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P15393

- C11B1_RAT

UniProt

P15393 - C11B1_RAT

Protein

Cytochrome P450 11B1, mitochondrial

Gene

Cyp11b1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 1 (01 Apr 1990)
      Previous versions | rss
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    Functioni

    Forms corticosterone from 11-deoxycorticosterone.

    Catalytic activityi

    A steroid + reduced adrenodoxin + O2 = an 11-beta-hydroxysteroid + oxidized adrenodoxin + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi446 – 4461Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. heme binding Source: InterPro
    2. iron ion binding Source: InterPro
    3. steroid 11-beta-monooxygenase activity Source: RGD
    4. steroid binding Source: RGD

    GO - Biological processi

    1. adenosine metabolic process Source: RGD
    2. aldosterone biosynthetic process Source: UniProtKB
    3. cellular response to interferon-gamma Source: RGD
    4. cortisol biosynthetic process Source: UniProtKB
    5. dibenzo-p-dioxin metabolic process Source: RGD
    6. glucocorticoid biosynthetic process Source: RGD
    7. glucocorticoid metabolic process Source: RGD
    8. response to dexamethasone Source: RGD
    9. response to drug Source: RGD
    10. steroid biosynthetic process Source: RGD
    11. steroid metabolic process Source: RGD

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Steroidogenesis

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    SABIO-RKP15393.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 11B1, mitochondrial
    Alternative name(s):
    CYPXIB1
    Cytochrome P450(11 beta)-DS
    Cytochrome P450C11
    Steroid 11-beta-hydroxylase (EC:1.14.15.4)
    Gene namesi
    Name:Cyp11b1
    Synonyms:Cyp11b-1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi2453. Cyp11b1.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of endoplasmic reticulum membrane Source: RGD
    2. mitochondrial membrane Source: UniProtKB-SubCell
    3. mitochondrion Source: RGD

    Keywords - Cellular componenti

    Membrane, Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2424Mitochondrion1 PublicationAdd
    BLAST
    Chaini25 – 499475Cytochrome P450 11B1, mitochondrialPRO_0000003603Add
    BLAST

    Proteomic databases

    PaxDbiP15393.
    PRIDEiP15393.

    Expressioni

    Tissue specificityi

    Adrenal zona fasciculata/reticularis.

    Gene expression databases

    GenevestigatoriP15393.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000039857.

    Structurei

    3D structure databases

    ProteinModelPortaliP15393.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG2124.
    HOGENOMiHOG000013161.
    HOVERGENiHBG051098.
    InParanoidiP15393.
    PhylomeDBiP15393.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002399. Cyt_P450_mitochondrial.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00408. MITP450.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P15393-1 [UniParc]FASTAAdd to Basket

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    MALRVTADVW LARPWQCLHR TRALGTTAKV APKTLKPFEA IPQYSRNKWL    50
    KMIQILREQG QENLHLEMHQ AFQELGPIFR HSAGGAQIVS VMLPEDAEKL 100
    HQVESILPHR MPLEPWVAHR ELRGLRRGVF LLNGADWRFN RLQLNPNMLS 150
    PKAIQSFVPF VDVVARDFVE NLKKRMLENV HGSMSINIQS NMFNYTMEAS 200
    HFVISGERLG LTGHDLKPES VTFTHALHSM FKSTTQLMFL PKSLTRWTST 250
    RVWKEHFDSW DIISEYVTKC IKNVYRELAE GRQQSWSVIS EMVAQSTLSM 300
    DAIHANSMEL IAGSVDTTAI SLVMTLFELA RNPDVQQALR QESLAAEASI 350
    VANPQKAMSD LPLLRAALKE TLRLYPVGSF VERIVHSDLV LQNYHVPAGT 400
    FVIIYLYSMG RNPAVFPRPE RYMPQRWLER KRSFQHLAFG FGVRQCLGRR 450
    LAEVEMLLLL HHMLKTFQVE TLRQEDMQMV FRFLLMPSSS PFLTFRPVS 499
    Length:499
    Mass (Da):57,459
    Last modified:April 1, 1990 - v1
    Checksum:i19C041F96A673C7E
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti127 – 1271R → C in strain: Dahl salt-resistant.
    Natural varianti351 – 3511V → A in strain: Dahl salt-resistant.
    Natural varianti381 – 3811V → L in strain: Dahl salt-resistant.
    Natural varianti384 – 3841I → L in strain: Dahl salt-resistant.
    Natural varianti443 – 4431V → M in strain: Dahl salt-resistant.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D11354 mRNA. Translation: BAA01957.1.
    D14091 Genomic DNA. Translation: BAA03171.1.
    X15431 mRNA. Translation: CAA33472.1.
    D10107 mRNA. Translation: BAA00988.1.
    PIRiA46039.
    UniGeneiRn.198235.

