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P15390

- SCN4A_RAT

UniProt

P15390 - SCN4A_RAT

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Protein

Sodium channel protein type 4 subunit alpha

Gene

Scn4a

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

This protein mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na+ ions may pass in accordance with their electrochemical gradient. This sodium channel may be present in both denervated and innervated skeletal muscle.

GO - Molecular functioni

  1. voltage-gated sodium channel activity Source: RefGenome

GO - Biological processi

  1. cation transport Source: RGD
  2. choline transport Source: RGD
  3. membrane depolarization during action potential Source: RefGenome
  4. neuronal action potential Source: RefGenome
  5. potassium ion transport Source: RGD
  6. sodium ion transmembrane transport Source: RefGenome
  7. sodium ion transport Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Sodium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Sodium transport, Transport

Keywords - Ligandi

Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium channel protein type 4 subunit alpha
Alternative name(s):
Mu-1
SkM1
Sodium channel protein skeletal muscle subunit alpha
Sodium channel protein type IV subunit alpha
Voltage-gated sodium channel subunit alpha Nav1.4
Gene namesi
Name:Scn4a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi3636. Scn4a.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei130 – 15223Helical; Name=S1 of repeat IAdd
BLAST
Transmembranei156 – 17924Helical; Name=S2 of repeat IAdd
BLAST
Transmembranei192 – 21221Helical; Name=S3 of repeat IAdd
BLAST
Transmembranei214 – 23320Helical; Voltage-sensor; Name=S4 of repeat IAdd
BLAST
Transmembranei252 – 27423Helical; Name=S5 of repeat IAdd
BLAST
Transmembranei417 – 44428Helical; Name=S6 of repeat IAdd
BLAST
Transmembranei571 – 59323Helical; Name=S1 of repeat IIAdd
BLAST
Transmembranei602 – 62625Helical; Name=S2 of repeat IIAdd
BLAST
Transmembranei634 – 65421Helical; Name=S3 of repeat IIAdd
BLAST
Transmembranei661 – 68020Helical; Voltage-sensor; Name=S4 of repeat IIAdd
BLAST
Transmembranei697 – 71721Helical; Name=S5 of repeat IIAdd
BLAST
Transmembranei771 – 79626Helical; Name=S6 of repeat IIAdd
BLAST
Transmembranei1020 – 104324Helical; Name=S1 of repeat IIIAdd
BLAST
Transmembranei1057 – 108226Helical; Name=S2 of repeat IIIAdd
BLAST
Transmembranei1088 – 111023Helical; Name=S3 of repeat IIIAdd
BLAST
Transmembranei1115 – 113622Helical; Voltage-sensor; Name=S4 of repeat IIIAdd
BLAST
Transmembranei1156 – 117722Helical; Name=S5 of repeat IIIAdd
BLAST
Transmembranei1262 – 128827Helical; Name=S6 of repeat IIIAdd
BLAST
Transmembranei1342 – 136524Helical; Name=S1 of repeat IVAdd
BLAST
Transmembranei1377 – 140024Helical; Name=S2 of repeat IVAdd
BLAST
Transmembranei1407 – 143024Helical; Name=S3 of repeat IVAdd
BLAST
Transmembranei1441 – 146222Helical; Voltage-sensor; Name=S4 of repeat IVAdd
BLAST
Transmembranei1478 – 150023Helical; Name=S5 of repeat IVAdd
BLAST
Transmembranei1567 – 159125Helical; Name=S6 of repeat IVAdd
BLAST

