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P15384

- KCNA3_RAT

UniProt

P15384 - KCNA3_RAT

Protein

Potassium voltage-gated channel subfamily A member 3

Gene

Kcna3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 2 (01 Nov 1990)
      Previous versions | rss
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    Functioni

    Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei228 – 2281Not glycosylated

    GO - Molecular functioni

    1. delayed rectifier potassium channel activity Source: RGD
    2. outward rectifier potassium channel activity Source: RGD
    3. protein binding Source: IntAct
    4. voltage-gated potassium channel activity Source: RGD

    GO - Biological processi

    1. potassium ion transport Source: RGD
    2. protein homooligomerization Source: InterPro

    Keywords - Molecular functioni

    Ion channel, Potassium channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    Potassium

    Enzyme and pathway databases

    ReactomeiREACT_199159. Voltage gated Potassium channels.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Potassium voltage-gated channel subfamily A member 3
    Alternative name(s):
    RCK3
    RGK5
    Voltage-gated potassium channel subunit Kv1.3
    Voltage-gated potassium channel subunit Kv3
    Gene namesi
    Name:Kcna3
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 2

    Organism-specific databases

    RGDi2951. Kcna3.

    Subcellular locationi

    Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. membrane raft Source: BHF-UCL
    2. plasma membrane Source: BHF-UCL
    3. voltage-gated potassium channel complex Source: InterPro

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 525525Potassium voltage-gated channel subfamily A member 3PRO_0000053979Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi229 – 2291N-linked (GlcNAc...)1 Publication
    Lipidationi267 – 2671S-palmitoyl cysteineSequence Analysis
    Modified residuei470 – 4701Phosphoserine; by PKASequence Analysis

    Post-translational modificationi

    N-glycosylation promotes the cell surface expression.1 Publication

    Keywords - PTMi

    Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    PaxDbiP15384.
    PRIDEiP15384.

    Expressioni

    Gene expression databases

    GenevestigatoriP15384.

    Interactioni

    Subunit structurei

    Heterotetramer of potassium channel proteins By similarity. Binds PDZ domains of DLG1, DLG2 and DLG4.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    DLG4P783522EBI-631478,EBI-80389From a different organism.

    Protein-protein interaction databases

    IntActiP15384. 1 interaction.
    STRINGi10116.ENSRNOP00000024372.

    Structurei

    3D structure databases

    ProteinModelPortaliP15384.
    SMRiP15384. Positions 54-441.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 184184CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini204 – 24441ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini267 – 27711CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini299 – 31214ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini332 – 34716CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini368 – 40841ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini432 – 52594CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei185 – 20319Helical; Name=Segment S1Sequence AnalysisAdd
    BLAST
    Transmembranei245 – 26622Helical; Name=Segment S2Sequence AnalysisAdd
    BLAST
    Transmembranei278 – 29821Helical; Name=Segment S3Sequence AnalysisAdd
    BLAST
    Transmembranei313 – 33119Helical; Voltage-sensor; Name=Segment S4Sequence AnalysisAdd
    BLAST
    Transmembranei348 – 36720Helical; Name=Segment S5Sequence AnalysisAdd
    BLAST
    Transmembranei409 – 43123Helical; Name=Segment S6Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi394 – 3996Selectivity filterBy similarity
    Motifi523 – 5253PDZ-binding

    Domaini

    The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1226.
    GeneTreeiENSGT00740000115256.
    HOGENOMiHOG000231015.
    HOVERGENiHBG052230.
    InParanoidiP15384.
    KOiK04876.
    OMAiKRRMRYF.
    OrthoDBiEOG7M0NRD.
    PhylomeDBiP15384.
    TreeFamiTF313103.

    Family and domain databases

    Gene3Di1.20.120.350. 1 hit.
    3.30.710.10. 1 hit.
    InterProiIPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR027359. Channel_four-helix_dom.
    IPR005821. Ion_trans_dom.
    IPR003091. K_chnl.
    IPR003968. K_chnl_volt-dep_Kv.
    IPR003972. K_chnl_volt-dep_Kv1.
    IPR004050. K_chnl_volt-dep_Kv1.3.
    IPR003131. T1-type_BTB.
    IPR028325. VG_K_chnl.
    [Graphical view]
    PANTHERiPTHR11537. PTHR11537. 1 hit.
    PfamiPF02214. BTB_2. 1 hit.
    PF00520. Ion_trans. 1 hit.
    [Graphical view]
    PRINTSiPR00169. KCHANNEL.
    PR01510. KV13CHANNEL.
    PR01491. KVCHANNEL.
    PR01496. SHAKERCHANEL.
    SMARTiSM00225. BTB. 1 hit.
    [Graphical view]
    SUPFAMiSSF54695. SSF54695. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P15384-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTVVPGDHLL EPEAAGGGGG DPPQGGCVSG GGCDRYEPLP PALPAAGEQD    50
    CCGERVVINI SGLRFETQLK TLCQFPETLL GDPKRRMRYF DPLRNEYFFD 100
    RNRPSFDAIL YYYQSGGRIR RPVNVPIDIF SEEIRFYQLG EEAMEKFRED 150
    EGFLREEERP LPRRDFQRQV WLLFEYPESS GPARGIAIVS VLVILISIVI 200
    FCLETLPEFR DEKDYPASPS QDVFEAANNS TSGASSGASS FSDPFFVVET 250
    LCIIWFSFEL LVRFFACPSK ATFSRNIMNL IDIVAIIPYF ITLGTELAER 300
    QGNGQQAMSL AILRVIRLVR VFRIFKLSRH SKGLQILGQT LKASMRELGL 350
    LIFFLFIGVI LFSSAVYFAE ADDPSSGFNS IPDAFWWAVV TMTTVGYGDM 400
    HPVTIGGKIV GSLCAIAGVL TIALPVPVIV SNFNYFYHRE TEGEEQAQYM 450
    HVGSCQHLSS SAEELRKARS NSTLSKSEYM VIEEGGMNHS AFPQTPFKTG 500
    NSTATCTTNN NPNSCVNIKK IFTDV 525
    Length:525
    Mass (Da):58,425
    Last modified:November 1, 1990 - v2
    Checksum:i6DA8869D5471C401
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti106 – 1061F → L in CAA34132. (PubMed:2555158)Curated
    Sequence conflicti181 – 1811G → R in AAA42035. (PubMed:2351830)Curated
    Sequence conflicti430 – 4301V → L in AAA42035. (PubMed:2351830)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X16001 mRNA. Translation: CAA34132.1.
    M30312 Genomic DNA. Translation: AAA42035.1.
    M31744 Genomic DNA. Translation: AAA41500.1.
    PIRiA43531.
    RefSeqiNP_062143.3. NM_019270.3.
    UniGeneiRn.44292.

