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Protein

Gamma-aminobutyric acid receptor subunit beta-1

Gene

Gabrb1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei225AgonistBy similarity1

GO - Molecular functioni

  • anion channel activity Source: RGD
  • GABA-A receptor activity Source: RGD
  • GABA-gated chloride ion channel activity Source: UniProtKB
  • GABA receptor binding Source: RGD
  • ligand-gated ion channel activity Source: UniProtKB

GO - Biological processi

  • cellular response to histamine Source: UniProtKB
  • central nervous system neuron development Source: RGD
  • chloride transport Source: RGD
  • gamma-aminobutyric acid signaling pathway Source: UniProtKB
  • ion transport Source: UniProtKB
  • ovulation cycle Source: RGD
  • response to progesterone Source: RGD
  • response to toxic substance Source: RGD

Keywordsi

Molecular functionChloride channel, Ion channel, Ligand-gated ion channel, Receptor
Biological processIon transport, Transport
LigandChloride

Enzyme and pathway databases

ReactomeiR-RNO-975298. Ligand-gated ion channel transport.
R-RNO-977441. GABA A receptor activation.

Protein family/group databases

TCDBi1.A.9.5.1. the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-aminobutyric acid receptor subunit beta-1
Alternative name(s):
GABA(A) receptor subunit beta-1
Gene namesi
Name:Gabrb1
Synonyms:Gabrb-1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi2649. Gabrb1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini26 – 245ExtracellularCuratedAdd BLAST220
Transmembranei246 – 267HelicalCuratedAdd BLAST22
Transmembranei271 – 293HelicalCuratedAdd BLAST23
Transmembranei305 – 327HelicalCuratedAdd BLAST23
Topological domaini328 – 451CytoplasmicCuratedAdd BLAST124
Transmembranei452 – 473HelicalCuratedAdd BLAST22

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1907607.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000000045826 – 474Gamma-aminobutyric acid receptor subunit beta-1Add BLAST449

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi33N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi105N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi161 ↔ 175By similarity
Glycosylationi174N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP15431.

PTM databases

iPTMnetiP15431.
PhosphoSitePlusiP15431.

Expressioni

Gene expression databases

BgeeiENSRNOG00000002327.
GenevisibleiP15431. RN.

Interactioni

Subunit structurei

Binds UBQLN1. Interacts with KCTD8, KCTD12 and KCTD16; this interaction determines the pharmacology and kinetics of the receptor response, the KCTD proteins markedly accelerating the GABA-B response, although to different extents (By similarity). Heteropentamer, formed by a combination of alpha, beta, gamma, delta and rho chains.By similarity1 Publication

GO - Molecular functioni

  • GABA receptor binding Source: RGD

Protein-protein interaction databases

IntActiP15431. 2 interactors.
MINTiMINT-219840.
STRINGi10116.ENSRNOP00000003170.

Structurei

3D structure databases

ProteinModelPortaliP15431.
SMRiP15431.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni120 – 122Agonist bindingBy similarity3
Regioni180 – 182Agonist bindingBy similarity3
Regioni290 – 311Allosteric effector bindingBy similarityAdd BLAST22

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00760000118821.
HOGENOMiHOG000231335.
HOVERGENiHBG051707.
InParanoidiP15431.
KOiK05181.
OMAiNAGYWIF.
OrthoDBiEOG091G0805.
PhylomeDBiP15431.
TreeFamiTF315453.

Family and domain databases

Gene3Di1.20.58.390. 1 hit.
2.70.170.10. 1 hit.
InterProiView protein in InterPro
IPR006028. GABAA/Glycine_rcpt.
IPR002289. GABAAb_rcpt.
IPR006202. Neur_chan_lig-bd.
IPR036734. Neur_chan_lig-bd_sf.
IPR006201. Neur_channel.
IPR036719. Neuro-gated_channel_TM_sf.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiView protein in Pfam
PF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
PRINTSiPR01160. GABAARBETA.
PR00253. GABAARECEPTR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiView protein in PROSITE
PS00236. NEUROTR_ION_CHANNEL. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15431-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWTVQNRESL GLLSFPVMVA MVCCAHSSNE PSNMSYVKET VDRLLKGYDI
60 70 80 90 100
RLRPDFGGPP VDVGMRIDVA SIDMVSEVNM DYTLTMYFQQ SWKDKRLSYS
110 120 130 140 150
GIPLNLTLDN RVADQLWVPD TYFLNDKKSF VHGVTVKNRM IRLHPDGTVL
160 170 180 190 200
YGLRITTTAA CMMDLRRYPL DEQNCTLEIE SYGYTTDDIE FYWNGGEGAV
210 220 230 240 250
TGVNKIELPQ FSIVDYKMVS KKVEFTTGAY PRLSLSFRLK RNIGYFILQT
260 270 280 290 300
YMPSTLITIL SWVSFWINYD ASAARVALGI TTVLTMTTIS THLRETLPKI
310 320 330 340 350
PYVKAIDIYL MGCFVFVFLA LLEYAFVNYI FFGKGPQKKG ASKQDQSANE
360 370 380 390 400
KNKLEMNKVQ VDAHGNILLS TLEIRNETSG SEVLTGVSDP KATMYSYDSA
410 420 430 440 450
SIQYRKPLSS REGFGRGLDR HGVPGKGRIR RRASQLKVKI PDLTDVNSID
460 470
KWSRMFFPIT FSLFNVVYWL YYVH
Length:474
Mass (Da):54,072
Last modified:April 1, 1990 - v1
Checksum:i986277FDBD66377A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti420R → Q no nucleotide entry (PubMed:1977069).Curated1
Sequence conflicti428R → P no nucleotide entry (PubMed:1977069).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15466 mRNA. Translation: CAA33493.1.
PIRiS04464. B60039.
RefSeqiNP_037088.1. NM_012956.1.
UniGeneiRn.207157.

Genome annotation databases

EnsembliENSRNOT00000003170; ENSRNOP00000003170; ENSRNOG00000002327.
GeneIDi25450.
KEGGirno:25450.
UCSCiRGD:2649. rat.

Similar proteinsi

Entry informationi

Entry nameiGBRB1_RAT
AccessioniPrimary (citable) accession number: P15431
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: October 25, 2017
This is version 157 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families