GO - Cellular componenti

  1. plasma membrane Source: RefGenome
  2. voltage-gated sodium channel complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18401840Sodium channel protein type 4 subunit alphaPRO_0000048496Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei56 – 561Phosphoserine; by PKASequence Analysis
Modified residuei251 – 2511Phosphoserine; by PKASequence Analysis
Glycosylationi288 – 2881N-linked (GlcNAc...)Sequence Analysis
Glycosylationi291 – 2911N-linked (GlcNAc...)Sequence Analysis
Glycosylationi297 – 2971N-linked (GlcNAc...)Sequence Analysis
Glycosylationi303 – 3031N-linked (GlcNAc...)Sequence Analysis
Glycosylationi309 – 3091N-linked (GlcNAc...)Sequence Analysis
Glycosylationi315 – 3151N-linked (GlcNAc...)Sequence Analysis
Glycosylationi327 – 3271N-linked (GlcNAc...)Sequence Analysis
Glycosylationi356 – 3561N-linked (GlcNAc...)Sequence Analysis
Glycosylationi502 – 5021N-linked (GlcNAc...)Sequence Analysis
Glycosylationi954 – 9541N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1198 – 11981N-linked (GlcNAc...)Sequence Analysis
Modified residuei1321 – 13211Phosphoserine; by PKASequence Analysis
Modified residuei1321 – 13211Phosphoserine; by PKCBy similarity
Modified residuei1504 – 15041Phosphoserine; by PKASequence Analysis

Post-translational modificationi

Phosphorylation at Ser-1321 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP15390.
PRIDEiP15390.

Expressioni

Gene expression databases

GenevestigatoriP15390.

Interactioni

Subunit structurei

Muscle sodium channels contain an alpha subunit and a smaller beta subunit. Interacts with the PDZ domain of the syntrophin SNTA1, SNTB1 and SNTB2 (By similarity).By similarity

Protein-protein interaction databases

BioGridi247751. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP15390.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati128 – 444317IAdd
BLAST
Repeati565 – 790226IIAdd
BLAST
Repeati1017 – 1274258IIIAdd
BLAST
Repeati1342 – 1588247IVAdd
BLAST
Domaini1720 – 174930IQPROSITE-ProRule annotationAdd
BLAST

Domaini

The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.

Sequence similaritiesi

Contains 1 IQ domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1226.
HOGENOMiHOG000231755.
HOVERGENiHBG053100.
InParanoidiP15390.
KOiK04837.
PhylomeDBiP15390.

Family and domain databases

Gene3Di1.20.120.350. 4 hits.
InterProiIPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR000048. IQ_motif_EF-hand-BS.
IPR028826. Na_channel_a4su.
IPR008052. Na_channel_a4su_mammal.
IPR001696. Na_channel_asu.
IPR010526. Na_trans_assoc.
[Graphical view]
PANTHERiPTHR10037:SF193. PTHR10037:SF193. 1 hit.
PfamiPF00520. Ion_trans. 4 hits.
PF06512. Na_trans_assoc. 1 hit.
[Graphical view]
PRINTSiPR00170. NACHANNEL.
PR01665. NACHANNEL4.
SMARTiSM00015. IQ. 1 hit.
[Graphical view]
PROSITEiPS50096. IQ. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P15390-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASSSLPNLV PPGPHCLRPF TPESLAAIEQ RAVEEEARLQ RNKQMEIEEP
60 70 80 90 100
ERKPRSDLEA GKNLPLIYGD PPPEVIGIPL EDLDPYYSDK KTFIVLNKGK
110 120 130 140 150
AIFRFSATPA LYLLSPFSIV RRVAIKVLIH ALFSMFIMIT ILTNCVFMTM
160 170 180 190 200
SNPPSWSKHV EYTFTGIYTF ESLIKMLARG FCIDDFTFLR DPWNWLDFSV
210 220 230 240 250
ITMAYVTEFV DLGNISALRT FRVLRALKTI TVIPGLKTIV GALIQSVKKL
260 270 280 290 300
SDVMILTVFC LSVFALVGLQ LFMGNLRQKC VRWPPPMNDT NTTWYGNDTW
310 320 330 340 350
YSNDTWYGND TWYINDTWNS QESWAGNSTF DWEAYINDEG NFYFLEGSND
360 370 380 390 400
ALLCGNSSDA GHCPEGYECI KAGRNPNYGY TSYDTFSWAF LALFRLMTQD
410 420 430 440 450
YWENLFQLTL RAAGKTYMIF FVVIIFLGSF YLINLILAVV AMAYAEQNEA
460 470 480 490 500
TLAEDQEKEE EFQQMLEKYK KHQEELEKAK AAQALESGEE ADGDPTHNKD
510 520 530 540 550
CNGSLDASGE KGPPRPSCSA DSAISDAMEE LEEAHQKCPP WWYKCAHKVL
560 570 580 590 600
IWNCCAPWVK FKHIIYLIVM DPFVDLGITI CIVLNTLFMA MEHYPMTEHF
610 620 630 640 650
DNVLSVGNLV FTGIFTAEMV LKLIAMDPYE YFQQGWNIFD SFIVTLSLVE
660 670 680 690 700
LGLANVQGLS VLRSFRLLRV FKLAKSWPTL NMLIKIIGNS VGALGNLTLV
710 720 730 740 750
LAIIVFIFAV VGMQLFGKSY KECVCKIASD CNLPRWHMND FFHSFLIVFR
760 770 780 790 800
ILCGEWIETM WDCMEVAGQA MCLTVFLMVM VIGNLVVLNL FLALLLSSFS
810 820 830 840 850
ADSLAASDED GEMNNLQIAI GRIKWGIGFA KTFLLGLLRG KILSPKEIIL
860 870 880 890 900
SLGEPGGAGE NAEESTPEDE KKEPPPEDKE LKDNHILNHV GLTDGPRSSI
910 920 930 940 950
ELDHLNFINN PYLTIQVPIA SEESDLEMPT EEETDAFSEP EDIKKPLQPL
960 970 980 990 1000
YDGNSSVCST ADYKPPEEDP EEQAEENPEG EQPEECFTEA CVKRCPCLYV
1010 1020 1030 1040 1050
DISQGRGKMW WTLRRACFKI VEHNWFETFI VFMILLSSGA LAFEDIYIEQ
1060 1070 1080 1090 1100
RRVIRTILEY ADKVFTYIFI LEMLLKWVAY GFKVYFTNAW CWLDFLIVDV
1110 1120 1130 1140 1150
SIISLVANWL GYSELGPIKS LRTLRALRPL RALSRFEGMR VVVNALLGAI
1160 1170 1180 1190 1200
PSIMNVLLVC LIFWLIFSIM GVNLFAGKFY YCVNTTTSER FDISVVNNKS
1210 1220 1230 1240 1250
ESESLMYTGQ VRWMNVKVNY DNVGLGYLSL LQVATFKGWM DIMYAAVDSR
1260 1270 1280 1290 1300
EKEEQPHYEV NLYMYLYFVI FIIFGSFFTL NLFIGVIIDN FNQQKKKFGG
1310 1320 1330 1340 1350
KDIFMTEEQK KYYNAMKKLG SKKPQKPIPR PQNKIQGMVY DFVTKQVFDI
1360 1370 1380 1390 1400
SIMILICLNM VTMMVETDDQ SQLKVDILYN INMVFIIIFT GECVLKMFAL
1410 1420 1430 1440 1450
RHYYFTIGWN IFDFVVVILS IVGLALSDLI QKYFVSPTLF RVIRLARIGR
1460 1470 1480 1490 1500
VLRLIRGAKG IRTLLFALMM SLPALFNIGL LLFLVMFIYS IFGMSNFAYV
1510 1520 1530 1540 1550
KKESGIDDMF NFETFGNSII CLFEITTSAG WDGLLNPILN SGPPDCDPTL
1560 1570 1580 1590 1600
ENPGTNVRGD CGNPSIGICF FCSYIIISFL IVVNMYIAII LENFNVATEE
1610 1620 1630 1640 1650
SSEPLSEDDF EMFYETWEKF DPDATQFIDY SRLSDFVDTL QEPLKIAKPN
1660 1670 1680 1690 1700
KIKLITLDLP MVPGDKIHCL DILFALTKEV LGDSGEMDAL KQTMEEKFMA
1710 1720 1730 1740 1750
ANPSKVSYEP ITTTLKRKQE EVCAIKIQRA YRRHLLQRSV KQASYMYRHS
1760 1770 1780 1790 1800
QDGNDDGAPE KEGLLANTMN KMYGHEKEGD GVQSQGEEEK ASTEDAGPTV
1810 1820 1830 1840
EPEPTSSSDT ALTPSPPPLP PSSSPPQGQT VRPGVKESLV
Length:1,840
Mass (Da):208,867
Last modified:April 1, 1990 - v1
Checksum:iC5DC09D93DD9FAD6
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti120 – 1201V → I in strain: COP; prostatic cancer cell lines. 1 Publication
Natural varianti916 – 9161Q → H in strain: COP; prostatic cancer cell lines. 1 Publication
Natural varianti1202 – 12021S → C in strain: COP; prostatic cancer cell lines. 1 Publication
Natural varianti1257 – 12571H → D in strain: COP; prostatic cancer cell lines. 1 Publication
Natural varianti1755 – 17551D → G in strain: COP; prostatic cancer cell lines. 1 Publication
Natural varianti1803 – 18031E → K in strain: COP; prostatic cancer cell lines. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26643 mRNA. Translation: AAA41682.1.
Y17153 mRNA. Translation: CAA76659.1.
PIRiJN0007. CHRTM1.
RefSeqiNP_037310.1. NM_013178.1.
UniGeneiRn.9700.

Genome annotation databases

GeneIDi25722.
KEGGirno:25722.
UCSCiRGD:3636. rat.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26643 mRNA. Translation: AAA41682.1 .
Y17153 mRNA. Translation: CAA76659.1 .
PIRi JN0007. CHRTM1.
RefSeqi NP_037310.1. NM_013178.1.
UniGenei Rn.9700.

3D structure databases

ProteinModelPortali P15390.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 247751. 1 interaction.

Chemistry

BindingDBi P15390.
ChEMBLi CHEMBL3509.
GuidetoPHARMACOLOGYi 581.

Proteomic databases

PaxDbi P15390.
PRIDEi P15390.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 25722.
KEGGi rno:25722.
UCSCi RGD:3636. rat.

Organism-specific databases

CTDi 6329.
RGDi 3636. Scn4a.

Phylogenomic databases

eggNOGi COG1226.
HOGENOMi HOG000231755.
HOVERGENi HBG053100.
InParanoidi P15390.
KOi K04837.
PhylomeDBi P15390.

Miscellaneous databases

NextBioi 607821.
PROi P15390.

Gene expression databases

Genevestigatori P15390.

Family and domain databases

Gene3Di 1.20.120.350. 4 hits.
InterProi IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR000048. IQ_motif_EF-hand-BS.
IPR028826. Na_channel_a4su.
IPR008052. Na_channel_a4su_mammal.
IPR001696. Na_channel_asu.
IPR010526. Na_trans_assoc.
[Graphical view ]
PANTHERi PTHR10037:SF193. PTHR10037:SF193. 1 hit.
Pfami PF00520. Ion_trans. 4 hits.
PF06512. Na_trans_assoc. 1 hit.
[Graphical view ]
PRINTSi PR00170. NACHANNEL.
PR01665. NACHANNEL4.
SMARTi SM00015. IQ. 1 hit.
[Graphical view ]
PROSITEi PS50096. IQ. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Primary structure and functional expression of a mammalian skeletal muscle sodium channel."
    Trimmer J.S., Cooperman S.S., Tomiko S.A., Zhou J., Crean S.M., Boyle M.B., Kallen R.G., Sheng Z., Barchi R.L., Sigworth F.J., Goodman R.H., Agnew W.S., Mandel G.
    Neuron 3:33-49(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Expression of skeletal muscle-type voltage-gated Na+ channel in rat and human prostate cancer cell lines."
    Diss J.K.J., Stewart D., Fraser S.P., Black J.A., Dibb-Hajj S., Waxman S.G., Archer S.N., Djamgoz M.B.A.
    FEBS Lett. 427:5-10(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS ILE-120; HIS-916; CYS-1202; ASP-1257; GLY-1755 AND LYS-1803.
    Strain: COP.
    Tissue: Prostate.

Entry informationi

Entry nameiSCN4A_RAT
AccessioniPrimary (citable) accession number: P15390
Secondary accession number(s): O70611
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: October 29, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3