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Protein

Potassium voltage-gated channel subfamily E member 1

Gene

Kcne1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ancillary protein that assembles as a beta subunit with a voltage-gated potassium channel complex of pore-forming alpha subunits. Modulates the gating kinetics and enhances stability of the channel complex. Assembled with KCNB1 modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 (PubMed:19219384). Assembled with KCNQ1/KVLQT1 is proposed to form the slowly activating delayed rectifier cardiac potassium (IKs) channel. The outward current reaches its steady state only after 50 seconds. Assembled with KCNH2/HERG may modulate the rapidly activating component of the delayed rectifying potassium current in heart (IKr) (By similarity).By similarity1 Publication

GO - Molecular functioni

  • delayed rectifier potassium channel activity Source: UniProtKB
  • ion channel binding Source: UniProtKB
  • potassium channel regulator activity Source: UniProtKB
  • protein heterodimerization activity Source: RGD
  • voltage-gated potassium channel activity Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiR-RNO-5576890. Phase 3 - rapid repolarisation.
R-RNO-5576893. Phase 2 - plateau phase.

Protein family/group databases

TCDBi8.A.10.1.1. the slow voltage-gated k+) channel accessory protein (mink) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium voltage-gated channel subfamily E member 1
Alternative name(s):
Delayed rectifier potassium channel subunit IsK
IKs producing slow voltage-gated potassium channel subunit beta Mink
Minimal potassium channel
Gene namesi
Name:Kcne1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 11

Organism-specific databases

RGDi2956. Kcne1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei45 – 6723HelicalSequence analysisAdd
BLAST
Topological domaini68 – 13063CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • apical plasma membrane Source: RGD
  • endoplasmic reticulum Source: RGD
  • membrane raft Source: UniProtKB-SubCell
  • plasma membrane Source: UniProtKB
  • voltage-gated potassium channel complex Source: RGD
  • Z disc Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi77 – 771D → N: No current (homomultimers); one fourth of the WT current (heteromultimers). 1 Publication
Mutagenesisi103 – 1031S → A: Abolishes inhibition by phosphorylation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 130130Potassium voltage-gated channel subfamily E member 1PRO_0000144283Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi5 – 51N-linked (GlcNAc...)Sequence analysis
Glycosylationi26 – 261N-linked (GlcNAc...)Sequence analysis
Modified residuei103 – 1031Phosphoserine; by PKCCurated

Post-translational modificationi

Phosphorylation inhibits the potassium current.
N-glycosylation at Asn-26 occurs post-translationally, and requires prior cotranslational glycosylation at Asn-5.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP15383.

PTM databases

iPTMnetiP15383.
PhosphoSiteiP15383.

Expressioni

Tissue specificityi

Expressed in the heart (PubMed:2183220, PubMed:19219384). Expressed in kidney (PubMed:2344412). Expressed in estrogen-induced uterus (PubMed:2344412).3 Publications

Inductioni

By estrogen.1 Publication

Gene expression databases

GenevisibleiP15383. RN.

Interactioni

Subunit structurei

Interacts with KCNB1 (PubMed:19219384). Interacts with KCNC2 (PubMed:14679187). Associates with KCNH2/HERG (By similarity). Interacts with KNCQ1; targets the complex KNCQ1-KCNE1 to the membrane raft (By similarity).By similarity2 Publications

GO - Molecular functioni

  • ion channel binding Source: UniProtKB
  • protein heterodimerization activity Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000002717.

Family & Domainsi

Sequence similaritiesi

Belongs to the potassium channel KCNE family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IY71. Eukaryota.
ENOG410Y1SF. LUCA.
GeneTreeiENSGT00510000048994.
HOGENOMiHOG000113207.
HOVERGENiHBG052226.
InParanoidiP15383.
KOiK04894.
OMAiALYILMV.
OrthoDBiEOG7WMCMH.
PhylomeDBiP15383.
TreeFamiTF335976.

Family and domain databases

InterProiIPR000369. K_chnl_KCNE.
IPR005424. K_chnl_volt-dep_bsu_KCNE1.
[Graphical view]
PfamiPF02060. ISK_Channel. 1 hit.
[Graphical view]
PRINTSiPR01604. KCNE1CHANNEL.
PR00168. KCNECHANNEL.

Sequencei

Sequence statusi: Complete.

P15383-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALSNSTTVL PFLASLWQET DEPGGNMSAD LARRSQLRDD SKLEALYILM
60 70 80 90 100
VLGFFGFFTL GIMLSYIRSK KLEHSHDPFN VYIESDAWQE KGKALFQARV
110 120 130
LESFRACYVI ENQAAVEQPA THLPELKPLS
Length:130
Mass (Da):14,699
Last modified:April 1, 1990 - v1
Checksum:i76717E7ED73C5E4D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22412 mRNA. Translation: AAA41822.1.
M36461 mRNA. Translation: AAA41098.1.
D10709 Genomic DNA. Translation: BAA01553.1. Sequence problems.
PIRiA35633.
RefSeqiNP_037105.1. NM_012973.1.
XP_006248096.1. XM_006248034.2.
XP_006248097.1. XM_006248035.2.
XP_008766788.1. XM_008768566.1.
UniGeneiRn.9734.

