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P15396

- ENPP3_BOVIN

UniProt

P15396 - ENPP3_BOVIN

Protein

Ectonucleotide pyrophosphatase/phosphodiesterase family member 3

Gene

ENPP3

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 2 (20 Mar 2007)
      Previous versions | rss
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    Functioni

    Cleaves a variety of phosphodiester and phosphosulfate bonds including deoxynucleotides, nucleotide sugars, and NAD.By similarity

    Catalytic activityi

    Hydrolytically removes 5'-nucleotides successively from the 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides.
    A dinucleotide + H2O = 2 mononucleotides.

    Cofactori

    Binds 2 divalent metal cations per subunit.Curated

    Enzyme regulationi

    At low concentrations of ATP, a phosphorylated active site intermediate is formed which inhibits further ATP hydrolysis.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi167 – 1671Zinc 1; catalyticBy similarity
    Active sitei205 – 2051AMP-threonine intermediate1 Publication
    Metal bindingi205 – 2051Zinc 1; catalyticBy similarity
    Binding sitei226 – 2261SubstrateBy similarity
    Binding sitei320 – 3201SubstrateBy similarity
    Metal bindingi325 – 3251Zinc 2; catalyticBy similarity
    Metal bindingi329 – 3291Zinc 2; via tele nitrogen; catalyticBy similarity
    Metal bindingi372 – 3721Zinc 1; catalyticBy similarity
    Metal bindingi373 – 3731Zinc 1; via tele nitrogen; catalyticBy similarity
    Metal bindingi482 – 4821Zinc 2; via tele nitrogen; catalyticBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. NADH pyrophosphatase activity Source: UniProtKB-EC
    3. nucleic acid binding Source: InterPro
    4. phosphodiesterase I activity Source: UniProtKB-EC
    5. polysaccharide binding Source: InterPro
    6. scavenger receptor activity Source: InterPro

    GO - Biological processi

    1. immune response Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    SABIO-RKP15396.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ectonucleotide pyrophosphatase/phosphodiesterase family member 3
    Short name:
    E-NPP 3
    Alternative name(s):
    Phosphodiesterase I beta
    Short name:
    PD-Ibeta
    Phosphodiesterase I/nucleotide pyrophosphatase 3
    CD_antigen: CD203c
    Including the following 2 domains:
    Alkaline phosphodiesterase I (EC:3.1.4.1)
    Nucleotide pyrophosphatase (EC:3.6.1.9)
    Short name:
    NPPase
    Gene namesi
    Name:ENPP3
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    Membrane Curated; Single-pass type II membrane protein Curated. Secreted By similarity
    Note: Located to the apical surface in intestinal and kidney epithelial cells. Located to the cell surface of basophils, and to the apical plasma membrane of bile duct cells. Secreted in serum, and in lumen of epithelial cells By similarity.By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 874874Ectonucleotide pyrophosphatase/phosphodiesterase family member 3PRO_0000058534Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi54 ↔ 71PROSITE-ProRule annotation
    Disulfide bondi58 ↔ 89PROSITE-ProRule annotation
    Disulfide bondi69 ↔ 82PROSITE-ProRule annotation
    Disulfide bondi75 ↔ 81PROSITE-ProRule annotation
    Disulfide bondi98 ↔ 115PROSITE-ProRule annotation
    Disulfide bondi103 ↔ 133PROSITE-ProRule annotation
    Disulfide bondi113 ↔ 126PROSITE-ProRule annotation
    Disulfide bondi119 ↔ 125PROSITE-ProRule annotation
    Disulfide bondi144 ↔ 190PROSITE-ProRule annotation
    Disulfide bondi152 ↔ 364PROSITE-ProRule annotation
    Glycosylationi236 – 2361N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi279 – 2791N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi290 – 2901N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi380 ↔ 477PROSITE-ProRule annotation
    Glycosylationi425 – 4251N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi428 ↔ 817PROSITE-ProRule annotation
    Glycosylationi532 – 5321N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi574 ↔ 678PROSITE-ProRule annotation
    Disulfide bondi576 ↔ 663PROSITE-ProRule annotation
    Glycosylationi677 – 6771N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi686 – 6861N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi698 – 6981N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi770 – 7701N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi786 ↔ 796PROSITE-ProRule annotation
    Glycosylationi788 – 7881N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi820 – 8201N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    N-glycosylation is necessary for correct trafficking to the apical surface, but is not the apical targeting signal.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiP15396.

