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P15384

- KCNA3_RAT

UniProt

P15384 - KCNA3_RAT

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Protein
Potassium voltage-gated channel subfamily A member 3
Gene
Kcna3
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei228 – 2281Not glycosylated

GO - Molecular functioni

  1. delayed rectifier potassium channel activity Source: RGD
  2. outward rectifier potassium channel activity Source: RGD
  3. protein binding Source: IntAct
  4. voltage-gated potassium channel activity Source: RGD

GO - Biological processi

  1. potassium ion transport Source: RGD
  2. protein homooligomerization Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiREACT_199159. Voltage gated Potassium channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium voltage-gated channel subfamily A member 3
Alternative name(s):
RCK3
RGK5
Voltage-gated potassium channel subunit Kv1.3
Voltage-gated potassium channel subunit Kv3
Gene namesi
Name:Kcna3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 2

Organism-specific databases

RGDi2951. Kcna3.

Subcellular locationi

Cell membrane; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 184184Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei185 – 20319Helical; Name=Segment S1; Reviewed prediction
Add
BLAST
Topological domaini204 – 24441Extracellular Reviewed prediction
Add
BLAST
Transmembranei245 – 26622Helical; Name=Segment S2; Reviewed prediction
Add
BLAST
Topological domaini267 – 27711Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei278 – 29821Helical; Name=Segment S3; Reviewed prediction
Add
BLAST
Topological domaini299 – 31214Extracellular Reviewed prediction
Add
BLAST
Transmembranei313 – 33119Helical; Voltage-sensor; Name=Segment S4; Reviewed prediction
Add
BLAST
Topological domaini332 – 34716Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei348 – 36720Helical; Name=Segment S5; Reviewed prediction
Add
BLAST
Topological domaini368 – 40841Extracellular Reviewed prediction
Add
BLAST
Transmembranei409 – 43123Helical; Name=Segment S6; Reviewed prediction
Add
BLAST
Topological domaini432 – 52594Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. membrane raft Source: BHF-UCL
  2. plasma membrane Source: BHF-UCL
  3. voltage-gated potassium channel complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 525525Potassium voltage-gated channel subfamily A member 3
PRO_0000053979Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi229 – 2291N-linked (GlcNAc...)1 Publication
Lipidationi267 – 2671S-palmitoyl cysteine Reviewed prediction
Modified residuei470 – 4701Phosphoserine; by PKA Reviewed prediction

Post-translational modificationi

N-glycosylation promotes the cell surface expression.

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP15384.
PRIDEiP15384.

Expressioni

Gene expression databases

GenevestigatoriP15384.

Interactioni

Subunit structurei

Heterotetramer of potassium channel proteins By similarity. Binds PDZ domains of DLG1, DLG2 and DLG4.

Binary interactionsi

WithEntry#Exp.IntActNotes
DLG4P783522EBI-631478,EBI-80389From a different organism.

Protein-protein interaction databases

IntActiP15384. 1 interaction.
STRINGi10116.ENSRNOP00000024372.

Structurei

3D structure databases

ProteinModelPortaliP15384.
SMRiP15384. Positions 54-441.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi394 – 3996Selectivity filter By similarity
Motifi523 – 5253PDZ-binding

Domaini

The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1226.
GeneTreeiENSGT00740000115256.
HOGENOMiHOG000231015.
HOVERGENiHBG052230.
InParanoidiP15384.
KOiK04876.
OMAiKRRMRYF.
OrthoDBiEOG7M0NRD.
PhylomeDBiP15384.
TreeFamiTF313103.

Family and domain databases

Gene3Di1.20.120.350. 1 hit.
3.30.710.10. 1 hit.
InterProiIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003091. K_chnl.
IPR003968. K_chnl_volt-dep_Kv.
IPR003972. K_chnl_volt-dep_Kv1.
IPR004050. K_chnl_volt-dep_Kv1.3.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERiPTHR11537. PTHR11537. 1 hit.
PfamiPF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR00169. KCHANNEL.
PR01510. KV13CHANNEL.
PR01491. KVCHANNEL.
PR01496. SHAKERCHANEL.
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.

Sequencei

Sequence statusi: Complete.

