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P15390

- SCN4A_RAT

UniProt

P15390 - SCN4A_RAT

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Protein
Sodium channel protein type 4 subunit alpha
Gene
Scn4a
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

This protein mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na+ ions may pass in accordance with their electrochemical gradient. This sodium channel may be present in both denervated and innervated skeletal muscle.

GO - Molecular functioni

  1. voltage-gated sodium channel activity Source: RefGenome

GO - Biological processi

  1. cation transport Source: RGD
  2. choline transport Source: RGD
  3. membrane depolarization during action potential Source: RefGenome
  4. neuronal action potential Source: RefGenome
  5. potassium ion transport Source: RGD
  6. sodium ion transmembrane transport Source: RefGenome
  7. sodium ion transport Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Sodium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Sodium transport, Transport

Keywords - Ligandi

Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium channel protein type 4 subunit alpha
Alternative name(s):
Mu-1
SkM1
Sodium channel protein skeletal muscle subunit alpha
Sodium channel protein type IV subunit alpha
Voltage-gated sodium channel subunit alpha Nav1.4
Gene namesi
Name:Scn4a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi3636. Scn4a.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei130 – 15223Helical; Name=S1 of repeat I
Add
BLAST
Transmembranei156 – 17924Helical; Name=S2 of repeat I
Add
BLAST
Transmembranei192 – 21221Helical; Name=S3 of repeat I
Add
BLAST
Transmembranei214 – 23320Helical; Voltage-sensor; Name=S4 of repeat I
Add
BLAST
Transmembranei252 – 27423Helical; Name=S5 of repeat I
Add
BLAST
Transmembranei417 – 44428Helical; Name=S6 of repeat I
Add
BLAST
Transmembranei571 – 59323Helical; Name=S1 of repeat II
Add
BLAST
Transmembranei602 – 62625Helical; Name=S2 of repeat II
Add
BLAST
Transmembranei634 – 65421Helical; Name=S3 of repeat II
Add
BLAST
Transmembranei661 – 68020Helical; Voltage-sensor; Name=S4 of repeat II
Add
BLAST
Transmembranei697 – 71721Helical; Name=S5 of repeat II
Add
BLAST
Transmembranei771 – 79626Helical; Name=S6 of repeat II
Add
BLAST
Transmembranei1020 – 104324Helical; Name=S1 of repeat III
Add
BLAST
Transmembranei1057 – 108226Helical; Name=S2 of repeat III
Add
BLAST
Transmembranei1088 – 111023Helical; Name=S3 of repeat III
Add
BLAST
Transmembranei1115 – 113622Helical; Voltage-sensor; Name=S4 of repeat III
Add
BLAST
Transmembranei1156 – 117722Helical; Name=S5 of repeat III
Add
BLAST
Transmembranei1262 – 128827Helical; Name=S6 of repeat III
Add
BLAST
Transmembranei1342 – 136524Helical; Name=S1 of repeat IV
Add
BLAST
Transmembranei1377 – 140024Helical; Name=S2 of repeat IV
Add
BLAST
Transmembranei1407 – 143024Helical; Name=S3 of repeat IV
Add
BLAST
Transmembranei1441 – 146222Helical; Voltage-sensor; Name=S4 of repeat IV
Add
BLAST
Transmembranei1478 – 150023Helical; Name=S5 of repeat IV
Add
BLAST
Transmembranei1567 – 159125Helical; Name=S6 of repeat IV
Add
BLAST

GO - Cellular componenti

  1. plasma membrane Source: RefGenome
  2. voltage-gated sodium channel complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18401840Sodium channel protein type 4 subunit alpha
PRO_0000048496Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei56 – 561Phosphoserine; by PKA Reviewed prediction
Modified residuei251 – 2511Phosphoserine; by PKA Reviewed prediction
Glycosylationi288 – 2881N-linked (GlcNAc...) Reviewed prediction
Glycosylationi291 – 2911N-linked (GlcNAc...) Reviewed prediction
Glycosylationi297 – 2971N-linked (GlcNAc...) Reviewed prediction
Glycosylationi303 – 3031N-linked (GlcNAc...) Reviewed prediction
Glycosylationi309 – 3091N-linked (GlcNAc...) Reviewed prediction
Glycosylationi315 – 3151N-linked (GlcNAc...) Reviewed prediction
Glycosylationi327 – 3271N-linked (GlcNAc...) Reviewed prediction
Glycosylationi356 – 3561N-linked (GlcNAc...) Reviewed prediction
Glycosylationi502 – 5021N-linked (GlcNAc...) Reviewed prediction
Glycosylationi954 – 9541N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1198 – 11981N-linked (GlcNAc...) Reviewed prediction
Modified residuei1321 – 13211Phosphoserine; by PKA Reviewed prediction
Modified residuei1321 – 13211Phosphoserine; by PKC By similarity
Modified residuei1504 – 15041Phosphoserine; by PKA Reviewed prediction

Post-translational modificationi

Phosphorylation at Ser-1321 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents By similarity.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP15390.
PRIDEiP15390.

Expressioni

Gene expression databases

GenevestigatoriP15390.

