P15393 (C11B1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 11B1, mitochondrial

Alternative name(s):
CYPXIB1
Cytochrome P450(11 beta)-DS
Cytochrome P450C11
Steroid 11-beta-hydroxylase
EC=1.14.15.4

Gene names

Name:	Cyp11b1
Synonyms:	Cyp11b-1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Forms corticosterone from 11-deoxycorticosterone.
Catalytic activity	A steroid + reduced adrenal ferredoxin + O2 = an 11-beta-hydroxysteroid + oxidized adrenal ferredoxin + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Mitochondrion membrane.
Tissue specificity	Adrenal zona fasciculata/reticularis.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Steroidogenesis
Cellular component	Membrane Mitochondrion
Domain	Transit peptide
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	adenosine metabolic process Inferred from expression pattern PubMed 20937274. Source: RGD aldosterone biosynthetic process Inferred from mutant phenotype PubMed 19342457. Source: UniProtKB cellular response to interferon-gamma Inferred from expression pattern PubMed 12121434. Source: RGD cortisol biosynthetic process Inferred from mutant phenotype PubMed 19342457. Source: UniProtKB dibenzo-p-dioxin metabolic process Inferred from expression pattern PubMed 16254025. Source: RGD response to dexamethasone stimulus Inferred from expression pattern PubMed 18252953. Source: RGD response to drug Inferred from expression pattern PubMed 19733641. Source: RGD
Cellular_component	integral to endoplasmic reticulum membrane Inferred from direct assay PubMed 11796518. Source: RGD mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from direct assay PubMed 8674848. Source: RGD
Molecular_function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro steroid 11-beta-monooxygenase activity Inferred from direct assay PubMed 11796518PubMed 16405651. Source: RGD steroid binding Inferred from direct assay PubMed 16405651. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 24	24	Mitochondrion Ref.6
	Chain	25 – 499	475	Cytochrome P450 11B1, mitochondrial		PRO_0000003603
Sites
	Metal binding	446	1	Iron (heme axial ligand) By similarity
Natural variations
	Natural variant	127	1	R → C in strain: Dahl salt-resistant.
	Natural variant	351	1	V → A in strain: Dahl salt-resistant.
	Natural variant	381	1	V → L in strain: Dahl salt-resistant.
	Natural variant	384	1	I → L in strain: Dahl salt-resistant.
	Natural variant	443	1	V → M in strain: Dahl salt-resistant.

Sequences

	Sequence		Length	Mass (Da)
	P15393 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: 19C041F96A673C7E	FASTA	499	57,459
	10 20 30 40 50 60 MALRVTADVW LARPWQCLHR TRALGTTAKV APKTLKPFEA IPQYSRNKWL KMIQILREQG 70 80 90 100 110 120 QENLHLEMHQ AFQELGPIFR HSAGGAQIVS VMLPEDAEKL HQVESILPHR MPLEPWVAHR 130 140 150 160 170 180 ELRGLRRGVF LLNGADWRFN RLQLNPNMLS PKAIQSFVPF VDVVARDFVE NLKKRMLENV 190 200 210 220 230 240 HGSMSINIQS NMFNYTMEAS HFVISGERLG LTGHDLKPES VTFTHALHSM FKSTTQLMFL 250 260 270 280 290 300 PKSLTRWTST RVWKEHFDSW DIISEYVTKC IKNVYRELAE GRQQSWSVIS EMVAQSTLSM 310 320 330 340 350 360 DAIHANSMEL IAGSVDTTAI SLVMTLFELA RNPDVQQALR QESLAAEASI VANPQKAMSD 370 380 390 400 410 420 LPLLRAALKE TLRLYPVGSF VERIVHSDLV LQNYHVPAGT FVIIYLYSMG RNPAVFPRPE 430 440 450 460 470 480 RYMPQRWLER KRSFQHLAFG FGVRQCLGRR LAEVEMLLLL HHMLKTFQVE TLRQEDMQMV 490 FRFLLMPSSS PFLTFRPVS

References

[1]	"Molecular cloning and sequence analysis of cDNA encoding rat adrenal cytochrome P-450(11)beta." Nonaka Y., Matsukawa N., Morohashi K., Omura T., Ogihara T., Teraoka H., Okamoto M. FEBS Lett. 255:21-26(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley.
[2]	"Dahl's salt-resistant normotensive rat has mutations in cytochrome P450(11 beta), but the salt-sensitive hypertensive rat does not." Matsukawa N., Nonaka Y., Higaki J., Nagano M., Mikami H., Ogihara T., Okamoto M. J. Biol. Chem. 268:9117-9121(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Dahl salt-resistant. Tissue: Adrenal gland.
[3]	"Isolation and characterization of rat CYP11B genes involved in late steps of mineralo- and glucocorticoid syntheses." Mukai K., Imai M., Shimada H., Ishimura Y. J. Biol. Chem. 268:9130-9137(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sprague-Dawley. Tissue: Testis.
[4]	"Three forms of rat CYP11B genes: 11 beta-hydroxylase gene, aldosterone synthase gene, and a novel gene." Nomura M., Morohashi K., Kirita S., Nonaka Y., Okamoto M., Nawata H., Omura T. J. Biochem. 113:144-152(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[5]	"Cytochrome P450(11 beta): structure-function relationship of the enzyme and its involvement in blood pressure regulation." Okamoto M., Nonaka Y., Ohta M., Takemori H., Halder S.K., Zhi-Nong W., Sun T., Hatano O., Takakusa A., Murakami T. J. Steroid Biochem. Mol. Biol. 53:89-94(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: Dahl salt-resistant.
[6]	"Isolation of aldosterone synthase cytochrome P-450 from zona glomerulosa mitochondria of rat adrenal cortex." Ogishima T., Mitani F., Ishimura Y. J. Biol. Chem. 264:10935-10938(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-44. Tissue: Adrenal cortex.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D11354 mRNA. Translation: BAA01957.1. D14091 Genomic DNA. Translation: BAA03171.1. X15431 mRNA. Translation: CAA33472.1. D10107 mRNA. Translation: BAA00988.1.
IPI	IPI00734638.
PIR	A46039.
UniGene	Rn.198235.
3D structure databases
ProteinModelPortal	P15393.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000039857.
Proteomic databases
PaxDb	P15393.
PRIDE	P15393.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
UCSC	RGD:2453. rat.
Organism-specific databases
RGD	2453. Cyp11b1.
Phylogenomic databases
eggNOG	COG2124.
HOGENOM	HOG000013161.
HOVERGEN	HBG051098.
InParanoid	P15393.
OrthoDB	EOG4B2SWV.
Enzyme and pathway databases
SABIO-RK	P15393.
Gene expression databases
ArrayExpress	P15393.
Genevestigator	P15393.
GermOnline	ENSRNOG00000032450. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002399. Cyt_P450_mitochondrial. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00408. MITP450. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P15393.
ChEMBL	CHEMBL4970.
NextBio	21682341.

Entry information

Entry name

C11B1_RAT

Accession

Primary (citable) accession number: P15393
Secondary accession number(s): Q64655

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	April 3, 2013
This is version 115 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program