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P15393 (C11B1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 11B1, mitochondrial
Alternative name(s):
CYPXIB1
Cytochrome P450(11 beta)-DS
Cytochrome P450C11
Steroid 11-beta-hydroxylase
EC=1.14.15.4
Gene names
Name:Cyp11b1
Synonyms:Cyp11b-1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length499 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Forms corticosterone from 11-deoxycorticosterone.

Catalytic activity

A steroid + reduced adrenodoxin + O2 = an 11-beta-hydroxysteroid + oxidized adrenodoxin + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Mitochondrion membrane.

Tissue specificity

Adrenal zona fasciculata/reticularis.

Sequence similarities

Belongs to the cytochrome P450 family.

Ontologies

Keywords
   Biological processSteroidogenesis
   Cellular componentMembrane
Mitochondrion
   DomainTransit peptide
   LigandHeme
Iron
Metal-binding
   Molecular functionMonooxygenase
Oxidoreductase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processadenosine metabolic process

Inferred from expression pattern PubMed 20937274. Source: RGD

aldosterone biosynthetic process

Inferred from mutant phenotype PubMed 19342457. Source: UniProtKB

cellular response to interferon-gamma

Inferred from expression pattern PubMed 12121434. Source: RGD

cortisol biosynthetic process

Inferred from mutant phenotype PubMed 19342457. Source: UniProtKB

dibenzo-p-dioxin metabolic process

Inferred from expression pattern PubMed 16254025. Source: RGD

glucocorticoid biosynthetic process

Inferred from direct assay PubMed 19665544. Source: RGD

glucocorticoid metabolic process

Traceable author statement PubMed 11796518. Source: RGD

response to dexamethasone

Inferred from expression pattern PubMed 18252953. Source: RGD

response to drug

Inferred from expression pattern PubMed 19733641. Source: RGD

steroid biosynthetic process

Inferred from direct assay PubMed 16405651. Source: RGD

steroid metabolic process

Traceable author statement PubMed 16179417. Source: RGD

   Cellular_componentintegral component of endoplasmic reticulum membrane

Inferred from direct assay PubMed 11796518. Source: RGD

mitochondrial membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

mitochondrion

Inferred from direct assay PubMed 8674848. Source: RGD

   Molecular_functionheme binding

Inferred from electronic annotation. Source: InterPro

iron ion binding

Inferred from electronic annotation. Source: InterPro

steroid 11-beta-monooxygenase activity

Inferred from direct assay PubMed 11796518PubMed 16405651. Source: RGD

steroid binding

Inferred from direct assay PubMed 16405651. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2424Mitochondrion Ref.6
Chain25 – 499475Cytochrome P450 11B1, mitochondrial
PRO_0000003603

Sites

Metal binding4461Iron (heme axial ligand) By similarity

Natural variations

Natural variant1271R → C in strain: Dahl salt-resistant.
Natural variant3511V → A in strain: Dahl salt-resistant.
Natural variant3811V → L in strain: Dahl salt-resistant.
Natural variant3841I → L in strain: Dahl salt-resistant.
Natural variant4431V → M in strain: Dahl salt-resistant.

Sequences

Sequence LengthMass (Da)Tools
P15393 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: 19C041F96A673C7E

FASTA49957,459
        10         20         30         40         50         60 
MALRVTADVW LARPWQCLHR TRALGTTAKV APKTLKPFEA IPQYSRNKWL KMIQILREQG 

        70         80         90        100        110        120 
QENLHLEMHQ AFQELGPIFR HSAGGAQIVS VMLPEDAEKL HQVESILPHR MPLEPWVAHR 

       130        140        150        160        170        180 
ELRGLRRGVF LLNGADWRFN RLQLNPNMLS PKAIQSFVPF VDVVARDFVE NLKKRMLENV 

       190        200        210        220        230        240 
HGSMSINIQS NMFNYTMEAS HFVISGERLG LTGHDLKPES VTFTHALHSM FKSTTQLMFL 