Interactioni

Subunit structurei

Muscle sodium channels contain an alpha subunit and a smaller beta subunit. Interacts with the PDZ domain of the syntrophin SNTA1, SNTB1 and SNTB2 By similarity.

Protein-protein interaction databases

BioGridi247751. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP15390.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati128 – 444317I
Add
BLAST
Repeati565 – 790226II
Add
BLAST
Repeati1017 – 1274258III
Add
BLAST
Repeati1342 – 1588247IV
Add
BLAST
Domaini1720 – 174930IQ
Add
BLAST

Domaini

The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.

Sequence similaritiesi

Contains 1 IQ domain.

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1226.
HOGENOMiHOG000231755.
HOVERGENiHBG053100.
InParanoidiO70611.
KOiK04837.
PhylomeDBiP15390.

Family and domain databases

Gene3Di1.20.120.350. 4 hits.
InterProiIPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR000048. IQ_motif_EF-hand-BS.
IPR028826. Na_channel_a4su.
IPR008052. Na_channel_a4su_mammal.
IPR001696. Na_channel_asu.
IPR010526. Na_trans_assoc.
[Graphical view]
PANTHERiPTHR10037:SF193. PTHR10037:SF193. 1 hit.
PfamiPF00520. Ion_trans. 4 hits.
PF06512. Na_trans_assoc. 1 hit.
[Graphical view]
PRINTSiPR00170. NACHANNEL.
PR01665. NACHANNEL4.
SMARTiSM00015. IQ. 1 hit.
[Graphical view]
PROSITEiPS50096. IQ. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P15390-1 [UniParc]FASTAAdd to Basket

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MASSSLPNLV PPGPHCLRPF TPESLAAIEQ RAVEEEARLQ RNKQMEIEEP     50
ERKPRSDLEA GKNLPLIYGD PPPEVIGIPL EDLDPYYSDK KTFIVLNKGK 100
AIFRFSATPA LYLLSPFSIV RRVAIKVLIH ALFSMFIMIT ILTNCVFMTM 150
SNPPSWSKHV EYTFTGIYTF ESLIKMLARG FCIDDFTFLR DPWNWLDFSV 200
ITMAYVTEFV DLGNISALRT FRVLRALKTI TVIPGLKTIV GALIQSVKKL 250
SDVMILTVFC LSVFALVGLQ LFMGNLRQKC VRWPPPMNDT NTTWYGNDTW 300
YSNDTWYGND TWYINDTWNS QESWAGNSTF DWEAYINDEG NFYFLEGSND 350
ALLCGNSSDA GHCPEGYECI KAGRNPNYGY TSYDTFSWAF LALFRLMTQD 400
YWENLFQLTL RAAGKTYMIF FVVIIFLGSF YLINLILAVV AMAYAEQNEA 450
TLAEDQEKEE EFQQMLEKYK KHQEELEKAK AAQALESGEE ADGDPTHNKD 500
CNGSLDASGE KGPPRPSCSA DSAISDAMEE LEEAHQKCPP WWYKCAHKVL 550
IWNCCAPWVK FKHIIYLIVM DPFVDLGITI CIVLNTLFMA MEHYPMTEHF 600
DNVLSVGNLV FTGIFTAEMV LKLIAMDPYE YFQQGWNIFD SFIVTLSLVE 650
LGLANVQGLS VLRSFRLLRV FKLAKSWPTL NMLIKIIGNS VGALGNLTLV 700
LAIIVFIFAV VGMQLFGKSY KECVCKIASD CNLPRWHMND FFHSFLIVFR 750
ILCGEWIETM WDCMEVAGQA MCLTVFLMVM VIGNLVVLNL FLALLLSSFS 800
ADSLAASDED GEMNNLQIAI GRIKWGIGFA KTFLLGLLRG KILSPKEIIL 850
SLGEPGGAGE NAEESTPEDE KKEPPPEDKE LKDNHILNHV GLTDGPRSSI 900
ELDHLNFINN PYLTIQVPIA SEESDLEMPT EEETDAFSEP EDIKKPLQPL 950
YDGNSSVCST ADYKPPEEDP EEQAEENPEG EQPEECFTEA CVKRCPCLYV 1000
DISQGRGKMW WTLRRACFKI VEHNWFETFI VFMILLSSGA LAFEDIYIEQ 1050
RRVIRTILEY ADKVFTYIFI LEMLLKWVAY GFKVYFTNAW CWLDFLIVDV 1100
SIISLVANWL GYSELGPIKS LRTLRALRPL RALSRFEGMR VVVNALLGAI 1150
PSIMNVLLVC LIFWLIFSIM GVNLFAGKFY YCVNTTTSER FDISVVNNKS 1200
ESESLMYTGQ VRWMNVKVNY DNVGLGYLSL LQVATFKGWM DIMYAAVDSR 1250
EKEEQPHYEV NLYMYLYFVI FIIFGSFFTL NLFIGVIIDN FNQQKKKFGG 1300
KDIFMTEEQK KYYNAMKKLG SKKPQKPIPR PQNKIQGMVY DFVTKQVFDI 1350
SIMILICLNM VTMMVETDDQ SQLKVDILYN INMVFIIIFT GECVLKMFAL 1400
RHYYFTIGWN IFDFVVVILS IVGLALSDLI QKYFVSPTLF RVIRLARIGR 1450
VLRLIRGAKG IRTLLFALMM SLPALFNIGL LLFLVMFIYS IFGMSNFAYV 1500
KKESGIDDMF NFETFGNSII CLFEITTSAG WDGLLNPILN SGPPDCDPTL 1550
ENPGTNVRGD CGNPSIGICF FCSYIIISFL IVVNMYIAII LENFNVATEE 1600
SSEPLSEDDF EMFYETWEKF DPDATQFIDY SRLSDFVDTL QEPLKIAKPN 1650
KIKLITLDLP MVPGDKIHCL DILFALTKEV LGDSGEMDAL KQTMEEKFMA 1700
ANPSKVSYEP ITTTLKRKQE EVCAIKIQRA YRRHLLQRSV KQASYMYRHS 1750
QDGNDDGAPE KEGLLANTMN KMYGHEKEGD GVQSQGEEEK ASTEDAGPTV 1800
EPEPTSSSDT ALTPSPPPLP PSSSPPQGQT VRPGVKESLV 1840
Length:1,840
Mass (Da):208,867
Last modified:April 1, 1990 - v1
Checksum:iC5DC09D93DD9FAD6
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti120 – 1201V → I in strain: COP; prostatic cancer cell lines. 1 Publication
Natural varianti916 – 9161Q → H in strain: COP; prostatic cancer cell lines. 1 Publication
Natural varianti1202 – 12021S → C in strain: COP; prostatic cancer cell lines. 1 Publication
Natural varianti1257 – 12571H → D in strain: COP; prostatic cancer cell lines. 1 Publication
Natural varianti1755 – 17551D → G in strain: COP; prostatic cancer cell lines. 1 Publication
Natural varianti1803 – 18031E → K in strain: COP; prostatic cancer cell lines. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M26643 mRNA. Translation: AAA41682.1.
Y17153 mRNA. Translation: CAA76659.1.
PIRiJN0007. CHRTM1.
RefSeqiNP_037310.1. NM_013178.1.
UniGeneiRn.9700.