Genome annotation databases

EnsembliENSRNOT00000002717; ENSRNOP00000002717; ENSRNOG00000001984.
GeneIDi25471.
KEGGirno:25471.
UCSCiRGD:2956. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22412 mRNA. Translation: AAA41822.1.
M36461 mRNA. Translation: AAA41098.1.
D10709 Genomic DNA. Translation: BAA01553.1. Sequence problems.
PIRiA35633.
RefSeqiNP_037105.1. NM_012973.1.
XP_006248096.1. XM_006248034.2.
XP_006248097.1. XM_006248035.2.
XP_008766788.1. XM_008768566.1.
UniGeneiRn.9734.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000002717.

Protein family/group databases

TCDBi8.A.10.1.1. the slow voltage-gated k+) channel accessory protein (mink) family.

PTM databases

iPTMnetiP15383.
PhosphoSiteiP15383.

Proteomic databases

PaxDbiP15383.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000002717; ENSRNOP00000002717; ENSRNOG00000001984.
GeneIDi25471.
KEGGirno:25471.
UCSCiRGD:2956. rat.

Organism-specific databases

CTDi3753.
RGDi2956. Kcne1.

Phylogenomic databases

eggNOGiENOG410IY71. Eukaryota.
ENOG410Y1SF. LUCA.
GeneTreeiENSGT00510000048994.
HOGENOMiHOG000113207.
HOVERGENiHBG052226.
InParanoidiP15383.
KOiK04894.
OMAiALYILMV.
OrthoDBiEOG7WMCMH.
PhylomeDBiP15383.
TreeFamiTF335976.

Enzyme and pathway databases

ReactomeiR-RNO-5576890. Phase 3 - rapid repolarisation.
R-RNO-5576893. Phase 2 - plateau phase.

Miscellaneous databases

PROiP15383.

Gene expression databases

GenevisibleiP15383. RN.

Family and domain databases

InterProiIPR000369. K_chnl_KCNE.
IPR005424. K_chnl_volt-dep_bsu_KCNE1.
[Graphical view]
PfamiPF02060. ISK_Channel. 1 hit.
[Graphical view]
PRINTSiPR01604. KCNE1CHANNEL.
PR00168. KCNECHANNEL.
ProtoNetiSearch...

Publicationsi

  1. "Cloning of a membrane protein that induces a slow voltage-gated potassium current."
    Takumi T., Ohkubo H., Nakanishi S.
    Science 242:1042-1045(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney.
  2. "Cloning and expression of the delayed-rectifier IsK channel from neonatal rat heart and diethylstilbestrol-primed rat uterus."
    Folander K., Smith J.S., Antanavage J., Bennett C., Stein R.B., Swanson R.
    Proc. Natl. Acad. Sci. U.S.A. 87:2975-2979(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Heart.
  3. "Estrogen induction of a small, putative K+ channel mRNA in rat uterus."
    Pragnell M., Snay K.J., Trimmer J.S., Maclusky N.J., Naftolin F., Kaczmarek L.K., Boyle M.B.
    Neuron 4:807-812(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE, INDUCTION, TISSUE SPECIFICITY.
    Tissue: Uterus.
  4. "Characterization of gene organization and generation of heterogeneous mRNA species of rat ISK protein."
    Iwai M., Masu M., Tsuchida K., Mori T., Ohkubo H., Nakanishi S.
    J. Biochem. 108:200-206(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  5. "An amino acid mutation in a potassium channel that prevents inhibition by protein kinase C."
    Busch A.E., Varnum M.D., North R.A., Adelman J.P.
    Science 255:1705-1707(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF SER-103.
  6. "Subunit composition of minK potassium channels."
    Wang K.-W., Goldstein S.A.N.
    Neuron 14:1303-1309(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ASP-77.
  7. "MinK, MiRP1, and MiRP2 diversify Kv3.1 and Kv3.2 potassium channel gating."
    Lewis A., McCrossan Z.A., Abbott G.W.
    J. Biol. Chem. 279:7884-7892(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, INTERACTION WITH KCNC2, SUBCELLULAR LOCATION.
  8. "Regulation of the Kv2.1 potassium channel by MinK and MiRP1."
    McCrossan Z.A., Roepke T.K., Lewis A., Panaghie G., Abbott G.W.
    J. Membr. Biol. 228:1-14(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH KCNB1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiKCNE1_RAT
AccessioniPrimary (citable) accession number: P15383
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: June 8, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Mutagenesis experiments were carried out by expressing in Xenopus oocytes the mutant Asn-77 either individually (homomultimers) or in combination with wild-type KCNE1 (heteromultimers) in a 1:1 ratio (PubMed:7605639).1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.