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P15396

- ENPP3_BOVIN

UniProt

P15396 - ENPP3_BOVIN

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Protein
Ectonucleotide pyrophosphatase/phosphodiesterase family member 3
Gene
ENPP3
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Cleaves a variety of phosphodiester and phosphosulfate bonds including deoxynucleotides, nucleotide sugars, and NAD By similarity.

Catalytic activityi

Hydrolytically removes 5'-nucleotides successively from the 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides.
A dinucleotide + H2O = 2 mononucleotides.

Cofactori

Binds 2 divalent metal cations per subunit Inferred.

Enzyme regulationi

At low concentrations of ATP, a phosphorylated active site intermediate is formed which inhibits further ATP hydrolysis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi167 – 1671Zinc 1; catalytic By similarity
Active sitei205 – 2051AMP-threonine intermediate1 Publication
Metal bindingi205 – 2051Zinc 1; catalytic By similarity
Binding sitei226 – 2261Substrate By similarity
Binding sitei320 – 3201Substrate By similarity
Metal bindingi325 – 3251Zinc 2; catalytic By similarity
Metal bindingi329 – 3291Zinc 2; via tele nitrogen; catalytic By similarity
Metal bindingi372 – 3721Zinc 1; catalytic By similarity
Metal bindingi373 – 3731Zinc 1; via tele nitrogen; catalytic By similarity
Metal bindingi482 – 4821Zinc 2; via tele nitrogen; catalytic By similarity

GO - Molecular functioni

  1. NADH pyrophosphatase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
  3. nucleic acid binding Source: InterPro
  4. phosphodiesterase I activity Source: UniProtKB-EC
  5. polysaccharide binding Source: InterPro
  6. scavenger receptor activity Source: InterPro

GO - Biological processi

  1. immune response Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

SABIO-RKP15396.

Names & Taxonomyi

Protein namesi
Recommended name:
Ectonucleotide pyrophosphatase/phosphodiesterase family member 3
Short name:
E-NPP 3
Alternative name(s):
Phosphodiesterase I beta
Short name:
PD-Ibeta
Phosphodiesterase I/nucleotide pyrophosphatase 3
CD_antigen: CD203c
Including the following 2 domains:
Alkaline phosphodiesterase I (EC:3.1.4.1)
Nucleotide pyrophosphatase (EC:3.6.1.9)
Short name:
NPPase
Gene namesi
Name:ENPP3
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

Membrane; Single-pass type II membrane protein Reviewed prediction. Secreted By similarity
Note: Located to the apical surface in intestinal and kidney epithelial cells. Located to the cell surface of basophils, and to the apical plasma membrane of bile duct cells. Secreted in serum, and in lumen of epithelial cells By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1111Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei12 – 3019Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST
Topological domaini31 – 874844Extracellular Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 874874Ectonucleotide pyrophosphatase/phosphodiesterase family member 3
PRO_0000058534Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi54 ↔ 71 By similarity
Disulfide bondi58 ↔ 89 By similarity
Disulfide bondi69 ↔ 82 By similarity
Disulfide bondi75 ↔ 81 By similarity
Disulfide bondi98 ↔ 115 By similarity
Disulfide bondi103 ↔ 133 By similarity
Disulfide bondi113 ↔ 126 By similarity
Disulfide bondi119 ↔ 125 By similarity
Disulfide bondi144 ↔ 190 By similarity
Disulfide bondi152 ↔ 364 By similarity
Glycosylationi236 – 2361N-linked (GlcNAc...) Reviewed prediction
Glycosylationi279 – 2791N-linked (GlcNAc...) Reviewed prediction
Glycosylationi290 – 2901N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi380 ↔ 477 By similarity
Glycosylationi425 – 4251N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi428 ↔ 817 By similarity
Glycosylationi532 – 5321N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi574 ↔ 678 By similarity
Disulfide bondi576 ↔ 663 By similarity
Glycosylationi677 – 6771N-linked (GlcNAc...) Reviewed prediction
Glycosylationi686 – 6861N-linked (GlcNAc...) Reviewed prediction
Glycosylationi698 – 6981N-linked (GlcNAc...) Reviewed prediction
Glycosylationi770 – 7701N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi786 ↔ 796 By similarity
Glycosylationi788 – 7881N-linked (GlcNAc...) Reviewed prediction
Glycosylationi820 – 8201N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

N-glycosylation is necessary for correct trafficking to the apical surface, but is not the apical targeting signal By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP15396.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000026900.

Structurei

3D structure databases

ProteinModelPortaliP15396.
SMRiP15396. Positions 96-138.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini50 – 9344SMB 1
Add
BLAST
Domaini94 – 13845SMB 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni140 – 509370Phosphodiesterase
Add
BLAST
Regioni604 – 874271Nuclease
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi78 – 803Cell attachment site Reviewed prediction

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1524.
HOGENOMiHOG000037439.
HOVERGENiHBG051484.
InParanoidiP15396.
KOiK01513.

