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P15390 (SCN4A_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Sodium channel protein type 4 subunit alpha
Alternative name(s):
Mu-1
SkM1
Sodium channel protein skeletal muscle subunit alpha
Sodium channel protein type IV subunit alpha
Voltage-gated sodium channel subunit alpha Nav1.4
Gene names
Name:Scn4a
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length1840 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

This protein mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na+ ions may pass in accordance with their electrochemical gradient. This sodium channel may be present in both denervated and innervated skeletal muscle.

Subunit structure

Muscle sodium channels contain an alpha subunit and a smaller beta subunit. Interacts with the PDZ domain of the syntrophin SNTA1, SNTB1 and SNTB2 By similarity.

Subcellular location

Membrane; Multi-pass membrane protein.

Domain

The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.

Post-translational modification

Phosphorylation at Ser-1321 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents By similarity.

Sequence similarities

Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.4/SCN4A subfamily. [View classification]

Contains 1 IQ domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 18401840Sodium channel protein type 4 subunit alpha
PRO_0000048496

Regions

Transmembrane130 – 15223Helical; Name=S1 of repeat I
Transmembrane156 – 17924Helical; Name=S2 of repeat I
Transmembrane192 – 21221Helical; Name=S3 of repeat I
Transmembrane214 – 23320Helical; Voltage-sensor; Name=S4 of repeat I
Transmembrane252 – 27423Helical; Name=S5 of repeat I
Transmembrane417 – 44428Helical; Name=S6 of repeat I
Transmembrane571 – 59323Helical; Name=S1 of repeat II
Transmembrane602 – 62625Helical; Name=S2 of repeat II
Transmembrane634 – 65421Helical; Name=S3 of repeat II
Transmembrane661 – 68020Helical; Voltage-sensor; Name=S4 of repeat II
Transmembrane697 – 71721Helical; Name=S5 of repeat II
Transmembrane771 – 79626Helical; Name=S6 of repeat II
Transmembrane1020 – 104324Helical; Name=S1 of repeat III
Transmembrane1057 – 108226Helical; Name=S2 of repeat III
Transmembrane1088 – 111023Helical; Name=S3 of repeat III
Transmembrane1115 – 113622Helical; Voltage-sensor; Name=S4 of repeat III
Transmembrane1156 – 117722Helical; Name=S5 of repeat III
Transmembrane1262 – 128827Helical; Name=S6 of repeat III
Transmembrane1342 – 136524Helical; Name=S1 of repeat IV
Transmembrane1377 – 140024Helical; Name=S2 of repeat IV
Transmembrane1407 – 143024Helical; Name=S3 of repeat IV
Transmembrane1441 – 146222Helical; Voltage-sensor; Name=S4 of repeat IV
Transmembrane1478 – 150023Helical; Name=S5 of repeat IV
Transmembrane1567 – 159125Helical; Name=S6 of repeat IV
Repeat128 – 444317I
Repeat565 – 790226II
Repeat1017 – 1274258III
Repeat1342 – 1588247IV
Domain1720 – 174930IQ

Amino acid modifications

Modified residue561Phosphoserine; by PKA Potential
Modified residue2511Phosphoserine; by PKA Potential
Modified residue13211Phosphoserine; by PKA Potential
Modified residue13211Phosphoserine; by PKC By similarity
Modified residue15041Phosphoserine; by PKA Potential
Glycosylation2881N-linked (GlcNAc...) Potential
Glycosylation2911N-linked (GlcNAc...) Potential
Glycosylation2971N-linked (GlcNAc...) Potential
Glycosylation3031N-linked (GlcNAc...) Potential
Glycosylation3091N-linked (GlcNAc...) Potential
Glycosylation3151N-linked (GlcNAc...) Potential
Glycosylation3271N-linked (GlcNAc...) Potential
Glycosylation3561N-linked (GlcNAc...) Potential
Glycosylation5021N-linked (GlcNAc...) Potential
Glycosylation9541N-linked (GlcNAc...) Potential
Glycosylation11981N-linked (GlcNAc...) Potential

Natural variations

Natural variant1201V → I in strain: COP; prostatic cancer cell lines. Ref.2
Natural variant9161Q → H in strain: COP; prostatic cancer cell lines. Ref.2
Natural variant12021S → C in strain: COP; prostatic cancer cell lines. Ref.2
Natural variant12571H → D in strain: COP; prostatic cancer cell lines. Ref.2
Natural variant17551D → G in strain: COP; prostatic cancer cell lines. Ref.2
Natural variant18031E → K in strain: COP; prostatic cancer cell lines. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P15390 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: C5DC09D93DD9FAD6

