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P15393

- C11B1_RAT

UniProt

P15393 - C11B1_RAT

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Protein

Cytochrome P450 11B1, mitochondrial

Gene

Cyp11b1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Forms corticosterone from 11-deoxycorticosterone.

Catalytic activityi

A steroid + reduced adrenodoxin + O2 = an 11-beta-hydroxysteroid + oxidized adrenodoxin + H2O.

Cofactori

Heme group.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi446 – 4461Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. heme binding Source: InterPro
  2. iron ion binding Source: InterPro
  3. steroid 11-beta-monooxygenase activity Source: RGD
  4. steroid binding Source: RGD

GO - Biological processi

  1. adenosine metabolic process Source: RGD
  2. aldosterone biosynthetic process Source: UniProtKB
  3. cellular response to interferon-gamma Source: RGD
  4. cortisol biosynthetic process Source: UniProtKB
  5. dibenzo-p-dioxin metabolic process Source: RGD
  6. glucocorticoid biosynthetic process Source: RGD
  7. glucocorticoid metabolic process Source: RGD
  8. response to dexamethasone Source: RGD
  9. response to drug Source: RGD
  10. steroid biosynthetic process Source: RGD
  11. steroid metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Steroidogenesis

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

SABIO-RKP15393.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 11B1, mitochondrial
Alternative name(s):
CYPXIB1
Cytochrome P450(11 beta)-DS
Cytochrome P450C11
Steroid 11-beta-hydroxylase (EC:1.14.15.4)
Gene namesi
Name:Cyp11b1
Synonyms:Cyp11b-1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi2453. Cyp11b1.

Subcellular locationi

GO - Cellular componenti

  1. integral component of endoplasmic reticulum membrane Source: RGD
  2. mitochondrion Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2424Mitochondrion1 PublicationAdd
BLAST
Chaini25 – 499475Cytochrome P450 11B1, mitochondrialPRO_0000003603Add
BLAST

Proteomic databases

PaxDbiP15393.
PRIDEiP15393.

Expressioni

Tissue specificityi

Adrenal zona fasciculata/reticularis.

Gene expression databases

GenevestigatoriP15393.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000039857.

Structurei

3D structure databases

ProteinModelPortaliP15393.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000013161.
HOVERGENiHBG051098.
InParanoidiP15393.
PhylomeDBiP15393.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002399. Cyt_P450_mitochondrial.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00408. MITP450.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15393-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALRVTADVW LARPWQCLHR TRALGTTAKV APKTLKPFEA IPQYSRNKWL
60 70 80 90 100
KMIQILREQG QENLHLEMHQ AFQELGPIFR HSAGGAQIVS VMLPEDAEKL
110 120 130 140 150
HQVESILPHR MPLEPWVAHR ELRGLRRGVF LLNGADWRFN RLQLNPNMLS
160 170 180 190 200
PKAIQSFVPF VDVVARDFVE NLKKRMLENV HGSMSINIQS NMFNYTMEAS
210 220 230 240 250
HFVISGERLG LTGHDLKPES VTFTHALHSM FKSTTQLMFL PKSLTRWTST
260 270 280 290 300
RVWKEHFDSW DIISEYVTKC IKNVYRELAE GRQQSWSVIS EMVAQSTLSM
310 320 330 340 350
DAIHANSMEL IAGSVDTTAI SLVMTLFELA RNPDVQQALR QESLAAEASI
360 370 380 390 400
VANPQKAMSD LPLLRAALKE TLRLYPVGSF VERIVHSDLV LQNYHVPAGT
410 420 430 440 450
FVIIYLYSMG RNPAVFPRPE RYMPQRWLER KRSFQHLAFG FGVRQCLGRR
460 470 480 490
LAEVEMLLLL HHMLKTFQVE TLRQEDMQMV FRFLLMPSSS PFLTFRPVS
Length:499
Mass (Da):57,459
Last modified:April 1, 1990 - v1
Checksum:i19C041F96A673C7E
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti127 – 1271R → C in strain: Dahl salt-resistant.
Natural varianti351 – 3511V → A in strain: Dahl salt-resistant.
Natural varianti381 – 3811V → L in strain: Dahl salt-resistant.
Natural varianti384 – 3841I → L in strain: Dahl salt-resistant.
Natural varianti443 – 4431V → M in strain: Dahl salt-resistant.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D11354 mRNA. Translation: BAA01957.1.
D14091 Genomic DNA. Translation: BAA03171.1.
X15431 mRNA. Translation: CAA33472.1.
D10107 mRNA. Translation: BAA00988.1.
PIRiA46039.
UniGeneiRn.198235.

