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P15390

- SCN4A_RAT

UniProt

P15390 - SCN4A_RAT

Protein

Sodium channel protein type 4 subunit alpha

Gene

Scn4a

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Apr 1990)
      Previous versions | rss
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    Functioni

    This protein mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na+ ions may pass in accordance with their electrochemical gradient. This sodium channel may be present in both denervated and innervated skeletal muscle.

    GO - Molecular functioni

    1. voltage-gated sodium channel activity Source: RefGenome

    GO - Biological processi

    1. cation transport Source: RGD
    2. choline transport Source: RGD
    3. membrane depolarization during action potential Source: RefGenome
    4. neuronal action potential Source: RefGenome
    5. potassium ion transport Source: RGD
    6. sodium ion transmembrane transport Source: RefGenome
    7. sodium ion transport Source: RGD

    Keywords - Molecular functioni

    Ion channel, Sodium channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Sodium transport, Transport

    Keywords - Ligandi

    Sodium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium channel protein type 4 subunit alpha
    Alternative name(s):
    Mu-1
    SkM1
    Sodium channel protein skeletal muscle subunit alpha
    Sodium channel protein type IV subunit alpha
    Voltage-gated sodium channel subunit alpha Nav1.4
    Gene namesi
    Name:Scn4a
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi3636. Scn4a.

    Subcellular locationi

    GO - Cellular componenti

    1. plasma membrane Source: RefGenome
    2. voltage-gated sodium channel complex Source: InterPro

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 18401840Sodium channel protein type 4 subunit alphaPRO_0000048496Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei56 – 561Phosphoserine; by PKASequence Analysis
    Modified residuei251 – 2511Phosphoserine; by PKASequence Analysis
    Glycosylationi288 – 2881N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi291 – 2911N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi297 – 2971N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi303 – 3031N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi309 – 3091N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi315 – 3151N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi327 – 3271N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi356 – 3561N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi502 – 5021N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi954 – 9541N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1198 – 11981N-linked (GlcNAc...)Sequence Analysis
    Modified residuei1321 – 13211Phosphoserine; by PKASequence Analysis
    Modified residuei1321 – 13211Phosphoserine; by PKCBy similarity
    Modified residuei1504 – 15041Phosphoserine; by PKASequence Analysis

    Post-translational modificationi

    Phosphorylation at Ser-1321 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents.By similarity

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiP15390.
    PRIDEiP15390.

    Expressioni

    Gene expression databases

    GenevestigatoriP15390.

