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Protein

Cytochrome P450 2A4

Gene

Cyp2a4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Highly active in the 15-alpha-hydroxylation of testosterone. Also active in the 15-alpha-hydroxylation of progesterone and androstenedione. Little or no activity on corticosterone, pregnenolone, dehydroepiandrosterone, estradiol or estriol.

Catalytic activityi

RH + [reduced NADPH--hemoprotein reductase] + O2 = ROH + [oxidized NADPH--hemoprotein reductase] + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi439Iron (heme axial ligand)1

GO - Molecular functioni

GO - Biological processi

  • epoxygenase P450 pathway Source: GO_Central
  • response to stilbenoid Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2A4 (EC:1.14.14.1)
Alternative name(s):
CYPIIA4
Cytochrome P450-15-alpha
Cytochrome P450-IIA3.1
Testosterone 15-alpha-hydroxylase
Gene namesi
Name:Cyp2a4
Synonyms:Cyp2a-4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:88596. Cyp2a4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000516661 – 494Cytochrome P450 2A4Add BLAST494

Proteomic databases

PaxDbiP15392.
PeptideAtlasiP15392.
PRIDEiP15392.

PTM databases

iPTMnetiP15392.
PhosphoSitePlusiP15392.

Expressioni

Tissue specificityi

Kidney and lung. Expressed in liver, with a strong circadian rhythmicity. Circadian expression is regulated by DBP.1 Publication

Gene expression databases

BgeeiENSMUSG00000074254.
GenevisibleiP15392. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

MINTiMINT-4080270.
STRINGi10090.ENSMUSP00000096254.

Structurei

3D structure databases

ProteinModelPortaliP15392.
SMRiP15392.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0156. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00760000118775.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiP15392.
KOiK07411.
OrthoDBiEOG091G0BT8.
TreeFamiTF352043.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01684. EP450ICYP2A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P15392-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLTSGLLLVA AVAFLSVLVL MSVWKQRKLS GKLPPGPTPL PFVGNFLQLN
60 70 80 90 100
TEQMYNSLMK ISQRYGPVFT IYLGSRRIVV LCGQETVKEA LVDQAEEFSG
110 120 130 140 150
RGEQATFDWL FKGYGIAFSS GERAKQLRRF SITTLRDFGV GKRGIEERIQ
160 170 180 190 200
EEAGFLIDSF RKTNGAFIDP TFYLSRTVSN VISSIVFGDR FDYEDKEFLS
210 220 230 240 250
LLRMMLGSLQ FTATSMGQVY EMFSSVMKHL PGPQQQAFKE LQGLEDFITK
260 270 280 290 300
KVEHNQRTLD PNSPRDFIDS FLIRMLEEKK NPNTEFYMKN LVLTTLNLFF
310 320 330 340 350
AGTETVSTTL RYGFLLLMKY PDIEAKVHEE IDRVIGRNRQ PKYEDRMKMP
360 370 380 390 400
YTEAVIHEIQ RFADLIPMGL ARRVTKDTKF RDFLLPKGTE VFPMLGSVLK
410 420 430 440 450
DPKFFSNPKD FNPKHFLDDK GQFKKSDAFV PFSIGKRYCF GEGLARMELF
460 470 480 490
LFLTNIMQNF HFKSTQEPQD IDVSPRLVGF VTIPPTYTMS FLSR
Length:494
Mass (Da):56,782
Last modified:October 3, 2012 - v3
Checksum:iB75D17F6EAF4A8EC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti86T → A in AAA37797 (PubMed:2703500).Curated1
Sequence conflicti86T → A in AAA40426 (PubMed:3346244).Curated1
Sequence conflicti86T → A in AAA40429 (PubMed:3346244).Curated1
Sequence conflicti129R → S in AAA37797 (PubMed:2703500).Curated1
Sequence conflicti129R → S in AAA40426 (PubMed:3346244).Curated1
Sequence conflicti129R → S in AAA40429 (PubMed:3346244).Curated1
Sequence conflicti133T → A in AAA37797 (PubMed:2703500).Curated1
Sequence conflicti133T → A in AAA40426 (PubMed:3346244).Curated1
Sequence conflicti133T → A in AAA40429 (PubMed:3346244).Curated1
Sequence conflicti296L → Q in AAA40426 (PubMed:3346244).Curated1
Sequence conflicti296L → Q in AAA40429 (PubMed:3346244).Curated1
Sequence conflicti306V → G in AAA40426 (PubMed:3346244).Curated1
Sequence conflicti306V → G in AAA40429 (PubMed:3346244).Curated1
Sequence conflicti467E → A in AAA37797 (PubMed:2703500).Curated1
Sequence conflicti467E → A in AAA40426 (PubMed:3346244).Curated1
Sequence conflicti467E → A in AAA40429 (PubMed:3346244).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26208
, M25146, M25147, M26202, M26203, M26205, M26206, M26207 Genomic DNA. Translation: AAA37797.1.
J03549 mRNA. Translation: AAA40426.1.
M19319 mRNA. Translation: AAA40429.1.
AC161798 Genomic DNA. No translation available.
BC010761 mRNA. Translation: AAH10761.1.
BC063778 mRNA. Translation: AAH63778.1.
CCDSiCCDS21002.1.
PIRiA33531.
S03979.
S16068.
RefSeqiNP_034127.2. NM_009997.2.
UniGeneiMm.154643.
Mm.389848.

Genome annotation databases

EnsembliENSMUST00000098657; ENSMUSP00000096254; ENSMUSG00000074254.
GeneIDi13086.
KEGGimmu:13086.
UCSCiuc009fui.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26208
, M25146, M25147, M26202, M26203, M26205, M26206, M26207 Genomic DNA. Translation: AAA37797.1.
J03549 mRNA. Translation: AAA40426.1.
M19319 mRNA. Translation: AAA40429.1.
AC161798 Genomic DNA. No translation available.
BC010761 mRNA. Translation: AAH10761.1.
BC063778 mRNA. Translation: AAH63778.1.
CCDSiCCDS21002.1.
PIRiA33531.
S03979.
S16068.
RefSeqiNP_034127.2. NM_009997.2.
UniGeneiMm.154643.
Mm.389848.

3D structure databases

ProteinModelPortaliP15392.
SMRiP15392.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4080270.
STRINGi10090.ENSMUSP00000096254.

PTM databases

iPTMnetiP15392.
PhosphoSitePlusiP15392.

Proteomic databases

PaxDbiP15392.
PeptideAtlasiP15392.
PRIDEiP15392.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000098657; ENSMUSP00000096254; ENSMUSG00000074254.
GeneIDi13086.
KEGGimmu:13086.
UCSCiuc009fui.1. mouse.

Organism-specific databases

CTDi13086.
MGIiMGI:88596. Cyp2a4.

Phylogenomic databases

eggNOGiKOG0156. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00760000118775.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiP15392.
KOiK07411.
OrthoDBiEOG091G0BT8.
TreeFamiTF352043.

Miscellaneous databases

PROiP15392.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000074254.
GenevisibleiP15392. MM.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01684. EP450ICYP2A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCP2A4_MOUSE
AccessioniPrimary (citable) accession number: P15392
Secondary accession number(s): Q91XG2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: October 3, 2012
Last modified: November 30, 2016
This is version 128 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

There are only 11 differences between the sequence of testosterone 15-alpha-hydroxylase and that of coumarin 7-hydroxylase. By site-directed mutagenesis it has been shown that modification of position 209 is sufficient to convert the specificity of the two forms of the enzyme.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.