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Protein

ATP-dependent RNA helicase MSS116, mitochondrial

Gene

MSS116

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent RNA helicase required for mitochondrial splicing of group I and II introns. Specifically involved in the ATP-dependent splicing of the bl1 intron of COB. Also required for efficient mitochondrial translation.4 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi152 – 159ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: SGD
  • RNA binding Source: SGD
  • RNA strand annealing activity Source: SGD

GO - Biological processi

  • Group II intron splicing Source: SGD
  • Group I intron splicing Source: SGD
  • mRNA processing Source: UniProtKB-KW
  • regulation of translation Source: UniProtKB-KW
  • RNA folding Source: SGD
  • RNA secondary structure unwinding Source: GO_Central
  • transcription elongation from mitochondrial promoter Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

mRNA processing, mRNA splicing, Translation regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29781-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase MSS116, mitochondrial (EC:3.6.4.13)
Gene namesi
Name:MSS116
Ordered Locus Names:YDR194C
ORF Names:YD9346.05C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR194C.
SGDiS000002602. MSS116.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 26MitochondrionSequence analysisAdd BLAST26
ChainiPRO_000003081227 – 664ATP-dependent RNA helicase MSS116, mitochondrialAdd BLAST638

Proteomic databases

MaxQBiP15424.
PRIDEiP15424.

Interactioni

Protein-protein interaction databases

BioGridi32246. 51 interactors.
DIPiDIP-5368N.
IntActiP15424. 29 interactors.
MINTiMINT-525340.

Structurei

Secondary structure

1664
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi89 – 93Combined sources5
Helixi104 – 109Combined sources6
Helixi115 – 122Combined sources8
Turni123 – 125Combined sources3
Helixi131 – 141Combined sources11
Beta strandi142 – 151Combined sources10
Helixi158 – 172Combined sources15
Turni173 – 175Combined sources3
Beta strandi183 – 186Combined sources4
Helixi190 – 206Combined sources17
Helixi208 – 210Combined sources3
Beta strandi215 – 218Combined sources4
Helixi224 – 234Combined sources11
Beta strandi237 – 241Combined sources5
Helixi243 – 257Combined sources15
Beta strandi258 – 260Combined sources3
Beta strandi263 – 267Combined sources5
Helixi269 – 272Combined sources4
Turni275 – 277Combined sources3
Helixi278 – 291Combined sources14
Beta strandi300 – 307Combined sources8
Helixi311 – 315Combined sources5
Turni316 – 319Combined sources4
Beta strandi322 – 331Combined sources10
Beta strandi333 – 335Combined sources3
Beta strandi342 – 351Combined sources10
Helixi354 – 369Combined sources16
Turni370 – 372Combined sources3
Beta strandi375 – 379Combined sources5
Helixi383 – 397Combined sources15
Turni398 – 400Combined sources3
Beta strandi403 – 407Combined sources5
Helixi412 – 424Combined sources13
Beta strandi426 – 432Combined sources7
Helixi434 – 436Combined sources3
Beta strandi437 – 439Combined sources3
Beta strandi447 – 452Combined sources6
Beta strandi455 – 457Combined sources3
Helixi459 – 464Combined sources6
Helixi469 – 471Combined sources3
Beta strandi474 – 481Combined sources8
Helixi482 – 484Combined sources3
Helixi485 – 495Combined sources11
Beta strandi501 – 505Combined sources5
Helixi509 – 518Combined sources10
Helixi523 – 539Combined sources17
Helixi541 – 544Combined sources4
Helixi548 – 556Combined sources9
Helixi558 – 562Combined sources5
Beta strandi570 – 572Combined sources3
Helixi574 – 580Combined sources7
Beta strandi583 – 585Combined sources3
Helixi586 – 591Combined sources6
Beta strandi592 – 594Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3I5XX-ray1.90A37-597[»]
3I5YX-ray2.49A37-597[»]
3I61X-ray2.10A37-597[»]
3I62X-ray1.95A37-597[»]
3SQWX-ray1.91A88-664[»]
3SQXX-ray2.11A88-597[»]
4DB2X-ray3.16A/B/C/D342-596[»]
4DB4X-ray3.60A/B342-596[»]
4TYNX-ray2.96A88-596[»]
4TYWX-ray2.20A88-595[»]
4TYYX-ray2.74A88-596[»]
4TZ0X-ray2.35A88-596[»]
4TZ6X-ray3.21A88-596[»]
ProteinModelPortaliP15424.
SMRiP15424.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15424.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini139 – 326Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST188
Domaini355 – 512Helicase C-terminalPROSITE-ProRule annotationAdd BLAST158

