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Protein

Potassium voltage-gated channel subfamily E member 1

Gene

Kcne1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ancillary protein that assembles as a beta subunit with a voltage-gated potassium channel complex of pore-forming alpha subunits. Modulates the gating kinetics and enhances stability of the channel complex. Assembled with KCNB1 modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 (PubMed:19219384). Assembled with KCNQ1/KVLQT1 is proposed to form the slowly activating delayed rectifier cardiac potassium (IKs) channel. The outward current reaches its steady state only after 50 seconds. Assembled with KCNH2/HERG may modulate the rapidly activating component of the delayed rectifying potassium current in heart (IKr) (By similarity).By similarity1 Publication

Miscellaneous

Mutagenesis experiments were carried out by expressing in Xenopus oocytes the mutant Asn-77 either individually (homomultimers) or in combination with wild-type KCNE1 (heteromultimers) in a 1:1 ratio (PubMed:7605639).1 Publication

GO - Molecular functioni

  • delayed rectifier potassium channel activity Source: RGD
  • ion channel binding Source: UniProtKB
  • potassium channel regulator activity Source: UniProtKB
  • protein heterodimerization activity Source: RGD
  • telethonin binding Source: RGD
  • voltage-gated potassium channel activity Source: RGD

GO - Biological processi

Keywordsi

Molecular functionIon channel, Potassium channel, Voltage-gated channel
Biological processIon transport, Potassium transport, Transport
LigandPotassium

Enzyme and pathway databases

ReactomeiR-RNO-5576890. Phase 3 - rapid repolarisation.
R-RNO-5576893. Phase 2 - plateau phase.

Protein family/group databases

TCDBi8.A.10.1.1. the slow voltage-gated k+) channel accessory protein (mink) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium voltage-gated channel subfamily E member 1
Alternative name(s):
Delayed rectifier potassium channel subunit IsK
IKs producing slow voltage-gated potassium channel subunit beta Mink
Minimal potassium channel
Gene namesi
Name:Kcne1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 11

Organism-specific databases

RGDi2956. Kcne1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei45 – 67HelicalSequence analysisAdd BLAST23
Topological domaini68 – 130CytoplasmicSequence analysisAdd BLAST63

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi77D → N: No current (homomultimers); one fourth of the WT current (heteromultimers). 1 Publication1
Mutagenesisi103S → A: Abolishes inhibition by phosphorylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001442831 – 130Potassium voltage-gated channel subfamily E member 1Add BLAST130

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi5N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi26N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei103Phosphoserine; by PKCCurated1

Post-translational modificationi

Phosphorylation inhibits the potassium current.
N-glycosylation at Asn-26 occurs post-translationally, and requires prior cotranslational glycosylation at Asn-5.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP15383.

PTM databases

iPTMnetiP15383.
PhosphoSitePlusiP15383.

Expressioni

Tissue specificityi

Expressed in the heart (PubMed:2183220, PubMed:19219384). Expressed in kidney (PubMed:2344412). Expressed in estrogen-induced uterus (PubMed:2344412).3 Publications

Inductioni

By estrogen.1 Publication

Gene expression databases

BgeeiENSRNOG00000001984.
GenevisibleiP15383. RN.

Interactioni

Subunit structurei

Interacts with KCNB1 (PubMed:19219384). Interacts with KCNC2 (PubMed:14679187). Associates with KCNH2/HERG (By similarity). Interacts with KNCQ1; targets the complex KNCQ1-KCNE1 to the membrane raft (By similarity).By similarity2 Publications

GO - Molecular functioni

  • ion channel binding Source: UniProtKB
  • protein heterodimerization activity Source: RGD
  • telethonin binding Source: RGD

Protein-protein interaction databases

CORUMiP15383.
STRINGi10116.ENSRNOP00000002717.

Structurei

3D structure databases

SMRiP15383.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the potassium channel KCNE family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IY71. Eukaryota.
ENOG410Y1SF. LUCA.
GeneTreeiENSGT00510000048994.
HOGENOMiHOG000113207.
HOVERGENiHBG052226.
InParanoidiP15383.
KOiK04894.
OMAiALYILMV.
OrthoDBiEOG091G0UDJ.
PhylomeDBiP15383.
TreeFamiTF335976.

Family and domain databases

InterProiView protein in InterPro
IPR000369. K_chnl_KCNE.
IPR005424. K_chnl_volt-dep_bsu_KCNE1.
PANTHERiPTHR15282. PTHR15282. 1 hit.
PfamiView protein in Pfam
PF02060. ISK_Channel. 1 hit.
PRINTSiPR01604. KCNE1CHANNEL.
PR00168. KCNECHANNEL.

Sequencei

Sequence statusi: Complete.

P15383-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALSNSTTVL PFLASLWQET DEPGGNMSAD LARRSQLRDD SKLEALYILM
60 70 80 90 100
VLGFFGFFTL GIMLSYIRSK KLEHSHDPFN VYIESDAWQE KGKALFQARV
110 120 130
LESFRACYVI ENQAAVEQPA THLPELKPLS
Length:130
Mass (Da):14,699
Last modified:April 1, 1990 - v1
Checksum:i76717E7ED73C5E4D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22412 mRNA. Translation: AAA41822.1.
M36461 mRNA. Translation: AAA41098.1.
D10709 Genomic DNA. Translation: BAA01553.1. Sequence problems.
PIRiA35633.
RefSeqiNP_037105.1. NM_012973.1.
XP_006248096.1. XM_006248034.3.
XP_006248097.1. XM_006248035.2.
XP_008766788.1. XM_008768566.2.
XP_017453375.1. XM_017597886.1.
XP_017453376.1. XM_017597887.1.
UniGeneiRn.9734.

Genome annotation databases

EnsembliENSRNOT00000002717; ENSRNOP00000002717; ENSRNOG00000001984.
GeneIDi25471.
KEGGirno:25471.
UCSCiRGD:2956. rat.

Similar proteinsi

Entry informationi

Entry nameiKCNE1_RAT
AccessioniPrimary (citable) accession number: P15383
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: November 22, 2017
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families