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P15392

- CP2A4_MOUSE

UniProt

P15392 - CP2A4_MOUSE

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Protein

Cytochrome P450 2A4

Gene
Cyp2a4, Cyp2a-4
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Highly active in the 15-alpha-hydroxylation of testosterone. Also active in the 15-alpha-hydroxylation of progesterone and androstenedione. Little or no activity on corticosterone, pregnenolone, dehydroepiandrosterone, estradiol or estriol.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

Heme group By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi439 – 4391Iron (heme axial ligand)

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro

GO - Biological processi

  1. response to stilbenoid Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2A4 (EC:1.14.14.1)
Alternative name(s):
CYPIIA4
Cytochrome P450-15-alpha
Cytochrome P450-IIA3.1
Testosterone 15-alpha-hydroxylase
Gene namesi
Name:Cyp2a4
Synonyms:Cyp2a-4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:88596. Cyp2a4.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 494494Cytochrome P450 2A4PRO_0000051666Add
BLAST

Proteomic databases

PaxDbiP15392.
PRIDEiP15392.

PTM databases

PhosphoSiteiP15392.

Expressioni

Tissue specificityi

Kidney and lung. Expressed in liver, with a strong circadian rhythmicity. Circadian expression is regulated by DBP.1 Publication

Gene expression databases

GenevestigatoriP15392.

Interactioni

Protein-protein interaction databases

MINTiMINT-4080270.
STRINGi10090.ENSMUSP00000096254.

Structurei

3D structure databases

ProteinModelPortaliP15392.
SMRiP15392. Positions 31-494.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00670000097868.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiP15392.
KOiK07411.
OMAiEAVIHEM.
OrthoDBiEOG7RBZ85.
TreeFamiTF352043.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01684. EP450ICYP2A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P15392-1 [UniParc]FASTAAdd to Basket

« Hide

MLTSGLLLVA AVAFLSVLVL MSVWKQRKLS GKLPPGPTPL PFVGNFLQLN    50
TEQMYNSLMK ISQRYGPVFT IYLGSRRIVV LCGQETVKEA LVDQAEEFSG 100
RGEQATFDWL FKGYGIAFSS GERAKQLRRF SITTLRDFGV GKRGIEERIQ 150
EEAGFLIDSF RKTNGAFIDP TFYLSRTVSN VISSIVFGDR FDYEDKEFLS 200
LLRMMLGSLQ FTATSMGQVY EMFSSVMKHL PGPQQQAFKE LQGLEDFITK 250
KVEHNQRTLD PNSPRDFIDS FLIRMLEEKK NPNTEFYMKN LVLTTLNLFF 300
AGTETVSTTL RYGFLLLMKY PDIEAKVHEE IDRVIGRNRQ PKYEDRMKMP 350
YTEAVIHEIQ RFADLIPMGL ARRVTKDTKF RDFLLPKGTE VFPMLGSVLK 400
DPKFFSNPKD FNPKHFLDDK GQFKKSDAFV PFSIGKRYCF GEGLARMELF 450
LFLTNIMQNF HFKSTQEPQD IDVSPRLVGF VTIPPTYTMS FLSR 494
Length:494
Mass (Da):56,782
Last modified:October 3, 2012 - v3
Checksum:iB75D17F6EAF4A8EC
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti86 – 861T → A in AAA37797. 1 Publication
Sequence conflicti86 – 861T → A in AAA40426. 1 Publication
Sequence conflicti86 – 861T → A in AAA40429. 1 Publication
Sequence conflicti129 – 1291R → S in AAA37797. 1 Publication
Sequence conflicti129 – 1291R → S in AAA40426. 1 Publication
Sequence conflicti129 – 1291R → S in AAA40429. 1 Publication
Sequence conflicti133 – 1331T → A in AAA37797. 1 Publication
Sequence conflicti133 – 1331T → A in AAA40426. 1 Publication
Sequence conflicti133 – 1331T → A in AAA40429. 1 Publication
Sequence conflicti296 – 2961L → Q in AAA40426. 1 Publication
Sequence conflicti296 – 2961L → Q in AAA40429. 1 Publication
Sequence conflicti306 – 3061V → G in AAA40426. 1 Publication
Sequence conflicti306 – 3061V → G in AAA40429. 1 Publication
Sequence conflicti467 – 4671E → A in AAA37797. 1 Publication
Sequence conflicti467 – 4671E → A in AAA40426. 1 Publication
Sequence conflicti467 – 4671E → A in AAA40429. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M26208
, M25146, M25147, M26202, M26203, M26205, M26206, M26207 Genomic DNA. Translation: AAA37797.1.
J03549 mRNA. Translation: AAA40426.1.
M19319 mRNA. Translation: AAA40429.1.
AC161798 Genomic DNA. No translation available.
BC010761 mRNA. Translation: AAH10761.1.
BC063778 mRNA. Translation: AAH63778.1.
CCDSiCCDS21002.1.
PIRiA33531.
S03979.
S16068.
RefSeqiNP_034127.2. NM_009997.2.
UniGeneiMm.154643.
Mm.389848.

