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P15392 (CP2A4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 2A4

EC=1.14.14.1
Alternative name(s):
CYPIIA4
Cytochrome P450-15-alpha
Cytochrome P450-IIA3.1
Testosterone 15-alpha-hydroxylase
Gene names
Name:Cyp2a4
Synonyms:Cyp2a-4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length494 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Highly active in the 15-alpha-hydroxylation of testosterone. Also active in the 15-alpha-hydroxylation of progesterone and androstenedione. Little or no activity on corticosterone, pregnenolone, dehydroepiandrosterone, estradiol or estriol.

Catalytic activity

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Tissue specificity

Kidney and lung. Expressed in liver, with a strong circadian rhythmicity. Circadian expression is regulated by DBP. Ref.6

Miscellaneous

There are only 11 differences between the sequence of testosterone 15-alpha-hydroxylase and that of coumarin 7-hydroxylase. By site-directed mutagenesis it has been shown that modification of position 209 is sufficient to convert the specificity of the two forms of the enzyme.

Sequence similarities

Belongs to the cytochrome P450 family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 494494Cytochrome P450 2A4
PRO_0000051666

Sites

Metal binding4391Iron (heme axial ligand)

Experimental info

Sequence conflict861T → A in AAA37797. Ref.1
Sequence conflict861T → A in AAA40426. Ref.2
Sequence conflict861T → A in AAA40429. Ref.2
Sequence conflict1291R → S in AAA37797. Ref.1
Sequence conflict1291R → S in AAA40426. Ref.2
Sequence conflict1291R → S in AAA40429. Ref.2
Sequence conflict1331T → A in AAA37797. Ref.1
Sequence conflict1331T → A in AAA40426. Ref.2
Sequence conflict1331T → A in AAA40429. Ref.2
Sequence conflict2961L → Q in AAA40426. Ref.2
Sequence conflict2961L → Q in AAA40429. Ref.2
Sequence conflict3061V → G in AAA40426. Ref.2
Sequence conflict3061V → G in AAA40429. Ref.2
Sequence conflict4671E → A in AAA37797. Ref.1
Sequence conflict4671E → A in AAA40426. Ref.2
Sequence conflict4671E → A in AAA40429. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P15392 [UniParc].

Last modified October 3, 2012. Version 3.
Checksum: B75D17F6EAF4A8EC

FASTA49456,782
        10         20         30         40         50         60 
MLTSGLLLVA AVAFLSVLVL MSVWKQRKLS GKLPPGPTPL PFVGNFLQLN TEQMYNSLMK 

        70         80         90        100        110        120 
ISQRYGPVFT IYLGSRRIVV LCGQETVKEA LVDQAEEFSG RGEQATFDWL FKGYGIAFSS 

       130        140        150        160        170        180 
GERAKQLRRF SITTLRDFGV GKRGIEERIQ EEAGFLIDSF RKTNGAFIDP TFYLSRTVSN 

       190        200        210        220        230        240 
VISSIVFGDR FDYEDKEFLS LLRMMLGSLQ FTATSMGQVY EMFSSVMKHL PGPQQQAFKE 

       250        260        270        280        290        300 
LQGLEDFITK KVEHNQRTLD PNSPRDFIDS FLIRMLEEKK NPNTEFYMKN LVLTTLNLFF 

       310        320        330        340        350        360 
AGTETVSTTL RYGFLLLMKY PDIEAKVHEE IDRVIGRNRQ PKYEDRMKMP YTEAVIHEIQ 

       370        380        390        400        410        420 
RFADLIPMGL ARRVTKDTKF RDFLLPKGTE VFPMLGSVLK DPKFFSNPKD FNPKHFLDDK 

       430        440        450        460        470        480 
GQFKKSDAFV PFSIGKRYCF GEGLARMELF LFLTNIMQNF HFKSTQEPQD IDVSPRLVGF 

       490 
VTIPPTYTMS FLSR 

« Hide

References

« Hide 'large scale' references
[1]"The structure and characterization of type I P-450(15) alpha gene as major steroid 15 alpha-hydroxylase and its comparison with type II P-450(15) alpha gene."
Lindberg R., Burkhart B., Ichikawa T., Negishi M.
J. Biol. Chem. 264:6465-6471(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Liver.
[2]"Reciprocal regulation of sex-dependent expression of testosterone 15 alpha-hydroxylase (P-450(15 alpha)) in liver and kidney of male mice by androgen. Evidence for a single gene."
Squires E.J., Negishi M.
J. Biol. Chem. 263:4166-4171(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Kidney.
[5]"Alteration of mouse cytochrome P450coh substrate specificity by mutation of a single amino-acid residue."
Lindberg R., Negishi M.
Nature 339:632-634(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS.
[6]"Circadian expression of the steroid 15 alpha-hydroxylase (Cyp2a4) and coumarin 7-hydroxylase (Cyp2a5) genes in mouse liver is regulated by the PAR leucine zipper transcription factor DBP."
Lavery D.J., Lopez-Molina L., Margueron R., Fleury-Olela F., Conquet F., Schibler U., Bonfils C.
Mol. Cell. Biol. 19:6488-6499(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M26208 expand/collapse EMBL AC list , M25146, M25147, M26202, M26203, M26205, M26206, M26207 Genomic DNA. Translation: AAA37797.1.
J03549 mRNA. Translation: AAA40426.1.
M19319 mRNA. Translation: AAA40429.1.
AC161798 Genomic DNA. No translation available.
BC010761 mRNA. Translation: AAH10761.1.
BC063778 mRNA. Translation: AAH63778.1.
CCDSCCDS21002.1.
PIRA33531.
S03979.
S16068.
RefSeqNP_034127.2. NM_009997.2.
UniGeneMm.154643.
Mm.389848.

3D structure databases

ProteinModelPortalP15392.
SMRP15392. Positions 31-494.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4080270.
STRING10090.ENSMUSP00000096254.

PTM databases

PhosphoSiteP15392.

Proteomic databases

PaxDbP15392.
PRIDEP15392.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000098657; ENSMUSP00000096254; ENSMUSG00000074254.
GeneID13086.
KEGGmmu:13086.
UCSCuc009fui.1. mouse.

Organism-specific databases

CTD13086.
MGIMGI:88596. Cyp2a4.

Phylogenomic databases

eggNOGCOG2124.
GeneTreeENSGT00670000097868.
HOGENOMHOG000036992.
HOVERGENHBG015789.
InParanoidP15392.
KOK07411.
OMAEAVIHEM.
OrthoDBEOG7RBZ85.
TreeFamTF352043.

Gene expression databases

GenevestigatorP15392.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR01684. EP450ICYP2A.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCyp2a4. mouse.
NextBio283050.
PROP15392.
SOURCESearch...

Entry information

Entry nameCP2A4_MOUSE
AccessionPrimary (citable) accession number: P15392
Secondary accession number(s): Q91XG2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: October 3, 2012
Last modified: July 9, 2014
This is version 110 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot