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P15431

- GBRB1_RAT

UniProt

P15431 - GBRB1_RAT

Protein

Gamma-aminobutyric acid receptor subunit beta-1

Gene

Gabrb1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 1 (01 Apr 1990)
      Previous versions | rss
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    Functioni

    Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei225 – 2251AgonistBy similarity

    GO - Molecular functioni

    1. anion channel activity Source: RGD
    2. chloride channel activity Source: UniProtKB-KW
    3. extracellular ligand-gated ion channel activity Source: InterPro
    4. GABA-A receptor activity Source: UniProtKB
    5. GABA receptor binding Source: RGD
    6. ligand-gated ion channel activity Source: UniProtKB

    GO - Biological processi

    1. cellular response to histamine Source: UniProtKB
    2. central nervous system neuron development Source: RGD
    3. chloride transport Source: RGD
    4. gamma-aminobutyric acid signaling pathway Source: RGD
    5. ion transmembrane transport Source: GOC
    6. ion transport Source: UniProtKB
    7. ovulation cycle Source: RGD
    8. response to progesterone Source: RGD
    9. response to toxic substance Source: RGD

    Keywords - Molecular functioni

    Chloride channel, Ion channel, Ligand-gated ion channel, Receptor

    Keywords - Biological processi

    Ion transport, Transport

    Keywords - Ligandi

    Chloride

    Enzyme and pathway databases

    ReactomeiREACT_199021. GABA A receptor activation.
    REACT_199029. Ligand-gated ion channel transport.

    Protein family/group databases

    TCDBi1.A.9.5.1. the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Gamma-aminobutyric acid receptor subunit beta-1
    Alternative name(s):
    GABA(A) receptor subunit beta-1
    Gene namesi
    Name:Gabrb1
    Synonyms:Gabrb-1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi2649. Gabrb1.

    Subcellular locationi

    Cell junctionsynapsepostsynaptic cell membrane 1 Publication; Multi-pass membrane protein 1 Publication. Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. chloride channel complex Source: UniProtKB-KW
    3. cytoplasm Source: RGD
    4. dendrite Source: RGD
    5. GABA-A receptor complex Source: UniProtKB
    6. integral component of plasma membrane Source: UniProtKB
    7. membrane Source: RGD
    8. nuclear envelope Source: RGD
    9. nucleus Source: RGD
    10. postsynaptic membrane Source: UniProtKB-SubCell
    11. receptor complex Source: RGD

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525Sequence AnalysisAdd
    BLAST
    Chaini26 – 474449Gamma-aminobutyric acid receptor subunit beta-1PRO_0000000458Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi33 – 331N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi105 – 1051N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi161 ↔ 175By similarity
    Glycosylationi174 – 1741N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiP15431.
    PRIDEiP15431.

    PTM databases

    PhosphoSiteiP15431.

    Expressioni

    Gene expression databases

    GenevestigatoriP15431.

    Interactioni

    Subunit structurei

    Binds UBQLN1. Interacts with KCTD8, KCTD12 and KCTD16; this interaction determines the pharmacology and kinetics of the receptor response, the KCTD proteins markedly accelerating the GABA-B response, although to different extents By similarity. Heteropentamer, formed by a combination of alpha, beta, gamma, delta and rho chains.By similarity1 Publication

    Protein-protein interaction databases

    IntActiP15431. 2 interactions.
    MINTiMINT-219840.
    STRINGi10116.ENSRNOP00000003170.

    Structurei

    3D structure databases

    ProteinModelPortaliP15431.
    SMRiP15431. Positions 274-330.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini26 – 245220ExtracellularCuratedAdd
    BLAST
    Topological domaini328 – 451124CytoplasmicCuratedAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei246 – 26722HelicalCuratedAdd
    BLAST
    Transmembranei271 – 29323HelicalCuratedAdd
    BLAST
    Transmembranei305 – 32723HelicalCuratedAdd
    BLAST
    Transmembranei452 – 47322HelicalCuratedAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni120 – 1223Agonist bindingBy similarity
    Regioni180 – 1823Agonist bindingBy similarity
    Regioni290 – 31122Allosteric effector bindingBy similarityAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG77438.
    HOGENOMiHOG000231335.
    HOVERGENiHBG051707.
    InParanoidiP15431.
    KOiK05181.
    OrthoDBiEOG712TVZ.
    PhylomeDBiP15431.
    TreeFamiTF315453.

    Family and domain databases

    Gene3Di2.70.170.10. 1 hit.
    InterProiIPR006028. GABAA/Glycine_rcpt.
    IPR002289. GABAAb_rcpt.
    IPR006202. Neur_chan_lig-bd.
    IPR006201. Neur_channel.
    IPR006029. Neurotrans-gated_channel_TM.
    IPR018000. Neurotransmitter_ion_chnl_CS.
    [Graphical view]
    PANTHERiPTHR18945. PTHR18945. 1 hit.
    PfamiPF02931. Neur_chan_LBD. 1 hit.
    PF02932. Neur_chan_memb. 1 hit.
    [Graphical view]
    PRINTSiPR01160. GABAARBETA.
    PR00253. GABAARECEPTR.
    PR00252. NRIONCHANNEL.
    SUPFAMiSSF63712. SSF63712. 1 hit.
    SSF90112. SSF90112. 1 hit.
    TIGRFAMsiTIGR00860. LIC. 1 hit.
    PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P15431-1 [UniParc]FASTAAdd to Basket

    « Hide

    MWTVQNRESL GLLSFPVMVA MVCCAHSSNE PSNMSYVKET VDRLLKGYDI    50
    RLRPDFGGPP VDVGMRIDVA SIDMVSEVNM DYTLTMYFQQ SWKDKRLSYS 100
    GIPLNLTLDN RVADQLWVPD TYFLNDKKSF VHGVTVKNRM IRLHPDGTVL 150
    YGLRITTTAA CMMDLRRYPL DEQNCTLEIE SYGYTTDDIE FYWNGGEGAV 200
    TGVNKIELPQ FSIVDYKMVS KKVEFTTGAY PRLSLSFRLK RNIGYFILQT 250
    YMPSTLITIL SWVSFWINYD ASAARVALGI TTVLTMTTIS THLRETLPKI 300
    PYVKAIDIYL MGCFVFVFLA LLEYAFVNYI FFGKGPQKKG ASKQDQSANE 350
    KNKLEMNKVQ VDAHGNILLS TLEIRNETSG SEVLTGVSDP KATMYSYDSA 400
    SIQYRKPLSS REGFGRGLDR HGVPGKGRIR RRASQLKVKI PDLTDVNSID 450
    KWSRMFFPIT FSLFNVVYWL YYVH 474
    Length:474
    Mass (Da):54,072
    Last modified:April 1, 1990 - v1
    Checksum:i986277FDBD66377A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti420 – 4201R → Q no nucleotide entry (PubMed:1977069)Curated
    Sequence conflicti428 – 4281R → P no nucleotide entry (PubMed:1977069)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15466 mRNA. Translation: CAA33493.1.
    PIRiS04464. B60039.
    RefSeqiNP_037088.1. NM_012956.1.
    UniGeneiRn.207157.

    Genome annotation databases

    GeneIDi25450.
    KEGGirno:25450.
    UCSCiRGD:2649. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15466 mRNA. Translation: CAA33493.1 .
    PIRi S04464. B60039.
    RefSeqi NP_037088.1. NM_012956.1.
    UniGenei Rn.207157.

    3D structure databases

    ProteinModelPortali P15431.
    SMRi P15431. Positions 274-330.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P15431. 2 interactions.
    MINTi MINT-219840.
    STRINGi 10116.ENSRNOP00000003170.

    Chemistry

    BindingDBi P15431.
    ChEMBLi CHEMBL2096683.
    GuidetoPHARMACOLOGYi 410.

    Protein family/group databases

    TCDBi 1.A.9.5.1. the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family.

    PTM databases

    PhosphoSitei P15431.

    Proteomic databases

    PaxDbi P15431.
    PRIDEi P15431.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 25450.
    KEGGi rno:25450.
    UCSCi RGD:2649. rat.

    Organism-specific databases

    CTDi 2560.
    RGDi 2649. Gabrb1.

    Phylogenomic databases

    eggNOGi NOG77438.
    HOGENOMi HOG000231335.
    HOVERGENi HBG051707.
    InParanoidi P15431.
    KOi K05181.
    OrthoDBi EOG712TVZ.
    PhylomeDBi P15431.
    TreeFami TF315453.

    Enzyme and pathway databases

    Reactomei REACT_199021. GABA A receptor activation.
    REACT_199029. Ligand-gated ion channel transport.

    Miscellaneous databases

    NextBioi 606697.
    PROi P15431.

    Gene expression databases

    Genevestigatori P15431.

    Family and domain databases

    Gene3Di 2.70.170.10. 1 hit.
    InterProi IPR006028. GABAA/Glycine_rcpt.
    IPR002289. GABAAb_rcpt.
    IPR006202. Neur_chan_lig-bd.
    IPR006201. Neur_channel.
    IPR006029. Neurotrans-gated_channel_TM.
    IPR018000. Neurotransmitter_ion_chnl_CS.
    [Graphical view ]
    PANTHERi PTHR18945. PTHR18945. 1 hit.
    Pfami PF02931. Neur_chan_LBD. 1 hit.
    PF02932. Neur_chan_memb. 1 hit.
    [Graphical view ]
    PRINTSi PR01160. GABAARBETA.
    PR00253. GABAARECEPTR.
    PR00252. NRIONCHANNEL.
    SUPFAMi SSF63712. SSF63712. 1 hit.
    SSF90112. SSF90112. 1 hit.
    TIGRFAMsi TIGR00860. LIC. 1 hit.
    PROSITEi PS00236. NEUROTR_ION_CHANNEL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "GABAA receptor beta subunit heterogeneity: functional expression of cloned cDNAs."
      Ymer S., Schofield P.R., Draguhn A., Werner P., Koehler M., Seeburg P.H.
      EMBO J. 8:1665-1670(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "GABAA-receptor expressed from rat brain alpha- and beta-subunit cDNAs displays potentiation by benzodiazepine receptor ligands."
      Malherbe P., Draguhn A., Multhaup G., Beyreuther K., Mohler H.
      Brain Res. Mol. Brain Res. 8:199-208(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    3. "Histamine action on vertebrate GABAA receptors: direct channel gating and potentiation of GABA responses."
      Saras A., Gisselmann G., Vogt-Eisele A.K., Erlkamp K.S., Kletke O., Pusch H., Hatt H.
      J. Biol. Chem. 283:10470-10475(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, SUBUNIT.

    Entry informationi

    Entry nameiGBRB1_RAT
    AccessioniPrimary (citable) accession number: P15431
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: April 1, 1990
    Last modified: October 1, 2014
    This is version 130 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3