Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Proline-specific permease

Gene

PUT4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Required for high-affinity proline transport. May be responsible for proline recognition and probably also for proline translocation across the plasma membrane. Also function as non-specific GABA permease. Can also transport alanine and glycine.1 Publication

GO - Molecular functioni

  • L-proline transmembrane transporter activity Source: SGD
  • neutral amino acid transmembrane transporter activity Source: SGD

GO - Biological processi

  • gamma-aminobutyric acid transport Source: SGD
  • neutral amino acid transport Source: SGD
  • proline transmembrane transport Source: GOC
  • proline transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33820-MONOMER.

Protein family/group databases

TCDBi2.A.3.10.3. the amino acid-polyamine-organocation (apc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Proline-specific permease
Gene namesi
Name:PUT4
Ordered Locus Names:YOR348C
ORF Names:O6345
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR348C.
SGDiS000005875. PUT4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei115 – 13622HelicalSequence analysisAdd
BLAST
Transmembranei142 – 16221HelicalSequence analysisAdd
BLAST
Transmembranei191 – 21020HelicalSequence analysisAdd
BLAST
Transmembranei229 – 24719HelicalSequence analysisAdd
BLAST
Transmembranei262 – 28120HelicalSequence analysisAdd
BLAST
Transmembranei306 – 32621HelicalSequence analysisAdd
BLAST
Transmembranei351 – 37020HelicalSequence analysisAdd
BLAST
Transmembranei408 – 42720HelicalSequence analysisAdd
BLAST
Transmembranei455 – 47319HelicalSequence analysisAdd
BLAST
Transmembranei483 – 50321HelicalSequence analysisAdd
BLAST
Transmembranei522 – 54221HelicalSequence analysisAdd
BLAST
Transmembranei558 – 57821HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 627627Proline-specific permeasePRO_0000054158Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi72 – 721N-linked (GlcNAc...)Sequence analysis
Glycosylationi78 – 781N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PeptideAtlasiP15380.

PTM databases

iPTMnetiP15380.

Expressioni

Inductioni

Requires the presence of GABA.

Interactioni

Protein-protein interaction databases

BioGridi34733. 30 interactions.
DIPiDIP-1541N.
IntActiP15380. 6 interactions.
MINTiMINT-394548.

Structurei

3D structure databases

ProteinModelPortaliP15380.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00510000049744.
HOGENOMiHOG000261848.
InParanoidiP15380.
KOiK16261.
OMAiFNWFSNI.
OrthoDBiEOG74J9HZ.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR002293. AA/rel_permease1.
IPR004762. Amino_acid_permease_fungi.
IPR004840. Amoino_acid_permease_CS.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF00324. AA_permease. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00913. 2A0310. 1 hit.
PROSITEiPS00218. AMINO_ACID_PERMEASE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P15380-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNILPFHKN NRHSAGVVTC ADDVSGDGSG GDTKKEEDVV QVTESPSSGS
60 70 80 90 100
RNNHRSDNEK DDAIRMEKIS KNQSASSNGT IREDLIMDVD LEKSPSVDGD
110 120 130 140 150
SEPHKLKQGL QSRHVQLIAL GGAIGTGLLV GTSSTLHTCG PAGLFISYII
160 170 180 190 200
ISAVIYPIMC ALGEMVCFLP GDGSDSAGST ANLVTRYVDP SLGFATGWNY
210 220 230 240 250
FYCYVILVAA ECTAASGVVE YWTTAVPKGV WITIFLCVVV ILNFSAVKVY
260 270 280 290 300
GESEFWFASI KILCIVGLII LSFILFWGGG PNHDRLGFRY WQHPGAFAHH
310 320 330 340 350
LTGGSLGNFT DIYTGIIKGA FAFILGPELV CMTSAECADQ RRNIAKASRR
360 370 380 390 400
FVWRLIFFYV LGTLAISVIV PYNDPTLVNA LAQGKPGAGS SPFVIGIQNA
410 420 430 440 450
GIKVLPHIIN GCILTSAWSA ANAFMFASTR SLLTMAQTGQ APKCLGRINK
460 470 480 490 500
WGVPYVAVGV SFLCSCLAYL NVSSSTADVF NWFSNISTIS GFLGWMCGCI
510 520 530 540 550
AYLRFRKAIF YNGLYDRLPF KTWGQPYTVW FSLIVIGIIT ITNGYAIFIP
560 570 580 590 600
KYWRVADFIA AYITLPIFLV LWFGHKLYTR TWRQWWLPVS EIDVTTGLVE
610 620
IEEKSREIEE MRLPPTGFKD KFLDALL
Length:627
Mass (Da):68,788
Last modified:October 1, 1996 - v2
Checksum:iBC76CBA24BB417BD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti38 – 381D → N in AAA34925 (PubMed:2687114).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30583 Genomic DNA. Translation: AAA34925.1.
X95720 Genomic DNA. Translation: CAA65035.1.
Z75256 Genomic DNA. Translation: CAA99676.1.
BK006948 Genomic DNA. Translation: DAA11109.1.
PIRiS67257.
RefSeqiNP_014993.1. NM_001183768.1.