P15384-1 [UniParc]FASTAAdd to Basket

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MTVVPGDHLL EPEAAGGGGG DPPQGGCVSG GGCDRYEPLP PALPAAGEQD    50
CCGERVVINI SGLRFETQLK TLCQFPETLL GDPKRRMRYF DPLRNEYFFD 100
RNRPSFDAIL YYYQSGGRIR RPVNVPIDIF SEEIRFYQLG EEAMEKFRED 150
EGFLREEERP LPRRDFQRQV WLLFEYPESS GPARGIAIVS VLVILISIVI 200
FCLETLPEFR DEKDYPASPS QDVFEAANNS TSGASSGASS FSDPFFVVET 250
LCIIWFSFEL LVRFFACPSK ATFSRNIMNL IDIVAIIPYF ITLGTELAER 300
QGNGQQAMSL AILRVIRLVR VFRIFKLSRH SKGLQILGQT LKASMRELGL 350
LIFFLFIGVI LFSSAVYFAE ADDPSSGFNS IPDAFWWAVV TMTTVGYGDM 400
HPVTIGGKIV GSLCAIAGVL TIALPVPVIV SNFNYFYHRE TEGEEQAQYM 450
HVGSCQHLSS SAEELRKARS NSTLSKSEYM VIEEGGMNHS AFPQTPFKTG 500
NSTATCTTNN NPNSCVNIKK IFTDV 525
Length:525
Mass (Da):58,425
Last modified:November 1, 1990 - v2
Checksum:i6DA8869D5471C401
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti106 – 1061F → L in CAA34132. 1 Publication
Sequence conflicti181 – 1811G → R in AAA42035. 1 Publication
Sequence conflicti430 – 4301V → L in AAA42035. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X16001 mRNA. Translation: CAA34132.1.
M30312 Genomic DNA. Translation: AAA42035.1.
M31744 Genomic DNA. Translation: AAA41500.1.
PIRiA43531.
RefSeqiNP_062143.3. NM_019270.3.
UniGeneiRn.44292.

Genome annotation databases

EnsembliENSRNOT00000024372; ENSRNOP00000024372; ENSRNOG00000018116.
GeneIDi29731.
KEGGirno:29731.
UCSCiRGD:2951. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X16001 mRNA. Translation: CAA34132.1 .
M30312 Genomic DNA. Translation: AAA42035.1 .
M31744 Genomic DNA. Translation: AAA41500.1 .
PIRi A43531.
RefSeqi NP_062143.3. NM_019270.3.
UniGenei Rn.44292.

3D structure databases

ProteinModelPortali P15384.
SMRi P15384. Positions 54-441.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P15384. 1 interaction.
STRINGi 10116.ENSRNOP00000024372.

Chemistry

ChEMBLi CHEMBL4248.
GuidetoPHARMACOLOGYi 540.

Proteomic databases

PaxDbi P15384.
PRIDEi P15384.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000024372 ; ENSRNOP00000024372 ; ENSRNOG00000018116 .
GeneIDi 29731.
KEGGi rno:29731.
UCSCi RGD:2951. rat.

Organism-specific databases

CTDi 3738.
RGDi 2951. Kcna3.

Phylogenomic databases

eggNOGi COG1226.
GeneTreei ENSGT00740000115256.
HOGENOMi HOG000231015.
HOVERGENi HBG052230.
InParanoidi P15384.
KOi K04876.
OMAi KRRMRYF.
OrthoDBi EOG7M0NRD.
PhylomeDBi P15384.
TreeFami TF313103.

Enzyme and pathway databases

Reactomei REACT_199159. Voltage gated Potassium channels.

Miscellaneous databases

NextBioi 610214.
PROi P15384.

Gene expression databases

Genevestigatori P15384.

Family and domain databases

Gene3Di 1.20.120.350. 1 hit.
3.30.710.10. 1 hit.
InterProi IPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003091. K_chnl.
IPR003968. K_chnl_volt-dep_Kv.
IPR003972. K_chnl_volt-dep_Kv1.
IPR004050. K_chnl_volt-dep_Kv1.3.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view ]
PANTHERi PTHR11537. PTHR11537. 1 hit.
Pfami PF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view ]
PRINTSi PR00169. KCHANNEL.
PR01510. KV13CHANNEL.
PR01491. KVCHANNEL.
PR01496. SHAKERCHANEL.
SMARTi SM00225. BTB. 1 hit.
[Graphical view ]
SUPFAMi SSF54695. SSF54695. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Molecular basis of functional diversity of voltage-gated potassium channels in mammalian brain."
    Stuehmer W., Ruppersberg J.P., Schroerter K.H., Sakmann B., Stocker M., Giese K.P., Perschke A., Baumann A., Pongs O.
    EMBO J. 8:3235-3244(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Cloning and expression of cDNA and genomic clones encoding three delayed rectifier potassium channels in rat brain."
    Swanson R., Marshall J., Smith J., Williams J., Boyle M.B., Folander K., Luneau C.J., Antanavage J., Oliva C., Buhrow S.A., Bennett C., Stein R.B., Kaczmarek L.M.
    Neuron 4:929-939(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Brain.
  3. "Characterization and functional expression of a rat genomic DNA clone encoding a lymphocyte potassium channel."
    Douglass J., Osborne P.B., Cai Y.C., Wilkinson M., Christie M.J., Adelman J.P.
    J. Immunol. 144:4841-4850(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Lymphocyte.
  4. "Clustering of Shaker-type K+ channels by interaction with a family of membrane-associated guanylate kinases."
    Kim E., Niethammer M., Rothschild A., Jan Y.N., Sheng M.
    Nature 378:85-88(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DLG1; DLG2 AND DLG4.
  5. "N-glycosylation promotes the cell surface expression of Kv1.3 potassium channels."
    Zhu J., Yan J., Thornhill W.B.
    FEBS J. 279:2632-2644(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION AT ASN-229, ABSENCE OF GLYCOSYLATION AT ASN-228, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiKCNA3_RAT
AccessioniPrimary (citable) accession number: P15384
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: November 1, 1990
Last modified: September 3, 2014
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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