Genome annotation databases

GeneIDi25722.
KEGGirno:25722.
UCSCiRGD:3636. rat.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M26643 mRNA. Translation: AAA41682.1 .
Y17153 mRNA. Translation: CAA76659.1 .
PIRi JN0007. CHRTM1.
RefSeqi NP_037310.1. NM_013178.1.
UniGenei Rn.9700.

3D structure databases

ProteinModelPortali P15390.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 247751. 1 interaction.

Chemistry

BindingDBi P15390.
ChEMBLi CHEMBL3509.
GuidetoPHARMACOLOGYi 581.

Proteomic databases

PaxDbi P15390.
PRIDEi P15390.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 25722.
KEGGi rno:25722.
UCSCi RGD:3636. rat.

Organism-specific databases

CTDi 6329.
RGDi 3636. Scn4a.

Phylogenomic databases

eggNOGi COG1226.
HOGENOMi HOG000231755.
HOVERGENi HBG053100.
InParanoidi O70611.
KOi K04837.
PhylomeDBi P15390.

Miscellaneous databases

NextBioi 607821.
PROi P15390.

Gene expression databases

Genevestigatori P15390.

Family and domain databases

Gene3Di 1.20.120.350. 4 hits.
InterProi IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR000048. IQ_motif_EF-hand-BS.
IPR028826. Na_channel_a4su.
IPR008052. Na_channel_a4su_mammal.
IPR001696. Na_channel_asu.
IPR010526. Na_trans_assoc.
[Graphical view ]
PANTHERi PTHR10037:SF193. PTHR10037:SF193. 1 hit.
Pfami PF00520. Ion_trans. 4 hits.
PF06512. Na_trans_assoc. 1 hit.
[Graphical view ]
PRINTSi PR00170. NACHANNEL.
PR01665. NACHANNEL4.
SMARTi SM00015. IQ. 1 hit.
[Graphical view ]
PROSITEi PS50096. IQ. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Primary structure and functional expression of a mammalian skeletal muscle sodium channel."
    Trimmer J.S., Cooperman S.S., Tomiko S.A., Zhou J., Crean S.M., Boyle M.B., Kallen R.G., Sheng Z., Barchi R.L., Sigworth F.J., Goodman R.H., Agnew W.S., Mandel G.
    Neuron 3:33-49(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Expression of skeletal muscle-type voltage-gated Na+ channel in rat and human prostate cancer cell lines."
    Diss J.K.J., Stewart D., Fraser S.P., Black J.A., Dibb-Hajj S., Waxman S.G., Archer S.N., Djamgoz M.B.A.
    FEBS Lett. 427:5-10(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS ILE-120; HIS-916; CYS-1202; ASP-1257; GLY-1755 AND LYS-1803.
    Strain: COP.
    Tissue: Prostate.

Entry informationi

Entry nameiSCN4A_RAT
AccessioniPrimary (citable) accession number: P15390
Secondary accession number(s): O70611
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: June 11, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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