    Interactioni

    Subunit structurei

    Muscle sodium channels contain an alpha subunit and a smaller beta subunit. Interacts with the PDZ domain of the syntrophin SNTA1, SNTB1 and SNTB2 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi247751. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP15390.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei130 – 15223Helical; Name=S1 of repeat IAdd
    BLAST
    Transmembranei156 – 17924Helical; Name=S2 of repeat IAdd
    BLAST
    Transmembranei192 – 21221Helical; Name=S3 of repeat IAdd
    BLAST
    Transmembranei214 – 23320Helical; Voltage-sensor; Name=S4 of repeat IAdd
    BLAST
    Transmembranei252 – 27423Helical; Name=S5 of repeat IAdd
    BLAST
    Transmembranei417 – 44428Helical; Name=S6 of repeat IAdd
    BLAST
    Transmembranei571 – 59323Helical; Name=S1 of repeat IIAdd
    BLAST
    Transmembranei602 – 62625Helical; Name=S2 of repeat IIAdd
    BLAST
    Transmembranei634 – 65421Helical; Name=S3 of repeat IIAdd
    BLAST
    Transmembranei661 – 68020Helical; Voltage-sensor; Name=S4 of repeat IIAdd
    BLAST
    Transmembranei697 – 71721Helical; Name=S5 of repeat IIAdd
    BLAST
    Transmembranei771 – 79626Helical; Name=S6 of repeat IIAdd
    BLAST
    Transmembranei1020 – 104324Helical; Name=S1 of repeat IIIAdd
    BLAST
    Transmembranei1057 – 108226Helical; Name=S2 of repeat IIIAdd
    BLAST
    Transmembranei1088 – 111023Helical; Name=S3 of repeat IIIAdd
    BLAST
    Transmembranei1115 – 113622Helical; Voltage-sensor; Name=S4 of repeat IIIAdd
    BLAST
    Transmembranei1156 – 117722Helical; Name=S5 of repeat IIIAdd
    BLAST
    Transmembranei1262 – 128827Helical; Name=S6 of repeat IIIAdd
    BLAST
    Transmembranei1342 – 136524Helical; Name=S1 of repeat IVAdd
    BLAST
    Transmembranei1377 – 140024Helical; Name=S2 of repeat IVAdd
    BLAST
    Transmembranei1407 – 143024Helical; Name=S3 of repeat IVAdd
    BLAST
    Transmembranei1441 – 146222Helical; Voltage-sensor; Name=S4 of repeat IVAdd
    BLAST
    Transmembranei1478 – 150023Helical; Name=S5 of repeat IVAdd
    BLAST
    Transmembranei1567 – 159125Helical; Name=S6 of repeat IVAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati128 – 444317IAdd
    BLAST
    Repeati565 – 790226IIAdd
    BLAST
    Repeati1017 – 1274258IIIAdd
    BLAST
    Repeati1342 – 1588247IVAdd
    BLAST
    Domaini1720 – 174930IQPROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.

    Sequence similaritiesi

    Contains 1 IQ domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1226.
    HOGENOMiHOG000231755.
    HOVERGENiHBG053100.
    InParanoidiO70611.
    KOiK04837.
    PhylomeDBiP15390.