    Genome annotation databases

    EnsembliENSRNOT00000024372; ENSRNOP00000024372; ENSRNOG00000018116.
    GeneIDi29731.
    KEGGirno:29731.
    UCSCiRGD:2951. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X16001 mRNA. Translation: CAA34132.1 .
    M30312 Genomic DNA. Translation: AAA42035.1 .
    M31744 Genomic DNA. Translation: AAA41500.1 .
    PIRi A43531.
    RefSeqi NP_062143.3. NM_019270.3.
    UniGenei Rn.44292.

    3D structure databases

    ProteinModelPortali P15384.
    SMRi P15384. Positions 54-441.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P15384. 1 interaction.
    STRINGi 10116.ENSRNOP00000024372.

    Chemistry

    ChEMBLi CHEMBL4248.
    GuidetoPHARMACOLOGYi 540.

    Proteomic databases

    PaxDbi P15384.
    PRIDEi P15384.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000024372 ; ENSRNOP00000024372 ; ENSRNOG00000018116 .
    GeneIDi 29731.
    KEGGi rno:29731.
    UCSCi RGD:2951. rat.

    Organism-specific databases

    CTDi 3738.
    RGDi 2951. Kcna3.

    Phylogenomic databases

    eggNOGi COG1226.
    GeneTreei ENSGT00740000115256.
    HOGENOMi HOG000231015.
    HOVERGENi HBG052230.
    InParanoidi P15384.
    KOi K04876.
    OMAi KRRMRYF.
    OrthoDBi EOG7M0NRD.
    PhylomeDBi P15384.
    TreeFami TF313103.

    Enzyme and pathway databases

    Reactomei REACT_199159. Voltage gated Potassium channels.

    Miscellaneous databases

    NextBioi 610214.
    PROi P15384.

    Gene expression databases

    Genevestigatori P15384.

    Family and domain databases

    Gene3Di 1.20.120.350. 1 hit.
    3.30.710.10. 1 hit.
    InterProi IPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR027359. Channel_four-helix_dom.
    IPR005821. Ion_trans_dom.
    IPR003091. K_chnl.
    IPR003968. K_chnl_volt-dep_Kv.
    IPR003972. K_chnl_volt-dep_Kv1.
    IPR004050. K_chnl_volt-dep_Kv1.3.
    IPR003131. T1-type_BTB.
    IPR028325. VG_K_chnl.
    [Graphical view ]
    PANTHERi PTHR11537. PTHR11537. 1 hit.
    Pfami PF02214. BTB_2. 1 hit.
    PF00520. Ion_trans. 1 hit.
    [Graphical view ]
    PRINTSi PR00169. KCHANNEL.
    PR01510. KV13CHANNEL.
    PR01491. KVCHANNEL.
    PR01496. SHAKERCHANEL.
    SMARTi SM00225. BTB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54695. SSF54695. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular basis of functional diversity of voltage-gated potassium channels in mammalian brain."
      Stuehmer W., Ruppersberg J.P., Schroerter K.H., Sakmann B., Stocker M., Giese K.P., Perschke A., Baumann A., Pongs O.
      EMBO J. 8:3235-3244(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "Cloning and expression of cDNA and genomic clones encoding three delayed rectifier potassium channels in rat brain."
      Swanson R., Marshall J., Smith J., Williams J., Boyle M.B., Folander K., Luneau C.J., Antanavage J., Oliva C., Buhrow S.A., Bennett C., Stein R.B., Kaczmarek L.M.
      Neuron 4:929-939(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Brain.
    3. "Characterization and functional expression of a rat genomic DNA clone encoding a lymphocyte potassium channel."
      Douglass J., Osborne P.B., Cai Y.C., Wilkinson M., Christie M.J., Adelman J.P.
      J. Immunol. 144:4841-4850(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Lymphocyte.
    4. "Clustering of Shaker-type K+ channels by interaction with a family of membrane-associated guanylate kinases."
      Kim E., Niethammer M., Rothschild A., Jan Y.N., Sheng M.
      Nature 378:85-88(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH DLG1; DLG2 AND DLG4.
    5. "N-glycosylation promotes the cell surface expression of Kv1.3 potassium channels."
      Zhu J., Yan J., Thornhill W.B.
      FEBS J. 279:2632-2644(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION AT ASN-229, ABSENCE OF GLYCOSYLATION AT ASN-228, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiKCNA3_RAT
    AccessioniPrimary (citable) accession number: P15384
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: November 1, 1990
    Last modified: October 1, 2014
    This is version 128 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3