P15399 (PBAS_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probasin
Short name=PB

Alternative name(s):
M-40

Gene names

Name:	Pbsn
Synonyms:	Prbs

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	177 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Nucleus. Secreted.
Tissue specificity	Prostatic epithelial cells.
Induction	Androgen-regulated, increases in concentration with zinc uptake.
Sequence similarities	Belongs to the calycin superfamily. Lipocalin family.

Ontologies

Keywords
Cellular component	Nucleus Secreted
Coding sequence diversity	Alternative initiation
Domain	Signal
PTM	Disulfide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	odorant binding Inferred from electronic annotation. Source: InterPro transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform 1 (identifier: P15399-1)

Also known as: Secreted;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P15399-2)

Also known as: Nuclear;

The sequence of this isoform differs from the canonical sequence as follows:
     1-17: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Ref.1
	Chain	18 – 177	160	Probasin		PRO_0000017955
Amino acid modifications
	Disulfide bond	79 ↔ 170		By similarity
Natural variations
	Alternative sequence	1 – 17	17	Missing in isoform 2.		VSP_018809

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Secreted) [UniParc]. Last modified April 1, 1990. Version 1. Checksum: 1805977EA136669A	FASTA	177	20,780
	10 20 30 40 50 60 MRVILLLLTL DVLGVSSMMT DKNLKKKIEG NWRTVYLAAS SVEKINEGSP LRTYFRRIEC 70 80 90 100 110 120 GKRCNRINLY FYIKKGAKCQ QFKIVGRRSQ DVYYAKYEGS TAFMLKTVNE KILLFDYFNR 130 140 150 160 170 NRRNDVTRVA GVLAKGRQLT KDEMTEYMNF VEEMGIEDEN VQRVMDTDTC PNKIRIR
	Isoform 2 (Nuclear) [UniParc]. Checksum: 3741E7446DBCF3F3	FASTA	160	18,956
	10 20 30 40 50 60 MMTDKNLKKK IEGNWRTVYL AASSVEKINE GSPLRTYFRR IECGKRCNRI NLYFYIKKGA 70 80 90 100 110 120 KCQQFKIVGR RSQDVYYAKY EGSTAFMLKT VNEKILLFDY FNRNRRNDVT RVAGVLAKGR 130 140 150 160 QLTKDEMTEY MNFVEEMGIE DENVQRVMDT DTCPNKIRIR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Regulation of a bifunctional mRNA results in synthesis of secreted and nuclear probasin." Spence A.M., Sheppard P.C., Davie J.R., Matuo Y., Nishi N., McKeehan W.L., Dodd J.G., Matusik R.J. Proc. Natl. Acad. Sci. U.S.A. 86:7843-7847(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), PROTEIN SEQUENCE OF 18-40.
[2]	"Rat probasin: structure and function of an outlier lipocalin." Kasper S., Matusik R.J. Biochim. Biophys. Acta 1482:249-258(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Prostate.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M27156 mRNA. Translation: AAA40805.1. AY370611 Genomic DNA. Translation: AAQ67415.1. BC062035 mRNA. Translation: AAH62035.1.
IPI	IPI00208410. IPI00760153.
PIR	A32602.
RefSeq	NP_061998.1. NM_019125.1.
UniGene	Rn.9862.
3D structure databases
ProteinModelPortal	P15399.
ModBase	Search...
Protein-protein interaction databases
IntAct	P15399. 1 interaction.
STRING	10116.ENSRNOP00000042730.
Proteomic databases
PRIDE	P15399.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000046768; ENSRNOP00000042730; ENSRNOG00000003713.
GeneID	54193.
KEGG	rno:54193.
Organism-specific databases
CTD	54192.
RGD	708571. Pbsn.
Phylogenomic databases
GeneTree	ENSGT00530000063356.
HOGENOM	HOG000049215.
HOVERGEN	HBG098951.
InParanoid	P15399.
OMA	GECVENS.
OrthoDB	EOG4N04G8.
Gene expression databases
Genevestigator	P15399.
GermOnline	ENSRNOG00000003713. Rattus norvegicus.
Family and domain databases
Gene3D	2.40.128.20. 1 hit.
InterPro	IPR012674. Calycin. IPR011038. Calycin-like. IPR022272. Lipocalin_CS. IPR000566. Lipocln_cytosolic_FA-bd_dom. IPR002448. Odour-bd. [Graphical view]
Pfam	PF00061. Lipocalin. 1 hit. [Graphical view]
PRINTS	PR01173. ODORANTBNDNG.
SUPFAM	SSF50814. Calycin. 1 hit.
PROSITE	PS00213. LIPOCALIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	610634.

Entry information

Entry name

PBAS_RAT

Accession

Primary (citable) accession number: P15399
Secondary accession number(s): Q53Z30

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	April 3, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program