    Family and domain databases

    Gene3Di1.20.120.350. 4 hits.
    InterProiIPR027359. Channel_four-helix_dom.
    IPR005821. Ion_trans_dom.
    IPR000048. IQ_motif_EF-hand-BS.
    IPR028826. Na_channel_a4su.
    IPR008052. Na_channel_a4su_mammal.
    IPR001696. Na_channel_asu.
    IPR010526. Na_trans_assoc.
    [Graphical view]
    PANTHERiPTHR10037:SF193. PTHR10037:SF193. 1 hit.
    PfamiPF00520. Ion_trans. 4 hits.
    PF06512. Na_trans_assoc. 1 hit.
    [Graphical view]
    PRINTSiPR00170. NACHANNEL.
    PR01665. NACHANNEL4.
    SMARTiSM00015. IQ. 1 hit.
    [Graphical view]
    PROSITEiPS50096. IQ. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P15390-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASSSLPNLV PPGPHCLRPF TPESLAAIEQ RAVEEEARLQ RNKQMEIEEP     50
    ERKPRSDLEA GKNLPLIYGD PPPEVIGIPL EDLDPYYSDK KTFIVLNKGK 100
    AIFRFSATPA LYLLSPFSIV RRVAIKVLIH ALFSMFIMIT ILTNCVFMTM 150
    SNPPSWSKHV EYTFTGIYTF ESLIKMLARG FCIDDFTFLR DPWNWLDFSV 200
    ITMAYVTEFV DLGNISALRT FRVLRALKTI TVIPGLKTIV GALIQSVKKL 250
    SDVMILTVFC LSVFALVGLQ LFMGNLRQKC VRWPPPMNDT NTTWYGNDTW 300
    YSNDTWYGND TWYINDTWNS QESWAGNSTF DWEAYINDEG NFYFLEGSND 350
    ALLCGNSSDA GHCPEGYECI KAGRNPNYGY TSYDTFSWAF LALFRLMTQD 400
    YWENLFQLTL RAAGKTYMIF FVVIIFLGSF YLINLILAVV AMAYAEQNEA 450
    TLAEDQEKEE EFQQMLEKYK KHQEELEKAK AAQALESGEE ADGDPTHNKD 500
    CNGSLDASGE KGPPRPSCSA DSAISDAMEE LEEAHQKCPP WWYKCAHKVL 550
    IWNCCAPWVK FKHIIYLIVM DPFVDLGITI CIVLNTLFMA MEHYPMTEHF 600
    DNVLSVGNLV FTGIFTAEMV LKLIAMDPYE YFQQGWNIFD SFIVTLSLVE 650
    LGLANVQGLS VLRSFRLLRV FKLAKSWPTL NMLIKIIGNS VGALGNLTLV 700
    LAIIVFIFAV VGMQLFGKSY KECVCKIASD CNLPRWHMND FFHSFLIVFR 750
    ILCGEWIETM WDCMEVAGQA MCLTVFLMVM VIGNLVVLNL FLALLLSSFS 800
    ADSLAASDED GEMNNLQIAI GRIKWGIGFA KTFLLGLLRG KILSPKEIIL 850
    SLGEPGGAGE NAEESTPEDE KKEPPPEDKE LKDNHILNHV GLTDGPRSSI 900
    ELDHLNFINN PYLTIQVPIA SEESDLEMPT EEETDAFSEP EDIKKPLQPL 950
    YDGNSSVCST ADYKPPEEDP EEQAEENPEG EQPEECFTEA CVKRCPCLYV 1000
    DISQGRGKMW WTLRRACFKI VEHNWFETFI VFMILLSSGA LAFEDIYIEQ 1050
    RRVIRTILEY ADKVFTYIFI LEMLLKWVAY GFKVYFTNAW CWLDFLIVDV 1100
    SIISLVANWL GYSELGPIKS LRTLRALRPL RALSRFEGMR VVVNALLGAI 1150
    PSIMNVLLVC LIFWLIFSIM GVNLFAGKFY YCVNTTTSER FDISVVNNKS 1200
    ESESLMYTGQ VRWMNVKVNY DNVGLGYLSL LQVATFKGWM DIMYAAVDSR 1250
    EKEEQPHYEV NLYMYLYFVI FIIFGSFFTL NLFIGVIIDN FNQQKKKFGG 1300
    KDIFMTEEQK KYYNAMKKLG SKKPQKPIPR PQNKIQGMVY DFVTKQVFDI 1350
    SIMILICLNM VTMMVETDDQ SQLKVDILYN INMVFIIIFT GECVLKMFAL 1400
    RHYYFTIGWN IFDFVVVILS IVGLALSDLI QKYFVSPTLF RVIRLARIGR 1450
    VLRLIRGAKG IRTLLFALMM SLPALFNIGL LLFLVMFIYS IFGMSNFAYV 1500
    KKESGIDDMF NFETFGNSII CLFEITTSAG WDGLLNPILN SGPPDCDPTL 1550
    ENPGTNVRGD CGNPSIGICF FCSYIIISFL IVVNMYIAII LENFNVATEE 1600
    SSEPLSEDDF EMFYETWEKF DPDATQFIDY SRLSDFVDTL QEPLKIAKPN 1650
    KIKLITLDLP MVPGDKIHCL DILFALTKEV LGDSGEMDAL KQTMEEKFMA 1700
    ANPSKVSYEP ITTTLKRKQE EVCAIKIQRA YRRHLLQRSV KQASYMYRHS 1750
    QDGNDDGAPE KEGLLANTMN KMYGHEKEGD GVQSQGEEEK ASTEDAGPTV 1800
    EPEPTSSSDT ALTPSPPPLP PSSSPPQGQT VRPGVKESLV 1840
    Length:1,840
    Mass (Da):208,867
    Last modified:April 1, 1990 - v1
    Checksum:iC5DC09D93DD9FAD6
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti120 – 1201V → I in strain: COP; prostatic cancer cell lines. 1 Publication
    Natural varianti916 – 9161Q → H in strain: COP; prostatic cancer cell lines. 1 Publication
    Natural varianti1202 – 12021S → C in strain: COP; prostatic cancer cell lines. 1 Publication
    Natural varianti1257 – 12571H → D in strain: COP; prostatic cancer cell lines. 1 Publication
    Natural varianti1755 – 17551D → G in strain: COP; prostatic cancer cell lines. 1 Publication
    Natural varianti1803 – 18031E → K in strain: COP; prostatic cancer cell lines. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M26643 mRNA. Translation: AAA41682.1.
    Y17153 mRNA. Translation: CAA76659.1.
    PIRiJN0007. CHRTM1.
    RefSeqiNP_037310.1. NM_013178.1.
    UniGeneiRn.9700.

