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Protein

Gamma-aminobutyric acid receptor subunit beta-1

Gene

Gabrb1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei225 – 2251AgonistBy similarity

GO - Molecular functioni

  1. anion channel activity Source: RGD
  2. chloride channel activity Source: UniProtKB-KW
  3. extracellular ligand-gated ion channel activity Source: InterPro
  4. GABA-A receptor activity Source: UniProtKB
  5. GABA receptor binding Source: RGD
  6. ligand-gated ion channel activity Source: UniProtKB

GO - Biological processi

  1. anion transmembrane transport Source: GOC
  2. cellular response to histamine Source: UniProtKB
  3. central nervous system neuron development Source: RGD
  4. chloride transport Source: RGD
  5. gamma-aminobutyric acid signaling pathway Source: RGD
  6. ion transmembrane transport Source: GOC
  7. ion transport Source: UniProtKB
  8. ovulation cycle Source: RGD
  9. response to progesterone Source: RGD
  10. response to toxic substance Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Protein family/group databases

TCDBi1.A.9.5.1. the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-aminobutyric acid receptor subunit beta-1
Alternative name(s):
GABA(A) receptor subunit beta-1
Gene namesi
Name:Gabrb1
Synonyms:Gabrb-1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2649. Gabrb1.

Subcellular locationi

Cell junctionsynapsepostsynaptic cell membrane 1 Publication; Multi-pass membrane protein 1 Publication. Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini26 – 245220ExtracellularCuratedAdd
BLAST
Transmembranei246 – 26722HelicalCuratedAdd
BLAST
Transmembranei271 – 29323HelicalCuratedAdd
BLAST
Transmembranei305 – 32723HelicalCuratedAdd
BLAST
Topological domaini328 – 451124CytoplasmicCuratedAdd
BLAST
Transmembranei452 – 47322HelicalCuratedAdd
BLAST

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. chloride channel complex Source: UniProtKB-KW
  3. cytoplasm Source: RGD
  4. dendrite Source: RGD
  5. GABA-A receptor complex Source: UniProtKB
  6. integral component of plasma membrane Source: UniProtKB
  7. membrane Source: RGD
  8. nuclear envelope Source: RGD
  9. nucleus Source: RGD
  10. postsynaptic membrane Source: UniProtKB-SubCell
  11. receptor complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Chaini26 – 474449Gamma-aminobutyric acid receptor subunit beta-1PRO_0000000458Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi33 – 331N-linked (GlcNAc...)Sequence Analysis
Glycosylationi105 – 1051N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi161 ↔ 175By similarity
Glycosylationi174 – 1741N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP15431.
PRIDEiP15431.

PTM databases

PhosphoSiteiP15431.

Expressioni

Gene expression databases

GenevestigatoriP15431.

Interactioni

Subunit structurei

Binds UBQLN1. Interacts with KCTD8, KCTD12 and KCTD16; this interaction determines the pharmacology and kinetics of the receptor response, the KCTD proteins markedly accelerating the GABA-B response, although to different extents (By similarity). Heteropentamer, formed by a combination of alpha, beta, gamma, delta and rho chains.By similarity1 Publication

Protein-protein interaction databases

IntActiP15431. 2 interactions.
MINTiMINT-219840.
STRINGi10116.ENSRNOP00000003170.

Structurei

3D structure databases

SMRiP15431. Positions 274-330.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni120 – 1223Agonist bindingBy similarity
Regioni180 – 1823Agonist bindingBy similarity
Regioni290 – 31122Allosteric effector bindingBy similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG77438.
HOGENOMiHOG000231335.
HOVERGENiHBG051707.
InParanoidiP15431.
KOiK05181.
OrthoDBiEOG712TVZ.
PhylomeDBiP15431.
TreeFamiTF315453.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR002289. GABAAb_rcpt.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR01160. GABAARBETA.
PR00253. GABAARECEPTR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15431-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWTVQNRESL GLLSFPVMVA MVCCAHSSNE PSNMSYVKET VDRLLKGYDI
60 70 80 90 100
RLRPDFGGPP VDVGMRIDVA SIDMVSEVNM DYTLTMYFQQ SWKDKRLSYS
110 120 130 140 150
GIPLNLTLDN RVADQLWVPD TYFLNDKKSF VHGVTVKNRM IRLHPDGTVL
160 170 180 190 200
YGLRITTTAA CMMDLRRYPL DEQNCTLEIE SYGYTTDDIE FYWNGGEGAV
210 220 230 240 250
TGVNKIELPQ FSIVDYKMVS KKVEFTTGAY PRLSLSFRLK RNIGYFILQT
260 270 280 290 300
YMPSTLITIL SWVSFWINYD ASAARVALGI TTVLTMTTIS THLRETLPKI
310 320 330 340 350
PYVKAIDIYL MGCFVFVFLA LLEYAFVNYI FFGKGPQKKG ASKQDQSANE
360 370 380 390 400
KNKLEMNKVQ VDAHGNILLS TLEIRNETSG SEVLTGVSDP KATMYSYDSA
410 420 430 440 450
SIQYRKPLSS REGFGRGLDR HGVPGKGRIR RRASQLKVKI PDLTDVNSID
460 470
KWSRMFFPIT FSLFNVVYWL YYVH
Length:474
Mass (Da):54,072
Last modified:March 31, 1990 - v1
Checksum:i986277FDBD66377A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti420 – 4201R → Q no nucleotide entry (PubMed:1977069).Curated
Sequence conflicti428 – 4281R → P no nucleotide entry (PubMed:1977069).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15466 mRNA. Translation: CAA33493.1.
PIRiS04464. B60039.
RefSeqiNP_037088.1. NM_012956.1.
UniGeneiRn.207157.

Genome annotation databases

GeneIDi25450.
KEGGirno:25450.
UCSCiRGD:2649. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15466 mRNA. Translation: CAA33493.1.
PIRiS04464. B60039.
RefSeqiNP_037088.1. NM_012956.1.
UniGeneiRn.207157.

3D structure databases

SMRiP15431. Positions 274-330.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP15431. 2 interactions.
MINTiMINT-219840.
STRINGi10116.ENSRNOP00000003170.

Chemistry

ChEMBLiCHEMBL2096683.
GuidetoPHARMACOLOGYi410.

Protein family/group databases

TCDBi1.A.9.5.1. the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family.

PTM databases

PhosphoSiteiP15431.

Proteomic databases

PaxDbiP15431.
PRIDEiP15431.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25450.
KEGGirno:25450.
UCSCiRGD:2649. rat.

Organism-specific databases

CTDi2560.
RGDi2649. Gabrb1.

Phylogenomic databases

eggNOGiNOG77438.
HOGENOMiHOG000231335.
HOVERGENiHBG051707.
InParanoidiP15431.
KOiK05181.
OrthoDBiEOG712TVZ.
PhylomeDBiP15431.
TreeFamiTF315453.

Miscellaneous databases

NextBioi606697.
PROiP15431.

Gene expression databases

GenevestigatoriP15431.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR002289. GABAAb_rcpt.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR01160. GABAARBETA.
PR00253. GABAARECEPTR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "GABAA receptor beta subunit heterogeneity: functional expression of cloned cDNAs."
    Ymer S., Schofield P.R., Draguhn A., Werner P., Koehler M., Seeburg P.H.
    EMBO J. 8:1665-1670(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "GABAA-receptor expressed from rat brain alpha- and beta-subunit cDNAs displays potentiation by benzodiazepine receptor ligands."
    Malherbe P., Draguhn A., Multhaup G., Beyreuther K., Mohler H.
    Brain Res. Mol. Brain Res. 8:199-208(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
  3. "Histamine action on vertebrate GABAA receptors: direct channel gating and potentiation of GABA responses."
    Saras A., Gisselmann G., Vogt-Eisele A.K., Erlkamp K.S., Kletke O., Pusch H., Hatt H.
    J. Biol. Chem. 283:10470-10475(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, SUBUNIT.

Entry informationi

Entry nameiGBRB1_RAT
AccessioniPrimary (citable) accession number: P15431
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 31, 1990
Last sequence update: March 31, 1990
Last modified: March 31, 2015
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.