FASTA1,840208,867
        10         20         30         40         50         60 
MASSSLPNLV PPGPHCLRPF TPESLAAIEQ RAVEEEARLQ RNKQMEIEEP ERKPRSDLEA 

        70         80         90        100        110        120 
GKNLPLIYGD PPPEVIGIPL EDLDPYYSDK KTFIVLNKGK AIFRFSATPA LYLLSPFSIV 

       130        140        150        160        170        180 
RRVAIKVLIH ALFSMFIMIT ILTNCVFMTM SNPPSWSKHV EYTFTGIYTF ESLIKMLARG 

       190        200        210        220        230        240 
FCIDDFTFLR DPWNWLDFSV ITMAYVTEFV DLGNISALRT FRVLRALKTI TVIPGLKTIV 

       250        260        270        280        290        300 
GALIQSVKKL SDVMILTVFC LSVFALVGLQ LFMGNLRQKC VRWPPPMNDT NTTWYGNDTW 

       310        320        330        340        350        360 
YSNDTWYGND TWYINDTWNS QESWAGNSTF DWEAYINDEG NFYFLEGSND ALLCGNSSDA 

       370        380        390        400        410        420 
GHCPEGYECI KAGRNPNYGY TSYDTFSWAF LALFRLMTQD YWENLFQLTL RAAGKTYMIF 

       430        440        450        460        470        480 
FVVIIFLGSF YLINLILAVV AMAYAEQNEA TLAEDQEKEE EFQQMLEKYK KHQEELEKAK 

       490        500        510        520        530        540 
AAQALESGEE ADGDPTHNKD CNGSLDASGE KGPPRPSCSA DSAISDAMEE LEEAHQKCPP 

       550        560        570        580        590        600 
WWYKCAHKVL IWNCCAPWVK FKHIIYLIVM DPFVDLGITI CIVLNTLFMA MEHYPMTEHF 

       610        620        630        640        650        660 
DNVLSVGNLV FTGIFTAEMV LKLIAMDPYE YFQQGWNIFD SFIVTLSLVE LGLANVQGLS 

       670        680        690        700        710        720 
VLRSFRLLRV FKLAKSWPTL NMLIKIIGNS VGALGNLTLV LAIIVFIFAV VGMQLFGKSY 

       730        740        750        760        770        780 
KECVCKIASD CNLPRWHMND FFHSFLIVFR ILCGEWIETM WDCMEVAGQA MCLTVFLMVM 

       790        800        810        820        830        840 
VIGNLVVLNL FLALLLSSFS ADSLAASDED GEMNNLQIAI GRIKWGIGFA KTFLLGLLRG 

       850        860        870        880        890        900 
KILSPKEIIL SLGEPGGAGE NAEESTPEDE KKEPPPEDKE LKDNHILNHV GLTDGPRSSI 

       910        920        930        940        950        960 
ELDHLNFINN PYLTIQVPIA SEESDLEMPT EEETDAFSEP EDIKKPLQPL YDGNSSVCST 

       970        980        990       1000       1010       1020 
ADYKPPEEDP EEQAEENPEG EQPEECFTEA CVKRCPCLYV DISQGRGKMW WTLRRACFKI 

      1030       1040       1050       1060       1070       1080 
VEHNWFETFI VFMILLSSGA LAFEDIYIEQ RRVIRTILEY ADKVFTYIFI LEMLLKWVAY 

      1090       1100       1110       1120       1130       1140 
GFKVYFTNAW CWLDFLIVDV SIISLVANWL GYSELGPIKS LRTLRALRPL RALSRFEGMR 

      1150       1160       1170       1180       1190       1200 
VVVNALLGAI PSIMNVLLVC LIFWLIFSIM GVNLFAGKFY YCVNTTTSER FDISVVNNKS 

      1210       1220       1230       1240       1250       1260 
ESESLMYTGQ VRWMNVKVNY DNVGLGYLSL LQVATFKGWM DIMYAAVDSR EKEEQPHYEV 

      1270       1280       1290       1300       1310       1320 
NLYMYLYFVI FIIFGSFFTL NLFIGVIIDN FNQQKKKFGG KDIFMTEEQK KYYNAMKKLG 