    Genome annotation databases

    GeneIDi25722.
    KEGGirno:25722.
    UCSCiRGD:3636. rat.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M26643 mRNA. Translation: AAA41682.1 .
    Y17153 mRNA. Translation: CAA76659.1 .
    PIRi JN0007. CHRTM1.
    RefSeqi NP_037310.1. NM_013178.1.
    UniGenei Rn.9700.

    3D structure databases

    ProteinModelPortali P15390.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 247751. 1 interaction.

    Chemistry

    BindingDBi P15390.
    ChEMBLi CHEMBL3509.
    GuidetoPHARMACOLOGYi 581.

    Proteomic databases

    PaxDbi P15390.
    PRIDEi P15390.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 25722.
    KEGGi rno:25722.
    UCSCi RGD:3636. rat.

    Organism-specific databases

    CTDi 6329.
    RGDi 3636. Scn4a.

    Phylogenomic databases

    eggNOGi COG1226.
    HOGENOMi HOG000231755.
    HOVERGENi HBG053100.
    InParanoidi O70611.
    KOi K04837.
    PhylomeDBi P15390.

    Miscellaneous databases

    NextBioi 607821.
    PROi P15390.

    Gene expression databases

    Genevestigatori P15390.

    Family and domain databases

    Gene3Di 1.20.120.350. 4 hits.
    InterProi IPR027359. Channel_four-helix_dom.
    IPR005821. Ion_trans_dom.
    IPR000048. IQ_motif_EF-hand-BS.
    IPR028826. Na_channel_a4su.
    IPR008052. Na_channel_a4su_mammal.
    IPR001696. Na_channel_asu.
    IPR010526. Na_trans_assoc.
    [Graphical view ]
    PANTHERi PTHR10037:SF193. PTHR10037:SF193. 1 hit.
    Pfami PF00520. Ion_trans. 4 hits.
    PF06512. Na_trans_assoc. 1 hit.
    [Graphical view ]
    PRINTSi PR00170. NACHANNEL.
    PR01665. NACHANNEL4.
    SMARTi SM00015. IQ. 1 hit.
    [Graphical view ]
    PROSITEi PS50096. IQ. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure and functional expression of a mammalian skeletal muscle sodium channel."
      Trimmer J.S., Cooperman S.S., Tomiko S.A., Zhou J., Crean S.M., Boyle M.B., Kallen R.G., Sheng Z., Barchi R.L., Sigworth F.J., Goodman R.H., Agnew W.S., Mandel G.
      Neuron 3:33-49(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Expression of skeletal muscle-type voltage-gated Na+ channel in rat and human prostate cancer cell lines."
      Diss J.K.J., Stewart D., Fraser S.P., Black J.A., Dibb-Hajj S., Waxman S.G., Archer S.N., Djamgoz M.B.A.
      FEBS Lett. 427:5-10(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS ILE-120; HIS-916; CYS-1202; ASP-1257; GLY-1755 AND LYS-1803.
      Strain: COP.
      Tissue: Prostate.

    Entry informationi

    Entry nameiSCN4A_RAT
    AccessioniPrimary (citable) accession number: P15390
    Secondary accession number(s): O70611
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: April 1, 1990
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3