       250        260        270        280        290        300 
PKSLTRWTST RVWKEHFDSW DIISEYVTKC IKNVYRELAE GRQQSWSVIS EMVAQSTLSM 

       310        320        330        340        350        360 
DAIHANSMEL IAGSVDTTAI SLVMTLFELA RNPDVQQALR QESLAAEASI VANPQKAMSD 

       370        380        390        400        410        420 
LPLLRAALKE TLRLYPVGSF VERIVHSDLV LQNYHVPAGT FVIIYLYSMG RNPAVFPRPE 

       430        440        450        460        470        480 
RYMPQRWLER KRSFQHLAFG FGVRQCLGRR LAEVEMLLLL HHMLKTFQVE TLRQEDMQMV 

       490 
FRFLLMPSSS PFLTFRPVS 

« Hide

References

[1]"Molecular cloning and sequence analysis of cDNA encoding rat adrenal cytochrome P-450(11)beta."
Nonaka Y., Matsukawa N., Morohashi K., Omura T., Ogihara T., Teraoka H., Okamoto M.
FEBS Lett. 255:21-26(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
[2]"Dahl's salt-resistant normotensive rat has mutations in cytochrome P450(11 beta), but the salt-sensitive hypertensive rat does not."
Matsukawa N., Nonaka Y., Higaki J., Nagano M., Mikami H., Ogihara T., Okamoto M.
J. Biol. Chem. 268:9117-9121(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Dahl salt-resistant.
Tissue: Adrenal gland.
[3]"Isolation and characterization of rat CYP11B genes involved in late steps of mineralo- and glucocorticoid syntheses."
Mukai K., Imai M., Shimada H., Ishimura Y.
J. Biol. Chem. 268:9130-9137(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Sprague-Dawley.
Tissue: Testis.
[4]"Three forms of rat CYP11B genes: 11 beta-hydroxylase gene, aldosterone synthase gene, and a novel gene."
Nomura M., Morohashi K., Kirita S., Nonaka Y., Okamoto M., Nawata H., Omura T.
J. Biochem. 113:144-152(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[5]"Cytochrome P450(11 beta): structure-function relationship of the enzyme and its involvement in blood pressure regulation."
Okamoto M., Nonaka Y., Ohta M., Takemori H., Halder S.K., Zhi-Nong W., Sun T., Hatano O., Takakusa A., Murakami T.
J. Steroid Biochem. Mol. Biol. 53:89-94(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: Dahl salt-resistant.
[6]"Isolation of aldosterone synthase cytochrome P-450 from zona glomerulosa mitochondria of rat adrenal cortex."
Ogishima T., Mitani F., Ishimura Y.
J. Biol. Chem. 264:10935-10938(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 25-44.
Tissue: Adrenal cortex.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D11354 mRNA. Translation: BAA01957.1.
D14091 Genomic DNA. Translation: BAA03171.1.
X15431 mRNA. Translation: CAA33472.1.
D10107 mRNA. Translation: BAA00988.1.
PIRA46039.
UniGeneRn.198235.

3D structure databases

ProteinModelPortalP15393.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000039857.

Chemistry

BindingDBP15393.
ChEMBLCHEMBL4970.

Proteomic databases

PaxDbP15393.
PRIDEP15393.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:2453. rat.

Organism-specific databases

RGD2453. Cyp11b1.

Phylogenomic databases

eggNOGCOG2124.
HOGENOMHOG000013161.
HOVERGENHBG051098.
InParanoidP15393.
PhylomeDBP15393.

Enzyme and pathway databases

SABIO-RKP15393.

Gene expression databases

GenevestigatorP15393.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002399. Cyt_P450_mitochondrial.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00408. MITP450.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio21682341.

Entry information

Entry nameC11B1_RAT
AccessionPrimary (citable) accession number: P15393
Secondary accession number(s): Q64655
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: April 16, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families