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi106 – 134Q motifAdd BLAST29
Motifi267 – 270DEAD box4

Domaini

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00860000134567.
HOGENOMiHOG000113654.
InParanoidiP15424.
KOiK17679.
OMAiTPVQQKT.
OrthoDBiEOG092C128B.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15424-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLTSILIKGR TPVLASRNLL AALSNCNHIT WAVSRRLYND GNRDQRNFGR
60 70 80 90 100
NQRNNNSNRY RNSRFNSRPR TRSREDDDEV HFDKTTFSKL IHVPKEDNSK
110 120 130 140 150
EVTLDSLLEE GVLDKEIHKA ITRMEFPGLT PVQQKTIKPI LSSEDHDVIA
160 170 180 190 200
RAKTGTGKTF AFLIPIFQHL INTKFDSQYM VKAVIVAPTR DLALQIEAEV
210 220 230 240 250
KKIHDMNYGL KKYACVSLVG GTDFRAAMNK MNKLRPNIVI ATPGRLIDVL
260 270 280 290 300
EKYSNKFFRF VDYKVLDEAD RLLEIGFRDD LETISGILNE KNSKSADNIK
310 320 330 340 350
TLLFSATLDD KVQKLANNIM NKKECLFLDT VDKNEPEAHE RIDQSVVISE
360 370 380 390 400
KFANSIFAAV EHIKKQIKER DSNYKAIIFA PTVKFTSFLC SILKNEFKKD
410 420 430 440 450
LPILEFHGKI TQNKRTSLVK RFKKDESGIL VCTDVGARGM DFPNVHEVLQ
460 470 480 490 500
IGVPSELANY IHRIGRTARS GKEGSSVLFI CKDELPFVRE LEDAKNIVIA
510 520 530 540 550
KQEKYEPSEE IKSEVLEAVT EEPEDISDIV ISLISSYRSC IKEYRFSERR
560 570 580 590 600
ILPEIASTYG VLLNDPQLKI PVSRRFLDKL GLSRSPIGKA MFEIRDYSSR
610 620 630 640 650
DGNNKSYDYD DDSEISFRGN KNYNNRSQNR DYDDEPFRRS NNNRRSFSRS
660
NDKNNYSSRN SNIY
Length:664
Mass (Da):76,269
Last modified:April 1, 1990 - v1
Checksum:iDAEECCA4ED0CB2BE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48784 Genomic DNA. Translation: CAA88707.1.
BK006938 Genomic DNA. Translation: DAA12036.1.
PIRiS02116.
RefSeqiNP_010480.1. NM_001180502.1.

Genome annotation databases

EnsemblFungiiYDR194C; YDR194C; YDR194C.
GeneIDi851775.
KEGGisce:YDR194C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48784 Genomic DNA. Translation: CAA88707.1.
BK006938 Genomic DNA. Translation: DAA12036.1.
PIRiS02116.
RefSeqiNP_010480.1. NM_001180502.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3I5XX-ray1.90A37-597[»]
3I5YX-ray2.49A37-597[»]
3I61X-ray2.10A37-597[»]
3I62X-ray1.95A37-597[»]
3SQWX-ray1.91A88-664[»]
3SQXX-ray2.11A88-597[»]
4DB2X-ray3.16A/B/C/D342-596[»]
4DB4X-ray3.60A/B342-596[»]
4TYNX-ray2.96A88-596[»]
4TYWX-ray2.20A88-595[»]
4TYYX-ray2.74A88-596[»]
4TZ0X-ray2.35A88-596[»]
4TZ6X-ray3.21A88-596[»]
ProteinModelPortaliP15424.
SMRiP15424.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32246. 51 interactors.
DIPiDIP-5368N.
IntActiP15424. 29 interactors.
MINTiMINT-525340.

Proteomic databases

MaxQBiP15424.
PRIDEiP15424.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR194C; YDR194C; YDR194C.
GeneIDi851775.
KEGGisce:YDR194C.

Organism-specific databases

EuPathDBiFungiDB:YDR194C.
SGDiS000002602. MSS116.

Phylogenomic databases

GeneTreeiENSGT00860000134567.
HOGENOMiHOG000113654.
InParanoidiP15424.
KOiK17679.
OMAiTPVQQKT.
OrthoDBiEOG092C128B.

Enzyme and pathway databases

BioCyciYEAST:G3O-29781-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP15424.
PROiP15424.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMS116_YEAST
AccessioniPrimary (citable) accession number: P15424
Secondary accession number(s): D6VSH6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: November 30, 2016
This is version 164 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 10300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.