    Interactioni

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000026900.

    Structurei

    3D structure databases

    ProteinModelPortaliP15396.
    SMRiP15396. Positions 96-138.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1111CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini31 – 874844ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei12 – 3019Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini50 – 9344SMB 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini94 – 13845SMB 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni140 – 509370PhosphodiesteraseAdd
    BLAST
    Regioni604 – 874271NucleaseAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi78 – 803Cell attachment siteSequence Analysis

    Sequence similaritiesi

    Contains 2 SMB (somatomedin-B) domains.Curated

    Keywords - Domaini

    Repeat, Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1524.
    HOGENOMiHOG000037439.
    HOVERGENiHBG051484.
    InParanoidiP15396.
    KOiK01513.

    Family and domain databases

    Gene3Di3.40.570.10. 1 hit.
    3.40.720.10. 1 hit.
    InterProiIPR017849. Alkaline_Pase-like_a/b/a.
    IPR017850. Alkaline_phosphatase_core.
    IPR001604. DNA/RNA_non-sp_Endonuclease.
    IPR024873. E-NPP.
    IPR020821. Extracellular_endonuc_su_A.
    IPR002591. Phosphodiest/P_Trfase.
    IPR001212. Somatomedin_B_dom.
    [Graphical view]
    PANTHERiPTHR10151. PTHR10151. 1 hit.
    PfamiPF01223. Endonuclease_NS. 1 hit.
    PF01663. Phosphodiest. 1 hit.
    PF01033. Somatomedin_B. 2 hits.
    [Graphical view]
    SMARTiSM00892. Endonuclease_NS. 1 hit.
    SM00477. NUC. 1 hit.
    SM00201. SO. 2 hits.
    [Graphical view]
    SUPFAMiSSF53649. SSF53649. 1 hit.
    PROSITEiPS00524. SMB_1. 2 hits.
    PS50958. SMB_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P15396-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQSTLNLSTE EPVKRNTVKK YKIICIVLLI LLVAVSLALG LVAGLRQQEE    50
    QGSCRKKCFD ASHRGLEGCR CDVGCKGRGD CCWDFEDTCV QSTQIWTCNK 100
    FRCGETRLES SLCSCSDDCL QRKDCCADYK SVCQGETSWV DEDCSTAQQP 150
    QCPEGFDLPP VILFSMDGFR AEYLQTWSTL VPNINKLKTC GVHSQYLRPA 200
    YPTKTFPNHY TIVTGLYPES HGIIDNNMYD INLNKNFSLS SKEKDNPAWW 250
    QGQPIWLTAM YQGLKVGTYF WPGSDVAING TFPSIYKIYN RSVTYEERIF 300
    TLLKWLDLPK AERPDFYTIY VEEPDSQGHN YGPVSAGVIQ ALQLVDKTFG 350
    LLMEGLKQRN LVNCVNIILL ADHGMDQTYC DKLEYMADYF SSINFYMFEG 400
    PAPRIRTRNI PQDFFTFNSE EIVRNLSCRK PDQHFKPYLS PDLPKRLHFA 450
    KNVRIDKVNL LVDRQWQAVR NRAYSYCGGG NHGYDNEFKS MEAIFLAHGP 500
    SFKQKTEVEP FDNIEVYNLL CDLLHIQPAP NNGTHGSLNH LLKVPFYEPS 550
    HAEELSKFSV CGFTVPLPTD TLGCSCSRLQ TNSDLERVNQ MLDLTQDEIT 600
    ATEKLNLPFG RPRLIQKNKE PCLLYHREYV SGFDKTLRMP LWSSYTVPKP 650
    GDTSPLPPTV PDCLRADVRV APSESQNCSF SLADKNITHG FLYPPANNRT 700
    SNSQYDALIT SNLVPMYEAF KTMWNYFHSV LLVKYAMERN GVNVVSGPVF 750
    DYDYDGHFDA PDEIADYAVN TSVPIPTHYF VVLTSCKNQS QTPDACTGWL 800
    DVLPFVIPHR PTNVESCPEN KSESLWVEER FNVHTARVRD VELLTGLDFY 850
    QEKAQPVSEI LQLKTYLPVF ETVI 874
    Length:874
    Mass (Da):99,523
    Last modified:March 20, 2007 - v2
    Checksum:iA901CC5B7E787F40
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC122742 mRNA. Translation: AAI22743.1.
    PIRiA25274.
    RefSeqiNP_001069391.1. NM_001075923.2.
    UniGeneiBt.9659.