Genome annotation databases

EnsembliENSMUST00000098657; ENSMUSP00000096254; ENSMUSG00000074254.
GeneIDi13086.
KEGGimmu:13086.
UCSCiuc009fui.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M26208
, M25146 , M25147 , M26202 , M26203 , M26205 , M26206 , M26207 Genomic DNA. Translation: AAA37797.1 .
J03549 mRNA. Translation: AAA40426.1 .
M19319 mRNA. Translation: AAA40429.1 .
AC161798 Genomic DNA. No translation available.
BC010761 mRNA. Translation: AAH10761.1 .
BC063778 mRNA. Translation: AAH63778.1 .
CCDSi CCDS21002.1.
PIRi A33531.
S03979.
S16068.
RefSeqi NP_034127.2. NM_009997.2.
UniGenei Mm.154643.
Mm.389848.

3D structure databases

ProteinModelPortali P15392.
SMRi P15392. Positions 31-494.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-4080270.
STRINGi 10090.ENSMUSP00000096254.

PTM databases

PhosphoSitei P15392.

Proteomic databases

PaxDbi P15392.
PRIDEi P15392.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000098657 ; ENSMUSP00000096254 ; ENSMUSG00000074254 .
GeneIDi 13086.
KEGGi mmu:13086.
UCSCi uc009fui.1. mouse.

Organism-specific databases

CTDi 13086.
MGIi MGI:88596. Cyp2a4.

Phylogenomic databases

eggNOGi COG2124.
GeneTreei ENSGT00670000097868.
HOGENOMi HOG000036992.
HOVERGENi HBG015789.
InParanoidi P15392.
KOi K07411.
OMAi EAVIHEM.
OrthoDBi EOG7RBZ85.
TreeFami TF352043.

Miscellaneous databases

ChiTaRSi Cyp2a4. mouse.
NextBioi 283050.
PROi P15392.
SOURCEi Search...

Gene expression databases

Genevestigatori P15392.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR01684. EP450ICYP2A.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The structure and characterization of type I P-450(15) alpha gene as major steroid 15 alpha-hydroxylase and its comparison with type II P-450(15) alpha gene."
    Lindberg R., Burkhart B., Ichikawa T., Negishi M.
    J. Biol. Chem. 264:6465-6471(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Liver.
  2. "Reciprocal regulation of sex-dependent expression of testosterone 15 alpha-hydroxylase (P-450(15 alpha)) in liver and kidney of male mice by androgen. Evidence for a single gene."
    Squires E.J., Negishi M.
    J. Biol. Chem. 263:4166-4171(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Kidney.
  5. "Alteration of mouse cytochrome P450coh substrate specificity by mutation of a single amino-acid residue."
    Lindberg R., Negishi M.
    Nature 339:632-634(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS.
  6. "Circadian expression of the steroid 15 alpha-hydroxylase (Cyp2a4) and coumarin 7-hydroxylase (Cyp2a5) genes in mouse liver is regulated by the PAR leucine zipper transcription factor DBP."
    Lavery D.J., Lopez-Molina L., Margueron R., Fleury-Olela F., Conquet F., Schibler U., Bonfils C.
    Mol. Cell. Biol. 19:6488-6499(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiCP2A4_MOUSE
AccessioniPrimary (citable) accession number: P15392
Secondary accession number(s): Q91XG2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: October 3, 2012
Last modified: July 9, 2014
This is version 110 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

There are only 11 differences between the sequence of testosterone 15-alpha-hydroxylase and that of coumarin 7-hydroxylase. By site-directed mutagenesis it has been shown that modification of position 209 is sufficient to convert the specificity of the two forms of the enzyme.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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