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Protein

Potassium voltage-gated channel subfamily C member 1

Gene

Kcnc1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Voltage-gated potassium channel that plays an important role in the rapid repolarization of fast-firing brain neurons. The channel opens in response to the voltage difference across the membrane, forming a potassium-selective channel through which potassium ions pass in accordance with their electrochemical gradient (PubMed:2599109, PubMed:1400413). Can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCNC2, and possibly other family members as well. Contributes to fire sustained trains of very brief action potentials at high frequency in pallidal neurons.By similarity2 Publications

GO - Molecular functioni

  • delayed rectifier potassium channel activity Source: UniProtKB
  • ion channel binding Source: UniProtKB
  • kinesin binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiR-MMU-1296072. Voltage gated Potassium channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium voltage-gated channel subfamily C member 1
Alternative name(s):
NGK21 Publication
Voltage-gated potassium channel subunit Kv3.1
Voltage-gated potassium channel subunit Kv4
Gene namesi
Name:Kcnc1Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:96667. Kcnc1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 190CytoplasmicSequence analysisAdd BLAST190
Transmembranei191 – 209Helical; Name=Segment S1Sequence analysisAdd BLAST19
Transmembranei248 – 267Helical; Name=Segment S2Sequence analysisAdd BLAST20
Topological domaini268 – 276CytoplasmicSequence analysis9
Transmembranei277 – 295Helical; Name=Segment S3Sequence analysisAdd BLAST19
Transmembranei309 – 331Helical; Voltage-sensor; Name=Segment S4Sequence analysisAdd BLAST23
Topological domaini332 – 344CytoplasmicSequence analysisAdd BLAST13
Transmembranei345 – 366Helical; Name=Segment S5Sequence analysisAdd BLAST22
Transmembranei415 – 436Helical; Name=Segment S6Sequence analysisAdd BLAST22
Topological domaini437 – 511CytoplasmicSequence analysisAdd BLAST75

GO - Cellular componenti

  • axolemma Source: MGI
  • dendrite membrane Source: MGI
  • integral component of membrane Source: GO_Central
  • neuronal cell body membrane Source: MGI
  • voltage-gated potassium channel complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutant mice are born at the expected Mendelian rate. They are viable and fertile, but have lower body weight than wild-type. They have normal spontaneous locomotor activity, but impaired motor skills (PubMed:9037088). Mice lacking both Kcnc3 and Kcnc1 are born at the expected Mendelian rate, but the pups do not thrive and all die about 26 days after birth when kept together with other littermates. Their failure to thrive may be due to motor problems; mutant pups survive when fed separately, but 45 days after birth their body weight is only 50 to 60 % of that of wild-type (PubMed:11517255). They appear uncoordinated and display severe ataxia, myoclonus and spontaneous whole-body muscle jerks, but display no obvious alterations in brain morphology (PubMed:11517255, PubMed:15217387, PubMed:16923152). Mutant mice are also much more sensitive to ethanol and fall sideways at ethanol concentrations that have no effect on wild-type mice (PubMed:11517255). They display increased locomotor and exploratory activity (PubMed:11517255, PubMed:15217387). Mice lacking Kcnc1 show reduced response to tremorogenic agent harmaline; mice lacking both Kcnc3 and Kcnc1 are resistant to the tremorogenic agent harmaline (PubMed:15217387).3 Publications

Chemistry databases

GuidetoPHARMACOLOGYi548.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000540521 – 511Potassium voltage-gated channel subfamily C member 1Add BLAST511

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei44PhosphoserineBy similarity1
Modified residuei130PhosphoserineBy similarity1
Modified residuei142PhosphoserineBy similarity1
Modified residuei158PhosphoserineBy similarity1
Modified residuei160PhosphoserineCombined sources1
Glycosylationi220N-linked (GlcNAc...)Sequence analysis1
Glycosylationi229N-linked (GlcNAc...)Sequence analysis1
Modified residuei474PhosphoserineCombined sources1
Modified residuei483PhosphothreonineCombined sources1

Post-translational modificationi

N-glycosylated; contains sialylated glycans.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiE9PVV3.
P15388.
PeptideAtlasiP15388.
PRIDEiP15388.