Family and domain databases

Gene3Di3.40.570.10. 1 hit.
3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR001604. DNA/RNA_non-sp_Endonuclease.
IPR024873. E-NPP.
IPR020821. Extracellular_endonuc_su_A.
IPR002591. Phosphodiest/P_Trfase.
IPR001212. Somatomedin_B_dom.
[Graphical view]
PANTHERiPTHR10151. PTHR10151. 1 hit.
PfamiPF01223. Endonuclease_NS. 1 hit.
PF01663. Phosphodiest. 1 hit.
PF01033. Somatomedin_B. 2 hits.
[Graphical view]
SMARTiSM00892. Endonuclease_NS. 1 hit.
SM00477. NUC. 1 hit.
SM00201. SO. 2 hits.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00524. SMB_1. 2 hits.
PS50958. SMB_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P15396-1 [UniParc]FASTAAdd to Basket

« Hide

MQSTLNLSTE EPVKRNTVKK YKIICIVLLI LLVAVSLALG LVAGLRQQEE    50
QGSCRKKCFD ASHRGLEGCR CDVGCKGRGD CCWDFEDTCV QSTQIWTCNK 100
FRCGETRLES SLCSCSDDCL QRKDCCADYK SVCQGETSWV DEDCSTAQQP 150
QCPEGFDLPP VILFSMDGFR AEYLQTWSTL VPNINKLKTC GVHSQYLRPA 200
YPTKTFPNHY TIVTGLYPES HGIIDNNMYD INLNKNFSLS SKEKDNPAWW 250
QGQPIWLTAM YQGLKVGTYF WPGSDVAING TFPSIYKIYN RSVTYEERIF 300
TLLKWLDLPK AERPDFYTIY VEEPDSQGHN YGPVSAGVIQ ALQLVDKTFG 350
LLMEGLKQRN LVNCVNIILL ADHGMDQTYC DKLEYMADYF SSINFYMFEG 400
PAPRIRTRNI PQDFFTFNSE EIVRNLSCRK PDQHFKPYLS PDLPKRLHFA 450
KNVRIDKVNL LVDRQWQAVR NRAYSYCGGG NHGYDNEFKS MEAIFLAHGP 500
SFKQKTEVEP FDNIEVYNLL CDLLHIQPAP NNGTHGSLNH LLKVPFYEPS 550
HAEELSKFSV CGFTVPLPTD TLGCSCSRLQ TNSDLERVNQ MLDLTQDEIT 600
ATEKLNLPFG RPRLIQKNKE PCLLYHREYV SGFDKTLRMP LWSSYTVPKP 650
GDTSPLPPTV PDCLRADVRV APSESQNCSF SLADKNITHG FLYPPANNRT 700
SNSQYDALIT SNLVPMYEAF KTMWNYFHSV LLVKYAMERN GVNVVSGPVF 750
DYDYDGHFDA PDEIADYAVN TSVPIPTHYF VVLTSCKNQS QTPDACTGWL 800
DVLPFVIPHR PTNVESCPEN KSESLWVEER FNVHTARVRD VELLTGLDFY 850
QEKAQPVSEI LQLKTYLPVF ETVI 874
Length:874
Mass (Da):99,523
Last modified:March 20, 2007 - v2
Checksum:iA901CC5B7E787F40
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC122742 mRNA. Translation: AAI22743.1.
PIRiA25274.
RefSeqiNP_001069391.1. NM_001075923.2.
UniGeneiBt.9659.

Genome annotation databases

GeneIDi529405.
KEGGibta:529405.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC122742 mRNA. Translation: AAI22743.1 .
PIRi A25274.
RefSeqi NP_001069391.1. NM_001075923.2.
UniGenei Bt.9659.

3D structure databases

ProteinModelPortali P15396.
SMRi P15396. Positions 96-138.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000026900.

Proteomic databases

PRIDEi P15396.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 529405.
KEGGi bta:529405.

Organism-specific databases

CTDi 5169.

Phylogenomic databases

eggNOGi COG1524.
HOGENOMi HOG000037439.
HOVERGENi HBG051484.
InParanoidi P15396.
KOi K01513.

Enzyme and pathway databases

SABIO-RK P15396.

Miscellaneous databases

NextBioi 20875027.

Family and domain databases

Gene3Di 3.40.570.10. 1 hit.
3.40.720.10. 1 hit.
InterProi IPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR001604. DNA/RNA_non-sp_Endonuclease.
IPR024873. E-NPP.
IPR020821. Extracellular_endonuc_su_A.
IPR002591. Phosphodiest/P_Trfase.
IPR001212. Somatomedin_B_dom.
[Graphical view ]
PANTHERi PTHR10151. PTHR10151. 1 hit.
Pfami PF01223. Endonuclease_NS. 1 hit.
PF01663. Phosphodiest. 1 hit.
PF01033. Somatomedin_B. 2 hits.
[Graphical view ]
SMARTi SM00892. Endonuclease_NS. 1 hit.
SM00477. NUC. 1 hit.
SM00201. SO. 2 hits.
[Graphical view ]
SUPFAMi SSF53649. SSF53649. 1 hit.
PROSITEi PS00524. SMB_1. 2 hits.
PS50958. SMB_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Ileum.
  2. "Amino acid sequence of the active site peptide of bovine intestinal 5'-nucleotide phosphodiesterase and identification of the active site residue as threonine."
    Culp J.S., Blytt H.J., Hermodson M., Butler L.G.
    J. Biol. Chem. 260:8320-8324(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 167-226, ACTIVE SITE THR-205.
    Tissue: Intestine.

Entry informationi

Entry nameiENPP3_BOVIN
AccessioniPrimary (citable) accession number: P15396
Secondary accession number(s): Q0II99
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: March 20, 2007
Last modified: April 16, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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