Genome annotation databases

UCSCiRGD:2453. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D11354 mRNA. Translation: BAA01957.1 .
D14091 Genomic DNA. Translation: BAA03171.1 .
X15431 mRNA. Translation: CAA33472.1 .
D10107 mRNA. Translation: BAA00988.1 .
PIRi A46039.
UniGenei Rn.198235.

3D structure databases

ProteinModelPortali P15393.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000039857.

Chemistry

BindingDBi P15393.
ChEMBLi CHEMBL4970.

Proteomic databases

PaxDbi P15393.
PRIDEi P15393.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:2453. rat.

Organism-specific databases

RGDi 2453. Cyp11b1.

Phylogenomic databases

eggNOGi COG2124.
HOGENOMi HOG000013161.
HOVERGENi HBG051098.
InParanoidi P15393.
PhylomeDBi P15393.

Enzyme and pathway databases

SABIO-RK P15393.

Miscellaneous databases

NextBioi 21682341.

Gene expression databases

Genevestigatori P15393.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002399. Cyt_P450_mitochondrial.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00408. MITP450.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and sequence analysis of cDNA encoding rat adrenal cytochrome P-450(11)beta."
    Nonaka Y., Matsukawa N., Morohashi K., Omura T., Ogihara T., Teraoka H., Okamoto M.
    FEBS Lett. 255:21-26(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
  2. "Dahl's salt-resistant normotensive rat has mutations in cytochrome P450(11 beta), but the salt-sensitive hypertensive rat does not."
    Matsukawa N., Nonaka Y., Higaki J., Nagano M., Mikami H., Ogihara T., Okamoto M.
    J. Biol. Chem. 268:9117-9121(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Dahl salt-resistant.
    Tissue: Adrenal gland.
  3. "Isolation and characterization of rat CYP11B genes involved in late steps of mineralo- and glucocorticoid syntheses."
    Mukai K., Imai M., Shimada H., Ishimura Y.
    J. Biol. Chem. 268:9130-9137(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Sprague-Dawley.
    Tissue: Testis.
  4. "Three forms of rat CYP11B genes: 11 beta-hydroxylase gene, aldosterone synthase gene, and a novel gene."
    Nomura M., Morohashi K., Kirita S., Nonaka Y., Okamoto M., Nawata H., Omura T.
    J. Biochem. 113:144-152(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  5. "Cytochrome P450(11 beta): structure-function relationship of the enzyme and its involvement in blood pressure regulation."
    Okamoto M., Nonaka Y., Ohta M., Takemori H., Halder S.K., Zhi-Nong W., Sun T., Hatano O., Takakusa A., Murakami T.
    J. Steroid Biochem. Mol. Biol. 53:89-94(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Dahl salt-resistant.
  6. "Isolation of aldosterone synthase cytochrome P-450 from zona glomerulosa mitochondria of rat adrenal cortex."
    Ogishima T., Mitani F., Ishimura Y.
    J. Biol. Chem. 264:10935-10938(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-44.
    Tissue: Adrenal cortex.

Entry informationi

Entry nameiC11B1_RAT
AccessioniPrimary (citable) accession number: P15393
Secondary accession number(s): Q64655
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: October 29, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3