Genome annotation databases

EnsemblFungiiYOR348C; YOR348C; YOR348C.
GeneIDi854530.
KEGGisce:YOR348C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30583 Genomic DNA. Translation: AAA34925.1.
X95720 Genomic DNA. Translation: CAA65035.1.
Z75256 Genomic DNA. Translation: CAA99676.1.
BK006948 Genomic DNA. Translation: DAA11109.1.
PIRiS67257.
RefSeqiNP_014993.1. NM_001183768.1.

3D structure databases

ProteinModelPortaliP15380.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34733. 30 interactions.
DIPiDIP-1541N.
IntActiP15380. 6 interactions.
MINTiMINT-394548.

Protein family/group databases

TCDBi2.A.3.10.3. the amino acid-polyamine-organocation (apc) family.

PTM databases

iPTMnetiP15380.

Proteomic databases

PeptideAtlasiP15380.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR348C; YOR348C; YOR348C.
GeneIDi854530.
KEGGisce:YOR348C.

Organism-specific databases

EuPathDBiFungiDB:YOR348C.
SGDiS000005875. PUT4.

Phylogenomic databases

GeneTreeiENSGT00510000049744.
HOGENOMiHOG000261848.
InParanoidiP15380.
KOiK16261.
OMAiFNWFSNI.
OrthoDBiEOG74J9HZ.

Enzyme and pathway databases

BioCyciYEAST:G3O-33820-MONOMER.

Miscellaneous databases

PROiP15380.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR002293. AA/rel_permease1.
IPR004762. Amino_acid_permease_fungi.
IPR004840. Amoino_acid_permease_CS.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF00324. AA_permease. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00913. 2A0310. 1 hit.
PROSITEiPS00218. AMINO_ACID_PERMEASE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the Saccharomyces cerevisiae PUT4 proline-permease-encoding gene: similarities between CAN1, HIP1 and PUT4 permeases."
    Vandenbol M., Jauniaux J.-C., Grenson M.
    Gene 83:153-159(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Nucleotide sequence analysis of a 40 kb segment on the right arm of yeast chromosome XV reveals 18 open reading frames including a new pyruvate kinase and three homologues to chromosome I genes."
    Purnelle B., Goffeau A.
    Yeast 12:1475-1481(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 90843 / S288c / FY73.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Substrate specificity and gene expression of the amino-acid permeases in Saccharomyces cerevisiae."
    Regenberg B., During-Olsen L., Kielland-Brandt M.C., Holmberg S.
    Curr. Genet. 36:317-328(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPUT4_YEAST
AccessioniPrimary (citable) accession number: P15380
Secondary accession number(s): D6W343
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: October 1, 1996
Last modified: June 8, 2016
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.