PTM databases

iPTMnetiP15388.
PhosphoSitePlusiP15388.

Expressioni

Tissue specificityi

Detected in cerebellum (PubMed:11517255, PubMed:15217387). Detected in brain (at protein level) (PubMed:9037088). Detected in brain (PubMed:9037088).3 Publications

Gene expression databases

BgeeiENSMUSG00000058975.
ExpressionAtlasiP15388. baseline and differential.
GenevisibleiP15388. MM.

Interactioni

Subunit structurei

Heteromultimer with KCNG3, KCNG4 and KCNV2 (By similarity). Heteromultimer with KCNC2 (By similarity). Heterotetramer with KCNC3 (By similarity). Interacts with the ancillary subunits KCNE1 and KCNE2; the interaction modulates channel activity (By similarity).By similarity

GO - Molecular functioni

  • ion channel binding Source: UniProtKB
  • kinesin binding Source: MGI

Protein-protein interaction databases

BioGridi200887. 3 interactors.
STRINGi10090.ENSMUSP00000124938.

Structurei

3D structure databases

ProteinModelPortaliP15388.
SMRiP15388.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi400 – 405Selectivity filterBy similarity6

Domaini

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.Curated
The tail may be important in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.Curated

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3713. Eukaryota.
COG1226. LUCA.
GeneTreeiENSGT00760000118846.
HOGENOMiHOG000231012.
HOVERGENiHBG105862.
InParanoidiP15388.
KOiK04887.
OMAiMGQGDDS.
OrthoDBiEOG091G0EJL.
PhylomeDBiP15388.
TreeFamiTF352511.

Family and domain databases

Gene3Di1.20.120.350. 1 hit.
InterProiIPR000210. BTB/POZ_dom.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003968. K_chnl_volt-dep_Kv.
IPR003974. K_chnl_volt-dep_Kv3.
IPR005403. K_chnl_volt-dep_Kv3.1.
IPR011333. SKP1/BTB/POZ.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERiPTHR11537. PTHR11537. 2 hits.
PfamiPF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR00169. KCHANNEL.
PR01581. KV31CHANNEL.
PR01491. KVCHANNEL.
PR01498. SHAWCHANNEL.
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P15388-1) [UniParc]FASTAAdd to basket
Also known as: KV3.1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGQGDESERI VINVGGTRHQ TYRSTLRTLP GTRLAWLAEP DAHSHFDYDP
60 70 80 90 100
RADEFFFDRH PGVFAHILNY YRTGKLHCPA DVCGPLYEEE LAFWGIDETD
110 120 130 140 150
VEPCCWMTYR QHRDAEEALD SFGGAPLDNS ADDADADGPG DSGDGEDELE
160 170 180 190 200
MTKRLALSDS PDGRPGGFWR RWQPRIWALF EDPYSSRYAR YVAFASLFFI
210 220 230 240 250
LVSITTFCLE THERFNPIVN KTEIENVRNG TQVRYYREAE TEAFLTYIEG
260 270 280 290 300
VCVVWFTFEF LMRVVFCPNK VEFIKNSLNI IDFVAILPFY LEVGLSGLSS
310 320 330 340 350
KAAKDVLGFL RVVRFVRILR IFKLTRHFVG LRVLGHTLRA STNEFLLLII
360 370 380 390 400
FLALGVLIFA TMIYYAERIG AQPNDPSASE HTHFKNIPIG FWWAVVTMTT
410 420 430 440 450
LGYGDMYPQT WSGMLVGALC ALAGVLTIAM PVPVIVNNFG MYYSLAMAKQ
460 470 480 490 500
KLPKKKKKHI PRPPQLGSPN YCKSVVNSPH HSTQSDTCPL AQEEILEINR
510
AGRKPLRGMS I
Length:511
Mass (Da):57,928
Last modified:April 1, 1990 - v1
Checksum:i50A939E8F7120F37
GO
Isoform 2 (identifier: P15388-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     502-511: GRKPLRGMSI → DSKLNGEVAK...YMPTEAVRVT

Note: No experimental confirmation available. Gene prediction based on EST data.
Show »
Length:585
Mass (Da):65,857
Checksum:iDD4E2D32848E2DCF
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_058604502 – 511GRKPLRGMSI → DSKLNGEVAKAALANEDCPH IDQALTPDEGLPFTRSGTRE RYGPCFLLSTGEYACPPGGG MRKDLCKESPVIAKYMPTEA VRVT in isoform 2. 10

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y07521 mRNA. Translation: CAA68814.1.
AC020786 Genomic DNA. No translation available.
AC090652 Genomic DNA. No translation available.
CCDSiCCDS21278.1. [P15388-1]
PIRiS07095.
RefSeqiNP_032447.1. NM_008421.3. [P15388-1]
UniGeneiMm.249386.

Genome annotation databases

EnsembliENSMUST00000025202; ENSMUSP00000025202; ENSMUSG00000058975. [P15388-1]
ENSMUST00000160433; ENSMUSP00000124938; ENSMUSG00000058975. [P15388-3]
GeneIDi16502.
KEGGimmu:16502.
UCSCiuc009gyo.2. mouse. [P15388-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y07521 mRNA. Translation: CAA68814.1.
AC020786 Genomic DNA. No translation available.
AC090652 Genomic DNA. No translation available.
CCDSiCCDS21278.1. [P15388-1]
PIRiS07095.
RefSeqiNP_032447.1. NM_008421.3. [P15388-1]
UniGeneiMm.249386.

3D structure databases

ProteinModelPortaliP15388.
SMRiP15388.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200887. 3 interactors.
STRINGi10090.ENSMUSP00000124938.

Chemistry databases

GuidetoPHARMACOLOGYi548.

PTM databases

iPTMnetiP15388.
PhosphoSitePlusiP15388.

Proteomic databases

PaxDbiE9PVV3.
P15388.
PeptideAtlasiP15388.
PRIDEiP15388.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025202; ENSMUSP00000025202; ENSMUSG00000058975. [P15388-1]
ENSMUST00000160433; ENSMUSP00000124938; ENSMUSG00000058975. [P15388-3]
GeneIDi16502.
KEGGimmu:16502.
UCSCiuc009gyo.2. mouse. [P15388-1]

Organism-specific databases

CTDi3746.
MGIiMGI:96667. Kcnc1.

Phylogenomic databases

eggNOGiKOG3713. Eukaryota.
COG1226. LUCA.
GeneTreeiENSGT00760000118846.
HOGENOMiHOG000231012.
HOVERGENiHBG105862.
InParanoidiP15388.
KOiK04887.
OMAiMGQGDDS.
OrthoDBiEOG091G0EJL.
PhylomeDBiP15388.
TreeFamiTF352511.

Enzyme and pathway databases

ReactomeiR-MMU-1296072. Voltage gated Potassium channels.

Miscellaneous databases

PROiP15388.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000058975.
ExpressionAtlasiP15388. baseline and differential.
GenevisibleiP15388. MM.

Family and domain databases

Gene3Di1.20.120.350. 1 hit.
InterProiIPR000210. BTB/POZ_dom.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003968. K_chnl_volt-dep_Kv.
IPR003974. K_chnl_volt-dep_Kv3.
IPR005403. K_chnl_volt-dep_Kv3.1.
IPR011333. SKP1/BTB/POZ.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERiPTHR11537. PTHR11537. 2 hits.
PfamiPF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR00169. KCHANNEL.
PR01581. KV31CHANNEL.
PR01491. KVCHANNEL.
PR01498. SHAWCHANNEL.
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKCNC1_MOUSE
AccessioniPrimary (citable) accession number: P15388
Secondary accession number(s): E9PVV3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: November 30, 2016
This is version 153 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.