    Genome annotation databases

    UCSCiRGD:2453. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D11354 mRNA. Translation: BAA01957.1 .
    D14091 Genomic DNA. Translation: BAA03171.1 .
    X15431 mRNA. Translation: CAA33472.1 .
    D10107 mRNA. Translation: BAA00988.1 .
    PIRi A46039.
    UniGenei Rn.198235.

    3D structure databases

    ProteinModelPortali P15393.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000039857.

    Chemistry

    BindingDBi P15393.
    ChEMBLi CHEMBL4970.

    Proteomic databases

    PaxDbi P15393.
    PRIDEi P15393.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:2453. rat.

    Organism-specific databases

    RGDi 2453. Cyp11b1.

    Phylogenomic databases

    eggNOGi COG2124.
    HOGENOMi HOG000013161.
    HOVERGENi HBG051098.
    InParanoidi P15393.
    PhylomeDBi P15393.

    Enzyme and pathway databases

    SABIO-RK P15393.

    Miscellaneous databases

    NextBioi 21682341.

    Gene expression databases

    Genevestigatori P15393.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002399. Cyt_P450_mitochondrial.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00408. MITP450.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and sequence analysis of cDNA encoding rat adrenal cytochrome P-450(11)beta."
      Nonaka Y., Matsukawa N., Morohashi K., Omura T., Ogihara T., Teraoka H., Okamoto M.
      FEBS Lett. 255:21-26(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
    2. "Dahl's salt-resistant normotensive rat has mutations in cytochrome P450(11 beta), but the salt-sensitive hypertensive rat does not."
      Matsukawa N., Nonaka Y., Higaki J., Nagano M., Mikami H., Ogihara T., Okamoto M.
      J. Biol. Chem. 268:9117-9121(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Dahl salt-resistant.
      Tissue: Adrenal gland.
    3. "Isolation and characterization of rat CYP11B genes involved in late steps of mineralo- and glucocorticoid syntheses."
      Mukai K., Imai M., Shimada H., Ishimura Y.
      J. Biol. Chem. 268:9130-9137(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Sprague-Dawley.
      Tissue: Testis.
    4. "Three forms of rat CYP11B genes: 11 beta-hydroxylase gene, aldosterone synthase gene, and a novel gene."
      Nomura M., Morohashi K., Kirita S., Nonaka Y., Okamoto M., Nawata H., Omura T.
      J. Biochem. 113:144-152(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    5. "Cytochrome P450(11 beta): structure-function relationship of the enzyme and its involvement in blood pressure regulation."
      Okamoto M., Nonaka Y., Ohta M., Takemori H., Halder S.K., Zhi-Nong W., Sun T., Hatano O., Takakusa A., Murakami T.
      J. Steroid Biochem. Mol. Biol. 53:89-94(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: Dahl salt-resistant.
    6. "Isolation of aldosterone synthase cytochrome P-450 from zona glomerulosa mitochondria of rat adrenal cortex."
      Ogishima T., Mitani F., Ishimura Y.
      J. Biol. Chem. 264:10935-10938(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-44.
      Tissue: Adrenal cortex.

    Entry informationi

    Entry nameiC11B1_RAT
    AccessioniPrimary (citable) accession number: P15393
    Secondary accession number(s): Q64655
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: April 1, 1990
    Last modified: October 1, 2014
    This is version 125 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3