P15395 (TRPE_RHIME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Anthranilate synthase
EC=4.1.3.27

Including the following 1 domains:

1. Glutamine amidotransferase

Gene names

Name:	trpE(G)
Ordered Locus Names:	R02387
ORF Names:	SMc02725

Organism

Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

266834 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium › Rhizobium meliloti

Protein attributes

Sequence length	729 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Catalytic activity	Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Domain	Glutamine amidotransferase
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW tryptophan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	anthranilate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 729	729	Anthranilate synthase		PRO_0000056868
Regions
	Domain	530 – 726	197	Glutamine amidotransferase type-1
Sites
	Active site	607	1	For GATase activity By similarity
	Active site	697	1	For GATase activity By similarity
	Active site	699	1	For GATase activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	P15395 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: F16F10A90E3846E1	FASTA	729	80,505
	10 20 30 40 50 60 MAAVILEDGA ESYTTKGGIV VTRRRREASY SDAIAGYVDR LDERRGAVFS SNYEYPGRYT 70 80 90 100 110 120 RWDTAVVDPP LAISSFGRSL WIEAYNERGE VLLALIAEDL KSVADITLGS LAARRLDLTI 130 140 150 160 170 180 NEPDRVFTEE ERSKMPTVFT VLRAVTNLFH SEEDSNLGLY GAFGYDLAFQ FDAIELKLSR 190 200 210 220 230 240 PDDQRDMVLF LPDEILVVDH YAAKAWIDRY DFARENLSTE GKAADIAPEP FRSVDSIPPH 250 260 270 280 290 300 GDHRPGEYAE LVVKAKESFR RGDLFEVVPG QKFYERCESR PSEISNRLKA INPSPYSFFI 310 320 330 340 350 360 NLGNQEYLVG ASPEMFVRVS GRRIETCPIS GTIKRGDDPI ADSEQILKLL NSKKDESELT 370 380 390 400 410 420 MCSDVDRNDK SRVCVPGSVK VIGRRQIEMY SRLIHTVDHI EGRLRDDMDA FDGFLSHAWA 430 440 450 460 470 480 VTVTGAPKLW AMRFIESHEK SPRAWYGGAI GMVGFNGDMN TGLTLRTIRI KDGIAEVRAG 490 500 510 520 530 540 ATLLYDSNPE EEEAETELKA SAMIAAIRDA KSANSAKSAR DVAAVGAGVS ILLVDHEDSF 550 560 570 580 590 600 VHTLANYFRQ TGASVTTVRT PVAEEIFDRV KPDLVVLSPG PGTPKDFDCK ATIKKARARD 610 620 630 640 650 660 LPIFGVCLGL QALAEAYGGD LRQLAIPMHG KPSRIRVLEP GIVFSGLGKE VTVGRYHSIF 670 680 690 700 710 720 ADPSNLPREF VITAESEDGT IMGIEHSKEP VAAVQFHPES IMTLGGDAGM RMIENVVAHL AKRAKTKAA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rhizobium meliloti anthranilate synthase gene: cloning, sequence, and expression in Escherichia coli." Bae Y.M., Holmgren E., Crawford I.P. J. Bacteriol. 171:3471-3478(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.
[3]	"The composite genome of the legume symbiont Sinorhizobium meliloti." Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F., Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G., Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P., Cowie A. , Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S., Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I., Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S., Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C., Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R., Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H., Wong K., Yeh K.-C., Batut J. Science 293:668-672(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M22983 Genomic DNA. Translation: AAA26370.1. AL591688 Genomic DNA. Translation: CAC46966.1.
PIR	A30904. B44515.
RefSeq	NP_386493.1. NC_003047.1.
3D structure databases
ProteinModelPortal	P15395.
ModBase	Search...
Protein-protein interaction databases
STRING	266834.SMc02725.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAC46966; CAC46966; SMc02725.
GeneID	1234054.
KEGG	sme:SMc02725.
PATRIC	23634267. VBISinMel96828_3871.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0147.
HOGENOM	HOG000271519.
KO	K13503.
OMA	GRYHSLY.
ProtClustDB	PRK13566.
Enzyme and pathway databases
BioCyc	SMEL266834:GJF6-2442-MONOMER.
UniPathway	UPA00035; UER00040.
Family and domain databases
Gene3D	3.60.120.10. 1 hit.
InterPro	IPR005801. ADC_synthase. IPR006805. Anth_synth_I_N. IPR010112. Anthranilate_synth_clad3. IPR015890. Chorismate-bd_C. IPR017926. GATASE_1. IPR006221. TrpG/PapA. [Graphical view]
Pfam	PF04715. Anth_synt_I_N. 1 hit. PF00425. Chorismate_bind. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PIRSF	PIRSF036934. TrpE-G. 1 hit.
SUPFAM	SSF56322. TRPE_1_chor_bd. 1 hit.
TIGRFAMs	TIGR01815. TrpE-clade3. 1 hit. TIGR00566. trpG_papA. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRPE_RHIME

Accession

Primary (citable) accession number: P15395

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	May 1, 2013
This is version 111 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program