro
IPR018117 C5_DNA_meth_AS
IPR001525 C5_MeTfrase
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF00145 DNA_methylase, 1 hit
PRINTSiPR00105 C5METTRFRASE
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00675 dcm, 1 hit
PROSITEiView protein in PROSITE
PS00094 C5_MTASE_1, 1 hit
PS51679 SAM_MT_C5, 1 hit

Sequencei

Sequence statusi: Complete.

P15446-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDVLDDNLL EEPAAQYSLF EPESNPNLRE KFTFIDLFAG IGGFRIAMQN
60 70 80 90 100
LGGKCIFSSE WDEQAQKTYE ANFGDLPYGD ITLEETKAFI PEKFDILCAG
110 120 130 140 150
FPCQAFSIAG KRGGFEDTRG TLFFDVAEII RRHQPKAFFL ENVKGLKNHD
160 170 180 190 200
KGRTLKTILN VLREDLGYFV PEPAIVNAKN FGVPQNRERI YIVGFHKSTG
210 220 230 240 250
VNSFSYPEPL DKIVTFADIR EEKTVPTKYY LSTQYIDTLR KHKERHESKG
260 270 280 290 300
NGFGYEIIPD DGIANAIVVG GMGRERNLVI DHRITDFTPT TNIKGEVNRE
310 320 330 340 350
GIRKMTPREW ARLQGFPDSY VIPVSDASAY KQFGNSVAVP AIQATGKKIL

EKLGNLYD
Length:358
Mass (Da):40,400
Last modified:April 1, 1990 - v1
Checksum:iACE69BFD511C37EB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51322 Genomic DNA Translation: CAA35705.1
L17342 Genomic DNA Translation: AAA20481.1
PIRiS15908

Similar proteinsi

Entry informationi

Entry nameiMTH2_HAEPA
AccessioniPrimary (citable) accession number: P15446
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: March 28, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health