P15445 (PA2_NAJNA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Phospholipase A2, acidic EC=3.1.1.4 Alternative name(s): Phosphatidylcholine 2-acylhydrolase Phospholipase A2 2 Short name=PLA22
Organism	Naja naja (Indian cobra)
Taxonomic identifier	35670 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Scleroglossa › Serpentes › Colubroidea › Elapidae › Elapinae › Naja

Protein attributes

Sequence length	119 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Like most other acidic phospholipases, this protein is not neurotoxic.
Catalytic activity	Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
Cofactor	Binds 1 calcium ion per subunit.
Subunit structure	Homotrimer.
Subcellular location	Secreted Ref.3.
Tissue specificity	Expressed by the venom gland. Ref.3
Sequence similarities	Belongs to the phospholipase A2 family. Group I subfamily.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Secreted
Ligand	Calcium Metal-binding
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW phospholipid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro phospholipase A2 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 119

119

Phospholipase A2, acidic

PRO_0000161669

Sites

Active site

47

1

Active site

93

1

Metal binding

27

1

Calcium; via carbonyl oxygen

Metal binding

29

1

Calcium; via carbonyl oxygen

Metal binding

31

1

Calcium; via carbonyl oxygen

Metal binding

48

1

Calcium Ref.4

Amino acid modifications

Disulfide bond

11 ↔ 71

Ref.4

Disulfide bond

26 ↔ 118

Ref.4

Disulfide bond

28 ↔ 44

Ref.4

Disulfide bond

43 ↔ 99

Ref.4

Disulfide bond

50 ↔ 92

Ref.4

Disulfide bond

60 ↔ 85

Ref.4

Disulfide bond

78 ↔ 90

Ref.4

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

119

Helix Strand Turn

Details...

Helix

2 – 12

11

Helix

18 – 21

4

Beta strand

22 – 24

3

Turn

25 – 27

3

Beta strand

28 – 30

3

Helix

39 – 54

16

Turn

62 – 64

3

Beta strand

69 – 72

4

Beta strand

75 – 78

4

Helix

84 – 102

19

Helix

107 – 109

3

Helix

114 – 117

4

Sequences

	Sequence		Length	Mass (Da)
	P15445 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: B725291AAB522966	FASTA	119	13,346
	10 20 30 40 50 60 NLYQFKNMIK CTVPSRSWWD FADYGCYCGR GGSGTPVDDL DRCCQVHDNC YNEAEKISGC 70 80 90 100 110 WPYFKTYSYE CSQGTLTCKG DNNACAASVC DCDRLAAICF AGAPYNDNNY NIDLKARCQ

References

[1]	"Amino acid sequence and circular dichroism of Indian cobra (Naja naja naja) venom acidic phospholipase A2." Davidson F.F., Dennis E.A. Biochim. Biophys. Acta 1037:7-15(1990) [PubMed: 2294972] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Venom.
[2]	"Phospholipase A2 from cobra (Naja naja naja) venom. Primary structure and subspecies variation." Shafqat J., Beg O.U., Joernvall H., Zaidi Z.H. Protein Seq. Data Anal. 2:451-452(1989) [PubMed: 2626425] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Venom.
[3]	"Molecular diversity in venom proteins of the Russell's viper (Daboia russellii russellii) and the Indian cobra (Naja naja) in Sri Lanka." Suzuki M., Itoh T., Bandaranayake B.M.A.I.K., Ranasinghe J.G., Athauda S.B., Moriyama A. Biomed. Res. 31:71-81(2010) [PubMed: 20203422] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-20, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Venom.
[4]	"Crystal structure of phospholipase A2 from Indian cobra reveals a trimeric association." Fremont D.H., Anderson D.H., Wilson I.A., Dennis E.A., Xuong N.-H. Proc. Natl. Acad. Sci. U.S.A. 90:342-346(1993) [PubMed: 8419939] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

PIR

S07528.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1A3D	X-ray	1.80	A	1-119	[»]
1A3F	X-ray	2.65	A/B/C	1-119	[»]
1OWS	X-ray	2.30	A	3-118	[»]
			B	4-119	[»]
1PSH	X-ray	2.30	A/B/C	1-119	[»]
2WQ5	X-ray	1.65	A	1-119	[»]

ProteinModelPortal

P15445.

SMR

P15445. Positions 1-119.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Phylogenomic databases

HOVERGEN

HBG008137.

Family and domain databases

InterPro

IPR016090. PLipase_A2.
IPR013090. PLipase_A2_AS.
IPR001211. PLipase_A2_euk.
[Graphical view]

Gene3D

G3DSA:1.20.90.10. Phospholipase_A2. 1 hit.

PANTHER

PTHR11716. Phospholipase_A2. 1 hit.

Pfam

PF00068. Phospholip_A2_1. 1 hit.
[Graphical view]

PRINTS

PR00389. PHPHLIPASEA2.

SMART

SM00085. PA2c. 1 hit.
[Graphical view]

SUPFAM

SSF48619. PhospholipaseA2. 1 hit.

PROSITE

PS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

PA2_NAJNA

Accession

Primary (citable) accession number: P15445

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	October 19, 2011
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program