    Genome annotation databases

    GeneIDi529405.
    KEGGibta:529405.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC122742 mRNA. Translation: AAI22743.1 .
    PIRi A25274.
    RefSeqi NP_001069391.1. NM_001075923.2.
    UniGenei Bt.9659.

    3D structure databases

    ProteinModelPortali P15396.
    SMRi P15396. Positions 96-138.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000026900.

    Proteomic databases

    PRIDEi P15396.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 529405.
    KEGGi bta:529405.

    Organism-specific databases

    CTDi 5169.

    Phylogenomic databases

    eggNOGi COG1524.
    HOGENOMi HOG000037439.
    HOVERGENi HBG051484.
    InParanoidi P15396.
    KOi K01513.

    Enzyme and pathway databases

    SABIO-RK P15396.

    Miscellaneous databases

    NextBioi 20875027.

    Family and domain databases

    Gene3Di 3.40.570.10. 1 hit.
    3.40.720.10. 1 hit.
    InterProi IPR017849. Alkaline_Pase-like_a/b/a.
    IPR017850. Alkaline_phosphatase_core.
    IPR001604. DNA/RNA_non-sp_Endonuclease.
    IPR024873. E-NPP.
    IPR020821. Extracellular_endonuc_su_A.
    IPR002591. Phosphodiest/P_Trfase.
    IPR001212. Somatomedin_B_dom.
    [Graphical view ]
    PANTHERi PTHR10151. PTHR10151. 1 hit.
    Pfami PF01223. Endonuclease_NS. 1 hit.
    PF01663. Phosphodiest. 1 hit.
    PF01033. Somatomedin_B. 2 hits.
    [Graphical view ]
    SMARTi SM00892. Endonuclease_NS. 1 hit.
    SM00477. NUC. 1 hit.
    SM00201. SO. 2 hits.
    [Graphical view ]
    SUPFAMi SSF53649. SSF53649. 1 hit.
    PROSITEi PS00524. SMB_1. 2 hits.
    PS50958. SMB_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Crossbred X Angus.
      Tissue: Ileum.
    2. "Amino acid sequence of the active site peptide of bovine intestinal 5'-nucleotide phosphodiesterase and identification of the active site residue as threonine."
      Culp J.S., Blytt H.J., Hermodson M., Butler L.G.
      J. Biol. Chem. 260:8320-8324(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 167-226, ACTIVE SITE THR-205.
      Tissue: Intestine.

    Entry informationi

    Entry nameiENPP3_BOVIN
    AccessioniPrimary (citable) accession number: P15396
    Secondary accession number(s): Q0II99
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: March 20, 2007
    Last modified: October 1, 2014
    This is version 93 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3