      1330       1340       1350       1360       1370       1380 
SKKPQKPIPR PQNKIQGMVY DFVTKQVFDI SIMILICLNM VTMMVETDDQ SQLKVDILYN 

      1390       1400       1410       1420       1430       1440 
INMVFIIIFT GECVLKMFAL RHYYFTIGWN IFDFVVVILS IVGLALSDLI QKYFVSPTLF 

      1450       1460       1470       1480       1490       1500 
RVIRLARIGR VLRLIRGAKG IRTLLFALMM SLPALFNIGL LLFLVMFIYS IFGMSNFAYV 

      1510       1520       1530       1540       1550       1560 
KKESGIDDMF NFETFGNSII CLFEITTSAG WDGLLNPILN SGPPDCDPTL ENPGTNVRGD 

      1570       1580       1590       1600       1610       1620 
CGNPSIGICF FCSYIIISFL IVVNMYIAII LENFNVATEE SSEPLSEDDF EMFYETWEKF 

      1630       1640       1650       1660       1670       1680 
DPDATQFIDY SRLSDFVDTL QEPLKIAKPN KIKLITLDLP MVPGDKIHCL DILFALTKEV 

      1690       1700       1710       1720       1730       1740 
LGDSGEMDAL KQTMEEKFMA ANPSKVSYEP ITTTLKRKQE EVCAIKIQRA YRRHLLQRSV 

      1750       1760       1770       1780       1790       1800 
KQASYMYRHS QDGNDDGAPE KEGLLANTMN KMYGHEKEGD GVQSQGEEEK ASTEDAGPTV 

      1810       1820       1830       1840 
EPEPTSSSDT ALTPSPPPLP PSSSPPQGQT VRPGVKESLV 

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References

[1]"Primary structure and functional expression of a mammalian skeletal muscle sodium channel."
Trimmer J.S., Cooperman S.S., Tomiko S.A., Zhou J., Crean S.M., Boyle M.B., Kallen R.G., Sheng Z., Barchi R.L., Sigworth F.J., Goodman R.H., Agnew W.S., Mandel G.
Neuron 3:33-49(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Expression of skeletal muscle-type voltage-gated Na+ channel in rat and human prostate cancer cell lines."
Diss J.K.J., Stewart D., Fraser S.P., Black J.A., Dibb-Hajj S., Waxman S.G., Archer S.N., Djamgoz M.B.A.
FEBS Lett. 427:5-10(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS ILE-120; HIS-916; CYS-1202; ASP-1257; GLY-1755 AND LYS-1803.
Strain: COP.
Tissue: Prostate.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M26643 mRNA. Translation: AAA41682.1.
Y17153 mRNA. Translation: CAA76659.1.
PIRCHRTM1. JN0007.
RefSeqNP_037310.1. NM_013178.1.
UniGeneRn.9700.

3D structure databases

ProteinModelPortalP15390.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid247751. 1 interaction.

Chemistry

BindingDBP15390.
ChEMBLCHEMBL3509.
GuidetoPHARMACOLOGY581.

Proteomic databases

PaxDbP15390.
PRIDEP15390.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID25722.
KEGGrno:25722.
UCSCRGD:3636. rat.

Organism-specific databases

CTD6329.
RGD3636. Scn4a.

Phylogenomic databases

eggNOGCOG1226.
HOGENOMHOG000231755.
HOVERGENHBG053100.
InParanoidO70611.
KOK04837.
PhylomeDBP15390.

Gene expression databases

GenevestigatorP15390.

Family and domain databases

Gene3D1.20.120.350. 4 hits.
InterProIPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR000048. IQ_motif_EF-hand-BS.
IPR008052. Na_channel_a4su_mammal.
IPR028826. Na_channel_a4su_mammal-typ.
IPR001696. Na_channel_asu.
IPR010526. Na_trans_assoc.
[Graphical view]
PANTHERPTHR10037:SF169. PTHR10037:SF169. 1 hit.
PfamPF00520. Ion_trans. 4 hits.
PF06512. Na_trans_assoc. 1 hit.
[Graphical view]
PRINTSPR00170. NACHANNEL.
PR01665. NACHANNEL4.
SMARTSM00015. IQ. 1 hit.
[Graphical view]
PROSITEPS50096. IQ. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio607821.
PROP15390.

Entry information

Entry nameSCN4A_RAT
AccessionPrimary (citable) accession number: P15390
Secondary accession number(s): O70611
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: April 16, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families