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Protein

Proline-specific permease

Gene

PUT4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Required for high-affinity proline transport. May be responsible for proline recognition and probably also for proline translocation across the plasma membrane. Also function as non-specific GABA permease. Can also transport alanine and glycine.1 Publication

GO - Molecular functioni

  • antiporter activity Source: GO_Central
  • L-proline transmembrane transporter activity Source: SGD
  • neutral amino acid transmembrane transporter activity Source: SGD

GO - Biological processi

  • gamma-aminobutyric acid transport Source: SGD
  • neutral amino acid transport Source: SGD
  • proline transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33820-MONOMER.

Protein family/group databases

TCDBi2.A.3.10.3. the amino acid-polyamine-organocation (apc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Proline-specific permease
Gene namesi
Name:PUT4
Ordered Locus Names:YOR348C
ORF Names:O6345
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR348C.
SGDiS000005875. PUT4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei115 – 136HelicalSequence analysisAdd BLAST22
Transmembranei142 – 162HelicalSequence analysisAdd BLAST21
Transmembranei191 – 210HelicalSequence analysisAdd BLAST20
Transmembranei229 – 247HelicalSequence analysisAdd BLAST19
Transmembranei262 – 281HelicalSequence analysisAdd BLAST20
Transmembranei306 – 326HelicalSequence analysisAdd BLAST21
Transmembranei351 – 370HelicalSequence analysisAdd BLAST20
Transmembranei408 – 427HelicalSequence analysisAdd BLAST20
Transmembranei455 – 473HelicalSequence analysisAdd BLAST19
Transmembranei483 – 503HelicalSequence analysisAdd BLAST21
Transmembranei522 – 542HelicalSequence analysisAdd BLAST21
Transmembranei558 – 578HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: SGD
  • vacuole Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000541581 – 627Proline-specific permeaseAdd BLAST627

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi72N-linked (GlcNAc...)Sequence analysis1
Glycosylationi78N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP15380.

PTM databases

iPTMnetiP15380.

Expressioni

Inductioni

Requires the presence of GABA.

Interactioni

Protein-protein interaction databases

BioGridi34733. 30 interactors.
DIPiDIP-1541N.
IntActiP15380. 6 interactors.
MINTiMINT-394548.

Structurei

3D structure databases

ProteinModelPortaliP15380.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00510000049744.
HOGENOMiHOG000261848.
InParanoidiP15380.
KOiK16261.
OMAiSIKILCI.
OrthoDBiEOG092C164R.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR002293. AA/rel_permease1.
IPR004762. Amino_acid_permease_fungi.
IPR004840. Amoino_acid_permease_CS.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF00324. AA_permease. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00913. 2A0310. 1 hit.
PROSITEiPS00218. AMINO_ACID_PERMEASE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P15380-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNILPFHKN NRHSAGVVTC ADDVSGDGSG GDTKKEEDVV QVTESPSSGS
60 70 80 90 100
RNNHRSDNEK DDAIRMEKIS KNQSASSNGT IREDLIMDVD LEKSPSVDGD
110 120 130 140 150
SEPHKLKQGL QSRHVQLIAL GGAIGTGLLV GTSSTLHTCG PAGLFISYII
160 170 180 190 200
ISAVIYPIMC ALGEMVCFLP GDGSDSAGST ANLVTRYVDP SLGFATGWNY
210 220 230 240 250
FYCYVILVAA ECTAASGVVE YWTTAVPKGV WITIFLCVVV ILNFSAVKVY
260 270 280 290 300
GESEFWFASI KILCIVGLII LSFILFWGGG PNHDRLGFRY WQHPGAFAHH
310 320 330 340 350
LTGGSLGNFT DIYTGIIKGA FAFILGPELV CMTSAECADQ RRNIAKASRR
360 370 380 390 400
FVWRLIFFYV LGTLAISVIV PYNDPTLVNA LAQGKPGAGS SPFVIGIQNA
410 420 430 440 450
GIKVLPHIIN GCILTSAWSA ANAFMFASTR SLLTMAQTGQ APKCLGRINK
460 470 480 490 500
WGVPYVAVGV SFLCSCLAYL NVSSSTADVF NWFSNISTIS GFLGWMCGCI
510 520 530 540 550
AYLRFRKAIF YNGLYDRLPF KTWGQPYTVW FSLIVIGIIT ITNGYAIFIP
560 570 580 590 600
KYWRVADFIA AYITLPIFLV LWFGHKLYTR TWRQWWLPVS EIDVTTGLVE
610 620
IEEKSREIEE MRLPPTGFKD KFLDALL
Length:627
Mass (Da):68,788
Last modified:October 1, 1996 - v2
Checksum:iBC76CBA24BB417BD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti38D → N in AAA34925 (PubMed:2687114).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30583 Genomic DNA. Translation: AAA34925.1.
X95720 Genomic DNA. Translation: CAA65035.1.
Z75256 Genomic DNA. Translation: CAA99676.1.
BK006948 Genomic DNA. Translation: DAA11109.1.
PIRiS67257.
RefSeqiNP_014993.1. NM_001183768.1.

Genome annotation databases

EnsemblFungiiYOR348C; YOR348C; YOR348C.
GeneIDi854530.
KEGGisce:YOR348C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30583 Genomic DNA. Translation: AAA34925.1.
X95720 Genomic DNA. Translation: CAA65035.1.
Z75256 Genomic DNA. Translation: CAA99676.1.
BK006948 Genomic DNA. Translation: DAA11109.1.
PIRiS67257.
RefSeqiNP_014993.1. NM_001183768.1.

3D structure databases

ProteinModelPortaliP15380.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34733. 30 interactors.
DIPiDIP-1541N.
IntActiP15380. 6 interactors.
MINTiMINT-394548.

Protein family/group databases

TCDBi2.A.3.10.3. the amino acid-polyamine-organocation (apc) family.

PTM databases

iPTMnetiP15380.

Proteomic databases

PRIDEiP15380.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR348C; YOR348C; YOR348C.
GeneIDi854530.
KEGGisce:YOR348C.

Organism-specific databases

EuPathDBiFungiDB:YOR348C.
SGDiS000005875. PUT4.

Phylogenomic databases

GeneTreeiENSGT00510000049744.
HOGENOMiHOG000261848.
InParanoidiP15380.
KOiK16261.
OMAiSIKILCI.
OrthoDBiEOG092C164R.

Enzyme and pathway databases

BioCyciYEAST:G3O-33820-MONOMER.

Miscellaneous databases

PROiP15380.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR002293. AA/rel_permease1.
IPR004762. Amino_acid_permease_fungi.
IPR004840. Amoino_acid_permease_CS.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF00324. AA_permease. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00913. 2A0310. 1 hit.
PROSITEiPS00218. AMINO_ACID_PERMEASE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPUT4_YEAST
AccessioniPrimary (citable) accession number: P15380
Secondary accession number(s): D6W343
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.