Cytochrome P450 2A4 - Mus musculus (Mouse)

Contribute

Send feedback

Read comments (?) or add your own

P15392 (CP2A4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 101. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cytochrome P450 2A4
EC=1.14.14.1

Alternative name(s):
CYPIIA4
Cytochrome P450-15-alpha
Cytochrome P450-IIA3.1
Testosterone 15-alpha-hydroxylase

Gene names

Name:	Cyp2a4
Synonyms:	Cyp2a-4

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Highly active in the 15-alpha-hydroxylation of testosterone. Also active in the 15-alpha-hydroxylation of progesterone and androstenedione. Little or no activity on corticosterone, pregnenolone, dehydroepiandrosterone, estradiol or estriol.
Catalytic activity	RH + reduced flavoprotein + O₂ = ROH + oxidized flavoprotein + H₂O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Tissue specificity	Kidney and lung. Expressed in liver, with a strong circadian rhythmicity. Circadian expression is regulated by DBP. Ref.6
Miscellaneous	There are only 11 differences between the sequence of testosterone 15-alpha-hydroxylase and that of coumarin 7-hydroxylase. By site-directed mutagenesis it has been shown that modification of position 209 is sufficient to convert the specificity of the two forms of the enzyme.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to stilbenoid Inferred from expression pattern PubMed 17086191. Source: UniProtKB
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 494

494

Cytochrome P450 2A4

PRO_0000051666

Sites

Metal binding

439

Iron (heme axial ligand)

Experimental info

Sequence conflict

T → A in AAA37797. Ref.1

Sequence conflict

T → A in AAA40426. Ref.2

Sequence conflict

T → A in AAA40429. Ref.2

Sequence conflict

129

R → S in AAA37797. Ref.1

Sequence conflict

129

R → S in AAA40426. Ref.2

Sequence conflict

129

R → S in AAA40429. Ref.2

Sequence conflict

133

T → A in AAA37797. Ref.1

Sequence conflict

133

T → A in AAA40426. Ref.2

Sequence conflict

133

T → A in AAA40429. Ref.2

Sequence conflict

296

L → Q in AAA40426. Ref.2

Sequence conflict

296

L → Q in AAA40429. Ref.2

Sequence conflict

306

V → G in AAA40426. Ref.2

Sequence conflict

306

V → G in AAA40429. Ref.2

Sequence conflict

467

E → A in AAA37797. Ref.1

Sequence conflict

467

E → A in AAA40426. Ref.2

Sequence conflict

467

E → A in AAA40429. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P15392 [UniParc].

Last modified October 3, 2012. Version 3.
Checksum: B75D17F6EAF4A8EC
Show »

FASTA

494

56,782

        10         20         30         40         50         60 
MLTSGLLLVA AVAFLSVLVL MSVWKQRKLS GKLPPGPTPL PFVGNFLQLN TEQMYNSLMK 

        70         80         90        100        110        120 
ISQRYGPVFT IYLGSRRIVV LCGQETVKEA LVDQAEEFSG RGEQATFDWL FKGYGIAFSS 

       130        140        150        160        170        180 
GERAKQLRRF SITTLRDFGV GKRGIEERIQ EEAGFLIDSF RKTNGAFIDP TFYLSRTVSN 

       190        200        210        220        230        240 
VISSIVFGDR FDYEDKEFLS LLRMMLGSLQ FTATSMGQVY EMFSSVMKHL PGPQQQAFKE 

       250        260        270        280        290        300 
LQGLEDFITK KVEHNQRTLD PNSPRDFIDS FLIRMLEEKK NPNTEFYMKN LVLTTLNLFF 

       310        320        330        340        350        360 
AGTETVSTTL RYGFLLLMKY PDIEAKVHEE IDRVIGRNRQ PKYEDRMKMP YTEAVIHEIQ 

       370        380        390        400        410        420 
RFADLIPMGL ARRVTKDTKF RDFLLPKGTE VFPMLGSVLK DPKFFSNPKD FNPKHFLDDK 

       430        440        450        460        470        480 
GQFKKSDAFV PFSIGKRYCF GEGLARMELF LFLTNIMQNF HFKSTQEPQD IDVSPRLVGF 

       490 
VTIPPTYTMS FLSR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The structure and characterization of type I P-450(15) alpha gene as major steroid 15 alpha-hydroxylase and its comparison with type II P-450(15) alpha gene." Lindberg R., Burkhart B., Ichikawa T., Negishi M. J. Biol. Chem. 264:6465-6471(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Liver.
[2]	"Reciprocal regulation of sex-dependent expression of testosterone 15 alpha-hydroxylase (P-450(15 alpha)) in liver and kidney of male mice by androgen. Evidence for a single gene." Squires E.J., Negishi M. J. Biol. Chem. 263:4166-4171(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Kidney.
[5]	"Alteration of mouse cytochrome P450coh substrate specificity by mutation of a single amino-acid residue." Lindberg R., Negishi M. Nature 339:632-634(1989) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS.
[6]	"Circadian expression of the steroid 15 alpha-hydroxylase (Cyp2a4) and coumarin 7-hydroxylase (Cyp2a5) genes in mouse liver is regulated by the PAR leucine zipper transcription factor DBP." Lavery D.J., Lopez-Molina L., Margueron R., Fleury-Olela F., Conquet F., Schibler U., Bonfils C. Mol. Cell. Biol. 19:6488-6499(1999) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M26208 M26207 Genomic DNA. Translation: AAA37797.1. J03549 mRNA. Translation: AAA40426.1. M19319 mRNA. Translation: AAA40429.1. AC161798 Genomic DNA. No translation available. BC010761 mRNA. Translation: AAH10761.1. BC063778 mRNA. Translation: AAH63778.1.
IPI	IPI00323398. IPI00798613.
PIR	A33531. S03979. S16068.
RefSeq	NP_034127.2. NM_009997.2.
UniGene	Mm.154643. Mm.389848.
3D structure databases
ProteinModelPortal	P15392.
SMR	P15392. Positions 31-494.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000096254.
PTM databases
PhosphoSite	P15392.
Proteomic databases
PaxDb	P15392.
PRIDE	P15392.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000098657; ENSMUSP00000096254; ENSMUSG00000074254.
GeneID	13086.
KEGG	mmu:13086.
Organism-specific databases
CTD	13086.
MGI	MGI:88596. Cyp2a4.
Phylogenomic databases
eggNOG	COG2124.
GeneTree	ENSGT00670000097868.
HOGENOM	HOG000036992.
HOVERGEN	HBG015789.
InParanoid	P15392.
KO	K07411.
OrthoDB	EOG4BP1BN.
Gene expression databases
Genevestigator	P15392.
GermOnline	ENSMUSG00000074254. Mus musculus.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008067. Cyt_P450_E_grp-I_CYP2A-like. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01684. EP450ICYP2A. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	Cyp2a4. mouse.
NextBio	283050.
SOURCE	Search...

Entry information

Entry name

CP2A4_MOUSE

Accession

Primary (citable) accession number: P15392
Secondary accession number(s): Q91XG2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	October 3, 2012
Last modified:	May 1, 2013
This is version 101 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents