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P15431 (GBRB1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Gamma-aminobutyric acid receptor subunit beta-1
Alternative name(s):
GABA(A) receptor subunit beta-1
Gene names
Name:Gabrb1
Synonyms:Gabrb-1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length474 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. Ref.2

Subunit structure

Binds UBQLN1 By similarity. Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho. Interacts with KCTD8, KCTD12 and KCTD16; this interaction determines the pharmacology and kinetics of the receptor response, the KCTD proteins markedly accelerating the GABA-B response, although to different extents By similarity.

Subcellular location

Cell junctionsynapsepostsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRB1 sub-subfamily. [View classification]

Ontologies

Keywords
   Biological processIon transport
Transport
   Cellular componentCell junction
Cell membrane
Membrane
Postsynaptic cell membrane
Synapse
   DomainSignal
Transmembrane
Transmembrane helix
   LigandChloride
   Molecular functionChloride channel
Ion channel
Ligand-gated ion channel
Receptor
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcentral nervous system neuron development

Inferred from expression pattern PubMed 15158077. Source: RGD

chloride transport

Traceable author statement PubMed 12239220. Source: RGD

gamma-aminobutyric acid signaling pathway

Traceable author statement PubMed 11986365. Source: RGD

ion transmembrane transport

Traceable author statement PubMed 12239220. Source: GOC

ovulation cycle

Inferred from expression pattern PubMed 16226387. Source: RGD

response to progesterone

Inferred from expression pattern PubMed 15834956. Source: RGD

response to toxic substance

Inferred from direct assay PubMed 2479580. Source: RGD

   Cellular_componentcell junction

Inferred from electronic annotation. Source: UniProtKB-KW

chloride channel complex

Inferred from electronic annotation. Source: UniProtKB-KW

cytoplasm

Inferred from direct assay PubMed 16226387. Source: RGD

dendrite

Inferred from direct assay PubMed 16226387. Source: RGD

membrane

Inferred from direct assay PubMed 17959749. Source: RGD

nuclear envelope

Inferred from direct assay PubMed 20511229. Source: RGD

nucleus

Inferred from direct assay PubMed 20511229. Source: RGD

postsynaptic membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

receptor complex

Inferred from direct assay PubMed 17959749. Source: RGD

   Molecular_functionGABA receptor binding

Inferred from physical interaction PubMed 17959749. Source: RGD

GABA-A receptor activity

Inferred from direct assay Ref.1. Source: RGD

anion channel activity

Inferred from direct assay PubMed 2479580. Source: RGD

chloride channel activity

Inferred from electronic annotation. Source: UniProtKB-KW

extracellular ligand-gated ion channel activity

Inferred from electronic annotation. Source: InterPro

ligand-gated ion channel activity

Traceable author statement PubMed 12239220. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 474449Gamma-aminobutyric acid receptor subunit beta-1
PRO_0000000458

Regions

Topological domain26 – 245220Extracellular Probable
Transmembrane246 – 26722Helical; Probable
Transmembrane271 – 29323Helical; Probable
Transmembrane305 – 32723Helical; Probable
Topological domain328 – 451124Cytoplasmic Probable
Transmembrane452 – 47322Helical; Probable

Amino acid modifications

Glycosylation331N-linked (GlcNAc...) Potential
Glycosylation1051N-linked (GlcNAc...) Potential
Glycosylation1741N-linked (GlcNAc...) Potential
Disulfide bond161 ↔ 175 By similarity

Experimental info

Sequence conflict4201R → Q no nucleotide entry Ref.2
Sequence conflict4281R → P no nucleotide entry Ref.2

Sequences

Sequence LengthMass (Da)Tools
P15431 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: 986277FDBD66377A

FASTA47454,072
        10         20         30         40         50         60 
MWTVQNRESL GLLSFPVMVA MVCCAHSSNE PSNMSYVKET VDRLLKGYDI RLRPDFGGPP 

        70         80         90        100        110        120 
VDVGMRIDVA SIDMVSEVNM DYTLTMYFQQ SWKDKRLSYS GIPLNLTLDN RVADQLWVPD 

       130        140        150        160        170        180 
TYFLNDKKSF VHGVTVKNRM IRLHPDGTVL YGLRITTTAA CMMDLRRYPL DEQNCTLEIE 

       190        200        210        220        230        240 
SYGYTTDDIE FYWNGGEGAV TGVNKIELPQ FSIVDYKMVS KKVEFTTGAY PRLSLSFRLK 

       250        260        270        280        290        300 
RNIGYFILQT YMPSTLITIL SWVSFWINYD ASAARVALGI TTVLTMTTIS THLRETLPKI 

       310        320        330        340        350        360 
PYVKAIDIYL MGCFVFVFLA LLEYAFVNYI FFGKGPQKKG ASKQDQSANE KNKLEMNKVQ 

       370        380        390        400        410        420 
VDAHGNILLS TLEIRNETSG SEVLTGVSDP KATMYSYDSA SIQYRKPLSS REGFGRGLDR 

       430        440        450        460        470 
HGVPGKGRIR RRASQLKVKI PDLTDVNSID KWSRMFFPIT FSLFNVVYWL YYVH 

« Hide

References

[1]"GABAA receptor beta subunit heterogeneity: functional expression of cloned cDNAs."
Ymer S., Schofield P.R., Draguhn A., Werner P., Koehler M., Seeburg P.H.
EMBO J. 8:1665-1670(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"GABAA-receptor expressed from rat brain alpha- and beta-subunit cDNAs displays potentiation by benzodiazepine receptor ligands."
Malherbe P., Draguhn A., Multhaup G., Beyreuther K., Mohler H.
Brain Res. Mol. Brain Res. 8:199-208(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X15466 mRNA. Translation: CAA33493.1.
PIRB60039. S04464.
RefSeqNP_037088.1. NM_012956.1.
UniGeneRn.207157.

3D structure databases

ProteinModelPortalP15431.
SMRP15431. Positions 274-330.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP15431. 2 interactions.
MINTMINT-219840.
STRING10116.ENSRNOP00000003170.

Chemistry

BindingDBP15431.
ChEMBLCHEMBL2096683.
GuidetoPHARMACOLOGY410.

Protein family/group databases

TCDB1.A.9.5.1. the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family.

PTM databases

PhosphoSiteP15431.

Proteomic databases

PaxDbP15431.
PRIDEP15431.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID25450.
KEGGrno:25450.
UCSCRGD:2649. rat.

Organism-specific databases

CTD2560.
RGD2649. Gabrb1.

Phylogenomic databases

eggNOGNOG77438.
HOGENOMHOG000231335.
HOVERGENHBG051707.
InParanoidP15431.
KOK05181.
OrthoDBEOG712TVZ.
PhylomeDBP15431.
TreeFamTF315453.

Gene expression databases

GenevestigatorP15431.

Family and domain databases

Gene3D2.70.170.10. 1 hit.
InterProIPR006028. GABAA/Glycine_rcpt.
IPR002289. GABAAb_rcpt.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERPTHR18945. PTHR18945. 1 hit.
PfamPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSPR01160. GABAARBETA.
PR00253. GABAARECEPTR.
PR00252. NRIONCHANNEL.
SUPFAMSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsTIGR00860. LIC. 1 hit.
PROSITEPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio606697.
PROP15431.

Entry information

Entry nameGBRB1_RAT
AccessionPrimary (citable) accession number: P15431
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: June 11, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families